Proanthocyanidin alleviates testicular torsion/detorsion-induced ischemia/reperfusion injury in rats

Tissue and Cell, Journal Year: 2024, Volume and Issue: 89, P. 102459 - 102459

Published: July 9, 2024

Language: Английский

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Prophylactic Effects of Rhamnetin Flavonoid on Indomethacin‐Induced Gastric Ulceration by Modulating HSP 70/Bax, SOD/MDA and TNF‐α/IL‐10

Clinical and Experimental Pharmacology and Physiology, Journal Year: 2025, Volume and Issue: 52(4)

Published: Feb. 19, 2025

ABSTRACT Rhamnetin is a naturally occurring flavonoid compound found in many wild plant species and indigenous fruits. Despite its numerous biological potentials, such as anti‐inflammatory, antioxidant antimicrobial effects, there lack of literature elucidating gastroprotective action anticipating molecular mechanism. Natural products can be good alternative to overcome the side effects relapses associated with anti‐ulcer drugs. This study aims elucidate rhamnetin's acute toxicity using indomethacin ulceration model. Animals were arbitrarily divided into five groups: negative control group (A) positive (B), both treated 1% carboxymethyl cellulose; reference (C) receiving 20 mg/kg omeprazole; low‐dose (D) high‐dose (E) rhamnetin groups 30 60 mg/kg, respectively. After 1 h, rats Groups B–E subjected indomethacin‐induced ulceration. Toxicity evaluations indicated safety at doses up 400 rats, without any noticeable physiological alterations. (30 mg/kg) administered orally h before gastric ulcer ameliorated stomach lesions lowered index area by 73.81% 77.87%, supplementation histopathological alterations restored barriers, including pH mucin secretion. Moreover, rhamnetin‐treated exhibited increased anti‐apoptotic heat shock protein 70 decreased Bax tissues. These findings line accumulated MDA, superoxide dismutase, catalase prostaglandin E2 levels, reduced serum inflammatory mediators (TNF‐α interleukin‐6) elevated interleukin‐10 cytokines. The outcomes indicate cicatrising against indomethacin‐mediated ulceration, possibly due modulatory actions on oxidative stress, inflammation apoptotic pathways.

Language: Английский

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Investigation of the Presence of Papillomavirus in Bovine Testicles and Determination of γH2AX and Cytochrome C in Testicular Tissues Determined to be Infected

Veterinary Medicine and Science, Journal Year: 2025, Volume and Issue: 11(1)

Published: Jan. 1, 2025

ABSTRACT This study aimed to detect the presence of bovine papillomavirus (BPV) in testicular tissue bulls over 1‐year old by immunohistochemical, immunofluorescence and molecular assay targeting methods. In addition, γH2AX cytochrome c expressions were evaluated immunohistochemical immunofluorescent methods samples positive for BPV agent. this study, 100 specimens that did not show any macroscopic papilloma findings collected. Testicular tissues collected examined using histologically molecularly present immunopositivity was detected 6 (6%) with IHC method 11 (11%) IF method. positivity four (4%) PCR determined be also showed seen two BPV‐positive method, observed agent Cytochrome found all When evaluated, cattle have papillomas revealed first time. It thought caused double‐stranded DNA breaks increased expression.

Language: Английский

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Evaluation of local expressions of acute phase proteins in white muscle disease in lambs by the immunohistochemical method

Revista Científica de la Facultad de Ciencias Veterinarias, Journal Year: 2024, Volume and Issue: XXXIV(1), P. 1 - 7

Published: Jan. 2, 2024

White muscle disease (WMD) and/or nutritional muscular dystrophy is defined as a with generally acute course that results in degeneration and necrosis of the heart muscle. In this study, it was aimed to reveal local tissue expressions phase proteins such C–reactive protein (CRP), Serum amyloid–A (SAA) Haptoglobin (Hp) lambs WMD. The study material consisted 27, one–six months old lamb tissues, 6 which were healthy 21 necropsied, relevant samples taken into neutral formaldehyde. Afterwards, paraffin blocks obtained by going through routine follow–up processes. Sections from stained Hematoxylin–Eosin (H–E) Immunohistochemical methods. Histopathologically, control groups exhibited normal histology. Hyaline degeneration, Zenker necrosis, calcification, inflammatory cell infiltration an increase connective detected tissues Lambs WMD had significantly increased CRP SAA compared (P<0.01). However, there no difference between Hp (P>0.05). conclusion, CRP, identified immunohistochemically for first time. It possible say may play important roles pathophysiology provide more sensitive diagnosis prognosis disease.

