Multi-omics microsampling for the profiling of lifestyle-associated changes in health

Nature Biomedical Engineering, Journal Year: 2023, Volume and Issue: 8(1), P. 11 - 29

Published: Jan. 19, 2023

Abstract Current healthcare practices are reactive and use limited physiological clinical information, often collected months or years apart. Moreover, the discovery profiling of blood biomarkers in research settings constrained by geographical barriers, cost inconvenience in-clinic venepuncture, low sampling frequency depth molecular measurements. Here we describe a strategy for frequent capture analysis thousands metabolites, lipids, cytokines proteins 10 μl alongside information from wearable sensors. We show advantages such dense multi-omics microsampling two applications: assessment reactions to complex mixture dietary interventions, discover individualized inflammatory metabolic responses; deep profiling, reveal large-scale fluctuations as well relationships associated with intra-day variations (in heart rate, example) levels (specifically, glucose cortisol) physical activity. Combining wearables scalable omics may facilitate dynamic health biomarker discovery.

Language: Английский

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Wearables in Chronomedicine and Interpretation of Circadian Health

Diagnostics, Journal Year: 2025, Volume and Issue: 15(3), P. 327 - 327

Published: Jan. 30, 2025

Wearable devices have gained increasing attention for use in multifunctional applications related to health monitoring, particularly research of the circadian rhythms cognitive functions and metabolic processes. In this comprehensive review, we encompass how wearables can be used study disease. We highlight importance these as markers well-being potential predictors outcomes. focus on wearable technologies sleep research, medicine, chronomedicine beyond domain emphasize actigraphy a validated tool monitoring sleep, activity, light exposure. discuss various mathematical methods currently analyze actigraphic data, such parametric non-parametric approaches, linear, non-linear, neural network-based applied quantify non-circadian variability. also introduce novel actigraphy-derived markers, which personalized proxies status, assisting discriminating between disease, offering insights into neurobehavioral status. lifestyle factors physical activity exposure modulate brain health. establishing reference standards measures further refine data interpretation improve clinical The review calls existing tools methods, deepen our understanding health, develop healthcare strategies.

Language: Английский

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The circadian system, sleep, and the health/disease balance: a conceptual review

Journal of Sleep Research, Journal Year: 2022, Volume and Issue: 31(4)

Published: June 7, 2022

Summary The field of “circadian medicine” is a recent addition to chronobiology and sleep research efforts. It represents logical step arising from the increasing insights into circadian system its interactions with life in urbanised societies; applying these health/disease balance at home medical practice (outpatient) clinic (inpatient). Despite fast expansion proliferating efforts, medicine lacks formal framework categorise many observations describing among system, sleep, balance. A good allows us then assign them one or more components hypothesised interactions. Such assignments can lead experiments that document causal (rather than correlational) relationships move discovering mechanisms. This review details such proposed for will hopefully trigger discussion our colleagues, so be improved expanded. As basis medicine, we define health” how it links general health. We put context literature examples six domains balance: fertility, cancer, immune mental health, cardiovascular, metabolism.

Language: Английский

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Circadian clocks of the kidney: function, mechanism, and regulation

Physiological Reviews, Journal Year: 2022, Volume and Issue: 102(4), P. 1669 - 1701

Published: May 16, 2022

An intrinsic cellular circadian clock is located in nearly every cell of the body. The peripheral clocks within cells kidney contribute to regulation a variety renal processes. In this review, we summarize what currently known regarding function, mechanism, and clocks. Additionally, effect extrarenal physiological processes, such as endocrine neuronal signals, on function also reviewed. Circadian rhythms are an integral part physiology, underscoring importance considering time day key biological variable. field physiology tremendous relevance, but with limited mechanistic information area need extensive investigation.

Language: Английский

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Liver and muscle circadian clocks cooperate to support glucose tolerance in mice

Cell Reports, Journal Year: 2023, Volume and Issue: 42(6), P. 112588 - 112588

Published: June 1, 2023

Physiology is regulated by interconnected cell and tissue circadian clocks. Disruption of the rhythms generated concerted activity these clocks associated with metabolic disease. Here we tested interactions between in two critical components organismal metabolism, liver skeletal muscle, rescuing clock function either each organ separately or both organs simultaneously otherwise clock-less mice. Experiments showed that individual are partially sufficient for glucose yet connections coupled to daily feeding support systemic tolerance. This synergy relies part on local transcriptional control machinery, feeding-responsive signals such as insulin, cycles connect muscle liver. We posit spatiotemporal mechanisms play an essential role maintenance homeostasis disrupting this diurnal coordination can contribute

Language: Английский

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Association between accelerometer-measured amplitude of rest–activity rhythm and future health risk: a prospective cohort study of the UK Biobank

The Lancet Healthy Longevity, Journal Year: 2023, Volume and Issue: 4(5), P. e200 - e210

