[Mechanism of extracellular vesicles in the repair of intervertebral disc degeneration]. DOI
S.Y. Zhu, Zhonghai Li

PubMed, Journal Year: 2025, Volume and Issue: 42(2), P. 409 - 416

Published: April 25, 2025

Extracellular vesicles (EVs), defined as cell-secreted nanoscale that carry bioactive molecules, have emerged a promising therapeutic strategy in tumor and tissue regeneration. Their potential repairing intervertebral disc degeneration (IDD) through multidimensional regulatory mechanisms is rapidly advancing field of research. This paper provided an overview the EVs IDD repair, thoroughly reviewed recent literature on for IDD, domestically internationally, summarized their mechanisms. In could act different at molecular, cellular, levels. At molecular level, treat by inhibiting inflammatory reactions, suppressing oxidative stress, regulating synthesis decomposition extracellular matrix. cellular pyroptosis, ferroptosis, apoptosis promoting cell proliferation differentiation. neovascularization. strong clinical application treatment deserve more profound study.

Language: Английский

Polydopamine Nanoparticles Targeting Ferroptosis Mitigate Intervertebral Disc Degeneration Via Reactive Oxygen Species Depletion, Iron Ions Chelation, and GPX4 Ubiquitination Suppression DOI Creative Commons
Xiao Yang, Yan Chen,

Jiadong Guo

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(13)

Published: March 23, 2023

Intervertebral disc degeneration (IVDD)-induced lower back pain (LBP) is a common problem worldwide. The underlying mechanism partially accredited to ferroptosis, based on sequencing analyses of IVDD patients from the gene expression omnibus (GEO) databases. In this study, it shown that polydopamine nanoparticles (PDA NPs) inhibit oxidative stress-induced ferroptosis in nucleus pulposus (NP) cells vitro. PDA NPs scavenge reactive oxygen species (ROS), chelate Fe

Language: Английский

Citations

91
Apoptotic Bodies Derived from Fibroblast‐Like Cells in Subcutaneous Connective Tissue Inhibit Ferroptosis in Ischaemic Flaps via the miR‐339‐5p/KEAP1/Nrf2 Axis DOI Creative Commons

Gaoxiang Yu,

Yijie Chen, Ningning Yang

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(24)

Published: April 19, 2024

Abstract Preventing and treating avascular necrosis at the distal end of flaps are critical to surgery success, but current treatments not ideal. A recent study shows that apoptotic bodies (ABs) generated near site apoptosis can be taken up promote cell proliferation. The reveals ABs derived from fibroblast‐like cells in subcutaneous connective tissue (FSCT cells) skin promoted ischaemic flap survival. It is also found inhibited death oxidative stress M1‐to‐M2 polarization macrophages. Transcriptome sequencing protein level testing demonstrated survival endothelial macrophages by inhibiting ferroptosis via KEAP1‐Nrf2 axis. Furthermore, microRNA (miR) data vitro vivo experiments KEAP1 delivering miR‐339‐5p exert therapeutic effects. In conclusion, FSCT cell‐derived ferroptosis, macrophage transition miR‐339‐5p/KEAP1/Nrf2 axis These results provide a potential strategy administering ABs.

Language: Английский

Citations

20
Application of mesenchymal stem cell-derived exosomes from different sources in intervertebral disc degeneration DOI Creative Commons

Yuanliang Xia,

Ruohan Yang,

Yulin Hou

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: Oct. 7, 2022

Intervertebral disc degeneration (IVDD) is a main cause of lower back pain, leading to psychological and economic burdens patients. Physical therapy only delays pain in patients but cannot eliminate the IVDD. Surgery required when patient tolerate or has severe neurological symptoms. Although surgical resection IVD decompression laminae eliminates diseased segment, it damages adjacent normal IVD. There also risk re-protrusion after removal. Cell played crucial role development regenerative medicine. transplantation promotes regeneration degenerative tissue. However, owing lack vascular structure IVD, sufficient nutrients be provided for transplanted mesenchymal stem cells (MSCs). In addition, dead release harmful substances that aggravate Extracellular vesicles (EVs) have been extensively studied as an emerging therapeutic approach. EVs generated by paracrine MSCs retain potential serve carriers deliver their contents target regulate cell activity. Owing double-layered membrane structure, low immunogenicity no immune rejection. Therefore, are considered modality they limited mass production loading rates. this review, advantages introduced, application MSC-EVs IVDD discussed. The current limitations future applications described.

Language: Английский

Citations

40
The mechanisms and functions of IL-1β in intervertebral disc degeneration DOI Creative Commons
Hongtao Li, Xiaoqiang Wang, Hongyu Pan

et al.

Experimental Gerontology, Journal Year: 2023, Volume and Issue: 177, P. 112181 - 112181

Published: April 24, 2023

Intervertebral disc degeneration (IDD) is the leading cause of low back pain (LBP) and disability in elderly, imposing significant public health economic burden worldwide. Meanwhile, pathological mechanisms IDD remain complicated, treatment strategy to reverse primarily due unclear specific lack particular effective targets. Interleukin-1β (IL-1β), one most important members IL-1 family, can induce solid pro-inflammatory activity by stimulating secretion various mediators considered key mediator. However, recent years, IL-1β be able regulate IVD cell death many ways, such as apoptosis, pyroptosis, ferroptosis, so on. At same time, numerous studies on inhibitors suggest that inhibition may a promising biological therapy for IDD. Many have been investigated through pathogenic mechanisms, including inhibiting inflammatory processes, regulating ECM degradation, more. Therefore, anti-IL-1β effect alleviating degeneration. This article mainly reviews functions investigates advances biotherapeutic approach

