LINC01133 promotes pancreatic ductal adenocarcinoma epithelial–mesenchymal transition mediated by SPP1 through binding to Arp3 DOI Creative Commons
Yefan Yang,

Yuxi Gong,

Ying Ding

et al.

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(7)

Published: July 10, 2024

Pancreatic ductal adenocarcinoma (PDAC) is a lethal disease with limited treatment methods. Long non-coding RNAs (lncRNAs) have been found involved in tumorigenic and progression. The present study revealed that LINC01133, fewly reported lncRNA, was one of 16 hub genes could predict PDAC patients' prognosis. LINC01133 over-expressed tumors compared to adjacent pancreas promote proliferation metastasis vitro vivo, as well inhibit apoptosis. expression positively correlated secreted phosphoprotein 1 (SPP1) expression, leading an enhanced epithelial-mesenchymal transition (EMT) process. bound actin-related protein 3 (Arp3), the complex reduced SPP1 mRNA degradation which increased level, ultimately proliferation. This research novel mechanism development provided potential prognosis indicator may benefit patients.

Language: Английский

Neutrophil profiling illuminates anti-tumor antigen-presenting potency DOI Creative Commons
Yingcheng Wu, Jiaqiang Ma, Xupeng Yang

et al.

Cell, Journal Year: 2024, Volume and Issue: 187(6), P. 1422 - 1439.e24

Published: March 1, 2024

Language: Английский

Citations

101
Single-cell and spatial dissection of precancerous lesions underlying the initiation process of oral squamous cell carcinoma DOI Creative Commons
Lulu Sun, Xindan Kang, Chong Wang

et al.

Cell Discovery, Journal Year: 2023, Volume and Issue: 9(1)

Published: March 13, 2023

Precancerous lesions of the oral mucosa, especially those accompanied by moderate to severe dysplasia, contribute initiation squamous cell carcinoma (OSCC). However, cellular compositions and spatial organization precancerous stage how these factors promote human OSCC remain unclear. Here, we built a single-cell transcriptome atlas map after obtaining data from pairwise mucosal biopsies 9 individuals consisting very early-stage OSCC, adjacent with as well matched normal region. An altered epithelial gene-expression profile was identified which favored initiation. This observation coupled distinct fibroblast, monocytic, regulatory T-cell subclusters involved in reshaping microenvironment. In particular, unique immune-inhibitory monocyte subtype spatial-switching regulation VEGF signaling were observed surrounding lesions, concertedly strengthening activities promoting cancer Collectively, our work elucidated landscapes roles underlying initiation, is essential for understanding entire process helps inform therapeutic strategies intervention.

Language: Английский

Citations

38
Cancer‐Associated Fibroblast‐Induced Remodeling of Tumor Microenvironment in Recurrent Bladder Cancer DOI Creative Commons
Ting Liang,

Tao Tao,

Kai Wu

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(31)

Published: Sept. 24, 2023

Bladder carcinoma (BC) recurrence is a major clinical challenge, and targeting the tumor microenvironment (TME) promising therapy. However, relationship between individual TME components, particularly cancer-associated fibroblasts (CAFs), unclear. Here, heterogeneity in primary recurrent BC investigated using single-cell RNA sequence profiling of 62 460 cells. Two cancer stem cell (CSC) subtypes are identified BC. An inflammatory CAF subtype, ICAM1+ iCAFs, specifically associated with also identified. iCAFs found to secrete FGF2, which acts on CD44 receptor rCSC-M, thereby maintaining stemness epithelial-mesenchymal transition. Additionally, THBS1+ monocytes, group myeloid-derived suppressor cells (MDSCs), enriched interacted CAFs. CCL2, binds CCR2 MDSCs. Moreover, elevated STAT3, NFKB2, VEGFA, CTGF levels reshape tumors. CCL2 inhibition an situ mouse model suppressed growth, decreased MDSCs Tregs, fostered immune suppression. The study results highlight role cell-cell crosstalk during identification pivotal signaling factors driving relapse for development novel therapies.

Language: Английский

Citations

25
Cancer-Associated Fibroblast Heterogeneity and Its Influence on the Extracellular Matrix and the Tumor Microenvironment DOI Open Access
Karl Knipper, Su Ir Lyu, Alexander Quaas

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(17), P. 13482 - 13482

Published: Aug. 30, 2023

The tumor microenvironment comprises multiple cell types, like cancer cells, endothelial fibroblasts, and immune cells. In recent years, there have been massive research efforts focusing not only on but also other types of the microenvironment, thereby aiming to expand determine novel treatment options. Fibroblasts represent a heterogenous family consisting numerous subtypes, which can alter fractions, facilitate or inhibit growth, build pre-metastatic niches, stabilize vessels. These effects be achieved through cell–cell interactions, form extracellular matrix, via secretion cytokines chemokines. pro- antitumorigenic fibroblast phenotypes show variability among different entities, intraindividual sites, including primary tumors metastatic lesions. Commonly prescribed for arterial hypertension, inhibitors renin–angiotensin system recently described as having an inhibitory effect fibroblasts. This inhibition leads modified fractions increased tissue stiffness, contributing overcoming therapy resistance ultimately inhibiting growth. However, it is important note that fibroblasts opposite effect, potentially resulting in We aim summarize latest state regarding heterogeneity its intricate impact matrix. Specifically, we focus highlighting advancements comprehension options within this context.

