Intelligent salivary biosensors for periodontitis: in vitro simulation of oral oxidative stress conditions

Medical & Biological Engineering & Computing, Journal Year: 2024, Volume and Issue: 62(8), P. 2409 - 2434

Published: April 13, 2024

Language: Английский

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Saliva as a Diagnostic Tool for Early Detection of Exercise-Induced Oxidative Damage in Female Athletes

Biomedicines, Journal Year: 2024, Volume and Issue: 12(5), P. 1006 - 1006

Published: May 2, 2024

Although blood still remains the most commonly utilized medium to detect increased levels of oxidative damage induced by exercise, saliva diagnostics have gained increasing popularity due their non-invasive nature and athlete-friendly collection process. Given that contribution various phases menstrual cycle may differ, aim this study was evaluate an agreement between salivary plasmatic lipid peroxidation products in female swimmers both follicular (F) luteal (L) at rest following exercise. Twelve well-trained aged 19.6 ± 1.1 years old were examined. We measured diene conjugates (DCs), triene (TCs), Schiff bases (SBs) lipids immediately after extraction from plasma. All studied two times each, different one cycle, before high-intensity interval exercise (HIIE). Salivary DCs, TCs, SBs significantly post-exercise compared pre-exercise, F L phases. A high positive correlation observed concentrations plasma participants phases, HIIE. Ordinary least regression analysis indicates there no proportional differential bias data. The Bland-Altman method also declares bias, since line equality within 95% confidence mean difference swimmers, There plots. Thus, is first report a quantifications

Language: Английский

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Salivary Oxidative-Antioxidant Profile Following Adjunctive Gaseous Ozone Administration in Non-Surgical Periodontal Treatment: A Randomized Controlled Trial

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(17), P. 5272 - 5272

Published: Sept. 5, 2024

Background: Periodontitis is associated with increased oxidative stress, which may impair treatment outcomes. Ozone therapy has shown promise in reducing stress and improving periodontal health. This study examined the impact of adjunctive gaseous ozone administration on salivary markers patients periodontitis stages II–IV grades A–C undergoing non-surgical (NSPT). Methods: Ninety were randomly allocated to either test group (NSPT administration) or control alone) using computer-generated randomization. The OzoneDTA system was used deliver at 2100 ppm for 60 s per site once weekly 4 weeks. Clinical parameters (probing depth [PD], clinical attachment level [CAL], plaque index [PI], gingival [GI]) (malondialdehyde [MDA], total antioxidant capacity [TAC], superoxide dismutase [SOD]) assessed by blinded examiners baseline, 3, 6 months post-treatment. Results: Mixed ANOVA revealed significant three-way interactions between time, treatment, stage grade biochemical measures (p < 0.001). exhibited improvements TAC (mean difference: 0.45 ± 0.12 mmol/L, p = 0.002), MDA (−0.38 0.09 nmol/mL, 0.001), SOD (65 18 U/mL, 0.001) compared group, more pronounced effects III IV. Large effect sizes (Cohen’s d > 0.8) observed baseline all markers. Conclusions: Gaseous as an adjunct NSPT can effectively improve markers, particularly IV periodontitis. enhanced outcomes be attributed ozone’s antimicrobial immunomodulatory properties, synergistically reduce promote healing.

Language: Английский

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Salivary cytokine profile in patients with ischemic stroke

Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)

Published: Aug. 25, 2021

Inflammation plays a crucial role in stroke pathogenesis. Thus, it is not surprising that cytokines, chemokines, and growth factors have been advocated diagnostics. Our study the first to evaluate salivary cytokine profile patients with ischemic stroke. Twenty-five subacute an age-, sex-, oral hygiene status-matched control group were enrolled study. The number of was set priori based on our previous experiment (α = 0.05, test power 0.9). Salivary concentrations tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), 10 (IL-10) assessed using ELISA method. We showed TNF-α IL-6 significantly higher, whereas IL-10 content statistically lower both non-stimulated (NWS) stimulated (SWS) whole saliva patients. However, evaluation cytokines NWS rather than SWS may be greater diagnostic value. Of particular note TNF-α, which indicate cognitive/physical impairment post-stroke individuals. This parameter distinguishes from healthy controls correlates cognitive decline severity functional impairment. It also differentiates (with high sensitivity specificity) normal cognition mild moderate Saliva alternative blood for assessing patients, although further studies larger patient population are needed.

Language: Английский

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Malondialdehyde (MDA) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) in ischemic stroke: a systematic review

The Egyptian Journal of Neurology Psychiatry and Neurosurgery, Journal Year: 2023, Volume and Issue: 59(1)

Published: June 30, 2023

Abstract Introduction The low blood flow in ischemic stroke reduces oxygen and glucose induces a series of reactions that produce free radicals. Free radicals can destroy cell membranes (lipid peroxidation) marked by the elevation malondialdehyde (MDA), damage deoxyribonucleic acid (DNA) showed 8-hydroxy-2’-deoxyguanosine (8-OHdG). This review aimed to assess conclude research-based study systematically analyze relationship MDA/8-OHdG stroke. Method Cochrane handbook for systematic reviews, guideline preferred reporting items meta-analysis (PRISMA), synthesis without (SWiM) reviews: was used as this review. Inclusion criteria were primary studies every design, articles published English around January 2000–December 2021, human subject. A literature search applied 15 electronic medical journals. authors assessed quality risk bias each included study. Results evaluated 374 found searching, 30 met review, then underwent assessment bias. Conclusion MDA has role oxidative stress biomarker, outcome predictor, mortality post-stroke cognitive impairment depression hand grip strength predictor stroke; while 8-OHdG

Language: Английский

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Could inflammation contribute to salivary gland dysfunction in patients with chronic heart failure?

