Tissue clearing and its applications in neuroscience

Nature reviews. Neuroscience, Journal Year: 2020, Volume and Issue: 21(2), P. 61 - 79

Published: Jan. 2, 2020

Language: Английский

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Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells

Nature Biotechnology, Journal Year: 2020, Volume and Issue: 38(12), P. 1421 - 1430

Published: Dec. 1, 2020

Language: Английский

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A tissue-like neurotransmitter sensor for the brain and gut

Nature, Journal Year: 2022, Volume and Issue: 606(7912), P. 94 - 101

Published: June 1, 2022

Language: Английский

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Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: April 27, 2020

Abstract Whole-organ/body three-dimensional (3D) staining and imaging have been enduring challenges in histology. By dissecting the complex physicochemical environment of system, we developed a highly optimized 3D pipeline based on CUBIC. Based our precise characterization biological tissues as an electrolyte gel, experimentally evaluated broad conditions by using artificial tissue-mimicking material. The combination allows bottom-up design superior protocol that can uniformly label whole adult mouse brains, marmoset brain hemisphere, ~1 cm 3 tissue block postmortem human cerebellum, entire infant body with dozens antibodies cell-impermeant nuclear stains. whole-organ images collected light-sheet microscopy are used for computational analyses comparison analysis between species. This pipeline, named CUBIC-HistoVIsion, thus offers advanced opportunities organ- organism-scale histological multicellular systems.

Language: Английский

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Cellular and Molecular Probing of Intact Human Organs

Cell, Journal Year: 2020, Volume and Issue: 180(4), P. 796 - 812.e19

Published: Feb. 1, 2020

Language: Английский

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Advanced CUBIC tissue clearing for whole-organ cell profiling

Nature Protocols, Journal Year: 2019, Volume and Issue: 14(12), P. 3506 - 3537

Published: Nov. 20, 2019

Language: Английский

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Whole-Brain Profiling of Cells and Circuits in Mammals by Tissue Clearing and Light-Sheet Microscopy

Neuron, Journal Year: 2020, Volume and Issue: 106(3), P. 369 - 387

Published: May 1, 2020

Language: Английский

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Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues

Nature Communications, Journal Year: 2019, Volume and Issue: 10(1)

Published: July 4, 2019

Recent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural molecular information from intact tissues. However, current microscopes imposed constraints on the size, shape, number of specimens, compatibility with various protocols. Here we present a multi-immersion open-top microscope that enables simple mounting multiple specimens processed variety protocols, which will facilitate wide adoption by preclinical researchers clinical laboratories. In particular, geometry provides unsurpassed versatility interface range accessory technologies future.

Language: Английский

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High-dimensional cell-level analysis of tissues with Ce3D multiplex volume imaging

Nature Protocols, Journal Year: 2019, Volume and Issue: 14(6), P. 1708 - 1733

Published: April 26, 2019

Language: Английский

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Generation of a p16 Reporter Mouse and Its Use to Characterize and Target p16high Cells In Vivo

Cell Metabolism, Journal Year: 2020, Volume and Issue: 32(5), P. 814 - 828.e6

Published: Sept. 18, 2020

Language: Английский

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