The Impact of Single-Cell Genomics on Adipose Tissue Research DOI Open Access
Alana Deutsch, Daorong Feng, Jeffrey E. Pessin

et al.

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(13), P. 4773 - 4773

Published: July 5, 2020

Adipose tissue is an important regulator of whole-body metabolism and energy homeostasis. The unprecedented growth obesity metabolic disease worldwide has required paralleled advancements in research on this dynamic endocrine organ system. Single-cell RNA sequencing (scRNA-seq), a highly meticulous methodology used to dissect heterogeneity through the transcriptional characterization individual cells, responsible for facilitating critical area. unique investigative capabilities achieved by combination nanotechnology, molecular biology, informatics are expanding our understanding adipose tissue’s composition compartmentalized functional specialization, which underlie physiologic pathogenic states, including adaptive thermogenesis, aging, obesity. In review, we will summarize use scRNA-seq single-nuclei RNA-seq (snRNA-seq) adipocyte biology their applications diabetes hopes increasing awareness technology acting as catalyst its expanded further investigation.

Language: Английский

UCP1-independent thermogenesis DOI

Anna Roesler,

Lawrence Kazak

Biochemical Journal, Journal Year: 2020, Volume and Issue: 477(3), P. 709 - 725

Published: Feb. 14, 2020

Obesity results from energy imbalance, when intake exceeds expenditure. Brown adipose tissue (BAT) drives non-shivering thermogenesis which represents a powerful mechanism of enhancing the expenditure side balance equation. The best understood thermogenic system in BAT that evolved to protect body hypothermia is based on uncoupling protonmotive force oxidative phosphorylation through actions protein 1 (UCP1), key regulator cold-mediated thermogenesis. Similarly, triggered response caloric excess, and animals with reduced fat function can succumb diet-induced obesity. Thus, it was surprising inactivation Ucp1 did not potentiate In recent years, has become clear multiple mechanisms exist, ATP sinks centered creatine, lipid, or calcium cycling, along Fatty acid-mediated UCP1-independent leak pathways driven by ADP/ATP carrier (AAC). With difference between cold- being dynamic changes purine nucleotide (primarily ATP) levels, ATP-dependent may play role Additionally, ubiquitous expression AAC facilitate increased many cell types, face over feeding. Interest begun showcase therapeutic potential lies refining our understanding diversity biochemical controlling respiration.

Language: Английский

Citations

118
An update on brown adipose tissue biology: a discussion of recent findings DOI
Rafael Calais Gaspar, José Rodrigo Pauli, Gerald I. Shulman

et al.

AJP Endocrinology and Metabolism, Journal Year: 2021, Volume and Issue: 320(3), P. E488 - E495

Published: Jan. 18, 2021

Brown adipose tissue (BAT) has been encouraged as a potential treatment for obesity and comorbidities due to its thermogenic activity capacity contribution energy expenditure. Some interventions such cold β-adrenergic drugs are able activate BAT thermogenesis well promote differentiation of white adipocytes into brown-like cells (browning), enhancing the these cells. In this mini-review, we discuss new mechanisms related regard, will also recent studies that have revealed existence important secretory molecules from "batokines" act in autocrine, paracrine, endocrine mechanisms, which turn may explain some beneficial roles on whole body glucose fat metabolism. Finally, insights with an additional focus distinct features metabolism between rodents humans.

Language: Английский

Citations

79
Adipocyte iron levels impinge on a fat-gut crosstalk to regulate intestinal lipid absorption and mediate protection from obesity DOI Creative Commons
Zhuzhen Zhang, Jan‐Bernd Funcke, Zhenzhen Zi

et al.

Cell Metabolism, Journal Year: 2021, Volume and Issue: 33(8), P. 1624 - 1639.e9

Published: June 25, 2021

Language: Английский

Citations

78
Single cell approaches to address adipose tissue stromal cell heterogeneity DOI

Elizabeth A. Rondini,

James G. Granneman

Biochemical Journal, Journal Year: 2020, Volume and Issue: 477(3), P. 583 - 600

Published: Feb. 6, 2020

A central function of adipose tissue is in the management systemic energy homeostasis that achieved through co-ordinated regulation storage and mobilization, adipokine release, immune functions. With dramatic increase prevalence obesity obesity-related metabolic disease over past 30 years, there has been extensive interest targeting for therapeutic benefit. However, order this goal to be it essential establish a comprehensive atlas cellular composition define mechanisms intercellular communication mediate pathologic responses. While traditional methods, such as fluorescence-activated cell sorting (FACS) genetic lineage tracing, have greatly advanced field, these approaches are inherently limited by choice markers ability comprehensively identify characterize dynamic interactions among stromal cells within microenvironment. Single RNA sequencing (scRNAseq) emerged powerful tool deconvolving heterogeneity holds promise understanding development plasticity under normal pathological conditions. scRNAseq recently used stem (ASC) populations provided new insights into subpopulations macrophages arise during anabolic catabolic remodeling white tissue. The current review summarizes recent findings use technology explore plasticity.