Language: Английский

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Therapeutic applications of eucalyptus essential oils

Inflammopharmacology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

Abstract Eucalyptus essential oils (EEOs) have gained significant attention recently anticipated to their broad range of prospective benefits in various biological applications. They been proven strong antibacterial properties against a variety bacteria, fungi, and viruses. This makes them valuable combating infections supporting overall hygiene. The active compounds present these can help alleviate inflammation, making addressing inflammatory conditions such as arthritis, respiratory ailments, skin disorders. Respiratory health are another prominent aspect EEOs. Inhalation promote clear airways, relieve congestion, ease symptoms like coughs, colds, sinusitis. often utilized inhalation therapies chest rubs. be used topically or massage muscle joint pain. Furthermore, shown potential wound healing. Their antimicrobial activity helps prevent infection, while anti-inflammatory analgesic contribute reducing inflammation pain associated with wounds. In aromatherapy, EEOs renowned for invigorating uplifting qualities, promoting mental clarity, relaxation, stress relief. Overall, hold great promise applications, offering natural versatile approach well-being. Continued research exploration therapeutic will further unveil broaden applications fields.

Language: Английский

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Taxifolin attenuates cisplatin-induced kidney damage in rats via suppressing p53 and iNOS

Etlik Veteriner Mikrobiyoloji Dergisi, Journal Year: 2024, Volume and Issue: 35(1), P. 1 - 7

Published: May 22, 2024

Cisplatin (CP) is a platinum-based anticancer drug used to treat many different solid tumors. Although CP has strong properties, its clinical use limited due side effects such as ototoxicity, neurotoxicity, myelosuppression and nephrotoxicity. Taxifolin (Tax) reported exhibit various possess anti-inflammatory, antioxidant, antimicrobial, antiviral anticancer. In this study, we aimed investigate the possible of Tax on CP-induced This study consisted Control (C), (Tax), + (CP Tax) groups, there were 6 rats in each group. was administered intraperitoneally (i.p.) single dose 7 mg/kg, orally at 50 mg/kg for consecutive days. Histopathologically, significant changes tubular epithelial degeneration necrosis, dilatation, inflammatory cell infiltrates, hyaline cast, glomerular atrophy detected It seen that CP+Tax group significantly reduced histopathological (p

Language: Английский

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Determination of local expressions of IGF-1, LC3B and NF-kB in white muscle disease in lambs by immunohistochemical method

Journal of Advances in VetBio Science and Techniques, Journal Year: 2024, Volume and Issue: unknown

Published: May 8, 2024

White muscle disease (WMD) is also known as Stiff Lamb Disease or Nutritional Muscular Dystrophy. Selenium and/or Vitamin E deficiency constitutes the etiology of disease. This study aimed to immunohistochemically evaluate local protein expressions Nuclear factor kappa B (NF-kB), Insulin-like growth factor-1 (IGF-1) and Microtubule-related 1A/1B-light chain 3 beta (LC3B) in WMD. The material consisted 15 WMD, 6 healthy lamb heart samples. tissues autopsied lambs were subjected routine tissue processing paraffin blocks obtained. Then, it was stained with Hematoxylin-Eosin immunohistochemical methods. Control group had normal macroscopic appearance. Macroscopically, hyaline degeneration zenker’s necrosis, calcification areas observed WMD tissues. Microscopically, degenerative necrotic fibers, areas, fibrosis, mononuclear cell infiltrates macrophage detected Immunohistochemically, significant increases IGF-1 (p

