Emergence and Dissemination of Extraintestinal Pathogenic High-Risk International Clones of Escherichia coli

Life, Journal Year: 2022, Volume and Issue: 12(12), P. 2077 - 2077

Published: Dec. 10, 2022

Multiresistant Escherichia coli has been disseminated worldwide, and it is one of the major causative agents nosocomial infections. E. a remarkable complex genomic plasticity for taking up accumulating genetic elements; thus, multiresistant high-risk clones can evolve. In this review, we summarise all available data about internationally extraintestinal pathogenic based on whole-genome sequence (WGS) confirmed outbreaks. Based markers, clustered into eight phylogenetic groups. Nowadays, ST131 clone from group B2 predominant worldwide. Currently, strains C1-M27 subclade within clade C are circulating becoming prominent in Canada, China, Germany, Hungary Japan. The characterised by blaCTX-M-27. Recently, ST1193 reported as an emerging B2. ST38 carrying blaOXA-244 (a blaOXA-48-like carbapenemase gene) caused several outbreaks Germany Switzerland. Further international include ST10, ST69, ST73, ST405, ST410, ST457. High-risk present different niches, human intestinal tract animals, persist environment. These be transmitted easily community well hospital settings. WGS analysis useful tool tracking dissemination resistance determinants, emergence mulitresistant to analyse changes population level.

Language: Английский

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Mobile genetic elements drive the multidrug resistance and spread of Salmonella serotypes along a poultry meat production line

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: March 16, 2023

The presence of mobile genetic elements in Salmonella isolated from a chicken farm constitutes potential risk for the appearance emerging bacteria present food industry. These contribute to increased pathogenicity and antimicrobial resistance through genes that are related formation biofilms contained plasmids, integrons, transposons. One hundred thirty-three isolates different stages production line, such as feed manufacturing, hatchery, broiler farm, poultry slaughterhouse, were identified, serotyped sequenced. most predominant serotype was Infantis. Phylogenetic analyses demonstrated diversity spread strains pipeline serotype-independent, belonging same very closely genetically. On other hand, Infantis carried pESI IncFIB plasmid harboring wide variety genes, all linked elements, among carriers these antibiograms showed differences profiles this structure, similarly observed Heidelberg carrying IncI1-Iα plasmid. Mobile encoding virulence also contributed gene content. Antibiotic genotypes matched by phenotypes, with high frequency tetracycline, aminoglycosides, cephalosporins resistance. In conclusion, contamination industry is described throughout entire leading multi-drug resistant bacteria, thus promoting survival when challenged various compounds.

Language: Английский

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Genomic features and comparative analysis of a multidrug-resistant Acinetobacter bereziniae strain infecting an animal: a novel emerging one health pathogen?

World Journal of Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 40(2)

Published: Jan. 8, 2024

Language: Английский

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Multidrug-Resistant High-Risk Escherichia coli and Klebsiella pneumoniae Clonal Lineages Occur in Black-Headed Gulls from Two Conservation Islands in Germany

Antibiotics, Journal Year: 2022, Volume and Issue: 11(10), P. 1357 - 1357

Published: Oct. 5, 2022

Multidrug-resistant (MDR) Enterobacterales, including extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae, not only emerge in healthcare settings but also other habitats, such as livestock wildlife. The spread of these pathogens, which often combine resistance with high-level virulence, is a growing problem, infections have become increasingly difficult to treat. Here, we investigated the occurrence ESBL-producing E. K. pneumoniae fecal samples from two black-headed gull colonies breeding on nature conservation islands Western Pomerania, Germany. In addition cloacal adult birds (n = 211) their nestlings 99) during 2021 season, collective 29) were obtained. All screened for ESBL producers, then subjected whole-genome sequencing. We found total 12 consisting 11 1 international high-risk sequence types (ST)131, ST38, ST58. Eight strains had MDR genotype carried large repertoire virulence-associated genes, pap operon, important urinary tract infections. addition, identified many genes associated adherence, biofilm formation, iron uptake, toxin production. Finally, our analysis revealed close phylogenetic relationship ST38 genomes originating human sources, underlining zoonotic pathogenic character. This study highlights importance One Health approach, thus interdependence between animal health surrounding environment.

