Carob seeds as a source of bioactive flavonoid derivatives: isolation, network pharmacology‐guided anti‐cancer activity, and HPLC standardization DOI
Asmaa M. Khalil, Nesrin M. Fayek, Omar M. Sabry

et al.

Chemistry & Biodiversity, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Abstract Carob, Ceratonia siliqua L. (CS), is a legume well‐known for its edible pod pulp. Its seeds are used almost exclusively as source of the food additive E410. Although variety metabolites have been identified by HPLC and LC–MS analysis in CS, reports concerned with their isolation scarce. In this study, two flavonoid derivatives were isolated from methanolic extract CS seeds, namely, quercetin‐3‐ O ‐rhamnoside 4′‐ p ‐hydroxybenzoylisorhamnetin‐3,7‐di‐ ‐rhamnoside. Network pharmacology was unusually guide estimation biological potential compounds. Finally, ethyl acetate fraction standardized content HPLC. The isolates displayed ability to interfere activity several target proteins associated renal colon cancers. Their cytotoxic effect on colorectal cancer cell lines investigated comparison Doxorubicin. selectivity compounds evaluated normal human fetal fibroblast lines. showed very potent against tested highest selectivity. can be bioactive that incorporated pharmaceutical industries.

Language: Английский

Harnessing polysaccharides for sustainable food packaging DOI Creative Commons

Ummugulsum Tukenmez Emre,

Seda Şirin,

Serap Nigdelioglu Dolanbay

et al.

Polymer Bulletin, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Language: Английский

Citations

2
Nutritional Benefits and Antihyperglycemic Potential of Carob Fruit (<i>Ceratonia siliqua</i> L.) - An Overview DOI Creative Commons
Salah Laaraj, Ashiq Hussain, Aziz Mouhaddach

et al.

Ecological Engineering & Environmental Technology, Journal Year: 2024, Volume and Issue: 25(3), P. 124 - 132

Published: Jan. 27, 2024

Carob (Ceratonia siliqua L.), a plant native to the Mediterranean, is member of legume family and its fruit called pod.The flesh pod (pulp) very rich in sugar, while seeds are high protein.The also good source minerals such as potassium, calcium phosphorus.It polyphenols antioxidants.Due nutritional composition, it suitable for improving human well-being.This article reviews chemical composition locust bean biological effects on health.Understanding traditional uses relation potential an anti-diabetic agent important view numerous recent scientific studies pharmacological properties.The study focuses vivo vitro antihyperglycemic research, well profile food applications this natural product formulation fortification.Based properties, species believed have beneficial preventive therapeutic effects, particularly hyperglycemia.Researchers can further extract isolate bioactive compounds from different carob fractions develop pharmaceutical products functional foods industries.

Language: Английский

Citations

5
Evaluation of carob tree (Ceratonia siliqua L.) pods, through three different drying techniques, and ultrasonic assisted extraction, for presence of bioactives DOI
Ashiq Hussain, Muhammad Arif, Adnan Ahmed

et al.

South African Journal of Botany, Journal Year: 2024, Volume and Issue: 173, P. 388 - 396

Published: Aug. 29, 2024

Language: Английский

Citations

4
Unveiling the potential applications of plant by-products in food – A review DOI Creative Commons
Macdalyna Esther Ronie, Ahmad Hazim Abdul Aziz, Kobun Rovina

et al.

Waste Management Bulletin, Journal Year: 2024, Volume and Issue: 2(3), P. 183 - 203

Published: July 27, 2024

In the pursuit of sustainable and innovative food production, utilisation plant by-products have emerged as a promising frontier. Plant an inherent value that can be utilised to address both environmental concerns growing demand for resources. This review provides critical impact caused by disposal waste are not being reutilised or repurposed benefit other products. The multifaceted applications agricultural residues, traditionally deemed waste, in creation diverse nutritionally enriched products, were also presented this review. exploration encompasses broad spectrum, from reducing through enhancing nutritional profiles fostering culinary innovation. Through detailed examination specific instances, challenges, prospects linked paradigm shift, we provide insights into transformative potential agriculture shaping more resilient system.

Language: Английский

Citations

3
Influence of Harvesting Stage on Phytochemical Composition, Antioxidant, and Antidiabetic Activity of Immature Ceratonia siliqua L. Pulp from Béni Mellal-Khénifra Region, Morocco: In Silico, In Vitro, and In Vivo Approaches DOI Creative Commons
Salah Laaraj, Hanane Choubbane, Amal Elrherabi