Language: Английский

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Methylene Blue confers protection against hepatotoxicity induced by Cisplatin; the involvement of the interactions between iron-sensing proteins, NCOA4-mediated ferritinophagy, and oxidative stress

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 28, 2024

Cisplatin (Cisp) is a widely used cancer treatment that effective and frequently included in plans. Treatment plans use large doses of Cisp or cumulative dosages, however, may cause liver damage. This study investigated the protective effects Methylene Blue (Meth) against ‎cisplatin-induced hepatotoxicity male Wistar Albino rats. Rats were divided into four groups: control, cisplatin (CP, 8 mg/kg, IP), two Meth-‎treated groups (25 mg/kg 50 IP) with CP‎. administration (8 significantly impaired function, increased ‎oxidative stress, promoted apoptosis, disrupted iron homeostasis. ‎Meth at 25 demonstrated dose-dependent ‎hepatoprotective effects. The higher dose (50 mg/kg) Meth effectively restored ‎function markers, antioxidant status, regulatory proteins (IRP2, TRF1, ferritin NCOA4) addition to ferritinophagy levels comparable ‎with control group. reduced lipid peroxidation, inhibited ‎apoptotic pathways, modulated iron-related gene expression. ‎consistently outperformed across all measured parameters. These ‎findings suggest Blue, particularly doses, serve as ‎promising therapeutic agent mitigating cisplatin-induced by reducing regulating homeostasis, inhibiting apoptotic pathways.‎

Language: Английский

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Eucalyptol (1.8-cineole) attenuates gentamicin-induced liver injury

Etlik Veteriner Mikrobiyoloji Dergisi, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 2, 2024

Gentamicin (GM), which is in the aminoglycoside antibiotic class, frequently preferred today treatment of diseases caused by gram-negative bacteria. However, its significant side effects on liver and kidney functions limit clinical usefulness. The antioxidant anti-inflammatory medical activities eucalyptol (EUC, 1.8-cineole) have been reported different studies. This study aimed to evaluate EUC GM-induced hepatotoxicity. groups are consisted control (C), EUC, GM + there were 7 rats each group. At end study, euthanized under appropriate conditions samples collected biochemical, histopathological immunohistochemical analyzes performed. It was determined that a important increase serum alanine aminotransferase (ALT), aspartate transferase (AST) gamma-glutamyl (GGT) enzymes group relative C (p

Language: Английский

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Evaluation of The Effects of Tarantula Cubensis Alcohol Extract and Sorafenib Treatments on P21 Protein, Total Antioxidant Capacity and Metabolic Profile in Experimental Rats Hepatocellular Carcinoma

Manas Journal of Agriculture Veterinary and Life Sciences, Journal Year: 2024, Volume and Issue: unknown, P. 201 - 209

Published: Oct. 22, 2024

Hepatocellular carcinoma (HCC) is defined as the sixth most common cancer type and third in terms of cancer-related deaths. Tarantula cubensis alcohol extract (TCAE, Theranekron) a homeopathic medicine frequently used veterinary treatment papilloma, mammary adenocarcinoma necrotic disorders. The present study aimed to reveal effectiveness TCAE Sorafenib (S) HCC induced by Diethylnitrosamine (DEN) N-nitrosomorpholine (NMOR)-induced rats. Rats were randomly divided into 7 groups: Control (C), + (CT), S (CS), Cancer (CC), CC+TCAE (CCT), CC+S (CCS), CC+TCAE+S (CCTS). In CC group, values for glucose, triglyceride (TG) total antioxidant capacity (TAC) found be significantly higher than all other groups (p&lt;0.001), while p21 levels lower (p&lt;0.05). It determined an increase serum (p&lt;0.05) significant decrease glucose TG (p&lt;0.001) CCT, CCS CCTS compared group. Histopathological examination revealed that group showed morphology, caused tumor incidence size. As result, it can said alone and/or combined with chemotherapy drugs antiproliferative effects on cells HCC. combination therapy may potentially synergize improve magnitude durability antitumor responses patients

Language: Английский

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