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 10, 2022

Heart failure (HF) is one of the leading causes death worldwide. HF results not only in cardiovascular dysfunction, but also numerous pathologies oral cavity and salivary glands. The present study first to evaluate whether inflammatory anti-inflammatory factors may be related with occurrence hyposalivation patients. We evaluated potential biomarkers diagnostics HF. included 30 women sex- age-matched healthy controls. demonstrated significantly higher levels pro-inflammatory cytokines, Th1, Th2, Th17, chemokines growth unstimulated saliva patients compared However, do indicate dominance either branch immune response. concentration selected gland dysfunction normal secretion subjects (IL-1β, TNF-α, IL-7, IL-13, INF-γ, IL-12, IL-15, IL-5, IL-6, IL-9, IL-17, MCP-1/CCL-2, EOTAXIN/CCL11, RANTES/CCL5, GM-CSF, VEGF, FGF basic, PDFG-BB). Multivariate regression analysis showed that content highly dependent on function, i.e. flow rate, total protein amylase activity. Using receiver operating characteristic (ROC) analysis, we IL-12 EOTAXIN/CCL11 differentiated highest sensitivity specificity Interestingly, some pro- mediators exceeds their plasma. In addition, biomarker reflect plasma content, which suggest a different nature/severity changes at central (blood) local (salivary) levels. Although our was purely observational, parameters plasma, as well lack saliva-blood correlation, increased production/secretion these compounds cells ROC did confirm diagnostic utility cytokines differential diagnosis

Language: Английский

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NOX2 as a Biomarker of Academic Performance: Evidence from University Students during Examination

Antioxidants, Journal Year: 2024, Volume and Issue: 13(5), P. 551 - 551

Published: April 30, 2024

Background: Cortisol levels, oxidative stress, and lower cerebral performance seem to be closely related. This study aimed evaluate the question of whether exam stress affected endothelial function parameters in salivary samples students. Methods: A total 114 healthy students were recruited. All subjected a 21-item DASS questionnaire assess perceived stress. biomarkers evaluated at T0, during semester, T1, morning before exam, saliva samples. In vitro, HUVECs stimulated with cortisol, evaluated. Results: At cortisol levels significantly increased compared semester. Moreover, results correlated inversely score T1. addition, NOX2, H2O2 endothelin-1 increased, while NO bioavailability decreased. treatment human determined increase decrease function, association impaired eNOS phosphorylation. Conclusion: NOX2-mediated is mechanism that could mediate cortisol-induced transient dysfunction academic examination. Therefore, strategies monitor or modulate help reduce impact examination-related

Language: Английский

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“The effects of non-surgical periodontal treatment plus zinc and magnesium supplementation on oxidative stress and antioxidants enzymes in type 2 diabetes patients: a quasi-experimental study”

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: Aug. 4, 2024

Periodontal Disease (PD) associated with Type 2 Diabetes Mellitus (T2DM) is a chronic condition that affects the oral cavity of people living T2DM. The mechanisms interaction between type and diseases are complex involve multiple pathophysiological pathways related to systemic inflammatory process oxidative stress. Non-surgical periodontal treatment (NSTP) considered standard for management this disease; however, patients conditions such as do not seem respond adequately. For reason, use complementary treatments has been suggested support non-surgical reduce clinical consequences disease improve patient. zinc gluconate magnesium oxide an adjunct its effects on features stress in -type poorly understood.

Language: Английский

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Total Antioxidant Status in Critically Ill Patients with Traumatic Brain Injury and Secondary Organ Failure—A Systematic Review

Diagnostics, Journal Year: 2024, Volume and Issue: 14(22), P. 2561 - 2561

Published: Nov. 15, 2024

The available literature indicates that oxidant-antioxidant imbalance plays a significant role in the pathophysiology of traumatic brain injury and subsequent secondary organ dysfunctions. However, there is lack studies summarizing knowledge this area, no clear guidelines exist regarding use biomarkers oxidative stress as diagnostics tools.

Language: Английский

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Application of a Novel Biosensor for Salivary Conductivity in Detecting Chronic Kidney Disease

Biosensors, Journal Year: 2022, Volume and Issue: 12(3), P. 178 - 178

Published: March 17, 2022

The prevalence of chronic kidney disease (CKD) is increasing, and it brings an enormous healthcare burden. traditional measurement function needs invasive blood tests, which hinders the early detection causes low awareness CKD. We recently designed a device with miniaturized coplanar biosensing probes for measuring salivary conductivity at extremely volume (50 μL). Our preliminary data discovered that was significantly higher in CKD patients. This cross-sectional study aims to validate relationship between function, represented by estimated glomerular filtration rate (eGFR). enrolled 214 adult participants mean age 63.96 ± 13.53 years, whom 33.2% were male. CKD, defined as eGFR < 60 mL/min/1.73 m2, 11.2% our study. By multivariate linear regression analyses, we found positively related fasting glucose but negatively associated eGFR. further divided subjects into low, medium, high groups according tertials levels. There significant trend increment patients from (4.2% vs. 12.5% 16.9%, p trend: 0.016). receiver operating characteristic (ROC) curves disclosed excellent performance using combined age, gender, body weight diagnose (AUC equal 0.8). adjusted odds ratio 2.66 (95% CI, 1.10–6.46) Overall, can serve good surrogate marker function; this real-time, non-invasive, easy-to-use portable may be reliable tool screening

Language: Английский

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