Cross one single body 49 tissues single-cell transcriptome reveals detailed macrophage heterogeneity during pig pregnancy DOI Creative Commons
Xiaoyun Chen, Chih‐Huang Lai, Liping Cai

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 2, 2025

Pregnancy involves complex physiological adaptations across maternal organs and the immune system to support fetal development. Macrophages play a dual role during pregnancy: defending against pathogens supporting tissue adaptation. However, comprehensive in-depth studies of cross-tissue transcriptional heterogeneity macrophages healthy pregnancy at single-cell level remain elusive. We performed RNA sequencing (scRNA-seq) profile from pregnant pig 49 tissues. Immunofluorescence was verify specific expression transcription factors. In this study, we generated macrophage atlas containing 114,881 tissues/organs within one single pig, identified 33 subtypes, revealed extensive tissue-specific diversity. observed significant subtypes five different anatomical sites adipose tissue. Notably, Mφ MARCO+ subtype, primarily derived mesenteric tissue, showed higher activity in pattern recognition receptor signaling pathways compared other tissues, including fat depots. Cross-tissue analysis distinct patterns factors, cytokines, cell surface receptors, factor PLSCR1, specifically expressed lung verified by immunofluorescence. Cross-species unveiled conservation among pigs, humans, mice. constructed multiple-tissue transcriptome revealing their molecular differences commonalities tissues species. Our study provides valuable resource for understanding diversity pigs.

Language: Английский

Microbiota and Immunity during Respiratory Infections: Lung and Gut Affair DOI Open Access
Veronica Marrella,

Federico Nicchiotti,

Barbara Cassani

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 4051 - 4051

Published: April 5, 2024

Bacterial and viral respiratory tract infections are the most common infectious diseases, leading to worldwide morbidity mortality. In past 10 years, importance of lung microbiota emerged in context pulmonary although mechanisms by which it impacts intestinal environment have not yet been fully identified. On contrary, gut microbial dysbiosis is associated with disease etiology or/and development lung. this review, we present an overview microbiome modifications occurring during infections, namely, reduced community diversity increased burden, downstream consequences on host–pathogen interaction, inflammatory signals, cytokines production, turn affecting progression outcome. Particularly, focus role gut–lung bidirectional communication shaping inflammation immunity context, resuming both animal human studies. Moreover, discuss challenges possibilities related novel microbial-based (probiotics dietary supplementation) microbial-targeted therapies (antibacterial monoclonal antibodies bacteriophages), aimed remodel composition resident communities restore health. Finally, propose outlook some relevant questions field be answered future research, may translational relevance for prevention control infections.

Language: Английский

Citations

18
The guardians of pulmonary harmony: alveolar macrophages orchestrating the symphony of lung inflammation and tissue homeostasis DOI Creative Commons
Learta Pervizaj-Oruqaj, Maximiliano Ruben Ferrero, Ulrich Matt

et al.

European Respiratory Review, Journal Year: 2024, Volume and Issue: 33(172), P. 230263 - 230263

Published: April 30, 2024

Recent breakthroughs in single-cell sequencing, advancements cellular and tissue imaging techniques, innovations cell lineage tracing, insights into the epigenome collectively illuminate enigmatic landscape of alveolar macrophages lung under homeostasis disease conditions. Our current knowledge reveals functional diversity within respiratory system, emphasising their remarkable adaptability. By synthesising from classical developmental biology studies, we provide a comprehensive perspective on macrophage plasticity. This includes an examination ontology-related features, role maintaining steady-state conditions distinct contribution bone marrow-derived (BMDMs) promoting regeneration restoring system response to injuries. Elucidating signalling pathways inflammatory conditions, impact various triggers tissue-resident (TR-AMs), as well recruitment polarisation originating marrow, presents opportunity propose innovative therapeutic approaches aimed at modulating equilibrium between phenotypes induce programmes associated with pro-regenerative or phenotype BMDMs TR-AMs. This, turn, can lead amelioration outcomes attenuation detrimental inflammation. review comprehensively addresses pivotal orchestration inflammation resolution phases after injury, ageing-related shifts influence clonal haematopoiesis indeterminate potential mutations macrophages, exploring altered transcriptional profiles, implications for homeostasis.

Language: Английский

Citations

17
Development of an Inhalable DNA Tetrahedron MicroRNA Sponge DOI
Lan Yao, Geru Zhang, Yun Wang

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 22, 2024

Abstract In designing aerosolized drugs, the challenge lies in achieving optimal penetration and retention. Existing delivery systems prioritize larger particles for prolonged intrapulmonary retention, compromising speed. Conversely, smaller nanoparticles face rapid clearance limited RNA sponges featuring multiple microRNA binding sites exhibit promising potential gene expression regulation. However, complex structure of frequently utilized cyclic sponge impedes cellular uptake, restricting its application. This study proposes an innovative approach using a compact tetrahedral framework nucleic acid to construct inhalable sponge. Distinguished by simplified structure, this ensures effective inhibition, tissue penetration, residency through prompt endocytosis. Validated acute lung inflammation models, demonstrates swift restoration local immune homeostasis. design addresses critical need aerosol vehicles that balance efficient sustained offering solution

Language: Английский

Citations

15
Exocarpium Citri Grandis ameliorates LPS-induced acute lung injury by suppressing inflammation, NLRP3 inflammasome, and ferroptosis DOI

Zaibin Xu,

Jiayu Li,

Kaili Zhou

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 329, P. 118162 - 118162

Published: April 7, 2024

Language: Английский

Citations

8
Modulation of alveolar macrophage and mitochondrial fitness by medicinal plant-derived nanovesicles to mitigate acute lung injury and viral pneumonia DOI Creative Commons

