From phyllosphere to insect cuticles: silkworms gather antifungal bacteria from mulberry leaves to battle fungal parasite attacks

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: Feb. 26, 2024

Abstract Background Bacterial transfers from plants to insect herbivore guts have been well investigated. However, bacterial exchanges between plant phyllospheres and cuticles remain unclear, as does their related biological function. Results Here, we report that the cuticular loads of silkworm larvae quickly increased after molting feeding on white mulberry ( Morus alba ) leaves. The isolation examination bacteria identified one bacterium Mammaliicoccus sciuri could completely inhibit spore germination fungal entomopathogens Metarhizium robertsii Beauveria bassiana . Interestingly, Ma. was evident originally leaves, which produce a secreted chitinolytic lysozyme (termed Msp1) damage cell walls. In consistency, deletion Msp1 substantially impaired antifungal activity. Pretreating with cells followed by topical infections revealed this help defend silkworms against infections. Unsurprisingly, protective efficacy Δ considerably reduced when compared wild-type bacterium. Administration bacterium-treated diets had no negative effect development; instead, supplementation protect artificial diet Aspergillus contamination. Conclusions results study evidence cross-kingdom transfer can insects parasite attacks.

Language: Английский

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Co-habiting ants and silverfish display a converging feeding ecology

BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 28, 2024

Various animal taxa have specialized to living with social hosts. Depending on their level of specialization, these symbiotic animals are characterized by distinct behavioural, chemical, and morphological traits that enable close heterospecific interactions. Despite its functional importance, our understanding the feeding ecology hosts remains limited. We examined how host specialization silverfish co-habiting ants affects several components ecology. combined stable isotope profiling, assays, phylogenetic reconstruction, microbial community characterization Neoasterolepisma genus a wider nicoletiid lepismatid panel where divergent myrmecophilous lifestyles observed.

Language: Английский

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The plant-sucking insect selects assembly of the gut microbiota from environment to enhance host reproduction

npj Biofilms and Microbiomes, Journal Year: 2024, Volume and Issue: 10(1)

Published: July 30, 2024

Abstract Plant-sucking insects have intricate associations with a diverse array of microorganisms to facilitate their adaptation specific ecological niches. The midgut phytophagous true bugs is generally structured into four distinct compartments accommodate microbiota. Nevertheless, there limited understanding regarding the origins these gut microbiomes, mechanisms behind microbial community assembly, and interactions between microbiomes insect hosts. In this study, we conducted comprehensive survey communities within bean bug Riptortus pedestris , soybean plant, bulk soil across 12 geographical fields in China, utilizing high-throughput sequencing 16 S rRNA gene. Our findings illuminated that microbiota plant-sucking predominantly originated from surrounding environment, plants also play subordinate role mediating acquisition for insects. Furthermore, our investigation suggested composition microbiome was probably shaped by host selection and/or microbe-microbe at compartment level, marginal influence factors. Additionally, had unveiled noteworthy dynamic core bacterial taxa, particularly Burkholderia which were initially sourced environment subsequently enriched compartments. This enrichment played significant enhancing reproduction. These contribute evolving insect-plant-soil ecosystem, shedding additional light on underpin significance partnerships adaptation.

Language: Английский

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Soil microbiota and herbivory drive the assembly of tomato plant-associated microbial communities through different mechanisms

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: May 13, 2024

Abstract Plant-associated microbial communities are key to shaping many aspects of plant biology. In this study, we tested whether soil and herbivory influence the bacterial community tomato plants their in different compartments is driven by spillover between or involved mediating effect. We grew our soils hosting three covered (or not) surface prevent allow) passive compartments, exposed them Manduca sexta . Here show that soil-driven effect on aboveground consistently detected regardless coverage, whereas cover influences herbivore-driven belowground microbiota. Together, results suggest microbiota insect via changes metabolism physiology sharing microorganisms xylem sap. contrast, herbivores a combination metabolism. These demonstrate important role linking microbiota, can foster further research manipulation for sustainable pest management.

Language: Английский

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The floral interface: a playground for interactions between insect pollinators, microbes, and plants

New Zealand Journal of Zoology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 20

Published: May 23, 2024

Insect pollinators acquire microorganisms when they visit flowers for nutrients. The interactions that occur at the floral interface are complex with three participants – pollinators, plants and microorganisms. majority of insect pollinator's microbiome is shaped by their behaviour, diet environment. At present bee (Apidae family) best documented contributes to our understanding bi-directional exchange microbes between flowers. transferred may be mutualistic, commensal or pathogenic. We identify a lack information due limited studies concerning diversity focus on pathogenic gut influence health. Each candidate, insect, plant microbe, makes own contribution which aids interaction, but some benefit more than others. benefits include enhanced acquisition nutritional resources; dispersal 'new' habitat colonise pollination outcome. Finally, we explore novel concept whether fruit acts as potential vector hibernate extend lifecycle in absence pollinator host.

