One Health Spread of 16S Ribosomal RNA Methyltransferase-Harboring Gram-Negative Bacterial Genomes: An Overview of the Americas

Pathogens, Journal Year: 2023, Volume and Issue: 12(9), P. 1164 - 1164

Published: Sept. 15, 2023

Aminoglycoside antimicrobials remain valuable therapeutic options, but their effectiveness has been threatened by the production of bacterial 16S ribosomal RNA methyltransferases (16S-RMTases). In this study, we evaluated genomic epidemiology 16S-RMTase genes among Gram-negative bacteria circulating in American continent. A total 4877 sequences were identified mainly Enterobacterales and nonfermenting bacilli isolated from humans, animals, foods, environment during 1931-2023. Most found United States, Brazil, Canada, Mexico, prevalence have increased last five years (2018-2022). The three species most frequently carrying Acinetobacter baummannii, Klebsiella pneumoniae, Escherichia coli. armA gene was prevalent, other (e.g., rmtB, rmtE, rmtF) could be emerging backstage. More than 90% Americas North countries, although less prevalent Central South these findings may underestimations due to limited data. Therefore, whole-genome sequence-based studies focusing on aminoglycoside resistance using a One Health approach low- middle-income countries should encouraged.

Language: Английский

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Rabbits as a Reservoir of Multidrug-Resistant Escherichia coli: Clonal Lineages and Public Health Impact

Antibiotics, Journal Year: 2024, Volume and Issue: 13(4), P. 376 - 376

Published: April 20, 2024

Escherichia coli, including extended-spectrum β-lactamases (ESBL)-producing strains, poses a global health threat due to multidrug resistance, compromising food safety and environmental integrity. In industrial settings, rabbits raised for meat have the highest consumption of antimicrobial agents compared other food-producing animals. The European Union is facing challenges in rabbit farming as declines antibiotic-resistant strains E. coli cause enteric diseases. aim this study was investigate antibiotic resistance profile, genetic diversity, biofilm formation cefotaxime-resistant isolated from twenty farms Northern Portugal address effect pressing issue industry. Resistance critically antibiotics observed, with high levels several categories, such tetracycline, ampicillin, aztreonam, streptomycin. However, all isolates were susceptible cefoxitin imipenem. Multidrug common, showing tested. blaCTX-M variants (blaCTX-3G blaCTX-M9), followed by tetracycline genes, most frequent genes found. ST10 clones exhibiting significant various categories harboring different detected. ST457 ST2325 important sequence types their association ESBL-E. been widely distributed variety environments host species. evaluated showed capacity formation, which varied when they grouped number classes (i.e., seven antibiotics, six three/four/five antibiotics). One Health approach integrates efforts combat through interdisciplinary collaboration human, animal, health. Our findings are worrisome raise concerns. extensive usage emphasizes urgent need establish active surveillance systems.

Language: Английский

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Genomic analysis of multidrug-resistant Escherichia coli strains carrying the mcr-1 gene recovered from pigs in Lima-Peru

Comparative Immunology Microbiology and Infectious Diseases, Journal Year: 2023, Volume and Issue: 99, P. 102019 - 102019

Published: July 7, 2023

Language: Английский

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One health clones of multidrug-resistant Escherichia coli carried by synanthropic animals in Brazil

One Health, Journal Year: 2022, Volume and Issue: 16, P. 100476 - 100476

Published: Dec. 21, 2022

WHO priority pathogens have disseminated beyond hospital settings and are now being detected in urban wild animals worldwide. In this regard, synanthropic such as pigeons (Columba livia) rodents (Rattus rattus, Rattus norvegicus Mus musculus) of interest to public health due their role reservoirs that can cause severe diseases. These usually live highly contaminated environments frequent interactions with humans, domestic animals, food chain, becoming sentinels anthropogenic activities. study, we report genomic data Escherichia coli strains selected for ceftriaxone ciprofloxacin resistance, isolated from black rats. Genomic analysis revealed the occurrence international clones belonging ST10, ST155, ST224 ST457, carrying a broad resistome beta-lactams, aminoglycosides, trimethoprim/sulfamethoxazole, fluoroquinolones, tetracyclines and/or phenicols. SNP-based phylogenomic investigation confirmed clonal relatedness high-risk lineages circulating at human-animal-environmental interface globally. Our results confirm dissemination CTX-M-positive E. Brazil, highlighting potential these infection sources hotspot clinically relevant resistance genes, which is critical issue within One Health perspective.

Language: Английский

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Antimicrobial resistance at the human–animal–environment interface: A focus on antimicrobial-resistant Escherichia coli transmission dynamics, clinical implications, and future directions

International Journal of One Health, Journal Year: 2024, Volume and Issue: unknown, P. 161 - 171

Published: July 1, 2024

Understanding and combating antimicrobial resistance (AMR) is increasingly centered on the intricate relationship between humans, animals, environment, with Escherichia coli being main source of AMR-related fatalities worldwide. E. coli, though prevalent in intestines humans warm-blooded creatures, demonstrates wide ecological adaptability both intestinal extraintestinal habitats. This study explores dynamics, implications, future directions antimicrobial-resistant (AREC) transmission clinical significance. We investigated spread antibiotic-resistant strains among illuminating impact healthcare agriculture practices, as well environmental contamination. The implications AREC infections are addressed, emphasizing challenges treatment due to limited antibiotic options, increased morbidity mortality rates, economic burdens systems agriculture, urgent need for a One Health approach combat AMR through collaborative efforts across disciplines. Proposed encompass enhanced surveillance, innovative stewardship, alternative modalities. Keywords: Health, surveillance strategies, dynamics.

Language: Английский

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