Highly-multiplexed volumetric mapping with Raman dye imaging and tissue clearing DOI
Lixue Shi, Mian Wei, Yupeng Miao

et al.

Nature Biotechnology, Journal Year: 2021, Volume and Issue: 40(3), P. 364 - 373

Published: Oct. 4, 2021

Language: Английский

CODEX multiplexed tissue imaging with DNA-conjugated antibodies DOI
Sarah Black, Darci J. Phillips, John W. Hickey

et al.

Nature Protocols, Journal Year: 2021, Volume and Issue: 16(8), P. 3802 - 3835

Published: July 2, 2021

Language: Английский

Citations

438
Near-infrared luminescence high-contrast in vivo biomedical imaging DOI Open Access
Ying Chen, Shangfeng Wang, Fan Zhang

et al.

Nature Reviews Bioengineering, Journal Year: 2023, Volume and Issue: 1(1), P. 60 - 78

Published: Jan. 19, 2023

Language: Английский

Citations

277
Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues DOI Creative Commons
Etsuo A. Susaki,

Chika Shimizu,

Akihiro Kuno

et al.

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: April 27, 2020

Abstract Whole-organ/body three-dimensional (3D) staining and imaging have been enduring challenges in histology. By dissecting the complex physicochemical environment of system, we developed a highly optimized 3D pipeline based on CUBIC. Based our precise characterization biological tissues as an electrolyte gel, experimentally evaluated broad conditions by using artificial tissue-mimicking material. The combination allows bottom-up design superior protocol that can uniformly label whole adult mouse brains, marmoset brain hemisphere, ~1 cm 3 tissue block postmortem human cerebellum, entire infant body with dozens antibodies cell-impermeant nuclear stains. whole-organ images collected light-sheet microscopy are used for computational analyses comparison analysis between species. This pipeline, named CUBIC-HistoVIsion, thus offers advanced opportunities organ- organism-scale histological multicellular systems.

Language: Английский

Citations

260
Light sheet fluorescence microscopy DOI
Ernst H. K. Stelzer, Frederic Strobl, Bo-Jui Chang

et al.

Nature Reviews Methods Primers, Journal Year: 2021, Volume and Issue: 1(1)

Published: Nov. 3, 2021

Language: Английский

Citations

206
In situ genome sequencing resolves DNA sequence and structure in intact biological samples DOI Open Access

Andrew C. Payne,

Zachary Chiang, Paul Reginato

et al.

Science, Journal Year: 2021, Volume and Issue: 371(6532)

Published: Feb. 25, 2021

Understanding genome organization requires integration of DNA sequence and three-dimensional spatial context; however, existing genome-wide methods lack either base pair resolution or direct localization. Here, we describe in situ sequencing (IGS), a method for simultaneously imaging genomes within intact biological samples. We applied IGS to human fibroblasts early mouse embryos, spatially localizing thousands genomic loci individual nuclei. Using these data, characterized parent-specific changes structure across embryonic stages, revealed single-cell chromatin domains zygotes, uncovered epigenetic memory global chromosome positioning embryos. These results demonstrate how can directly connect length scales from single pairs whole organisms.

Language: Английский

Citations

184
Engineering the multiscale complexity of vascular networks DOI Open Access
Colleen E. O’Connor, Eileen L. Brady, Ying Zheng

et al.

Nature Reviews Materials, Journal Year: 2022, Volume and Issue: 7(9), P. 702 - 716

Published: May 31, 2022

Language: Английский

Citations

149
A roadmap for the Human Developmental Cell Atlas DOI Open Access
Muzlifah Haniffa, Deanne Taylor, Sten Linnarsson

et al.

Nature, Journal Year: 2021, Volume and Issue: 597(7875), P. 196 - 205

Published: Sept. 8, 2021

Language: Английский

Citations

144
Tissue clearing DOI
Douglas S. Richardson, Webster Guan, Katsuhiko Matsumoto

et al.

Nature Reviews Methods Primers, Journal Year: 2021, Volume and Issue: 1(1)

Published: Dec. 16, 2021

Language: Английский

Citations

109
Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues DOI Creative Commons
Xiaoping Zhu, Tianyi Xu, Peng Chen

et al.

Frontiers in Chemistry, Journal Year: 2022, Volume and Issue: 9

Published: Feb. 3, 2022

Compared with conventional optical microscopy techniques, mass spectrometry imaging (MSI) or (IMS) is a powerful, label-free analytical technique, which can sensitively and simultaneously detect, quantify, map hundreds of biomolecules, such as peptides, proteins, lipid, other organic compounds in cells tissues. So far, although several soft ionization desorption electrospray (DESI) secondary ion (SIMS) have been used for matrix-assisted laser desorption/ionization (MALDI) still the most widespread MSI scanning method. Here, we aim to provide comprehensive review MALDI-MSI an emphasis on its advances instrumentation, methods, application, future directions single cell biological

Language: Английский

Citations

81
Progress in multicellular human cardiac organoids for clinical applications DOI Creative Commons
Hyeonyu Kim,

Roger D. Kamm,

Gordana Vunjak‐Novakovic

et al.

Cell stem cell, Journal Year: 2022, Volume and Issue: 29(4), P. 503 - 514

Published: April 1, 2022

Language: Английский

Citations

81