Annual Review of Immunology,
Journal Year:
2021,
Volume and Issue:
39(1), P. 131 - 166
Published: Jan. 22, 2021
Dendritic
cells
(DCs)
possess
the
ability
to
integrate
information
about
their
environment
and
communicate
it
other
leukocytes,
shaping
adaptive
innate
immunity.
Over
years,
a
variety
of
cell
types
have
been
called
DCs
on
basis
phenotypic
functional
attributes.
Here,
we
refocus
attention
conventional
(cDCs),
discrete
lineage
by
ontogenetic
gene
expression
criteria
that
best
corresponds
originally
described
in
1970s.
We
summarize
current
knowledge
mouse
human
cDC
subsets
describe
hematopoietic
development
hope
our
effort
review
basic
features
biology
distinguish
cDCs
from
related
brings
fore
remarkable
properties
this
type
while
shedding
some
light
seemingly
inordinate
complexity
DC
field.
Cytometry Part A,
Journal Year:
2024,
Volume and Issue:
105(6), P. 430 - 436
Published: April 18, 2024
Abstract
We
report
the
development
of
an
optimized
50‐color
spectral
flow
cytometry
panel
designed
for
in‐depth
analysis
immune
system
in
human
blood
and
tissues,
with
goal
maximizing
amount
information
that
can
be
collected
using
currently
available
platforms.
established
tested
this
peripheral
mononuclear
cells
(PBMCs),
but
included
CD45
to
enable
its
future
use
tissue
samples.
The
contains
lineage
markers
all
major
cell
subsets,
extensive
set
phenotyping
focused
on
activation
differentiation
status
T
dendritic
(DC)
compartment.
outline
biological
insight
gained
from
simultaneous
measurement
such
a
large
number
proteins
propose
approach
provides
unique
opportunity
comprehensive
exploration
samples
limited
cells.
Of
note,
we
compatible
sorting
further
downstream
applications.
Furthermore,
facilitate
wide‐spread
implementation
across
different
cohorts
samples,
trimmed‐down
45‐color
version
which
used
Finally,
generate
panel,
utilized
not
only
existing
design
guidelines,
also
developed
new
metrics
systematically
identify
optimal
combination
50
fluorochromes
evaluate
fluorochrome‐specific
resolution
context
unmixing
matrix.
Cell Communication and Signaling,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: Feb. 6, 2024
Abstract
The
changes
in
T
regulatory
cell
(Treg)
and
helper
(Th)
17
ratios
holds
paramount
importance
ensuring
internal
homeostasis
disease
progression.
Recently,
novel
subsets
of
Treg
Th17,
namely
IL-17-producing
IL-10-producing
Th17
have
been
identified.
are
widely
considered
as
the
intermediates
during
Treg/Th17
transformation.
These
“bi-functional”
cells
exhibit
plasticity
demonstrated
with
important
roles
multiple
physiological
functions
processes.
Yin
Yang
represent
opposing
aspects
phenomena
according
to
ancient
Chinese
philosophy
“Yin-Yang”
theory.
Furthermore,
can
transform
into
Yang,
vice
versa,
under
specific
conditions.
This
theory
has
used
describe
contrasting
immune
molecules.
Therefore,
immune-activating
populations
(Th17,
M1
macrophage,
etc.)
overreaction
(inflammation,
autoimmunity)
be
while
immunosuppressive
(Treg,
M2
immunosuppression
(tumor,
immunodeficiency)
Yin.
However,
another
connotation
theory,
conversion
between
rarely
documented
studies.
discovery
enriches
meaning
further
promotes
relationship
modern
immunology.
Besides,
illustrating
mechanisms
governing
their
differentiation
provides
valuable
insights
underlying
dynamically
changing
statement
health
diseases.
The Journal of Experimental Medicine,
Journal Year:
2024,
Volume and Issue:
221(4)
Published: March 4, 2024
T
helper
2
(Th2)
responses
protect
against
pathogens
while
also
driving
allergic
inflammation,
yet
how
large-scale
Th2
are
generated
in
tissue
context
remains
unclear.
Here,
we
used
quantitative
imaging
to
investigate
early
differentiation
within
lymph
nodes
(LNs)
following
cutaneous
allergen
administration.
Contrary
current
models,
observed
extensive
activation
and
"macro-clustering"
of
cells
with
migratory
type-2
dendritic
(cDC2s),
generating
specialized
Th2-promoting
microenvironments.
Macro-clustering
was
integrin-mediated
promoted
localized
cytokine
exchange
among
reinforce
differentiation,
which
contrasted
the
behavior
during
Th1
responses.
Unexpectedly,
formation
macro-clusters
dependent
on
site
skin
sensitization.
Differences
between
sites
were
driven
by
divergent
states
cDC2
from
different
dermal
tissues,
enhanced
costimulatory
molecule
expression
Th2-generating
LNs
promoting
prolonged
cell
activation,
macro-clustering,
sensing.
Thus,
generation
dedicated
priming
microenvironments
through
signaling
initiates
vivo
occurs
a
site-specific
manner.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(11), P. 5774 - 5774
Published: May 26, 2024
Glioblastoma
is
the
most
common
malignant
primary
brain
tumor
in
adult
population,
with
an
average
survival
of
12.1
to
14.6
months.
The
standard
treatment,
combining
surgery,
radiotherapy,
and
chemotherapy,
not
as
efficient
we
would
like.
However,
current
possibilities
are
no
longer
limited
therapies
due
rapid
advancements
biotechnology.
New
methods
enable
a
more
precise
approach
by
targeting
individual
cells
antigens
overcome
cancer.
For
treatment
glioblastoma,
these
gamma
knife
therapy,
proton
beam
tumor-treating
fields,
EGFR
VEGF
inhibitors,
multiple
RTKs
PI3K
pathway
inhibitors.
In
addition,
increasing
understanding
role
immune
system
tumorigenesis
ability
identify
tumor-specific
helped
develop
immunotherapies
GBM
cells,
including
CAR-T,
CAR-NK
dendritic
checkpoint
Each
described
has
its
advantages
disadvantages
faces
problems,
such
inefficient
crossing
blood-brain
barrier,
various
neurological
systemic
side
effects,
escape
mechanism
tumor.
This
work
aims
present
modern
treatments
glioblastoma.
