Current Microbiology, Journal Year: 2024, Volume and Issue: 82(1)
Published: Dec. 24, 2024
Language: Английский
Genes, Journal Year: 2024, Volume and Issue: 15(1), P. 64 - 64
Published: Jan. 1, 2024
Aquaculture plays a crucial role in meeting the increasing global demand for food and protein sources. However, its expansion is followed by challenges, such as infectious disease outbreaks antibiotic misuse. The present study focuses on genetic functional analyses of two
Language: Английский
Citations
4
Antibiot Khimioter = Antibiotics and Chemotherapy, Journal Year: 2025, Volume and Issue: 69(9-10), P. 17 - 23
Published: Feb. 8, 2025
Background. The precise identification of microorganism species that cause infectious complications in hospitalized patients is beyond doubt relevant modern healthcare. aim this study was to determine the possibility using Sanger sequencing routine microbiological examination an Internal Medicine Clinic improve quality etiological diagnosis bacterial complications. Material and methods. Clinical isolates microorganisms isolated from a multidisciplinary medical center were studied. used classical methods seeding cultures, as well sequencing. Results. MicroSeq system (Applied Biosystems, USA) ensured all 231 studied – causative agents nosocomial infections. For differential diagnostics streptococci coagulase-negative staphylococci, some cases, when known sequences first 500 nucleotides 16S rRNA gene two differed by 1–2 nucleotides, increasing discrimination level 100% allowed valid determination affiliation microorganism. Phenotypic failed identify significant proportion (25.9%) infection pathogens, only 8 (13.8%) them reliably identified cases. use bacteria led long-term effect associated with improved qualifications laboratory doctors, enhanced discriminatory capabilities visual assessment macromorphology which important for identifying types present biosubstrates. Conclusion. method highly efficient quite cost effective, compared biochemical test panels widely clinical practice — «gold standard» clinic internal diseases.
Language: Английский
Citations
0
Biotechnology and Applied Biochemistry, Journal Year: 2025, Volume and Issue: unknown
Published: March 14, 2025
ABSTRACT As an emerging technology, Raman spectroscopy (RS) has been used to identify pathogenic bacteria with excellent performance. The aim of this study was verify the diagnosis accuracy RS in identification bacteria. This meta‐analysis systematically evaluated for We searched electronic databases PubMed and Web Science obtain relevant articles; STATA 15.1 analyze all sensitivities, specificies, their 95% confidence interval (CI). summary receiver operating characteristic curves (SROC) area under curve (AUC) were display more performance RS. Nineteen articles included according inclusion exclusion criteria. pooled sensitivity specificity 0.94 (95% CI, 0.89–0.96) 0.99 0.97–0.99). diagnostic odds ratio (DOR) 1209 367–3980), AUC SROC 0.98–1.00). For gram‐positive bacteria, different species ranged from 0.00 1.00 0.96 1.00, a 0.90–0.98) gram‐negative 0.30 0.92 0.76–0.98) acid‐fast 0.83 0.84–0.99) 0.96–1.00). provides new method demonstrates high most species.
Language: Английский
Citations
0
Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)
Published: April 17, 2025
Intratumoral microbiota has been found to be a crucial component of hepatocellular carcinoma (HCC). Due insufficient recognition, technical limitations, and low biomass intratumoral microbiota, it is poorly understood. exhibit significant diversity in HCC tissues. It involved the development through several mechanisms, such as remodeling immunosuppressive microenvironment, metabolic reprogramming, genetic alterations. Moreover, associated with metastasis cells. Herein, we reviewed history applied biotechnology depict signatures investigated potential sources assessed their functions, heterogeneity. Furthermore, this review, summarized therapeutics that can used treatment proposed future perspectives for research field.
Language: Английский
Citations
0
Aquaculture International, Journal Year: 2025, Volume and Issue: 33(4)
Published: May 2, 2025
Language: Английский
Citations
0
Microorganisms, Journal Year: 2024, Volume and Issue: 12(4), P. 833 - 833
Published: April 20, 2024
Currently, there is a wide application in the literature of use Fourier Transform Infrared Spectroscopy (FTIR) technique. This basic tool has also proven to be efficient for detecting molecules associated with hosts and pathogens infections, as well other present humans animals’ biological samples. However, crisis science data reproducibility. can observed from experimental animal models (EAMs). When it comes rodents, major challenge carry out sanitary monitoring, which currently expensive requires large volume samples, generating ethical, legal, psychological conflicts professionals researchers. We carried survey relevant on this technique different diagnostic protocols combined aim presenting promising EAM. Since FTIR detect diseases advantages such low samples required, cost, sustainability, provides tests high specificity sensitivity, we believe that highly stress detection interest infectious or non-infectious origin.
