Comparative Efficacy of 3% Diquafosol Sodium Eye Drops in Dry Eye Patients Across Age Groups: a Propensity Score-Matched Longitudinal Study DOI Creative Commons
Yi Dong, Zecheng Li, Luxia Chen

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 16, 2024

Abstract This study aimed to evaluate the efficacy of 3% diquafosol sodium eye drops in treating dry disease (DED) across different age groups using a propensity score-matched longitudinal design. Participants were divided into younger (< 40 years) and older (≥ groups. Propensity score matching (PSM) was performed at 1:1 ratio, ensuring comparable baseline characteristics. All participants received drops, administered six times daily for 3 months. Clinical metrics, including Dry Eye Questionnaire Score (DEQS), Fluorescein Break-Up Time (FBUT), Tear Meniscus Height (TMH), Schirmer I, Lipid Layer Thickness, Logarithm Minimum Angle Resolution, Intraocular Pressure, Meibomian Gland Loss Area, assessed (V1), 1 month (V2), months (V3). Of 100 DED participants, 97 completed study. Baseline characteristics did not significantly differ between post-PSM. Metrics V2 V3 significantly. Longitudinal mixed-effects models demonstrated significant improvements DEQS, FBUT, TMH over time both Diquafosol (3%) effectively improved symptoms groups, suggesting that does affect diquafosol’s efficacy, thereby supporting its use broad demographic patients with DED.

Language: Английский

Identification of Meibomian gland stem cell populations and mechanisms of aging DOI Creative Commons
Xuming Zhu, Mingang Xu, Céline Portal

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 15, 2025

Language: Английский

Citations

1
Chronic sleep deprivation impairs retinal circadian transcriptome and visual function DOI

Shenzhen Huang,

Wenxiao Zhang,

Shuting Xuan

et al.

Experimental Eye Research, Journal Year: 2024, Volume and Issue: 243, P. 109907 - 109907

Published: April 21, 2024

Language: Английский

Citations

7
Exploring Immune Cell Diversity in the Lacrimal Glands of Healthy Mice: A Single-Cell RNA-Sequencing Atlas DOI Open Access

Qiwei Fan,

Ruyu Yan,

Yan Li

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 1208 - 1208

Published: Jan. 19, 2024

The lacrimal gland is responsible for maintaining the health of ocular surface through production tears. However, our understanding immune system within currently limited. Therefore, in this study, we utilized single-cell RNA sequencing and bioinformatic analysis to identify analyze cells molecules present glands normal mice. A total 34,891 were obtained from mice classified into 18 distinct cell clusters using Seurat clustering. Within these populations, 26 different subpopulations identified, including T cells, innate lymphocytes, macrophages, mast dendritic B cells. Network revealed complex cell-cell interactions between with particularly significant observed among plasma Interestingly, found be main source ligands Thy1 signaling pathway, while M2 macrophages identified as primary target pathway. Moreover, some validated immunohistological techniques. Collectively, findings highlight abundance provide valuable insights complexity its relevance associated diseases.

Language: Английский

Citations

6
PINK1/Parkin-Mediated Mitophagy Ameliorates Mitochondrial Dysfunction in Lacrimal Gland Acinar Cells During Aging DOI Creative Commons
Han Zhao, Yue Zhang, Yujie Ren

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2024, Volume and Issue: 65(13), P. 12 - 12

Published: Nov. 6, 2024

Purpose: Aging alters the function of lacrimal gland and disrupts balance microenvironment on ocular surface, eventually leading to aqueous-tear-deficient dry eye. Mitophagy has been reported play an important role in aging, but underlying mechanism remains unclear. Methods: The young (6 weeks) middle-aged (12 months) male C57BL/6J mice were used this study, mitophagy agonist rapamycin inhibitor Mdivi-1 vivo experiments. Hematoxylin eosin, Masson, Oil Red O, reactive oxygen species (ROS) staining detect histological changes lipids gland. Changes expression proteins identified by Western blotting lysates. Transmission electron microscopy immunofluorescence assess mitophagy. single-cell RNA sequencing (scRNA-seq) bioinformatics analyses transcription signature during aging. Results: In we discovered that aging increased oxidative stress, which apoptosis, generated ROS acinar epithelial cells. Furthermore, activation PINK1/Parkin-mediated reduced concentrations prevented aging-induced apoptosis cells, thereby causing alterations, microstructural degradation, increasing tear secretion associated with accumulation. By contrast, aggregates mitochondrial thereafter leads impairment inhibiting fission giving rise Conclusions: Overall, our findings suggested could impair age-related alterations may be treated therapeutic approaches enhance while maintaining function.

