Regenerative Biomaterials,
Journal Year:
2024,
Volume and Issue:
11
Published: Jan. 1, 2024
Abstract
Currently,
the
successful
healing
of
critical-sized
calvarial
bone
defects
remains
a
considerable
challenge.
The
immune
response
plays
key
role
in
regulating
regeneration
after
material
grafting.
Previous
studies
mainly
focused
on
relationship
between
macrophages
and
marrow
mesenchymal
stem
cells
(BMSCs),
while
dural
were
recently
found
to
play
vital
healing.
In
this
study,
series
3D
elastomers
with
different
proportions
polycaprolactone
(PCL)
poly(glycerol
sebacate)
(PGS)
fabricated,
which
further
supplemented
polydopamine
(PDA)
coating.
physicochemical
properties
PCL/PGS
PCL/PGS/PDA
grafts
measured,
then
they
implanted
as
filling
materials
for
8
mm
defects.
results
showed
that
matched
effective
PDA
interface
formed
well-proportioned
elastomer,
effectively
modulated
polarization
M2
promoted
recruitment
achieve
full-thickness
repair
through
both
intramembranous
endochondral
ossification.
Single-cell
RNA
sequencing
analysis
revealed
predominance
during
their
close
macrophages.
findings
illustrated
crosstalk
determined
vertical
first
time,
may
be
new
target
designing
Chemical Engineering Journal,
Journal Year:
2024,
Volume and Issue:
488, P. 150631 - 150631
Published: March 26, 2024
The
skeletal
system
is
essential
for
preserving
body
structure
and
facilitating
mobility;
however,
bone
diseases
frequently
result
in
significant
disabilities,
necessitating
the
investigation
into
biomaterials
restoration.
Nevertheless,
these
may
elicit
immune
reactions
that
obstruct
healing
process.
Macrophages
assume
a
pivotal
role
modulating
inflammation
sustaining
homeostasis
regeneration.
A
comprehensive
understanding
of
molecular
interactions
between
macrophages
imperative
development
sophisticated
designed
to
regulate
environment
promote
remodeling.
This
review
delves
generation,
polarization,
interaction
with
principal
cell
types
implicated
osteogenesis,
detailing
mechanisms
by
which
influence
repair
their
response
biomaterials.
Additionally,
it
discusses
strategies
precise
modulation
macrophage
functionality
via
intelligent
mechanisms,
outlines
challenges
constraints
designing
integrate
functions.
By
elucidating
challenges,
lays
groundwork
creation
advanced
endowed
smart
immunomodulatory
capabilities,
heralding
novel
approaches
treatment
defects
advancement
regeneration
therapies.
Biomacromolecules,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 28, 2025
Wound
healing
remains
a
global
challenge
for
clinical
and
experimental
research.
Hydrogels
prepared
from
natural
polysaccharides
show
great
potential
in
the
wound
process.
In
this
study,
novel
hydrogels
(G-GLP)
were
using
oxidized
Ganoderma
lucidum
(OGLPs)
carboxymethyl
chitosan
via
Schiff
base
reaction,
which
did
not
require
addition
of
any
chemical
cross-linking
agent.
The
showed
excellent
mechanical
properties
biocompatibility.
Moreover,
superior
hemostatic
performance
mouse
liver
trauma
tail
amputation
models.
Importantly,
G-GLP
improved
inflammation
by
promoting
polarization
macrophage
M2
subtype,
inhibiting
M1
subtype
reducing
intracellular
levels
reactive
oxygen
species.
vivo
experiments
demonstrated
that
accelerated
total
defect
model
blood
vessel
repair
collagen
deposition.
These
results
demonstrate
has
as
an
effective
dressing.
Military Medical Research,
Journal Year:
2024,
Volume and Issue:
11(1)
Published: Oct. 21, 2024
Abstract
Severe
tissue
defects
present
formidable
challenges
to
human
health,
persisting
as
major
contributors
mortality
rates.
The
complex
pathological
microenvironment,
particularly
the
disrupted
immune
landscape
within
these
defects,
poses
substantial
hurdles
existing
regeneration
strategies.
However,
emergence
of
nanobiotechnology
has
opened
a
new
direction
in
immunomodulatory
nanomedicine,
providing
encouraging
prospects
for
and
restoration.
This
review
aims
gather
recent
advances
nanomedicine
foster
regeneration.
We
begin
by
elucidating
distinctive
features
local
microenvironment
defective
tissues
its
crucial
role
Subsequently,
we
explore
design
functional
properties
nanosystems.
Finally,
address
clinical
translation
development,
aiming
propose
potent
approach
enhance
through
synergistic
modulation
integration.
ACS Applied Materials & Interfaces,
Journal Year:
2024,
Volume and Issue:
16(7), P. 8442 - 8458
Published: Feb. 9, 2024
The
disruption
of
host–microbe
homeostasis
and
uncontrolled
inflammatory
response
have
been
considered
as
vital
causes
for
developing
periodontitis,
subsequently
leading
to
an
imbalance
between
the
bone
immune
system
collapse
homeostasis.
