Journal of Clinical Medicine,
Journal Year:
2024,
Volume and Issue:
13(23), P. 7159 - 7159
Published: Nov. 26, 2024
Bladder
cancer
(BC)
currently
ranks
as
the
9th
most
common
worldwide.
It
is
characterised
by
very
high
rates
of
recurrence
and
metastasis.
Most
cases
BC
are
urothelial
origin,
due
to
its
ability
penetrate
muscle
tissue,
divided
into
non-muscle-invasive
(NMIBC)
muscle-invasive
(MIBC).
The
current
diagnosis
still
based
primarily
on
invasive
cystoscopy,
which
an
expensive
method
that
carries
a
risk
various
complications.
Urine
sediment
cytology
often
used
complementary
test,
biggest
drawback
low
sensitivity
concerning
detection
at
early
stages,
crucial
for
prompt
implementation
appropriate
treatment.
Therefore,
there
great
need
develop
innovative
diagnostic
techniques
would
enable
accurate
prognosis
BC.
Great
potential
in
this
regard
shown
epigenetic
changes,
possible
observe
long
before
onset
clinical
symptoms
disease.
In
addition,
these
changes
can
be
detected
readily
available
biological
material,
such
urine
or
blood,
indicating
possibility
constructing
non-invasive
tests.
Over
past
few
years,
many
studies
have
emerged
using
alterations
novel
prognostic
biomarkers
This
review
provides
update
promising
DNA
methylation
expression
levels
selected
non-coding
RNAs
(ncRNAs),
taking
account
latest
literature
data.
Non-coding RNA Research,
Journal Year:
2023,
Volume and Issue:
8(3), P. 302 - 314
Published: March 21, 2023
Long
non-coding
RNA
(LncRNA)
telomerase
component
(TERC)
has
telomerase-dependent
and
independent
activity
in
numerous
cancer
types.
The
present
study
purposes
to
demonstrate
the
role
of
lncRNA
TERC
as
a
diagnostic
serum
biomarker
colorectal
(CRC)
patients
molecular
mechanism
inducing
tumor
CRC
cell
lines.PCR
array
was
performed
examine
lncRNAs
dysregulated
CRC.
LncRNA
expression
level
evaluated
70
35
control
subjects
using
RT-qPCR.
Then
transfection
build
down-expression
models
TERC.
ROC
curve
analysis
applied
assess
value
In
addition,
RT-qPCR
used
detect
β-catenin
mRNA.
Moreover,
ELISA
Western
blot
were
protein
sera
lines.
biological
functions
such
growth
migration
cells
assessed
wound
healing
assay.
Cell
cycle
apoptosis
flow
cytometry.The
is
overexpressed
with
high
stage
discrimination
accuracy.
Furthermore,
upregulated
lines
silencing
induced
arrest
inhibited
migration.
inhibition
reduces
levels.The
could
be
considered
an
early
stages
good
ability
discriminate
between
stages.
induces
by
promoting
evading
elevating
protein.
International Journal of Molecular Sciences,
Journal Year:
2022,
Volume and Issue:
23(5), P. 2461 - 2461
Published: Feb. 23, 2022
Exosomes
are
a
subset
of
nano-sized
extracellular
vesicles
originating
from
endosomes.
mediate
cell-to-cell
communication
with
their
cargos,
which
includes
mRNAs,
miRNAs,
lncRNAs,
and
circRNAs.
Exosomal
RNAs
have
cell
specificity
reflect
the
conditions
donor
cells.
Notably,
detection
in
biofluids
can
be
used
as
diagnostic
marker
for
various
diseases.
ideal
biomarkers
because
surrounding
membranes
confer
stability
they
detectable
almost
all
biofluids,
helps
to
reduce
trauma
avoid
invasive
examinations.
However,
knowledge
exosomal
remains
scarce.
The
present
review
summarizes
biogenesis,
secretion,
uptake
exosomes,
current
researches
exploring
circRNAs
potential
diagnosis
human
diseases,
well
recent
techniques
exosome
isolation.
International Journal of Molecular Sciences,
Journal Year:
2022,
Volume and Issue:
23(15), P. 8597 - 8597
Published: Aug. 2, 2022
The
non-muscle
invasive
bladder
cancer
tends
to
recur
and
progress.
Therefore,
it
requires
frequent
follow-ups,
generating
costs
making
one
of
the
most
expensive
neoplasms.
Considering
character
current
gold-standard
diagnostic
procedure,
white-light
cystoscopy,
efforts
find
an
alternative
method
are
ongoing.