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(10), P. 10991 - 11020

Published: Sept. 29, 2024

Ceratonia siliqua L. is a medicinal plant that has long been used in traditional Moroccan medicine to treat many diseases. This study aimed assess the impact of stages immature phase carob pulp (M1, M2, M3, M4, and M5) on phytochemical composition, antioxidant activity, antidiabetic activity The identification phenolic profile by HPLC-UV/MS-MS effect silico, vitro, vivo studies were carried out for extracts with high contents compounds from wild communes Timoulit (TM), Bin Elouidane (AW), Ouaouizerth (TG) province Azilal Béni Mellal-Khénifra region. results revealed gradual increase total sugar content over pulp’s ripening period, reaching value 2134 ± 56.23 mg GE/100 g fresh weight (FW) TG. three locations showed peak values polyphenol (TPC), flavonoid (TFC), condensed tannin (TCT) at M2 stage. AW had highest concentrations TPC (3819 226.4 GAE/100 FM), TFC (1034 57.08 QE/100 TCT (1472 28.46 CE/100 FM). DPPH assay (7892 296.1 TE/100 FM) FRAP (278.2 7.85 both demonstrated TG zone highly potent zone. In contrast, site exhibited markedly elevated 725.4 103.6 FM ABTS assay. HPLC-UV-MS/MS analysis methanolic (MEICP) areas contained several different chemical compounds. most prevalent 3-O-p-coumaroyl-5-O-caffeoylquinic acid, quercetin 3-methyl ether, gallic galloylquinic acid. Immature extract (ICPE) strongest vitro inhibition pancreatic α-amylase (IC50 = 0.405 µg/mL) against intestinal α-glucosidase 0.063 µg/mL). vivo, AW, TG, TM significantly reduced postprandial glycemia rats, having greatest effect. These highlight potential ICPE. 3-O-p-Coumaroyl-5-O-caffeoylquinic acid better affinity compared acarbose interacted amino residues enzyme. Similarly, this molecule 3,4-Dicaffeoylquinic strong α-glucosidase, suggesting their as natural inhibitors enzymes involved carbohydrate metabolism. Most are not substrates P-glycoprotein absorption. Furthermore, majority these did act or CYP450 enzymes, reinforcing suitability development oral medications. underscore promising agent.

Language: Английский

Citations

3
Nutritional value, HPLC-DAD analysis and biological activities of Ceratonia siliqua L. pulp based on in vitro and in silico studies DOI Creative Commons
Salah Laaraj, Aziz Tikent, Chaimae El-rhouttais

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 28, 2024

Language: Английский

Citations

2
Analysis of genetic diversity of Moroccan natural carob (Ceratonia siliqua L.) populations using ISSR markers DOI

Hasna Zaggoumi,

Said Bouda, Hassane Abd‐dada

et al.

Vegetos, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 28, 2024

Language: Английский

Citations

1
Evaluating the Anti-Osteoporotic Potential of Mediterranean Medicinal Plants: A Review of Current Evidence DOI Creative Commons

Al-Hareth AbdulRaheem Salem Al-Ajalein,

Nurul ‘Izzah Ibrahim, Mh Busra Fauzi

et al.

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(10), P. 1341 - 1341

Published: Oct. 8, 2024

Bones are biological reservoirs for minerals and cells, offering protection to the other organs contributing structural form of body. Osteoporosis is a prevalent bone condition that significantly impacts people's quality life. Treatments utilizing natural products medicinal plants have gained important attention in management osteoporosis its associated implications, such as osteoporotic fractures. Even though thousands grow Mediterranean region, use an alternative therapy still limited.

Language: Английский

Citations

1
Evaluation of the Morphological and Physicochemical Diversity of Carob (Ceratonia siliqua, Fabaceae) Germplasm from Algeria DOI Open Access
Oussama Smaili, N. Chebouti-Meziou, Francesco Scollo

et al.

Forests, Journal Year: 2024, Volume and Issue: 15(8), P. 1423 - 1423

Published: Aug. 13, 2024

Despite the economic and ecological importance of carob tree, few studies are available on morphological characterization chemical composition in Algeria. This has resulted lack selection commercial cultivars high seed sugar content. The present study aims to assess diversity 11 wild 1 cultivated populations various regions Algeria, characterized by diverse geographical climatic conditions. final objective is neglected plants that exhibit superior fruit quality traits and/or well adapted different pedoclimatic current focuses analyzing pod investigating 12 5 physiochemical traits. analysis variance revealed significant differences between (p < 0.001) for all studied, indicating variability among these populations. Principal component (PCA) was employed relationship origin. High yields populations, whereas were rich sugar. Based pods, seeds compositions, a hierarchical clustering grouped into four groups. findings this represent advancement towards establishing effective cultivar breeding programs They demonstrate germplasm examined research exhibits an optimal level diversity, which instrumental identification specific both environmental significance.

Language: Английский

Citations

0
Carob seeds as a source of bioactive flavonoid derivatives: isolation, network pharmacology‐guided anti‐cancer activity, and HPLC standardization DOI
Asmaa M. Khalil, Nesrin M. Fayek, Omar M. Sabry

et al.

Chemistry & Biodiversity, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Abstract Carob, Ceratonia siliqua L. (CS), is a legume well‐known for its edible pod pulp. Its seeds are used almost exclusively as source of the food additive E410. Although variety metabolites have been identified by HPLC and LC–MS analysis in CS, reports concerned with their isolation scarce. In this study, two flavonoid derivatives were isolated from methanolic extract CS seeds, namely, quercetin‐3‐ O ‐rhamnoside 4′‐ p ‐hydroxybenzoylisorhamnetin‐3,7‐di‐ ‐rhamnoside. Network pharmacology was unusually guide estimation biological potential compounds. Finally, ethyl acetate fraction standardized content HPLC. The isolates displayed ability to interfere activity several target proteins associated renal colon cancers. Their cytotoxic effect on colorectal cancer cell lines investigated comparison Doxorubicin. selectivity compounds evaluated normal human fetal fibroblast lines. showed very potent against tested highest selectivity. can be bioactive that incorporated pharmaceutical industries.

Language: Английский

Citations

0