Language: Английский

Citations

71
Aging adipose: Depot location dictates age-associated expansion and dysfunction DOI
Helaina Von Bank,

Charlie Kirsh,

Judith Simcox

et al.

Ageing Research Reviews, Journal Year: 2021, Volume and Issue: 67, P. 101259 - 101259

Published: Jan. 27, 2021

Language: Английский

Citations

70
Adipose Tissue Remodeling in Pathophysiology DOI Creative Commons
Christopher Auger, Shingo Kajimura

Annual Review of Pathology Mechanisms of Disease, Journal Year: 2022, Volume and Issue: 18(1), P. 71 - 93

Published: Sept. 7, 2022

Rather than serving as a mere onlooker, adipose tissue is complex endocrine organ and active participant in disease initiation progression. Disruptions of biological processes operating within can disturb healthy systemic physiology, the sequelae which include metabolic disorders such obesity type 2 diabetes. A burgeoning interest field research has allowed for elucidation regulatory networks underlying both function dysfunction. Despite this progress, few diseases are treated by targeting maladaptation adipose, an oft-overlooked organ. In review, we elaborate on distinct subtypes adipocytes, their developmental origins secretory roles, dynamic interplay at work itself. Central to discussion relationship between states, including obesity, cachexia, infectious diseases, aim leverage our wealth knowledge development novel targeted therapeutics.

Language: Английский

Citations

66
The sympathetic nervous system in the 21st century: Neuroimmune interactions in metabolic homeostasis and obesity DOI Creative Commons
Noelia Martínez‐Sánchez,

Owen Sweeney,

Davi Sidarta-Oliveira

et al.

Neuron, Journal Year: 2022, Volume and Issue: 110(21), P. 3597 - 3626

Published: Nov. 1, 2022

Language: Английский

Citations

57
Characterization of transcript enrichment and detection bias in single-nucleus RNA-seq for mapping of distinct human adipocyte lineages DOI Creative Commons
Anushka Gupta, Farnaz Shamsi, Nicolas Altemose

et al.

Genome Research, Journal Year: 2022, Volume and Issue: 32(2), P. 242 - 257

Published: Jan. 18, 2022

Single-cell RNA sequencing (scRNA-seq) enables molecular characterization of complex biological tissues at high resolution. The requirement single-cell extraction, however, makes it challenging for profiling such as adipose tissue, which collection intact single adipocytes is complicated by their fragile nature. For tissues, single-nucleus extraction often much more efficient and therefore (snRNA-seq) presents an alternative to scRNA-seq. However, nuclear transcripts represent only a fraction the transcriptome in cell, with snRNA-seq marked inherent transcript enrichment detection biases. Therefore, may be inadequate mapping important transcriptional signatures tissue. In this study, we compare transcriptomic landscape nuclei isolated from preadipocytes mature across human white brown adipocyte lineages, whole-cell transcriptome. We show that capable identifying broad cell types present scRNA-seq all states adipogenesis. also explore how why biased limited, well can advantageous. robustly characterize nuclear-localized adipogenic regulatory lncRNAs snRNA-seq, while providing detailed understanding preferential long genes upon using technique. To remove technical biases, propose normalization strategy accurate comparison cellular data. Finally, successful integration data sets existing bioinformatic tools. Overall, our results illustrate applicability diversity

Language: Английский

Citations

46
Adipocyte IRE1α promotes PGC1α mRNA decay and restrains adaptive thermogenesis DOI
Yong Chen, Zhuyin Wu, Shijia Huang

et al.

Nature Metabolism, Journal Year: 2022, Volume and Issue: 4(9), P. 1166 - 1184

Published: Sept. 19, 2022

Language: Английский

Citations

40
Mitochondrial Energy Metabolism in the Regulation of Thermogenic Brown Fats and Human Metabolic Diseases DOI Open Access
Yukimasa Takeda, Yoshinori Harada,

Toshikazu Yoshikawa

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(2), P. 1352 - 1352

Published: Jan. 10, 2023

Brown fats specialize in thermogenesis by increasing the utilization of circulating blood glucose and fatty acids. Emerging evidence suggests that brown adipose tissue (BAT) prevents incidence obesity-associated metabolic diseases several types cancers humans. Mitochondrial energy metabolism brown/beige adipocytes regulates both uncoupling protein 1 (UCP1)-dependent -independent for cold adaptation excess nutrients energy. Many studies on quantification human BAT indicate mass activity are inversely correlated with body index (BMI) visceral adiposity. Repression is caused positive negative factors control adipocyte browning, de novo adipogenesis, mitochondrial metabolism, UCP1 expression activity, noradrenergic response. Systemic local whose levels vary between lean obese conditions include growth factors, inflammatory cytokines, neurotransmitters, metal ions such as selenium iron. Modulation repression a promising strategy to counteract obesity related through activation thermogenic capacity. In this review, we highlight recent advances regulation fats, diseases.

Language: Английский

Citations

32