Although
last
decade
has
seen
significant
advancements
in
urinary
biomarker
tests
(UBTs)
for
cancer,
international
guidelines
have
not
recommended
them.
Currently,
paramount
urgency
is
validate
test
with
best
specificity
sensitivity,
which
would
allow
optimizing
diagnosis,
prognosis,
a
treatment
plan.
This
review
aims
summarise
up-to-date
state
knowledge
relating
UBTs
new
developments
detection,
surveillance
their
potential
applications
clinical
practice.
The International Journal of Biological Markers,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 13, 2025
Purpose
We
aimed
to
exploit
a
urine
exosomal
long
non-coding
RNAs
(lncRNAs)
fingerprint
facilitate
the
early
diagnosis
of
bladder
cancer.
Methods
Microarray
differential
expression
profiling
lncRNAs
was
for
first
time
employed
in
exosomes
from
10
non-muscle-invasive
cancer
(NMIBC)
patients
and
healthy
controls
screen
out
candidate
lncRNA
biomarkers,
which
were
then
verified
by
quantitative
real-time
polymerase
chain
reaction
three
independent
phases
including
cells,
culture
fluid
200
NMIBC
participants.
Logistic
regression
performed
construct
diagnostic
model—the
potency
assessed.
Results
The
profile
exosome-derived
(CCDC148-AS1,
XLOC_006419,
RP5-1148A21.3)
screened
further
be
notably
over-expressed
cell
lines,
exhibited
area
under
receiver-operating
characteristic
curve
values
0.873,
0.825,
0.834,
respectively,
training,
validation,
double-blind
validation
phases.
superior
urinary
cytology
discriminating
(
P
<
0.0001).
A
significant
correlation
existed
between
higher
level
CCDC148-AS1
tumor
grade
0.001),
up-regulated
as
well
XLOC_006419
statistically
related
with
node
metastasis
stage
=
0.004
0.031,
respectively).
These
identified
confirmed
originate
cells
packaged
within
exosomes,
thus
staying
sufficiently
stable
urine.
Conclusions
Tumor-originated
lncRNAs,
NMIBC,
satisfying
clinical
significance
Clinical & Translational Oncology,
Journal Year:
2024,
Volume and Issue:
26(6), P. 1497 - 1507
Published: Jan. 16, 2024
Abstract
Background
In
view
of
discordance
consisting
in
different
reports,
a
meta-analysis
was
conducted
to
comprehensively
evaluate
the
diagnostic
efficacy
exosomal
noncoding
RNAs
(ncRNAs)
blood
and
urine
detection
bladder
cancer.
Methods
Eligible
studies
were
acquired
by
systematic
retrieval
through
PubMed,
Cochrane
Library,
Embase.
The
pooled
appraised
reckoning
area
under
summary
receiver
operating
characteristic
(SROC)
curve.
latent
sources
heterogeneity
probed
subgroup
analyses
meta-regression.
STATA
12.0,
Meta-DiSc
1.4,
RevMan
5.3
applied
carry
out
all
statistical
plots.
Results
A
total
46
from
15
articles
comprising
2622
controls
3015
cancer
patients
included
our
meta-analysis.
Exosomal
ncRNAs
represented
relatively
satisfactory
detecting
cancer,
with
sensitivity
0.75,
specificity
0.79,
an
SROC
curve
(AUC)
0.84.
microRNAs
(miRNAs)
exhibited
better
value
AUC
0.91
than
that
long
(lncRNAs).
To
some
extent,
among
induced
ncRNA
types
(miRNA
or
lncRNA),
profiling
(single-
multiple-ncRNA),
sample
size,
specimen
types,
ethnicity.
Conclusion
may
play
vital
role
diagnosing
as
prospective
noninvasive
biomarkers;
nonetheless,
their
clinical
performance
needs
be
confirmed
further
massive
proactive
researches.
Environment International,
Journal Year:
2024,
Volume and Issue:
184, P. 108462 - 108462
Published: Jan. 28, 2024
While
Alzheimer's
disease
(AD)
diagnosis,
management,
and
care
have
become
priorities
for
healthcare
providers
researcher's
worldwide
due
to
rapid
population
aging,
epidemiologic
surveillance
efforts
are
currently
limited
by
costly,
invasive
diagnostic
procedures,
particularly
in
low
middle
income
countries
(LMIC).