Lusha Ye,

Yanan Gao, Simon Wing Fai Mok

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: April 18, 2024

Acute lung injury (ALI) is generally caused by severe respiratory infection and characterized overexuberant inflammatory responses inefficient pathogens-containing, the two major processes wherein alveolar macrophages (AMs) play a central role. Dysfunctional mitochondria have been linked with distorted hence disorders, but few treatments are currently available to correct these defects. Plant-derive nanovesicles gained significant attention because of their therapeutic potential, targeting cells underlying mechanism remain elusive. We herein prepared from Artemisia annua, well-known medicinal plant multiple attributes involving anti-inflammatory, anti-infection, metabolism-regulating properties. By applying three mice models acute bacterial endotoxin, influenza A virus (IAV) SARS-CoV-2 pseudovirus respectively, we showed that Artemisia-derived (ADNVs) substantially alleviated immunopathology raised survival rate challenged mice. Macrophage depletion adoptive transfer studies confirmed requirement AMs for ADNVs effects. identified gamma-aminobutyric acid (GABA) enclosed in vesicles molecular effector mediating regulatory roles ADNVs. Specifically, GABA acts on through receptors, promoting mitochondrial gene programming bioenergy generation, reducing oxidative stress signals, thereby enhancing adaptability inflammation resolution. Collectively, this study identifies promising nanotherapeutics alleviating pathology, elucidates whereby canonical neurotransmitter modifies resume tissue homeostasis, which may broader implications treating critical pulmonary diseases such as COVID-19.

Language: Английский

Citations

8
Lipid-Laden Macrophages in Pulmonary Diseases DOI Creative Commons
Yin Zhu,

Dooyoung Choi,

Payaningal R. Somanath

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(11), P. 889 - 889

Published: May 22, 2024

Pulmonary surfactants play a crucial role in managing lung lipid metabolism, and dysregulation of this process is evident various diseases. Alternations metabolism lead to pulmonary surfactant damage, resulting hyperlipidemia response injury. Lung macrophages are responsible for recycling damaged droplets maintain homeostasis. The inflammatory triggered by external stimuli such as cigarette smoke, bleomycin, bacteria can interfere with process, the formation lipid-laden (LLMs), also known foamy macrophages. Recent studies have highlighted potential significance LLM range Furthermore, growing evidence suggests that LLMs present patients suffering from conditions. In review, we summarize essential metabolic signaling pathways driving chronic obstructive disease, fibrosis, tuberculosis, acute

Language: Английский

Citations

8
Bacteriophage derived dsRNA induces polarized activation of alveolar macrophages from Balb/c and C57Bl/6 mice in vitro in sex- and age-dependent manner DOI
R. Dovhyi,

A. Dvukhriadkina,

K. Ostrovska

et al.

Cellular Immunology, Journal Year: 2025, Volume and Issue: 408, P. 104916 - 104916

Published: Jan. 4, 2025

Language: Английский

Citations

1
Alveolar Macrophages in Viral Respiratory Infections: Sentinels and Saboteurs of Lung Defense DOI Open Access

Pauline Pöpperl,

Melanie Stoff, Andreas Beineke

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 407 - 407

Published: Jan. 5, 2025

Respiratory viral infections continue to cause pandemic and epidemic outbreaks in humans animals. Under steady-state conditions, alveolar macrophages (AlvMϕ) fulfill a multitude of tasks order maintain tissue homeostasis. Due their anatomic localization within the deep lung, AlvMϕ are prone detect react inhaled viruses thus play role early pathogenesis several respiratory infections. Here, detection pathogens causes diverse antiviral proinflammatory reactions. This fact not only makes them promising research targets, but also suggests as potential targets for therapeutic prophylactic approaches. review aims give comprehensive overview current knowledge about

Language: Английский

Citations

1
Biomimetic MOF Nanocarrier‐Mediated Synergistic Delivery of Mitochondria and Anti‐Inflammatory miRNA to Alleviate Acute Lung Injury DOI Creative Commons
Xin Shou, Changjiang Chen,

Hangjie Ying

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Abstract Acute lung injury (ALI) is a clinically critical disease characterized by overwhelming inflammatory response and significant tissue damage with no specific treatment available currently. As key player in the pathogenesis of ALI, macrophages are aberrantly activated polarize toward pro‐inflammatory phenotypes, leading to overzealous inflammation injury. Mitochondria recognized as crucial signaling hub governing macrophage function polarization, deregulation which causatively related defective metabolism macrophages, deregulated inflammation, hence ALI. Herein, an inflammation‐responsive, biomimetic metal‐organic framework (MOF) nanoplatform, termed a127/mito@ZIF@Ma developed, sophistically designed for synergistic delivery macrophage‐derived mitochondria anti‐inflammatory miRNA‐127 antagonist resume pulmonary homeostasis alleviate Notably, membrane encapsulation conferred MOF enhanced transport efficacy both vitro vivo. Therefore, administration nanoparticles accordingly profound protection mice against induced either bacterial or viral infection unnoticeable toxicity. The study thus devises novel MOF‐based nanosystem that integrates transplantation miRNA therapeutics, may open new avenue treating ALI relevant diseases.

Language: Английский

Citations

1
Regulation of macrophage activation by lactylation in lung disease DOI Creative Commons
Yungeng Wei, Hua Guo,

Shixing Chen

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: July 4, 2024

Lactylation is a process where lactate, cellular metabolism byproduct, added to proteins, altering their functions. In the realm of macrophage activation, lactylation impacts inflammatory response and immune regulation. Understanding effects on activation vital in lung diseases, as abnormal function are pivotal conditions like pneumonia, pulmonary fibrosis, COPD, cancer. This review explores concept lactylation, its regulation recent research progress diseases. It offers new insights into disease pathogenesis potential therapeutic targets.

Language: Английский

Citations

7