Carbapenemase-producing Klebsiella pneumoniae: molecular and genetic decoding

Trends in Microbiology, Journal Year: 2014, Volume and Issue: 22(12), P. 686 - 696

Published: Oct. 9, 2014

Language: Английский

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Type IV pili: dynamics, biophysics and functional consequences

Nature Reviews Microbiology, Journal Year: 2019, Volume and Issue: 17(7), P. 429 - 440

Published: April 15, 2019

Language: Английский

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Adhesins Involved in Attachment to Abiotic Surfaces by Gram-Negative Bacteria

Microbiology Spectrum, Journal Year: 2015, Volume and Issue: 3(4)

Published: July 2, 2015

ABSTRACT During the first step of biofilm formation, initial attachment is dictated by physicochemical and electrostatic interactions between surface bacterial envelope. Depending on nature these interactions, can be transient or permanent. To achieve irreversible attachment, cells have developed a series adhesins promoting specific nonspecific adhesion under various environmental conditions. This article reviews recent advances in our understanding secretion, assembly, regulation during with particular emphasis fimbrial, nonfimbrial, discrete polysaccharide Gram-negative bacteria.

Language: Английский

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Microbial nanowires for bioenergy applications

Current Opinion in Biotechnology, Journal Year: 2014, Volume and Issue: 27, P. 88 - 95

Published: Jan. 3, 2014

Language: Английский

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A comprehensive guide to pilus biogenesis in Gram-negative bacteria

Nature Reviews Microbiology, Journal Year: 2017, Volume and Issue: 15(6), P. 365 - 379

Published: May 12, 2017

Language: Английский

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Exceptionally widespread nanomachines composed of type IV pilins: the prokaryotic Swiss Army knives

FEMS Microbiology Reviews, Journal Year: 2014, Volume and Issue: 39(1), P. 134 - 154

Published: Nov. 26, 2014

Prokaryotes have engineered sophisticated surface nanomachines that allowed them to colonize Earth and thrive even in extreme environments. Filamentous machineries composed of type IV pilins, which are associated with an amazing array properties ranging from motility electric conductance, arguably the most widespread since distinctive proteins dedicated their biogenesis found known species prokaryotes. Several decades investigations, starting pili then a variety related systems both bacteria archaea, outlined common molecular structural bases for these nanomachines. Using as paradigm, we will highlight this review aspects key biological differences group filamentous structures.

Language: Английский

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Comparative genomics of freshwater Fe-oxidizing bacteria: implications for physiology, ecology, and systematics

Frontiers in Microbiology, Journal Year: 2013, Volume and Issue: 4

Published: Jan. 1, 2013

The two microaerophilic, Fe-oxidizing bacteria (FeOB) Sideroxydans ES-1 and Gallionella ES-2 have single circular chromosomes of 3.00 3.16 Mb that encode 3049 3006 genes, respectively. Multi-locus sequence analysis (MLSA) confirmed the relationship these organisms to one another, indicated they may form a novel order, Gallionellalaes, within Betaproteobacteria. Both are adapted for chemolithoautotropy, including pathways CO2-fixation, electron transport growth at low O2-levels, an important adaptation growing on Fe(II). genomes contain Mto-genes implicated in iron-oxidation, as well other genes could be involved Fe-oxidation. Nearly 10% their devoted environmental sensing, signal transduction, chemotaxis, consistent with requirement narrow redox gradients Fe(II) O2. There differences well. is more metabolically flexible, can utilize reduced S-compounds, thiosulfate, lithotrophic growth. It has suite nitrogen fixation. contains additional gene clusters exopolysaccharide production, capacity resist heavy metals. strains hemerythrins globins, but especially high numbers oxygen homeostasis, or storage. share homology marine FeOB Mariprofundus ferrooxydans PV-1 CO2 fixation respiratory genes. In addition, shares 20 potentially active PV-1, large prophage. Combined genetic, morphological, physiological indicate species, lithotrophicus ES-1(T) (ATCC 700298(T); JCM 14762; DSMZ 22444; NCMA B100), capsiferriformans ES-2(T) 700299(T); 14763; 22445; B101).

Language: Английский

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Type IV Pili in Gram-Positive Bacteria

Microbiology and Molecular Biology Reviews, Journal Year: 2013, Volume and Issue: 77(3), P. 323 - 341

Published: Sept. 1, 2013

SUMMARY Type IV pili (T4P) are surface-exposed fibers that mediate many functions in bacteria, including locomotion, adherence to host cells, DNA uptake (competence), and protein secretion can act as nanowires carrying electric current. T4P composed of a polymerized protein, pilin, their assembly apparatuses share homologs with type II systems eubacteria the flagella archaea. found throughout Gram-negative bacterial families have been studied most extensively certain model species. Recently, it was discovered also widespread among Gram-positive species, particular clostridia. Since bacteria differences cell wall architecture other features, is remarkable how similar core proteins between these organisms, yet there key interesting be well. In this review, we compare two identify discuss features they common where differ provide very broad-based view across all eubacterial

Language: Английский

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Epidemic Klebsiella pneumoniae ST258 Is a Hybrid Strain

mBio, Journal Year: 2014, Volume and Issue: 5(3)

Published: June 25, 2014

ABSTRACT Carbapenem-resistant Enterobacteriaceae (CRE), especially Klebsiella pneumoniae carbapenemase (KPC)-producing K. , pose an urgent threat in health facilities the United States and worldwide. isolates classified as sequence type 258 (ST258) by multilocus typing are largely responsible for global spread of KPC. A recent comparative genome study revealed that ST258 strains two distinct genetic clades; however, molecular origin remains unknown, our understanding evolution clades is incomplete. Here we compared structures single-nucleotide polymorphism (SNP) distributions core genomes from other STs (ST11, ST442, ST42). We identified ~1.1-Mbp region on homogeneous to while rest resembles ST11. Our results suggest a hybrid clone—80% originated ST11-like 20% ST442-like strains. Meanwhile, sequenced ST42 strain carries same K-antigen-encoding capsule polysaccharide biosynthesis gene ( cps ) clade I Comparison -harboring regions between (clades II) suggests evolved II result replacement. findings unravel history strains, important first step toward development diagnostic, therapeutic, vaccine strategies combat infections caused multidrug-resistant . IMPORTANCE Recombination events replacement chromosomal have been documented various bacteria, these given rise successful pathogenic clones. used genomic analyses discover hybrid—80% chromosome homologous ST11 remaining ST442. indicated can be segregated into clades, with regions. analysis through recombination region. Horizontal transfer appears key element driving diversification These not only extend but effective control treatment pneumoniae.

Language: Английский

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Mechanisms of DNA Uptake by Naturally Competent Bacteria

Annual Review of Genetics, Journal Year: 2019, Volume and Issue: 53(1), P. 217 - 237

Published: Aug. 21, 2019

Transformation is a widespread mechanism of horizontal gene transfer in bacteria. DNA uptake to the periplasmic compartment requires DNA-uptake pilus and DNA-binding protein ComEA. In gram-negative bacteria, first pulled toward outer membrane by retraction then taken up binding ComEA, acting as Brownian ratchet prevent backward diffusion. A similar probably operates gram-positive bacteria well, but these systems have been less well characterized. Transport, defined movement single strand transforming cytosol, channel ComEC. Although understood about this process, it may be driven proton symport. review we also describe various phenomena that are coordinated with expression competence for transformation, such fratricide, kin-discriminatory killing neighboring cells, competence-mediated growth arrest.

Language: Английский

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