Lactate metabolism in human health and disease

Signal Transduction and Targeted Therapy, Journal Year: 2022, Volume and Issue: 7(1)

Published: Sept. 1, 2022

Abstract The current understanding of lactate extends from its origins as a byproduct glycolysis to role in tumor metabolism, identified by studies on the Warburg effect. shuttle hypothesis suggests that plays an important bridging signaling molecule coordinates among different cells, organs and tissues. Lactylation is posttranslational modification initially reported Professor Yingming Zhao’s research group 2019. Subsequent confirmed lactylation vital component function involved proliferation, neural excitation, inflammation other biological processes. An indispensable substance for various physiological cellular functions, regulatory aspects energy metabolism signal transduction. Therefore, comprehensive review summary presented clarify disease provide reference direction future research. This offers systematic overview homeostasis roles pathological processes, well effects diseases, particularly cancer.

Language: Английский

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CRISPR/Cas9 therapeutics: progress and prospects

Signal Transduction and Targeted Therapy, Journal Year: 2023, Volume and Issue: 8(1)

Published: Jan. 16, 2023

Abstract Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene-editing technology is the ideal tool of future for treating diseases by permanently correcting deleterious base mutations or disrupting disease-causing genes with great precision and efficiency. A variety efficient Cas9 variants derivatives have been developed to cope complex genomic changes that occur during diseases. However, strategies effectively deliver CRISPR system diseased cells in vivo are currently lacking, nonviral vectors target recognition functions may be focus research. Pathological physiological resulting from disease onset expected serve as identifying factors targeted delivery targets gene editing. Diseases both varied complex, choice appropriate methods different important. Meanwhile, there still many potential challenges identified when targeting CRISPR/Cas9 treatment. This paper reviews current developments three aspects, namely, type, vector, characteristics. Additionally, this summarizes successful examples clinical trials finally describes possible problems associated applications.

Language: Английский

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Recent advances in targeted delivery of non-coding RNA-based therapeutics for atherosclerosis

Molecular Therapy, Journal Year: 2022, Volume and Issue: 30(10), P. 3118 - 3132

Published: Aug. 2, 2022

Cardiovascular disease (CVD) has overtaken infectious illnesses as the leading cause of mortality and disability worldwide. The pathology that underpins CVD is atherosclerosis, characterized by chronic inflammation caused accumulation plaques in arteries. As our knowledge about microenvironment blood vessel walls deepens, there an opportunity to fine-tune treatments target mechanisms driving atherosclerosis more directly. application non-coding RNAs (ncRNAs) biomarkers or intervention targets increasing. Although these ncRNAs play important role vascular dysfunction, cellular extracellular environments pose a challenge for targeted transmission therapeutic regulation ncRNAs. Specificity, delivery, tolerance have hampered clinical translation ncRNA-based therapeutics. Nanomedicine emerging field uses nanotechnology drug delivery advanced imaging. Recently, nanoscale carriers shown promising results introduced new possibilities nucleic acid particularly atherosclerosis. In this review, we discuss latest developments nanoparticles aid development, miRNA, analyze current challenges ncRNA delivery. particular, highlight emergence various kinds nanotherapeutic approaches based on ncRNAs, which can improve treatment options

Language: Английский

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Lysine Malonylation and Its Links to Metabolism and Diseases

Aging and Disease, Journal Year: 2022, Volume and Issue: 14(1), P. 84 - 84

Published: Nov. 17, 2022

Malonylation is a recently identified post-translational modification with malonyl-coenzyme A as the donor. It conserved both in prokaryotes and eukaryotes. Recent advances identification quantification of lysine malonylation by bioinformatic analysis have improved our understanding its role regulation protein activity, interaction, localization elucidated involvement many biological processes. has been linked to diverse physiological processes, including metabolic disorders, inflammation, immune regulation. This review discusses theory, describes underlying mechanism, summarizes recent progress research. The latest findings point novel functions highlight mechanisms which regulates variety cellular Our also marks association between malonylation, enzymes involved, various diseases, promising diagnostic therapeutic biomolecular targets for future clinical applications.