Published: May 1, 2023

BackgroundThe health effects of rest–activity rhythm are major interest to public health, but its associations with outcomes remain elusive. We aimed examine the between accelerometer-measured amplitude and risks among general UK population.MethodsWe did a prospective cohort analysis Biobank participants aged 43–79 years valid wrist-worn accelerometer data. Low was defined as first quintile relative amplitude; all other quintiles were classified high amplitude. Outcomes using International Classification Diseases 10th Revision codes consisted incident cancer cardiovascular, infectious, respiratory, digestive diseases, all-cause disease-specific (cardiovascular, cancer, respiratory) mortality. Participants current diagnosis any outcome excluded. assessed decreased Cox proportional hazards models.FindingsBetween June 1, 2013, Dec 23, 2015, 103 682 available raw data enrolled. 92 614 (52 219 [56·4%] women 40 395 [42·6%] men) median age 64 (IQR 56–69) recruited. Median follow-up 6·4 5·8–6·9). Decreased significantly associated increased incidence cardiovascular diseases (adjusted hazard ratio 1·11 [95% CI 1·05–1·16]), (1·08 [1·01–1·16]), infectious (1·31 [1·22–1·41]), respiratory (1·26 [1·19–1·34]), [1·03–1·14]), well mortality (1·54 [1·40–1·70]) (1·73 [1·34–2·22] for 1·32 [1·13–1·55] 1·62 [1·25–2·09] diseases). Most these not modified by older than 65 or sex. Among 16 parameters, low had strongest second- nine outcomes.InterpretationOur results suggest that might contribute provide further evidence promote risk-modifying strategies improve longevity.FundingNational Natural Science Foundation China Postdoctoral Foundation.

Language: Английский

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ENLIGHT: A consensus checklist for reporting laboratory-based studies on the non-visual effects of light in humans

EBioMedicine, Journal Year: 2023, Volume and Issue: 98, P. 104889 - 104889

Published: Dec. 1, 2023

BackgroundThere is no consensus on reporting light characteristics in studies investigating non-visual responses to light. This project aimed develop a checklist for laboratory-based investigations the impact of physiology.MethodsA four-step modified Delphi process (three questionnaire-based feedback rounds and one face-to-face group discussion) involving international experts was conducted reach items be included checklist. Following process, resulting tested pilot phase with independent experts.FindingsAn initial list 61 related light-based interventions condensed final containing 25 items, based upon among (final n = 60). Nine were deemed necessary report regardless research question or context. A description each item provided accompanying Explanation Elaboration (E&E) document. The testing led minor textual clarifications E&E document.InterpretationThe ENLIGHT Checklist first consensus-based documenting ocular human studies. implementation will enhance by ensuring comprehensive documentation, enhancing reproducibility, enabling data aggregation across studies.FundingNetwork European Institutes Advanced Study (NETIAS) Constructive Thinking (CAT) programme; Sir Henry Wellcome Postdoctoral Fellowship (Wellcome Trust, 204686/Z/16/Z); Netherlands Organisation Health Research Development VENI fellowship (2020–09150161910128); U.S. Department Defense Grant (W81XWH-16-1-0223); National University Singapore (NUHSRO/2022/038/Startup/08); Foundation (NRF2022-THE004-0002).

Language: Английский

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The REV-ERB Nuclear Receptors: Timekeepers for the Core Clock Period and Metabolism

Endocrinology, Journal Year: 2023, Volume and Issue: 164(6)

Published: April 17, 2023

REV-ERB nuclear receptors are potent transcriptional repressors that play an important role in the core mammalian molecular clock and metabolism. Deletion of both REV-ERBα its largely redundant isoform REV-ERBβ a murine tissue-specific manner have shed light on their specific functions mechanisms circadian This review highlights recent findings establish REV-ERBs as crucial timekeepers variety tissues, regulating overlapping distinct processes maintain normal physiology protect from metabolic dysfunction.

Language: Английский

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Natural Compounds for Preventing Age-Related Diseases and Cancers

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7530 - 7530

Published: July 9, 2024

Aging is a multifaceted process influenced by hereditary factors, lifestyle, and environmental elements. As time progresses, the human body experiences degenerative changes in major functions. The external internal signs of aging manifest various ways, including skin dryness, wrinkles, musculoskeletal disorders, cardiovascular diseases, diabetes, neurodegenerative cancer. Additionally, cancer, like aging, complex disease that arises from accumulation genetic epigenetic alterations. Circadian clock dysregulation has recently been identified as an important risk factor for cancer development. Natural compounds herbal medicines have gained significant attention their potential preventing age-related diseases inhibiting progression. These demonstrate antioxidant, anti-inflammatory, anti-proliferative, pro-apoptotic, anti-metastatic, anti-angiogenic effects well circadian regulation. This review explores cancers, specific natural targeting key features these conditions.

Language: Английский

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A Transcriptomic Dataset of Liver Tissues from Global and Liver-Specific Bmal1 Knockout Mice

Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)

Published: Feb. 3, 2025

The circadian clock regulates various physiological processes in mammals. core gene Bmal1 is crucial for maintaining the oscillations of system by controlling rhythmic expression numerous clock-controlled genes. To explore transcriptional changes associated with deletion liver tissues, we collected tissues from global and liver-specific knockout mice, along their respective control groups, at two time points (CT2 CT14) used them transcriptome sequencing analysis. Genotyping, locomotor activity analysis, comprehensive quality analyses, including base scores, GC content, mapping rates, confirmed high data. Differential analysis RT-qPCR validation reliability validity dataset. These data offer a valuable resource researchers investigating role BMAL1 physiology, pathology, broader field biology.

Language: Английский

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