Language: Английский

Citations

23
Single-cell chemokine receptor profiles delineate the immune contexture of tertiary lymphoid structures in head and neck squamous cell carcinoma DOI
Boxin Zhang, Hao Li, Yuan‐Tong Liu

et al.

Cancer Letters, Journal Year: 2023, Volume and Issue: 558, P. 216105 - 216105

Published: Feb. 24, 2023

Language: Английский

Citations

18
ETS1-mediated Regulation of SOAT1 Enhances the Malignant Phenotype of Oral Squamous Cell Carcinoma and Induces Tumor-associated Macrophages M2-like Polarization DOI Creative Commons

Yueying Liu,

Li Shen,

Yi Li

et al.

International Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 20(9), P. 3372 - 3392

Published: Jan. 1, 2024

Oral squamous cell carcinoma (OSCC) is an aggressive cancer that poses a substantial threat to human life and quality of globally. Lipid metabolism reprogramming significantly influences tumor development, affecting not only cells but also tumor-associated macrophages (TAMs) infiltration.

Language: Английский

Citations

6
Tumor-colonized Streptococcus mutans metabolically reprograms tumor microenvironment and promotes oral squamous cell carcinoma DOI Creative Commons
Jiaying Zhou, Zixuan Hu, Lei Wang

et al.

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: Oct. 5, 2024

Language: Английский

Citations

6
Mannose-doped metal-organic frameworks induce tumor cell pyroptosis via the PERK pathway DOI Creative Commons
Nianqiang Jin,

Binhang Wang,

Xinyao Liu

et al.

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Nov. 15, 2023

The implementation of pyroptosis exhibits significant potential as a tactic to enhance tumor immune microenvironments. Previous applications inducers have encountered various limitations, such the development drug resistance, manifestation toxic side effects, and deficiency in targeting capabilities. As result, there is growing demand for therapeutic molecules that can overcome these obstacles. Therefore, objective this study develop multifunctional nanospheres addresses challenges by enabling high-precision cells inducing effective pyroptosis.We prepared mannose-modified MOF called mannose-doped Fe3O4@NH2-MIL-100 (M-FNM). M-FNM could enter CAL27 through MR-mediated endocytosis, which caused increase level intracellular ROS. This subsequently triggered ER stress activated PERK-eIF2α-ATF4-CHOP signaling pathway. CHOP then mediated downstream cascade Caspase-1, pyroptosis. In vivo experiments, demonstrated excellent ability exhibited anti-tumor effects. Additionally, reshaped microenvironment promoting infiltration cells, primarily T lymphocytes.M-FNM significantly decreased growth. novel approach induce using may offer new avenues immunotherapies against cancer.

Language: Английский

Citations

14
Emerging strategies to investigate the biology of early cancer DOI
Ran Zhou, Xiwen Tang, Yuan Wang

et al.

Nature reviews. Cancer, Journal Year: 2024, Volume and Issue: 24(12), P. 850 - 866

Published: Oct. 21, 2024

Language: Английский

Citations

5
Tryptophan 2,3‐dioxygenase‐positive matrix fibroblasts fuel breast cancer lung metastasis via kynurenine‐mediated ferroptosis resistance of metastatic cells and T cell dysfunction DOI Creative Commons
Yongcan Liu, Shan-Chun Chen,

Xueying Wan

et al.

Cancer Communications, Journal Year: 2024, Volume and Issue: 44(11), P. 1261 - 1286

Published: Sept. 2, 2024

Abstract Background Tumor metastasis is a major threat to cancer patient survival. The organ‐specific niche plays pivotal role in tumor organotropic metastasis. Fibroblasts serve as vital component of the metastatic microenvironment, but how heterogeneous metastasis‐associated fibroblasts (MAFs) promote poorly characterized. Here, we aimed decipher heterogeneity MAFs and elucidate distinct roles these pulmonary formation breast cancer. Methods Mouse models were established using an vivo selection method repeated injections cells purified from mouse lung. Single‐cell RNA‐sequencing (scRNA‐seq) was employed investigate MAFs. Transgenic mice used examine contribution tryptophan 2,3‐dioxygenase‐positive matrix (TDO2 + MFs) lung Results We uncovered 3 subtypes their transcriptome profiles changed dynamically evolved. As predominant subtype, MFs exclusively marked by platelet‐derived growth factor receptor alpha (PDGFRA) mainly located on edge metastasis, T enriched around MFs. Notably, high MF signatures significantly associated with poor survival patients. Lung metastases markedly diminished, suppression dramatically attenuated MF‐depleted experimental models. found that TDO2 controlled producing kynurenine (KYN), which upregulated ferritin heavy chain 1 (FTH1) level disseminated (DTCs), enabling DTCs resist ferroptosis. Moreover, MF‐secreted chemokines C‐C motif chemokine ligand 8 (CCL8) 11 (CCL11) recruited cells. MF‐derived KYN induced cell dysfunction. Conditional knockout Tdo2 diminished enhanced immune activation. Conclusions Our study reveals crucial promoting DTCs’ evasion niche. It suggests targeting metabolism lung‐specific stromal may be effective treatment strategy for patients

Language: Английский

Citations

4