Language: Английский

Citations

4
Chronic Jet Lag Disrupts Circadian Rhythms and Induces Hyperproliferation in Murine Lacrimal Glands via ROS Accumulation DOI Creative Commons

Shenzhen Huang,

Wenxiao Zhang,

Mengru Ba

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(1), P. 12 - 12

Published: Jan. 7, 2025

Purpose: Chronic jet lag (CJL) is known to disrupt circadian rhythms, which regulate various physiological processes, including ocular surface homeostasis. However, the specific effects of CJL on lacrimal gland function and underlying cellular mechanisms remain poorly understood. Methods: A model was established using C57BL/6J mice. Extraorbital glands (ELGs) were collected at 3-hour intervals for RNA extraction high-throughput sequencing. Circadian transcriptomic profiles analyzed, functional annotations performed. Hydrogen peroxide levels total antioxidant capacity in tear fluid measured chemometric assays. Immunofluorescence used assess cell proliferation, apoptosis, immune infiltration ELGs, reactive oxygen species (ROS) accumulation. The potential therapeutic alpha-lipoic acid (ALA) CJL-induced oxidative stress pathological changes ELGs also investigated. Results: significantly disrupted locomotor activity, altered body temperature modified diurnal oscillations ELGs. Transcriptomic analysis revealed extensive rhythmic gene expression, phase shifts, pathway clustering response CJL. disruption core clock transcription associated with ELG hyperproliferation increased ROS tert-Butyl hydroperoxide promoted ALA effectively reduced mitigated hyperproliferation. Conclusions: These findings uncover novel molecular pathways affected by highlight therapies, such as ALA, preserving health under conditions rhythm disruption.

Language: Английский

Citations

0
Melatonin Alleviates Age-Related Lacrimal Gland Dysfunction Via SIRT-1/NLRP3 Pathway DOI Creative Commons
Chao Wang, Yuzhi Li, Huan Guo

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(2), P. 51 - 51

Published: Feb. 20, 2025

As a consequence of the natural aging process, lacrimal glands may become dysfunctional. The present study aimed to investigate potential role melatonin (MLT) in alleviation age-related gland dysfunction and elucidate underlying mechanism. In this study, 2-month-old, 18-month-old, MLT intraperitoneally injected 18-month-old mice were obtained for immunofluorescence, immunohistochemistry experiments, Western blotting detect inflammatory factors AQP5 expression, electron microscopy mitochondrial structure dense granules. Lacrimal from taken cell culture, PCR performed signaling pathways which acts. addition, human explant cultures validate SIRT-1/NLRP3 pathways. we discovered that increased response, decreased secretory function, led dysregulation gland. Compared with 2-month-old mice, SIRT-1/3/6 gene transcript levels significantly mice. reduced (IL-1β, IL-6, TNF-α) expression via pathway aged mouse. Furthermore, restored granules mouse explants gland, relieved fibrosis. demonstrated alleviates humans pathway. alleviated response decline function

Language: Английский

Citations

0
Advances in lacrimal gland organoid development: Techniques and therapeutic applications DOI
Ahmed Abdal Dayem, Soo Bin Jang,

Nahee Lim

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2025, Volume and Issue: 183, P. 117870 - 117870

Published: Jan. 26, 2025

Language: Английский

Citations

0
Sleep Deprivation Potentiates Silica Nanoparticle-Induced Circadian Disruption and Lacrimal Gland Dysfunction in Mice DOI
Wenxiao Zhang, Di Qi, Xiaoting Pei

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: May 9, 2025

Abstract Sleep deprivation and engineered nanomaterials, such as silica nanoparticles, have emerged potential risk factors for ocular surface disorders, including dry eye disease. However, the mechanisms by which sleep exacerbates nanoparticle-induced lacrimal gland dysfunction remain unclear. In this study, combined effects of nanoparticle exposure on circadian rhythms, oxidative stress, inflammation, structural integrity extraorbital were investigated in mice. Male C57BL/6J mice divided into control, nanoparticle-treated, groups. Locomotor activity, core body temperature, tear production, glandular morphology assessed, while RNA sequencing was performed to examine gene expression inflammatory pathways. Oxidative stress evaluated via reactive oxygen species accumulation γ-H2AX expression, inflammation assessed immune cell infiltration NLRP3 inflammasome activation. Silica dampened behavioral temperature these further exacerbated deprivation, also abolished diurnal secretion. Histological transcriptomic analyses revealed reduced size, phase shifts clock elevated DNA damage, increased activation NF-κB, IL-17, JAK-STAT The markedly activated group, linking chronic tissue dysfunction. These findings demonstrate that aggravates injury disrupting homeostasis promoting highlighting importance addressing environmental stressors prevention treatment deprivation-associated

Language: Английский

Citations

0
Long-term high fructose intake reprograms the circadian transcriptome and disrupts homeostasis in mouse extra-orbital lacrimal glands DOI
Di Qi,

Duliurui Huang,

Mengru Ba

et al.

Experimental Eye Research, Journal Year: 2024, Volume and Issue: 246, P. 110008 - 110008

Published: July 16, 2024

Language: Английский

Citations

2
Identification of Meibomian gland stem cell populations and mechanisms of aging DOI Creative Commons
Xuming Zhu, Mingang Xu, Céline Portal

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 10, 2024

Summary Meibomian glands secrete lipid-rich meibum, which prevents tear evaporation. Aging-related gland shrinkage may result in part from stem cell exhaustion and is associated with evaporative dry eye disease, a common condition lacking effective treatment. The identities niche of cells the signals controlling their activity are poorly defined. Using snRNA-seq, vivo lineage tracing, ex live imaging, genetic studies mice, we identified markers for populations that maintain distinct regions uncovered Hh signaling as key regulator proliferation. Consistent this, human carcinoma exhibited increased signaling. Aged displayed decreased EGF signaling, deficient innervation, loss collagen I fibroblasts, indicating alterations both glandular epithelial surrounding microenvironment contribute to age-related degeneration. These findings suggest new approaches treat aging-associated loss.

Language: Английский

Citations

0