Language: Английский

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piRNA-823 is a novel potential therapeutic target in aortic dissection

Pharmacological Research, Journal Year: 2023, Volume and Issue: 196, P. 106932 - 106932

Published: Sept. 20, 2023

Aortic dissection (AD) presents a medical challenge for clinicians. Here, to determine the role of novel small non-coding piRNA-823 (piR-823) in AD, murine and human aorta from patients with AD were used. A high expression levels piR-823 found AD. Using performed loss- gain-of-function assays vitro vivo, we explore regulatory effect on vascular smooth muscle cells (VSMCs) obviously facilitates proliferation, migration, phenotypic transformation VSMCs or without nicotine treatment. directly binds suppresses histone deacetylase 1 (HDAC1) expression, regulates acetylation 3 (H3) via H3K9ac H3K27ac, eventually, VSMC functions To consolidate our findings, model was performed, observed that antagomir strongly inhibited pathogenesis through regulating remodeling. Thus, study finds potential target prevention treatment strategy nicotine-induced

Language: Английский

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Targeting mitochondrial dynamics and redox regulation in cardiovascular diseases

Trends in Pharmacological Sciences, Journal Year: 2024, Volume and Issue: 45(4), P. 290 - 303

Published: March 7, 2024

Language: Английский

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Protective effect and mechanism of ginsenoside Rg2 on atherosclerosis

Journal of Ginseng Research, Journal Year: 2022, Volume and Issue: 47(2), P. 237 - 245

Published: Aug. 6, 2022

Ginsenoside Rg2 (Rg2) has a variety of pharmacological activities and provides benefits during inflammation, cancer, other diseases. However, there are no reports about the relationship between atherosclerosis.We used 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to detect cell viability in vascular smooth muscle cells (VSMCs) human umbilical vein endothelial (HUVECs). The expression inflammatory factors HUVECs phenotypic transformation-related marker VSMCs were detected at mRNA levels. Western blot method was inflammation pathways transformation protein rat carotid balloon injury model performed explore effect on vivo.Rg2 decreased induced by lipopolysaccharide HUVECs-without affecting viability. These events depend blocking regulation NF-κB p-ERK signaling pathway. In VSMCs, can inhibit proliferation, migration, platelet derived growth factor-BB (PDGF-BB)-which may contribute its anti-atherosclerotic role. rats with injury, reduce intimal proliferation after regulate pathway response, also suppress VSMCs.These results suggest that exert cellular level animal level, which more sufficient basis for ginseng as functional dietary regulator.

Language: Английский

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miR-564: A potential regulator of vascular smooth muscle cells and therapeutic target for aortic dissection

Journal of Molecular and Cellular Cardiology, Journal Year: 2022, Volume and Issue: 170, P. 100 - 114

Published: June 18, 2022

Language: Английский

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The lncRNA Punisher Regulates Apoptosis and Mitochondrial Homeostasis of Vascular Smooth Muscle Cells via Targeting miR-664a-5p and OPA1

Oxidative Medicine and Cellular Longevity, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 21

Published: May 25, 2022

Long noncoding RNAs (lncRNAs) are important regulators of various cellular functions. Recent studies have shown that a novel lncRNA termed Punisher is highly expressed in cardiovascular progenitors and has potential role diseases. However, its role, especially molecular mechanism, unclear. In our present study, we observed was obviously downregulated atherosclerotic plaques. Further research proved it can suppress the apoptosis VSMCs potentially contributing to progression atherosclerosis. Intriguingly, revealed regulate mitochondria fission as well mitochondrial functions induced by hydrogen peroxide (H2O2) VSMCs. Mechanistically, further serve ceRNA which directly binds miR-664a-5p consequently regulates target OPA1, finally contributes biological function Particularly, overexpression distinctly suppressed neointima formation VSMC vivo. Encouragingly, these results were accordance with findings obtained clinical evaluation patients Our data provides significant relationship among homeostasis, apoptosis, And could targets diagnosis treatment

Language: Английский

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Importance of long non-coding RNAs in the pathogenesis, diagnosis, and treatment of myocardial infarction

IJC Heart & Vasculature, Journal Year: 2024, Volume and Issue: 55, P. 101529 - 101529

Published: Oct. 17, 2024

Language: Английский

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