Functional and Structural Diversity of Insect Glutathione S-transferases in Xenobiotic Adaptation

International Journal of Biological Sciences, Journal Year: 2022, Volume and Issue: 18(15), P. 5713 - 5723

Published: Jan. 1, 2022

As a superfamily of multifunctional enzymes that is mainly associated with xenobiotic adaptation, glutathione S-transferases (GSTs) facilitate insects' survival under chemical stresses in their environment.GSTs confer adaptation through direct metabolism or sequestration xenobiotics, and/or indirectly by providing protection against oxidative stress induced exposure.In this article, comprehensive overview current understanding on the versatile functions insect GSTs detoxifying compounds presented.The diverse structures different classes GSTs, specifically spatial localization and composition amino acid residues constituted active sites are also summarized.Recent availability whole genome sequences numerous species, accompanied RNA interference, X-ray crystallography, enzyme kinetics site-directed mutagenesis techniques have significantly enhanced our functional structural diversity GSTs.

Language: Английский

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New and emerging mechanisms of insecticide resistance

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 63, P. 101184 - 101184

Published: March 6, 2024

Language: Английский

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Self-assembled co-delivery nanoplatform for increasing the broad-spectrum susceptibility of fall armyworm toward insecticides

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Unscientific application of insecticides has led to severe resistance pests almost all classes insecticides. Enhanced detoxification is the most common mechanism for this kind resistance. Object: Fall armyworm (FAW) developed insecticide resistance, which often linked overexpression genes. Herein, a multicomponent nano-pesticide designed increase its broad-spectrum susceptibility toward Regulatory function nuclear factor erythroid 2-related 2 (Nrf2) in was confirmed using transcriptome sequencing, quantitative real-time PCR and enzyme activity measurement. A star polycation (SPc) adopted construct pesticide/SPc/complex, whose self-assembly characterization were examined isothermal titration calorimetry, dynamic light scattering transmission electron microscope. The delivery efficiency SPc-loaded dsRNA vitro vivo fluorescent tracer technique. created through integration bacterial expression system nano-delivery system, bioactivity tested laboratory field. We crucial role Nrf2 regulating FAW, silencing could decrease gene susceptibility. then applied SPc self-assemble nanoplatform delivering double-stranded RNA (dsRNA) pesticide simultaneously. Nano-sized pesticide/SPc/dsRNA complex exhibited high vivo. Excitingly, insecticidal activities pesticide/SPc/dsNrf2 complexes remarkably improved with normalized synergistic ratios 5.43-6.25 chlorantraniliprole, 4.45-15.00 emamectin benzoate, 6.75-15.00 spinetoram. Finally, we (pesticide/SPc/dsNrf2 complex) system. This approach excellent leaf protection pest control efficacy. between nanometerization improvement offers promising strategy activity. Our study provides revolutionary universal decease doses.

Language: Английский

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Weight of evidence assessment from field studies on effects of the insecticide sulfoxaflor on hymenopteran pollinators: sulfoxaflor environmental science review part V

Journal of Toxicology and Environmental Health Part B, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 32

Published: March 24, 2025

Field studies involve combinations of exposure, natural dynamics, and effects in agricultural environments. To be more realistic, field focussed on pollinating insects must consider the details biology, life history, behavior, pollination ecology test species. While expensive time-consuming, these tests provide most realistic information, especially for social insects, but are valuable solitary bee species as well. They than laboratory because they determine combined stressors including weather, food availability, parasites, pathogens with anthropogenic stressors, such pesticide treatment itself, within agroecosystem landscapes. Twenty-four conducted bees to support registration sulfoxaflor published work included, a standardized rating system quality relevance was used. The included Apis mellifera L. Bombus terrestris Osmia bicornis results show that, when SFX products applied at highest labeled application rate actively foraging or fed syrup equivalent rates, minor temporary. Sublethal lethargy, disorientation, reduced body mass emergence. No new modes action no treatment-related brood rearing were found.