Language: Английский

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Microbial changes and associated metabolic responses modify host plant adaptation in Stephanitis nashi

Insect Science, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 18, 2024

Abstract Symbiotic microorganisms are essential for the physiological processes of herbivorous pests, including pear lace bug Stephanitis nashi , which is known causing extensive damage to garden plants and fruit trees due its exceptional adaptability diverse host plants. However, specific functional effects microbiome on adaptation S. remains unclear. Here, we identified significant microbial changes in 2 different plants, crabapple cherry blossom, characterized by differences fungal diversity as well bacterial community structures, with abundant correlations between bacteria or fungi. Consistent changes, that fed blossom demonstrated decreased metabolites downregulated key metabolic pathways, such arginine mitogen‐activated protein kinase signaling pathway, were crucial plant adaptation. Furthermore, correlation analysis unveiled numerous differential metabolites, influenced interactions These bacteria, fungi, associated may modify pathways aiding results provide valuable insights into alteration function adapted contributing a better understanding pest invasion dispersal from perspective.

Language: Английский

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Gut bacteria of Spodoptera frugiperda establish endophytic association and affect the interactions of their host herbivore with maize plants

Journal of Pest Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Language: Английский

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The role of host plants in driving pathogen susceptibility in insects through chemicals, immune responses and microbiota

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

In this comprehensive exploration, we delve into the pivotal role of host plants in shaping intricate interactions between herbivorous insects and their pathogens. Recent decades have seen a surge studies that demonstrate are crucial drivers pathogens, providing novel insights direct indirect shape tri-trophic interactions. These built on wide range from viruses to bacteria, protozoans fungi. We summarise these studies, discuss mechanisms plant-mediated insect resistance infection, ranging toxicity plant chemicals pathogens enhancement anti-pathogen immune responses, modulation insect's microbiome. Although provide evidence for roles all mechanisms, also point out majority existing phenomenological, describing patterns without addressing underlying mechanisms. To further our understanding interactions, therefore urge researchers design enable them specifically distinguish by which affect susceptibility

Language: Английский

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Positive Linkage in Bacterial Microbiota at the Plant‐Insect Interface Benefits an Invasive Bark Beetle

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

ABSTRACT Symbiotic microbes facilitate rapid adaptation of invasive insects on novel plants via multifaceted function provisions, but little was known the importance cross linkages in symbiotic microbiota to insect invasiveness. Novel host pine Pinus tabuliformis is inherently unsuitable for red turpentine beetle (RTB) China; however, Novosphingobium and Erwinia / Serratia gallery (at interface between RTB larvae phloem) have been discovered help beetles biodegrading detrimental compounds naringenin pinitol, respectively. Here, we further revealed significant positive linkage two functions, with higher activity level conferring more growth benefit larvae. Abundance remarkably increased response while naringenin‐biodegrading unable utilize this main phloem carbohydrate directly. High‐activity bacterial produced nutritive metabolites (sucrose hexadecanoic acid) from pinitol consumption that facilitated both Functional proteins several taxa were enriched high‐activity appeared form a metabolic network collectively regulate nutrient production. Our results indicate interaction critical invasion success, Bacilli bacteria might restrict linkage, providing new insights into microbial interactions herbivores.

Language: Английский

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Bacterial dynamics and exchange in plant-insect interactions

Current Research in Insect Science, Journal Year: 2025, Volume and Issue: unknown, P. 100110 - 100110

Published: March 1, 2025

In nature, plants and insects engage in intricate interactions. Despite the increasing knowledge of microbiomes insects, extent to which they exchange alter each other's remains unclear. this work, bacterial community associated with nymphs Philaenus spumarius (Hemiptera: Aphrophoridae), stems Coleostephus myconis where were feeding, foam produced by nymphs, studied culture-dependent -independent approaches, an attempt elucidate bacteria between insects. The results suggest that both approaches complement other, as many genera identified metabarcoding not detected culturing, vice versa. Overall, exhibited higher diversity than all samples showing enrichment known provide diverse benefits their host. Stems most similar composition, but Burkholderiaceae Moraxellaceae dominated stems, whereas Rhizobiaceae Sphingobacteriaceae foam. Nymphs exhibit distinct yet more found stem compared Indeed, enriched on endosymbiotic bacteria, mostly Candidatus Sulcia Sodalis, Nevertheless, during appeared several C. myconis, a significant number being incorporated into bacteriome nymph. Curvibacter, Cutibacterium, Methylobacterium, Pseudomonas Rhizobium are likely exchanged. also appear foam, notably species from Enhydrobacter, Pseudomonas, Roseomonas genera. More studies infer functions shared P. spumarius-C. needed.

Language: Английский

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