In
recent
years,
wastewater-based
epidemiology
(WBE)
has
emerged
as
a
promising
tool
public
health
assessment
through
detection
quantification
of
specific
biomarkers
wastewater,
but
applications
non-infectious
diseases
such
AD
remain
limited.
This
early
review
seeks
summarize
AD-related
urine
other
peripheral
biofluids
discuss
their
potential
integration
WBE
platforms
guide
the
first
prospective
field.
Promising
results
been
reported
clinical
settings,
indicating
amyloid
β,
tau,
neural
thread
protein,
long
non-coding
RNAs,
oxidative
stress
markers
dysregulated
metabolites
questions
regarding
concentration
stability
wastewater
correlation
between
levels
sewage
circulation
must
be
addressed
future
studies
before
comprehensive
systems
can
developed.
Biomedicine & Pharmacotherapy,
Journal Year:
2023,
Volume and Issue:
165, P. 115027 - 115027
Published: June 22, 2023
The
types
of
urothelial
carcinoma
(UC)
include
bladder
cancer
and
upper
tract
carcinoma.
Current
diagnostic
techniques
cannot
meet
the
needs
patients.
Liquid
biopsy
is
an
accurate
method
determining
molecular
profile
UC
a
cutting-edge
popular
technique
that
expected
to
complement
existing
detection
benefit
patients
with
UC.
Circulating
tumor
cells,
cell-free
DNA,
RNA,
extracellular
vesicles,
proteins,
metabolites
can
be
found
in
blood,
urine,
or
other
bodily
fluids
are
examined
during
liquid
biopsies.
This
article
focuses
on
components
biopsies
their
clinical
applications
have
tremendous
potential
multiple
aspects
precision
oncology,
from
early
diagnosis
treatment
monitoring
predicting
prognoses.
They
may
therefore
play
important
role
management
medicine.
Frontiers in Cell and Developmental Biology,
Journal Year:
2024,
Volume and Issue:
12
Published: Aug. 15, 2024
Liver
cancer
is
one
of
the
most
lethal
gastrointestinal
malignancies.
Emerging
evidence
has
underscored
pivotal
role
long
non-coding
RNAs
(lncRNAs)
in
tumorigenesis,
with
ST8SIA6-AS1
identified
as
a
novel
oncogenic
lncRNA
contributing
to
liver
progression.
consistently
upregulated
hepatic
tissues
and
strongly
associated
unfavorable
prognosis.
Moreover,
it
demonstrates
high
diagnostic
efficacy
detecting
HCC.
involved
various
cellular
processes
including
proliferation,
migration,
invasion,
primarily
through
its
function
competing
endogenous
RNA
(ceRNA),
thereby
facilitating
hepatocarcinogenesis
disease
advancement.
This
review
provides
detailed
examination
molecular
functions
regulatory
mechanisms
hepatocellular
carcinoma
(HCC)
highlights
potential
promising
biomarker
for
cancer,
aiming
propel
development
innovative
therapeutic
strategies
HCC
management.
Frontiers in Oncology,
Journal Year:
2022,
Volume and Issue:
12
Published: Sept. 20, 2022
Bladder
cancer
is
one
of
the
top
ten
most
common
cancers
and
causes
death
globally.
5-year
survival
rates
have
decreased
in
Australia
from
66%
to
55%
past
three
decades.
The
current
gold
standard
for
diagnosis
cystoscopy.
However,
cystoscopies
are
an
invasive
health-resource
intensive
procedure
which
has
sub-optimal
sensitivity
flat
lesions
such
as
CIS
(carcinoma
situ)
low
specificity
differentiating
inflammation
-
hence
requiring
biopsies
under
anesthesia.
Frequent
life-long
surveillance
cystoscopy
required
patients
since
there
high
progression
local
recurrence
high-risk
non-muscle
(NMIBC)
well
poor
outcomes
associated
with
delayed
detection
muscle-invasive
bladder
(MIBC).
There
unmet
need
a
non-invasive
test
provide
better
discrimination
risk-stratification
could
aid
clinicians
by
improving
patient
selection
cystoscopy;
enhanced
risk
stratification
methods
may
guide
frequency
inform
treatment
choices.
Exosomes,
nano-sized
extracellular
vesicles
containing
genetic
material
proteins,
been
shown
functional
roles
development
cancer.
Exosomes
also
demonstrated
be
robust
source
potential
biomarkers
prognosis
therapeutic
agents.
In
this
review,
we
summarize
latest
evidence
biological
exosomes
highlight
their
clinical
significance
diagnosis,
treatment.
