Hydrogen gas therapy: A promising approach for sepsis management post-burn injury by modulating inflammation, oxidative stress, and wound healing DOI Open Access
Yu Pan, Nan Hong,

Genwang Wang

et al.

CytoJournal, Journal Year: 2025, Volume and Issue: 22, P. 46 - 46

Published: April 25, 2025

Objective Burns refers to a severe form of trauma that often leads localized and systemic inflammatory responses, oxidative stress, immune dysfunction. Patients with burns are highly susceptible the development postburn sepsis, condition influenced by multiple factors, such as bacterial infection burn wound, alterations in status, excessive release mediators. This study aimed investigate mechanisms which hydrogen gas treatment exerts its effects on focus influence wound healing. Material Methods work employed vitro assays Sprague–Dawley (SD) rat skin fibroblasts (RSFs) assess serum cell proliferation through methylthiazolyldiphenyltetrazolium bromide apoptosis flow cytometry Annexin V-fluorescein isothiocyanate/propidium iodide staining. In addition, an enzyme-linked immunosorbent assay was performed quantify cytokines stress markers treated serum. Western blotting (WB) analysis conducted signaling pathway modulation. The sepsis models SD rats were segregated into three experimental groups: healthy normal control group, + (2%) group. Wound healing monitored, contraction rates recorded histological assessments using hematoxylin eosin Masson’s trichrome staining evaluate tissue repair collagen deposition. Results showed reduced fibroblast increased ( P < 0.01), mitigated rescuing viability reducing 0.01). Enzyme-linked revealed serum-induced increases levels markers, decreases antioxidant enzymes reversed 0.05). WB suggested gas’s anti-inflammatory proliferative modulating pathways vivo , considerably improved healing, accelerated enhanced Plasma analyses indicated local from gas. Conclusion Hydrogen demonstrates potential therapeutic efficacy management promoting These findings provide scientific evidence supporting adjunctive strategy for sepsis.

Language: Английский

Mast Cells as a Potential Target of Molecular Hydrogen in Regulating the Local Tissue Microenvironment DOI Creative Commons
Dmitri Atiakshin, А. А. Костин,

Artem Volodkin

et al.

Pharmaceuticals, Journal Year: 2023, Volume and Issue: 16(6), P. 817 - 817

Published: May 30, 2023

Knowledge of the biological effects molecular hydrogen (H2), gas, is constantly advancing, giving a reason for optimism in several healthcare practitioners regarding management multiple diseases, including socially significant ones (malignant neoplasms, diabetes mellitus, viral hepatitis, mental and behavioral disorders). However, mechanisms underlying H2 are still being actively debated. In this review, we focus on mast cells as potential target at specific tissue microenvironment level. regulates processing pro-inflammatory components cell secretome their entry into extracellular matrix; can significantly affect capacity integrated-buffer metabolism structure immune landscape local microenvironment. The analysis performed highlights developing offers great opportunities translating obtained findings clinical practice.

Language: Английский

Citations

13
The role of hydrogen therapy in Alzheimer's disease management: Insights into mechanisms, administration routes, and future challenges DOI Open Access
Jiaxuan He, Fan Liu, Ting Xu

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 168, P. 115807 - 115807

Published: Oct. 30, 2023

Alzheimer's disease (AD) is a progressive neurodegenerative disorder predominantly affecting the elderly. While conventional pharmacological therapies remain primary treatment for AD, their efficacy limited effectiveness and often associated with significant side effects. This underscores urgent need to explore alternative, non-pharmacological interventions. Oxidative stress has been identified as central player in AD pathology, influencing various aspects including amyloid-beta metabolism, tau phosphorylation, autophagy, neuroinflammation, mitochondrial dysfunction, synaptic dysfunction. Among emerging non-drug approaches, hydrogen therapy garnered attention its potential mitigating these pathological conditions. review provides comprehensively overview of therapeutic AD. We delve into mechanisms action, administration routes, discuss current challenges future prospects, aim providing valuable insights facilitate clinical application hydrogen-based management.

Language: Английский

Citations

13
Effects of Molecular Hydrogen in the Pathophysiology and Management of Cardiovascular and Metabolic Diseases DOI Creative Commons
Ram B. Singh, Zuzana Sumbalová, Ghizal Fatima

et al.