Consequently,
strategies
modulate
metabolization
become
a
promising
approach
prevent
treat
periodontitis.
In
this
study,
we
investigated
cooperative
effects
Nel-like
molecule
type
1
(Nell-1)
gold
nanoparticles
(AuNPs)
on
macrophage
polarization,
osteoclast
differentiation,
corresponding
functions
in
experimental
model
periodontitis
rats.
Nell-1-combined
AuNPs
vitro
studies
were
found
reduce
production
factors
(TNF-α,
p
<
0.0001;
IL-6,
=
0.0012),
ratio
M2/M1
macrophages
by
inducing
polarization
into
M2
phenotype,
inhibit
cell
fusion,
maturation,
activity
osteoclasts.
Furthermore,
local
application
vivo
resulted
alleviation
damages
periodontal
tissues,
modulation
osteoclasts,
alteration
microbiota,
which
relative
abundance
probiotic
Bifidobacterium
increased
(p
0.05).
These
findings
reveal
that
could
be
drug
candidate
prevention
treatment
However,
did
not
show
organ
toxicity
or
impair
integrity
intestinal
epithelium
but
alter
gut
dysbiosis
microbiota.
adverse
impact
changes
microbiota
needs
further
investigated.
Nonetheless,
study
provides
novel
perspective
direction
biological
safety
assessment
biomaterials
oral
clinical
applications.
Materials & Design,
Journal Year:
2024,
Volume and Issue:
241, P. 112903 - 112903
Published: March 31, 2024
The
development
of
osteoimmunology
has
highlighted
the
crucial
role
biomaterial
surface
topography
in
modulating
macrophage
responses,
subsequently
affecting
bone
formation.
However,
underlying
mechanism
remains
largely
unexplored.
This
study
introduced
two
biphasic
calcium
phosphate
(BCP)
ceramics
with
different
architecture,
finding
that
compared
to
micron-scale
counterparts
(BCP2,
∼3.07
μm),
BCP
submicron-scale
structure
(BCP1,
∼0.66
μm)
enhanced
ectopic
formation
and
facilitated
M2
polarization
vivo.
Previous
studies
have
focused
predominantly
on
soluble
cytokines
secreted
by
macrophages,
significance
exosomes
intercellular
communication
been
overlooked.
isolated
from
macrophages
treated
either
submicron-
or
surfaces,
designated
as
B1-Exos
B2-Exos,
respectively.
Notably,
could
significantly
improve
osteogenic
differentiation
mesenchymal
stem
cells
(MSCs),
potentially
attributable
miRNA
cargos
within
these
exosomes.
SmallRNA
sequencing,
western
blotting,
qRT-PCR,
bioinformatics
analyses
indicated
submicron-sized
BCP1
ceramic
markedly
elevated
miR-142a-5p
levels
macrophage-derived
exosomes,
activating
PTEN/AKT
signaling
pathway,
consequently
guiding
MSCs
towards
osteoblast
lineage.
These
findings
not
only
deepen
our
understanding
biomaterial-mediated
osteoinduction
mechanism,
but
also
inform
design
repair
materials
specialized
for
proper
immunomodulation
initiate
osteogenesis.
Journal Of Clinical Periodontology,
Journal Year:
2024,
Volume and Issue:
51(8), P. 1054 - 1065
Published: May 12, 2024
Abstract
Aim
To
investigate
the
association
between
periodontal
macrophage
polarization
states
and
alveolar
bone
levels,
to
assess
whether
glycosylated
nano‐hydroxyapatites
(GHANPs)
could
improve
regeneration
in
periodontitis
by
inducing
M2
polarization.
Materials
Methods
The
change
of
state
inflammatory
tissues
(with
loss)
was
examined
using
clinical
gingival
samples.
relationship
phenotype
level
loss
repair
evaluated
a
mouse
model.
effect
GHANPs
on
assessed
vitro
model
lipopolysaccharide
(LPS)‐stimulated
inflammation.
polarization‐related
markers
were
detected
immunofluorescence
staining,
real‐time
polymerase
chain
reaction
enzyme‐linked
immunosorbent
assay
analysis.
therapeutic
explored
experimental
histological
staining
micro‐CT
Results
A
lower
M2/M1
ratio
observed
periodontitis‐affected
human
tissues.
results
animal
experiments
demonstrated
positive
correlation
Arg‐1/iNOS
accelerated
loss;
also,
proportion
Arg‐1‐positive
macrophages
increased
during
regeneration.
administration
partially
restored
after
LPS
stimulation.
induced
ligation,
potentially
related
their
transition
regulation.
Conclusions
findings
this
study
indicate
that
induction
can
be
considered
viable
approach
for
enhancing
repair.
Additionally,
show
potential
treatment
periodontitis.
