The Combination of Intestinal Alkaline Phosphatase Treatment with Moderate Physical Activity Alleviates the Severity of Experimental Colitis in Obese Mice via Modulation of Gut Microbiota, Attenuation of Proinflammatory Cytokines, Oxidative Stress Biomarkers and DNA Oxidative Damage in Colonic Mucosa

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(6), P. 2964 - 2964

Published: March 9, 2022

Inflammatory bowel diseases (IBD) are commonly considered as Crohn's disease and ulcerative colitis, but the possibility that alterations in gut microbiota oxidative stress may affect course of experimental colitis obese physically exercising mice treated with intestinal alkaline phosphatase (IAP) has been little elucidated. Mice fed a high-fat-diet (HFD) or normal diet (ND) for 14 weeks were randomly assigned to exercise on spinning wheels (SW) 7 IAP followed by intrarectal administration TNBS. The activity index (DAI), grip muscle strength test, biomarkers (MDA, SOD, GSH), DNA damage (8-OHdG), plasma levels cytokines IL-2, IL-6, IL-10, IL-12p70, IL-17a, TNF-α, MCP-1 leptin assessed, stool composition was determined next generation sequencing (NGS). TNBS-induced worsened sedentary manifested severe colonic damage, an increase DAI, biomarkers, decreased strength. longer running distance weight loss observed given subjected + SW compared ones. Less heterogeneous microbial noticed this effect disappeared mice. Absence Alistipes, lower proportion Turicibacter, Proteobacteria Faecalibacterium, Firmicutes Clostridium, decrease 8-OHdG content proinflammatory supplementation combination moderate physical attenuates severity murine complicated obesity through mechanism involving downregulation cytokine/chemokine network stress, modulation improvement

Language: Английский

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Mammalian Neuraminidases in Immune-Mediated Diseases: Mucins and Beyond

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: April 11, 2022

Mammalian neuraminidases (NEUs), also known as sialidases, are enzymes that cleave off the terminal neuraminic, or sialic, acid resides from carbohydrate moieties of glycolipids and glycoproteins. A rapidly growing body literature indicates in addition to their metabolic functions, NEUs regulate activity glycoprotein targets. The simple post-translational modification NEU protein targets-removal highly electronegative sialic acid-affects folding, alters interactions with ligands, exposes covers proteolytic sites. Through such effects, downstream processes which targets participate. major target desialylation by mucins (MUCs), contributes regulation disease processes. In this review, we focus on regulatory roles NEU-modified MUCs coordinators pathogenesis fibrotic, inflammatory, infectious, autoimmune diseases. Special attention is placed most abundant best studied NEU1, its recently discovered important target, mucin-1 (MUC1). role NEU1 - MUC1 axis discussed, along contributions other pathophysiologically

Language: Английский

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Postmortem submersion interval estimation of cadavers recovered from freshwater based on gut microbial community succession

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Dec. 2, 2022

Microbial community succession during decomposition has been proven to be a useful tool for postmortem interval (PMI) estimation. Numerous studies have shown that the intestinal microbial presented chronological changes after death and was stable in terrestrial corpses with different causes of death. However, pattern cadavers retrieved from water remains unclear. For immersed corpses, submersion (PMSI) is indicator PMI. To provide reliable estimates PMSI forensic investigations, we investigated gut submersed freshwater explored its potential application investigation. In this study, mouse died drowning or CO2 asphyxia (i.e., submersion) were characterized by 16S rDNA amplification high-throughput sequencing, followed bioinformatic analyses. The results demonstrated bacterial communities not between groups. α-diversity decreased significantly within 14 days both groups, β-diversity structure ordinated chronologically, inferring functional pathway phenotype. estimate PMSI, regression model established random forest (RF) algorithm based on microbiota. Furthermore, 15 genera, including Proteus, Enterococcus, others, selected as candidate biomarkers set up concise predicted model, which provided prediction [MAE (± SE) = 0.818 0.165) d]. Overall, our present study provides evidence would valuable marker submerged an aquatic habitat.

Language: Английский

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Role of exercise in preventing and restoring gut dysbiosis in patients with inflammatory bowel diseases: A review

World Journal of Gastroenterology, Journal Year: 2021, Volume and Issue: 27(30), P. 5037 - 5046

Published: Aug. 10, 2021

Inflammatory bowel diseases (IBD) include a spectrum of chronic inflammatory disorders the gastrointestinal tract whose pathogenesis is yet to be elucidated. The intestinal microbiome has been studied as causal component, with certain microbiotic alterations having observed in subtypes IBD. Physical exercise modulator microbiome, causing shifts its composition that are partially corrective those IBD; furthermore, physical may beneficial patients IBD subtypes. This review studies effects on human gut while investigating pathophysiologic mechanisms could explain activity's clinical

Language: Английский

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Voluntary Wheel Running in Old C57BL/6 Mice Reduces Age-Related Inflammation in the Colon but Not in the Brain

Cells, Journal Year: 2022, Volume and Issue: 11(3), P. 566 - 566

Published: Feb. 6, 2022

Inflammation is considered a possible cause of cognitive decline during aging. This study investigates the influence physical activity and social isolation in old mice on their functions inflammation. The Barnes maze task was performed to assess spatial learning memory 3, 9, 15, 24, 28 months male C57BL/6 as well following voluntary wheel running (VWR) (SI) 20 mice. Inflammatory gene expression analyzed hippocampal colonic samples by qPCR. Cognitive occurs between 15 24 age. VWR improved while SI had negative effects. Expression inflammatory markers changed aging hippocampus (Il1a/Il6/S100b/Iba1/Adgre1/Cd68/Itgam) colon (Tnf/Il6/Il1ra/P2rx7). attenuates inflammaging specifically (Ifng/Il10/Ccl2/S100b/Iba1), regulates intestinal Il1b Gfap. were not altered SI. main finding our that both exhibit an increase aging, age exclusively Based existence gut-brain axis, results extend therapeutic approaches preserving elderly colon.

Language: Английский

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Comparing the Impact of Continuous Endurance and High-intensity Interval Training on Cecal Butyrate and Propionate Levels in Diabetic Rats Induced by High-fat Diet

Annals of Military and Health Sciences Research, Journal Year: 2024, Volume and Issue: 22(3)

Published: Sept. 15, 2024

Background: Dysbiosis and metabolic disorders of the microbiota, often caused by an imbalance in intestinal microbial composition, are significant issues linked to immobility, obesity, diabetes. Physical exercise is recognized for its role managing these symptoms regulating composition metabolites thereby improving gut health overall function. Objectives: This study aimed investigate compare effects continuous endurance training (CET) high-intensity interval (HIIT) on two key cecal microbiota metabolites, butyrate propionate, diabetic rats. Methods: Forty-five male Wistar rats were made a high-fat diet trained under CET HIIT protocols. Cecal tissue samples taken from evaluate effect exercise, levels measured using high-performance liquid chromatography (HPLC) method. Results: Among patterns studied, significantly improved concentrations while showed no metabolites. Conclusions: Our findings suggest that, unlike CET, may effectively mitigate disturbances resulting dysbiosis patients. However, any definitive conclusion about exercises necessitates further comprehensive tests other examination additional supporting evidence, such as changes microbiome.

Language: Английский

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