Reviews in Cardiovascular Medicine, Journal Year: 2024, Volume and Issue: 25(1), P. 33 - 33

Published: Jan. 22, 2024

Diet and lifestyle choices, notably the Western-type diet, are implicated in oxidative stress inflammation, factors that elevate risk of cardiovascular diseases (CVDs) type 2 diabetes mellitus (T2DM). In contrast, Mediterranean rich antioxidants, appears to have protective effects against these risks. This article highlights dual role diet generating molecular hydrogen (H2) gut, H2's subsequent influence on pathophysiology prevention CVD T2DM. Dietary fiber, flavonoids, probiotics contribute production liters H2 functioning as antioxidants neutralize free radicals dampen inflammation. last two decades, mounting evidence has demonstrated both endogenously produced exogenously administered H2, whether via inhalation or H2-rich water (HRW), potent anti-inflammatory across a wide range biochemical pathophysiological processes. Recent studies indicate can hydroxyl nitrosyl radicals, acting cellular antioxidant, thereby reducing inflammation—leading significant decline CVDs metabolic diseases. Clinical experimental research support therapeutic potential interventions such HRW managing However, larger necessary verify therapy management chronic

Language: Английский

Citations

5
Hydrogen exerts neuroprotective effects after subarachnoid hemorrhage by attenuating neuronal ferroptosis and inhibiting neuroinflammation DOI
Zheng Peng, Xiaojian Li, Yan Zhou

et al.

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 215, P. 79 - 93

Published: March 1, 2024

Language: Английский

Citations

5
Hydrogen gas inhalation ameliorates cardiac remodelling and fibrosis by regulating NLRP3 inflammasome in myocardial infarction rats DOI
Chaoqun Nie,

Rentong Zou,

Shuang Pan

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2021, Volume and Issue: 25(18), P. 8997 - 9010

Published: Aug. 16, 2021

Abstract It is noteworthy that prolonged cardiac structural changes and excessive fibrosis caused by myocardial infarction (MI) seriously interfere with the treatment of heart failure in clinical practice. Currently, there are no effective practical means either prevention or treatment. Thus, novel therapeutic approaches critical for long‐term quality life individuals ischaemia. Herein, we aimed to explore protective effect H 2 , a gas signal molecule anti‐oxidative stress anti‐inflammatory effects, on remodelling MI rats, its possible mechanism. First, successfully established model which were then exposed inhalation 2% concentration 28 days (3 hours/day). The results showed hydrogen can significantly improve function reduce area fibrosis. In vitro experiments further proved hypoxia‐induced damage cardiomyocytes alleviate angiotensin II‐induced migration activation fibroblasts. conclusion, herein, illustrated first time ameliorates infarction‐induced rats exert mainly through inhibiting NLRP3‐mediated pyroptosis.

Language: Английский

Citations

29
Molecular Hydrogen Enhances Proliferation of Cancer Cells That Exhibit Potent Mitochondrial Unfolded Protein Response DOI Open Access
Tomoya Hasegawa, Mikako Ito,

Satoru Hasegawa

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(5), P. 2888 - 2888

Published: March 7, 2022

Molecular hydrogen ameliorates pathological states in a variety of human diseases, animal models, and cell but the effects on cancer have been rarely reported. In addition, molecular mechanisms underlying remain mostly unelucidated. We found that enhances proliferation four out seven lines (the responders). The proliferation-promoting were not correlated with basal levels cellular reactive oxygen species. Expression profiling cells showed responders higher gene expression mitochondrial electron transport chain (ETC) molecules than non-responders. mass, superoxide, membrane potential, spare respiratory capacity responders, provoked unfolded protein response (mtUPR). Suppression by rotenone, an inhibitor ETC complex I, was rescued responders. Hydrogen triggers mtUPR induces high activities.

Language: Английский

Citations

20
Redox-Mechanisms of Molecular Hydrogen Promote Healthful Longevity DOI Creative Commons

Md. Habibur Rahman,

Eun‐Sook Jeong, Hae Sun You

et al.

Antioxidants, Journal Year: 2023, Volume and Issue: 12(5), P. 988 - 988

Published: April 24, 2023

Age-related diseases represent the largest threat to public health. Aging is a degenerative, systemic, multifactorial and progressive process, coupled with loss of function eventually leading high mortality rates. Excessive levels both pro- anti-oxidant species qualify as oxidative stress (OS) result in damage molecules cells. OS plays crucial role development age-related diseases. In fact, due oxidation depends strongly on inherited or acquired defects redox-mediated enzymes. Molecular hydrogen (H2) has recently been reported an anti-inflammatory agent for treatment several aging-related diseases, including Alzheimer’s, Parkinson’s, cancer osteoporosis. Additionally, H2 promotes healthy aging, increases number good germs intestine that produce more intestinal reduces through its activities. This review focuses therapeutic neurological manuscript would be useful knowing redox mechanisms promoting healthful longevity.

