Eosinophil-Airway Epithelial Cells crosstalk reveals the eosinophils-mediated DUOX1 up-regulation in a murine allergic inflammation setting DOI Creative Commons
Carla Raggi, Francesca Spadaro, Fabrizio Mattei

et al.

Journal of Leukocyte Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 24, 2024

Abstract Blood and airway eosinophilia represent markers for the endotype-driven treatment of allergic asthma. Little is known on mechanisms that link eosinophils epithelial cells before after these are infiltrated by during response. Given innate immune mechanisms, mainly mediated epithelial-derived cytokines (interleukin [IL]-33, IL-25, TSLP [thymic stromal lymphopoietin]), induce eosinophil-maturing/attractive substances, we thought to evaluate crosstalk between in context IL-33–mediated inflammation. DUOX1 was previously described clinically relevant aspects inflammation a HDM -induced asthma mice model, patients with chronic sinusitis or Thus, evaluated involvement regulation cells. To recapitulate lung environment present at allergen challenge time acute asthma, set up an vitro model based murine bone marrow–derived differentiated IL-5 then activated IL-33 (EOs33) TC1 C57 We found induced eosinophil-attractive increased expression. Importantly, coculture EOs33 conditioned medium from enhanced expression DUOX1, which further combined stimulation (HDM plus EOs33). Our results suggest recruited once could be involved loop DUOX1-mediated secretion.

Language: Английский

Neochlorogenic acid ameliorates allergic airway inflammation by suppressing type 2 immunity and upregulating HO-1 expression DOI
Shu‐Chen Cheng, Chian‐Jiun Liou, Shu‐Ju Wu

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 146, P. 113867 - 113867

Published: Dec. 16, 2024

Language: Английский

Citations

4
Eosinophils and pleural macrophages counter regulate IL-33-elicited airway inflammation via the 12/15-lipoxygenase pathway DOI Creative Commons
E. Ito,

Reika Hayashizaki,

Tetsuya Hosaka

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 17, 2025

Fatty acid metabolism plays a crucial role in regulating airway inflammation through the synthesis of lipid mediators. We have previously demonstrated that 12/15-lipoxygenase (12/15-LOX or Alox15)-derived mediator attenuates IL-33-induced eosinophilic mice. However, cellular sources these mediators remain unclear. To identify sources, we used several cell type-specific conditional 12/15-LOX-deficient found eosinophils and pleural macrophages were major 12/15-LOX-expressing types responsible for attenuating inflammation. Eosinophils population cells inflamed lung tissue. In addition, thoracic cavity to translocate into tissue response This study suggests cooperatively regulate via 12/15-LOX expression. Targeting may offer new therapeutic strategies severe asthma.

Language: Английский

Citations

0
Pulsed 980 nm Near-Infrared Photobiomodulation Attenuates OVA-Induced Allergic Asthma by Modulating Th2 Cytokine Responses and Inflammatory Cell Infiltration DOI

Zhengyi Wu,

Jia Zhang, Qingxiang Gao

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

Abstract Photobiomodulation (PBM) is a non-invasive therapeutic strategy that uses specific light wavelengths to stimulate cellular processes promote tissue repair, reduce inflammation, and alleviate pain. In this study, we evaluated the efficacy of high-intensity 980 nm near-infrared (NIR) pulsed laser in murine model ovalbumin (OVA)-induced allergic asthma. OVA-sensitized mice, typical features were noted, including airway wall thickening, significant peribronchial perivascular inflammatory cell infiltration, compressed alveolar spaces. NIR PBM treatment markedly attenuated these histological changes. At molecular level, significantly downregulated type 2 cytokine gene expression, IL-4, IL-5 IL-13, lung tissue. Flow cytometry analyses further revealed reduced infiltration pulmonary eosinophils monocytes. Transcriptomic profiling coupled with set enrichment analysis (GSEA) indicated suppressed NF-κB-mediated signaling while modulating Th2-related responses. Collectively, our findings demonstrate effectively mitigates key asthma model, supporting its potential as non-pharmacological, adjunct therapy for management.

Language: Английский

Citations

0
Eosinophil-Airway Epithelial Cells crosstalk reveals the eosinophils-mediated DUOX1 up-regulation in a murine allergic inflammation setting DOI Creative Commons
Carla Raggi, Francesca Spadaro, Fabrizio Mattei

et al.

Journal of Leukocyte Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 24, 2024

Abstract Blood and airway eosinophilia represent markers for the endotype-driven treatment of allergic asthma. Little is known on mechanisms that link eosinophils epithelial cells before after these are infiltrated by during response. Given innate immune mechanisms, mainly mediated epithelial-derived cytokines (interleukin [IL]-33, IL-25, TSLP [thymic stromal lymphopoietin]), induce eosinophil-maturing/attractive substances, we thought to evaluate crosstalk between in context IL-33–mediated inflammation. DUOX1 was previously described clinically relevant aspects inflammation a HDM -induced asthma mice model, patients with chronic sinusitis or Thus, evaluated involvement regulation cells. To recapitulate lung environment present at allergen challenge time acute asthma, set up an vitro model based murine bone marrow–derived differentiated IL-5 then activated IL-33 (EOs33) TC1 C57 We found induced eosinophil-attractive increased expression. Importantly, coculture EOs33 conditioned medium from enhanced expression DUOX1, which further combined stimulation (HDM plus EOs33). Our results suggest recruited once could be involved loop DUOX1-mediated secretion.

Language: Английский

Citations

0