Scientific Reports,
Journal Year:
2018,
Volume and Issue:
8(1)
Published: Aug. 10, 2018
Acquiring
information
on
the
precise
distribution
of
a
tumor
is
essential
to
evaluate
intratumoral
heterogeneity.
Conventional
hematoxylin
and
eosin
staining,
which
has
been
used
by
pathologists
for
more
than
100
years,
gold
standard
diagnosis.
However,
it
difficult
stain
entire
tissues
with
then
acquire
three-dimensional
cells
in
solid
tumors
due
difficulties
staining
rinsing.
In
this
paper,
we
propose
modified
method,
delipidation
ultrasound
waves
were
applied
enhance
tissue
permeability
accelerate
dye
diffusion.
This
improved
method
termed
iHE
(intact
staining).
We
intact
organs
mice,
sectioned
imaged
sequentially.
The
results
showed
that
whole
was
stained
homogeneously.
Combined
micro-optical
sectioning
tomography
(MOST),
can
be
3D
volume
imaging
heterogeneity
spatially.
Therefore,
may
help
accurately
diagnose
invasion
stage
guide
clinical
treatments.
Cell Research,
Journal Year:
2018,
Volume and Issue:
28(8), P. 803 - 818
Published: May 29, 2018
Tissue
clearing
technique
enables
visualization
of
opaque
organs
and
tissues
in
3-dimensions
(3-D)
by
turning
tissue
transparent.
Current
methods
are
restricted
limited
types
that
can
be
cleared
with
each
individual
protocol,
which
inevitably
led
to
the
presence
blind-spots
within
whole
body
or
parts
imaging.
Hard
including
bones
teeth
still
most
difficult
cleared.
In
addition,
loss
endogenous
fluorescence
remains
a
major
concern
for
solvent-based
methods.
Here,
we
developed
polyethylene
glycol
(PEG)-associated
solvent
system
(PEGASOS),
rendered
nearly
all
transparent
preserved
fluorescence.
Bones
could
turned
invisible
after
clearing.
The
PEGASOS
method
adult
mouse
were
able
image
an
head
composed
bones,
teeth,
brain,
muscles,
other
no
blind
areas.
transparency
enabled
us
reconstruct
intact
mandible,
femur,
knee
joint
3-D.
managed
brain
at
sub-cellular
resolution
trace
neurons
axons
over
long
distance.
We
also
visualized
dorsal
root
ganglions
directly
through
vertebrae.
Finally,
revealed
distribution
pattern
neural
network
3-D
marrow
space
bone.
These
results
suggest
is
useful
tool
general
biomedical
research.
Cell Reports,
Journal Year:
2018,
Volume and Issue:
24(8), P. 2196 - 2210.e9
Published: Aug. 1, 2018
We
describe
a
strategy
for
developing
hydrophilic
chemical
cocktails
tissue
delipidation,
decoloring,
refractive
index
(RI)
matching,
and
decalcification,
based
on
comprehensive
profiling.
More
than
1,600
chemicals
were
screened
by
high-throughput
evaluation
system
each
process.
The
profiling
revealed
important
factors:
salt-free
amine
with
high
octanol/water
partition-coefficient
(logP)
N-alkylimidazole
aromatic
amide
RI
protonation
of
phosphate
ion
decalcification.
strategic
integration
optimal
provided
series
CUBIC
(clear,
unobstructed
brain/body
imaging
computational
analysis)
protocols,
which
efficiently
clear
mouse
organs,
body
including
bone,
even
large
primate
human
tissues.
updated
protocols
are
scalable
reproducible,
they
enable
three-dimensional
the
mammalian
This
represents
future
paradigm
rational
design
clearing
that
can
be
used
Cell Reports,
Journal Year:
2017,
Volume and Issue:
20(1), P. 236 - 250
Published: July 1, 2017
Highlights•RI-optimized
CUBIC
protocol
enables
whole-body
examination
of
cancer
models•CUBIC
is
applicable
to
analysis
micrometastases
at
single-cell
resolution•CUBIC
bridges
the
resolution
gap
between
in
vivo
imaging
and
histology•CUBIC-cancer
useful
for
profiling
biological
processes
whole
organsSummaryStochastic
proliferative
events
initiated
from
a
single
cell
can
disrupt
homeostatic
balance
lead
fatal
disease
such
as
metastasis.
To
overcome
metastasis,
it
necessary
detect
quantify
sparsely
distributed
metastatic
cells
throughout
body
early
stages.
Here,
we
demonstrate
that
clear,
unobstructed
brain/body
cocktails
computational
(CUBIC)-based
(CUBIC-cancer)
with
refractive
index
(RI)-optimized
comprehensive
organs.
We
applied
CUBIC-cancer
13
mouse
models
using
nine
lines
spatiotemporal
quantification
progression
resolution.
suggests
epithelial-mesenchymal
transition
promotes
not
only
extravasation
but
also
survival
sites.
pharmacotherapeutic
anti-tumor
drugs.
compatible
bioluminescence
2D
histology.
suggest
scalable
analytical
pipeline
these
three
modalities
may
contribute
addressing
currently
incurable
diseases.Graphical
abstract