Language: Английский

Citations

11
Hydrogen-Rich Water Ameliorates Metabolic Disorder via Modifying Gut Microbiota in Impaired Fasting Glucose Patients: A Randomized Controlled Study DOI Creative Commons

Bing Liang,

Le Shi, Dongyue Du

et al.

Antioxidants, Journal Year: 2023, Volume and Issue: 12(6), P. 1245 - 1245

Published: June 9, 2023

Molecular hydrogen (H2) exhibits antioxidant, anti-inflammatory and anti-apoptotic effects, has shown benefits in glucose lipid metabolism certain animal metabolic disorder models. However, the potential of H2 treatment individuals with impaired fasting (IFG) seldom been studied. This randomized controlled study (RCT) aims to investigate effects hydrogen-rich water (HRW) on IFG subjects explore underlying mechanism involved.Seventy-three patients were enrolled a randomized, double-blind, placebo-controlled clinical study. These assigned receive either 1000 mL per day HRW or placebo pure (no infusion) for duration eight weeks. Metabolic parameters fecal gut microbiota assessed at baseline (week 0) week 8. A combined analysis metabolomics intestinal was conducted correlation between effect metabolisms diversity flora IGF patients.Both demonstrated significant reduction blood patients, difference after Among abnormal pre-experimental fatty liver, 62.5% (10/16) group 31.6% (6/19) achieved remission. Furthermore, 16S RNA revealed HRW-modified dysbiosis samples patients. Through Pearson analysis, differential obtained by found be highly correlated nine metabolites.H2 slightly improved abnormalities dysbiosis, providing novel target theoretical basis prevention regulation IFG.

Language: Английский

Citations

11
Hydrogen: A Rising Star in Gas Medicine as a Mitochondria-Targeting Nutrient via Activating Keap1-Nrf2 Antioxidant System DOI Creative Commons
Danyu Cheng, Jiangang Long, Lin Zhao

et al.

Antioxidants, Journal Year: 2023, Volume and Issue: 12(12), P. 2062 - 2062

Published: Nov. 30, 2023

The gas molecules O2, NO, H2S, CO, and CH4, have been increasingly used for medical purposes. Other than these molecules, H2 is the smallest diatomic molecule in nature has become a rising star medicine past few decades. As non-toxic easily accessible gas, shown preventive therapeutic effects on various diseases of respiratory, cardiovascular, central nervous system, other systems, but mechanisms are still unclear even controversial, especially mechanism as selective radical scavenger. Mitochondria main organelles regulating energy metabolism living organisms well organelle reactive oxygen species' generation targeting. We propose that protective role may be mainly dependent its unique ability to penetrate every aspect cells regulate mitochondrial homeostasis by activating Keap1-Nrf2 phase II antioxidant system rather direct free scavenging activity. In this review, we summarize focus mitochondria-targeting nutrient different disease models. addition, wish provide more rational theoretical support applications hydrogen.

Language: Английский

Citations

11
Molecular hydrogen attenuates sepsis-induced cardiomyopathy in mice by promoting autophagy DOI Creative Commons
Yan Cui,

Yingning Li,

Shuqi Meng

et al.

BMC Anesthesiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Feb. 23, 2024

Abstract Background Approximately 40 to 60% of patients with sepsis develop sepsis-induced cardiomyopathy (SIC), which is associated a substantial increase in mortality. We have found that molecular hydrogen (H 2 ) inhalation improved the survival rate and cardiac injury septic mice. However, mechanism remains unclear. This study aimed explore regulatory by modulates autophagy its role protection SIC. Methods Cecal ligation puncture (CLP) was used induce adult C57BL/6J male The mice were randomly divided into 4 groups: Sham, Sham + 2% ), CLP, CLP H group. 7-day recorded. Myocardial pathological scores calculated. troponin I (cTnI) levels serum detected, autophagy- mitophagy-related proteins myocardial tissue measured. Another four groups also studied: Bafilomycin A1 (BafA1), , BafA1 Mice group received an intraperitoneal injection inhibitor 1 mg/kg h after operation. detection indicators remained same as before. Results treated significantly improved, inflammation cTnI level decreased, flux increased, mitophagy protein content decreased ( P < 0.05). Compared group, showed decrease rate, level, reversed protective effect Conclusion Hydrogen exerts against SIC, may be achieved through promotion mitophagy.

Language: Английский

Citations

4