Language: Английский

Citations

33
The role of ferroptosis in intervertebral disc degeneration DOI Creative Commons

Chun‐Yang Fan,

Genglei Chu, Zilin Yu

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: July 27, 2023

Nucleus pulposus, annulus fibrosus, and cartilage endplate constitute an avascular intervertebral disc (IVD), which is crucial for spinal joint mobility. As one of the most widespread health issues worldwide, degeneration (IVDD) recognized as a key contributor to back neck discomfort. A number degenerative disorders have strong correlation with ferroptosis, recently identified novel regulated cell death (RCD) characterized by iron-dependent mechanism buildup lipid reactive oxygen species (ROS). There growing interest in part ferroptosis plays IVDD pathophysiology. Inhibiting has been shown control development. Several studies demonstrated that TBHP-induced oxidative stress models, changes marker protein levels increased peroxidation lead cells, subsequently aggravates IVDD. Similarly, significantly relieved use inhibitors. The purpose this review was threefold: 1) discuss occurrence IVDD; 2) understand its role pathophysiology; 3) investigate feasibility prospect treatment.

Language: Английский

Citations

31
MicroRNA-targeting nanomedicines for the treatment of intervertebral disc degeneration DOI Creative Commons
Hussein H. Genedy, P. Humbert,

Bilel Laoulaou

et al.

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 207, P. 115214 - 115214

Published: Feb. 22, 2024

Language: Английский

Citations

13
Mesenchymal stem cells and ferroptosis: Clinical opportunities and challenges DOI Creative Commons

Mengling Cui,

Fukun Chen,

Lishi Shao

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(3), P. e25251 - e25251

Published: Feb. 1, 2024

This review discusses recent experimental and clinical findings related to ferroptosis, with a focus on the role of MSCs. Therapeutic efficacy current applications MSC-based ferroptosis therapies are also discussed.

Language: Английский

Citations

11
Nrf2 activation: a key mechanism in stem cell exosomes-mediated therapies DOI Creative Commons
Zeinab Vahidinia,

Abolfazl Azami Tameh,

Shirin Barati

et al.

Cellular & Molecular Biology Letters, Journal Year: 2024, Volume and Issue: 29(1)

Published: March 2, 2024

Abstract Exosomes are nano-sized membrane extracellular vesicles which can be released from various types of cells. originating inflammatory or injured cells have detrimental effects on recipient cells, while exosomes derived stem not only facilitate the repair and regeneration damaged tissues but also inhibit inflammation provide protective against diseases, suggesting they may serve as an alternative strategy transplantation. a fundamental role in communication between through transfer proteins, bioactive lipids nucleic acids (like miRNAs mRNAs) This significantly impacts both physiological pathological functions Nuclear factor erythroid 2–related 2 (Nrf2), transcription factor, is able to mitigate damage caused by oxidative stress signaling pathways. The positive resulting activation Nrf2 pathway different disorders been documented literature. Studies confirmed that could act effective agonists. However, limited studies explored therapeutic cell-derived exosomes. review provides comprehensive overview existing knowledge concerning pathways impact exerted cell some common diseases. Graphical

Language: Английский

Citations

11
Ferroptosis: A potential target for the intervention of intervertebral disc degeneration DOI Creative Commons
Lu-Ping Zhou, Renjie Zhang, Chong-Yu Jia

et al.

Frontiers in Endocrinology, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 20, 2022

Ferroptosis, an iron-dependent form of programmed cell death marked by phospholipid peroxidation, is regulated complex cellular metabolic pathways including lipid metabolism, iron balance, redox homeostasis, and mitochondrial activity. Initial research regarding the mechanism ferroptosis mainly focused on solute carrier family 7 member 11/glutathione/glutathione peroxidase 4 (GPX4) signal pathway. Recently, novel mechanisms ferroptosis, independent GPX4, have been discovered. Numerous pathologies associated with extensive such as drug-resistant cancers, ischemic organ injuries, neurodegenerative diseases, are driven ferroptosis. Ferroptosis a new therapeutic target for intervention IVDD. The role in modulation intervertebral disc degeneration (IVDD) significant topic interest. This topic, IVDD ongoing. Herein, we aim to review discuss literature explore relationship between regulatory networks cells nucleus pulposus, annulus fibrosus, cartilage endplate provide references future basic clinical translation treatment.

Language: Английский

Citations

33
Regulation of the Nrf2/HO-1 axis by mesenchymal stem cells-derived extracellular vesicles: implications for disease treatment DOI Creative Commons

Hua Su,

Zhongan Wang,

Lidan Zhou

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: June 10, 2024

This comprehensive review inspects the therapeutic potential of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) across multiple organ systems. Examining their impact on integumentary, respiratory, cardiovascular, urinary, and skeletal systems, study highlights versatility MSC-EVs in addressing diverse medical conditions. Key pathways, such as Nrf2/HO-1, consistently emerge central mediators antioxidative anti-inflammatory effects. From expediting diabetic wound healing to mitigating oxidative stress-induced skin injuries, alleviating acute lung even offering solutions for conditions like myocardial infarction renal ischemia-reperfusion injury, demonstrate promising efficacy. Their adaptability different administration routes identifying specific factors opens avenues innovative regenerative strategies. positions candidates future clinical applications, providing a overview medicine.

Language: Английский

Citations

8