Language: Английский
Citations
3
Diagnostics, Journal Year: 2024, Volume and Issue: 14(12), P. 1234 - 1234
Published: June 12, 2024
A total of 300 research participants—200 consecutive patients diagnosed with dyslipidemia (100 statin (+), treated for at least five years, and 100 (−)) healthy controls—were included in this observational study. The aim the study was to deliver insights into relationship between long-term use statins gallstone disease (GSD), as well background particularities gut microbiota. All participants underwent clinical examination, laboratory workups, stool microbiology/stool 16S r RNA, next-generation sequencing, abdominal ultrasound/CT exams. Results: presented similarities related age, gender, location. Patients displayed comparable heredity GSs, metabolic issues, co-morbidities. Gut dysbiosis (DB) present 54% (−) vs. 35% (+) (p = 0.0070). GSs were 14% group 5% 0.0304). Severe dysbiosis, a significant reduction biodiversity, an increase LPS bacteria, notable decrease mucin-degrading mucosa-protective butyrate-producing bacteria observed group. Strong positive correlations GSD diabetes/impaired glucose tolerance (r 0.3368, p 0.0006), obesity 0.3923, < 0.0001), nonalcoholic fatty liver 0.3219, 0.0011), DB 0.7343, negative alcohol −0.2305, 0.0211), observed. multiple regression equation demonstrated that only (95% CI: 0.3163 0.5670; 0.0001) 0.01431 0.2578; 0.0289) independent risk factors predicting statins. Conclusion: dyslipidemic associated low developing GSs. microbiota characterized by imbalance various functional alterations found be patients.
Language: Английский
Citations
3
BMC Infectious Diseases, Journal Year: 2024, Volume and Issue: 24(1)
Published: Oct. 24, 2024
Micrococcus antarcticus (M. antarcticus) is an aerobic Gram-positive spherical actinobacterium that was initially isolated from Chinese Great-Wall station in Antarctica 2000. M. considered to be of low pathogenicity, no previous cases human infection by this organism have been reported. Here we describe the first report with community-acquired pneumonia (CAP) caused antarcticus. An 87-year-old female presented Central Hospital Wuhan November 2023 a chief complaint cough, sputum production, and chest tightness for 2 weeks. Microbial culture patient's bronchoalveolar lavage fluid (BALF) identification isolates using Matrix-assisted laser desorption ionization/time flight mass spectrometry (MALDI-TOF MS) 16S rRNA gene sequencing revealed infection. Combined clinical symptoms, laboratory imaging examination, patient diagnosed CAP. Then cefoperazone/sulbactam levofloxacin administrated, condition improved she discharged after week admission, abnormalities were detected during 5-month follow-up. This case highlights antarcticus, identified CAP, extremely rare pathogenic microorganism. Clinicians should aware its potential as pathogen diagnosis treatment
Language: Английский
Citations
3
Animal Diseases, Journal Year: 2024, Volume and Issue: 4(1)
Published: Oct. 1, 2024
Abstract The gastrointestinal tract of felines is inhabited by an active and intricate population microorganisms whose alteration creates disturbances in the immune response can affect health disease states. Studies using various analytical methods have identified peculiar trends illnesses, with Firmicutes being most prevalent phylum, followed Bacteroidetes, Proteobacteria, Actinobacteria. However, more fewer Bacteroidetes been observed cats infected Feline coronavirus. Alterations composition these gut microbiota be solved modification through dietary fiber, probiotics, fecal transplantation. Therefore, it critical to understand microbiota, changes roles environment, importance concepts for overall while considering exchange microbes between humans domestic animals. This review provides comprehensive information on feline composition, modulation, analytic used characterizing microbiota.
Language: Английский
Citations
0
Current Microbiology, Journal Year: 2024, Volume and Issue: 82(1)
Published: Dec. 24, 2024
Language: Английский
Citations
0