Language: Английский

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Hydrocinnamic Acid and Perillyl Alcohol Potentiate the Action of Antibiotics against Escherichia coli

Antibiotics, Journal Year: 2023, Volume and Issue: 12(2), P. 360 - 360

Published: Feb. 9, 2023

The treatment of bacterial infections has been troubled by the increased resistance to antibiotics, instigating search for new antimicrobial therapies. Phytochemicals have demonstrated broad-spectrum and effective antibacterial effects as well antibiotic resistance-modifying activity. In this study, perillyl alcohol hydrocinnamic acid were characterized their action against Escherichia coli. Furthermore, dual triple combinations these molecules with antibiotics chloramphenicol amoxicillin investigated first time. Perillyl had a minimum inhibitory concentration (MIC) 256 µg/mL bactericidal (MBC) 512 µg/mL. Hydrocinnamic MIC 2048 an MBC > Checkerboard time-kill assays synergism or additive chloramphenicol/perillyl alcohol, chloramphenicol/hydrocinnamic acid, amoxicillin/hydrocinnamic at low concentrations both molecules. Combenefit analysis showed various each phytochemical. Combinations revealed mainly (up 2 μg/mL alcohol; 0.5 acid). results highlight potential combinatorial therapies microbial growth control, where phytochemicals can play important role potentiators agents.

Language: Английский

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The detection of KPC-2, NDM-1, and VIM-2 carbapenemases in international clones isolated from fresh vegetables highlights an emerging food safety issue

International Journal of Food Microbiology, Journal Year: 2024, Volume and Issue: 420, P. 110765 - 110765

Published: May 23, 2024

Language: Английский

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Emergence of Carbapenem-Resistant Uropathogenic Escherichia coli (ST405 and ST167) Strains Carrying blaCTX-M-15, blaNDM-5 and Diverse Virulence Factors in Hospitalized Patients

Pathogens, Journal Year: 2024, Volume and Issue: 13(11), P. 964 - 964

Published: Nov. 5, 2024

Urinary tract infections (UTIs) are common infectious diseases in hospital settings, and they frequently caused by uropathogenic

Language: Английский

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Evaluation of the resistome and gut microbiome composition of hospitalized patients in a health unit of southern Brazil coming from a high animal husbandry production region

Frontiers in Antibiotics, Journal Year: 2025, Volume and Issue: 3

Published: Jan. 17, 2025

Antimicrobial resistance (AMR) poses a significant threat to global public health. The One Health approach, which integrates human, animal, and environmental health, highlights the roles of agricultural hospital settings in propagation AMR. This study aimed analyze resistome gut microbiome composition individuals from high-intensity animal husbandry area western region Santa Catarina, Southern Brazil, who were subsequently admitted University Hospital city Florianopolis, located eastern part same state. Rectal swab samples collected upon admission discharge. Metagenomic sequencing analysis employed identify antimicrobial genes (ARGs) their associated bacterial taxa. Additionally, impact environment on profiles these patients was assessed. A total 247 genetic elements related AMR identified, with 66.4% present both discharge samples. Aminoglycoside most prevalent, followed by for tetracyclines lincosamides. Notably, unique genes, including dfrF mutations gyrB, identified at ARGs 55 species, Lactobacillus fermentum, harboring ermB gene. (MLSB), detected prevalent families included Mycobacteriaceae, Enterobacteriaceae, Bacteroidaceae. Among these, Mycobacteriaceae abundant, primarily 16S rRNA gene, RNA polymerase subunits, gyrases. revealed high prevalence aminoglycoside tetracycline resistance, notable increase certain determinants discharge, likely influenced extended use. presence mcr colistin single patient concerning trend AMR, particularly relation husbandry. These findings underscore substantial use development complex dynamics settings. They also emphasize influence local factors, such as intensive production, patterns advocate ongoing surveillance policy manage multidrug-resistant bacteria eVectively.

Language: Английский

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Whole genome sequencing and phylogenetic analysis to examine acquisition of Escherichia coli clones during international travel

Diagnostic Microbiology and Infectious Disease, Journal Year: 2025, Volume and Issue: 111(3), P. 116701 - 116701

Published: Jan. 18, 2025

Language: Английский

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Occurrence of virulence genes in multidrug-resistant Escherichia coli isolates from humans, animals, and the environment: One health perspective

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0317874 - e0317874

Published: Jan. 24, 2025

Escherichia coli is one of the critical One Health pathogens due to its vast array virulence and antimicrobial resistance genes. This study used multiplex PCR determine occurrence genes bfp , ompA traT eaeA stx1 among 50 multidrug-resistant (MDR) E . isolates from humans (n = 15), animals 29), environment 6) in Dar es Salaam, Tanzania. Their association with antimicrobial-resistant (ARGs) was determined using Principal Component Analysis (PCA). All 50/50 (100%) MDR carried at least gene, 19/50 (38%) carrying four genes, + The findings showed a high (82%), (78%), (72%); detected no any isolates. In humans, most were 14/15 (93.3%); for poultry, it 13/14 (92.9%); pigs, 13/15 (86.7%); while river water, 6/6 (100%). observed significant between ARGs. PCA results show contributed isolates, blaTEM blaCTX-M qnrs ellipses that pigs had more than those isolated humans. frequency numerous animals, indicates these have very potential cause diseases are difficult treat because they MDR.

Language: Английский

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