Effect of Extracelluar Vesicles Derived from Akkermansia muciniphila on Intestinal Barrier in Colitis Mice

Nutrients, Journal Year: 2023, Volume and Issue: 15(22), P. 4722 - 4722

Published: Nov. 8, 2023

Inflammatory bowel disease (IBD) is a chronic and recurrent disease. It has been observed that the incidence prevalence of IBD are increasing, which consequently raises risk developing colon cancer. Recently, regulation intestinal barrier by probiotics become an effective treatment for colitis. Akkermansia muciniphila-derived extracellular vesicles (Akk EVs) nano-vesicles contain multiple bioactive macromolecules with potential to modulate barrier. In this study, we used ultrafiltration in conjunction high-speed centrifugation extract Akk EVs. A lipopolysaccharide (LPS)-induced RAW264.7 cell model was established assess anti-inflammatory effects found EVs were able be absorbed cells significantly reduce expression nitric oxide (NO), TNF-α, IL-1β (p < 0.05). We explored preventative on colitis regulating using mouse caused dextran sulfate sodium (DSS). The findings demonstrated effectively prevented symptoms reduced colonic tissue injury. Additionally, enhanced effectiveness elevating MUC2 (0.53 ± 0.07), improving mucus integrity, reducing permeability Moreover, increased proportion beneficial bacteria Firmicutes (33.01 0.09%) downregulated harmful Proteobacteria (0.32 0.27%). These suggest possess ability regulate immune responses, protect barriers, gut microbiota. research presents intervention approach prevent

Language: Английский

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Chlorogenic Acid Ameliorates Post‐Infectious Irritable Bowel Syndrome by Regulating Extracellular Vesicles of Gut Microbes

Advanced Science, Journal Year: 2023, Volume and Issue: 10(28)

Published: Aug. 24, 2023

Post-infectious irritable bowel syndrome (PI-IBS) occurs after acute infectious diarrhea, and dysbiosis can be involved in its pathogenesis. Here, the role of chlorogenic acid (CGA) is investigated, a natural compound with several pharmacological properties, alleviating PI-IBS rats. It elucidated that gut microbiota plays key pathogenesis rectal administration CGA alleviated by modulating metabolites. supplementation significantly increased fecal Bacteroides acidifaciens abundance glycine levels. Glycine structurally altered B. extracellular vesicles (EVs) enriched functional proteins EVs; glycine-induced EVs reducing inflammation hypersensitivity intestinal viscera maintaining mucosal barrier function. Moreover, are brain tissue. Thus, mediates mitigation through This study proposes novel mechanism signal exchange between microenvironment host.

Language: Английский

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Human umbilical cord mesenchymal stem cells ameliorate colon inflammation via modulation of gut microbiota-SCFAs-immune axis

Stem Cell Research & Therapy, Journal Year: 2023, Volume and Issue: 14(1)

Published: Sept. 25, 2023

Abstract Background Inflammatory bowel disease (IBD) is a global health problem in which gut microbiota dysbiosis plays pivotal pathogenic role. Mesenchymal stem cells (MSCs) therapy has emerged as prospective novel tool for managing IBD, and can also regulate the composition of microbiota. However, functional significance MSCs-induced changes microbiome poorly understood. Methods Here, we investigated first time role mediating protective effect human umbilical cord MSCs (HUMSCs) on DSS-induced colitis. Gut alteration short-chain fatty acids (SCFAs) production were analyzed through 16S rRNA sequencing targeted metabolomics. Spectrum antibiotic cocktail (ABX), fecal transplantation (FMT) sterile filtrate (SFF) employed to evaluate intestinal flora its metabolites. Cytokine microarray, Enzyme-linked immunosorbent assay (ELISA), flow cytometry conducted assess CD4 + T homeostasis. Results By performing depletion experiments, revealed that derived from ameliorated colon inflammation reshaped T-cells immune homeostasis via remodeling diversity flora, especially up-regulated SCFAs-producing bacterial abundance, such Akkermansia , Faecalibaculum Clostridia_UCG_014 . Consistently, metabolomics manifested increased SCFAs with administration, there was significant positive correlation between differential bacteria SCFAs. Meanwhile, combined gavage underlying mechanism further associated improved Treg/Th2/Th17 balance mucosa mediated microbiota-derived production. Conclusion The present study advances understanding effects colitis, providing evidence new microbiome-metabolite-immune axis recovery colitis by MSCs.

Language: Английский

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Health and Disease: Akkermansia muciniphila , the Shining Star of the Gut Flora

Research, Journal Year: 2023, Volume and Issue: 6

Published: Jan. 1, 2023

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Bio-inspired glycosylated nano-hydroxyapatites enhance endogenous bone regeneration by modulating macrophage M2 polarization

Acta Biomaterialia, Journal Year: 2023, Volume and Issue: 162, P. 135 - 148

Published: March 25, 2023

Language: Английский

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