Language: Английский

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Genome sequences of four Ixodes species expands understanding of tick evolution

BMC Biology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 21, 2025

Language: Английский

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Weight of evidence assessment of effects of sulfoxaflor on aquatic invertebrates: sulfoxaflor environmental science review part II

Journal of Toxicology and Environmental Health Part B, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 29

Published: March 25, 2025

Effects of sulfoxaflor (SFX) on aquatic invertebrates were assessed by comparing concentrations predicted to occur in or measured surface waters thresholds for adverse effects. Due the specific mode toxic action, fishes are relatively tolerant effects SFX. Daphnia magna with an LC50 378 mg SFX L-1 (SD = 19.13) was similarly SFX, while LOEC >110 L-1. A threshold insects, based chironomid midge, C. tentans, had LOAEL and NOAEL values 0.0455 0.0618 L-1, respectively. The acute-to-chronic ratio 18. Simulation models parameters selected a range applications crops environmental (EECs) from 2.2 7.7 µg Based these EECs, maximum hazard quotient (HQ) 0.11, which is less than US EPA level concern (LOC) 0.5, would normally be trigger regulatory action higher-tier assessments. risks posed organisms de minimis. Hazard quotients EEC standard, USEPA farm pond estimated use Pesticides Water Calculator (PWC version 1.52) scenarios application rates cotton insects similar those other insecticides including neonicotinoids organophosphorus compounds.

Language: Английский

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A quantitative Apis mellifera hazard and risk assessment model (AMHRA) illustrated with the insecticide sulfoxaflor: sulfoxaflor environmental science review part VI

Journal of Toxicology and Environmental Health Part B, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 29

Published: March 26, 2025

In this paper, conceptual models of the exposure pathways outside hive and in-hive distribution pesticide residues brought to honeybee are presented. The model is based on natural life history, behavior diet individual honeybees (Apis mellifera). Receptor groups bees with similar diets potential defined. From model, a quantitative A. mellifera hazard risk assessment (AMHRA) developed illustrated using sulfoxaflor (SFX) as case study. estimates receptor within colony via various routes exposure. user selects deterministic mode obtain quotients (HQ) or probabilistic (RQ). was run in concentrations nectar pollen from field experiment which SFX applied cotton crops at highest permitted application rate 101 g a.i. ha-1. Acute chronic HQ values were calculated for adult larval groups. results showed that single following label directions not hazardous honeybees. described but run.

Language: Английский

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Pesticide resistance in arthropods: Ecology matters too

Ecology Letters, Journal Year: 2022, Volume and Issue: 25(8), P. 1746 - 1759

Published: June 21, 2022

Pesticide resistance development is an example of rapid contemporary evolution that poses immense challenges for agriculture. It typically evolves due to the strong directional selection pesticide treatments exert on herbivorous arthropods. However, recent research suggests some species are more prone evolve than others their evolutionary history and standing genetic variation. Generalist might develop especially rapidly pre-adaptation handle a wide array plant allelochemicals. Moreover, has shown adaptation novel host plants could lead increased resistance. Exploring such cross-resistance between range from ecological perspective needed understand its causes consequences better. Much has, however, been devoted molecular mechanisms underlying while both contexts facilitate have under-studied. Here, we take eco-evolutionary approach discuss circumstances may in arthropods plant-arthropod interactions target non-target interactions. Furthermore, suggest future avenues practical implications understanding evolution.

Language: Английский

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Comparative analysis of the detoxification gene inventory of four major Spodoptera pest species in response to xenobiotics

Insect Biochemistry and Molecular Biology, Journal Year: 2021, Volume and Issue: 138, P. 103646 - 103646

Published: Aug. 29, 2021

Language: Английский

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Divergent amplifications of CYP9A cytochrome P450 genes provide two noctuid pests with differential protection against xenobiotics

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(37)

Published: Sept. 5, 2023

Here, we provide mechanistic support for the involvement of CYP9A subfamily cytochrome P450 monooxygenases in detoxification host plant defense compounds and chemical insecticides Spodoptera exigua frugiperda . Our comparative genomics shows that a large cluster genes occurs two species but with significant differences its contents, including several species-specific duplicates substantial sequence divergence, both between orthologs duplicates. Bioassays CRISPR-Cas9 knockouts clusters show that, collectively, CYP9As can detoxify furanocoumarin (imperatorin xanthotoxin) representing three different chemotypes (pyrethroids, avermectins, oxadiazines). However, vitro metabolic assays heterologously expressed products individual particular activities against these compounds. We also find tight genetic linkage high levels pyrethroid resistance field strains species. propose their divergent amplifications have not only contributed to development broad ranges over long evolutionary timeframes supplied them diverse options evolving very recent past.

Language: Английский

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