The Genetics of Sleep in Drosophila

Springer eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 7 - 56

Published: Jan. 1, 2024

Language: Английский

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Chronic mitochondrial fragmentation elicits a neuroprotective Warburg-like effect in Drosophila neurons

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Abstract Mitochondrial fission and fusion are dynamic important cellular processes, but the roles of these two very different mitochondrial forms – predominantly spherical tubular - not well-characterized in neurons animals especially aging neurons. This is because long-lived dynamics associated with neurodegenerative diseases. We used here an efficient cell type-specific CRISPR approach to knockout key fission-fusion genes disrupt within inessential clock Drosophila . Surprisingly, much more than for maintaining long-term neuronal function. Neurons survive chronic fragmentation due loss by triggering a cancer-like transcriptomic response. Warburg effect includes ATF4-mediated upregulation aerobic glycolysis gene Lactate dehydrogenase ( Ldh ), LDH essential prevent neurodegeneration deficient Opa1 These results others provide insights into intersection metabolism, neurodegeneration.

Language: Английский

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Circadian Influences on Brain Lipid Metabolism and Neurodegenerative Diseases

Metabolites, Journal Year: 2024, Volume and Issue: 14(12), P. 723 - 723

Published: Dec. 22, 2024

Circadian rhythms are intrinsic, 24 h cycles that regulate key physiological, mental, and behavioral processes, including sleep–wake cycles, hormone secretion, metabolism. These controlled by the brain’s suprachiasmatic nucleus, which synchronizes with environmental signals, such as light temperature, consequently maintains alignment day–night cycle. Molecular feedback loops, driven core circadian “clock genes”, Clock, Bmal1, Per, Cry, essential for rhythmic gene expression; disruptions in these loops associated various health issues. Dysregulated lipid metabolism brain has been implicated pathogenesis of neurological disorders contributing to oxidative stress, neuroinflammation, synaptic dysfunction, observed conditions Alzheimer’s Parkinson’s diseases. Disruptions expression have shown perturb regulatory mechanisms brain, thereby triggering neuroinflammatory responses damage. This review synthesizes current insights into interconnections between metabolism, a focus on their roles disease. It further examines how desynchronization genes affects explores potential through disrupted signaling might contribute pathophysiology neurodegenerative disorders.

Language: Английский

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A new player in circadian networks: Role of electrical synapses in regulating functions of the circadian clock

Frontiers in Physiology, Journal Year: 2022, Volume and Issue: 13

Published: Nov. 3, 2022

Several studies have indicated that coherent circadian rhythms in behaviour can be manifested only when the underlying oscillators function as a well-coupled network. The current literature suggests pacemaker neuronal networks rely heavily on communication mediated by chemical synapses comprising neuropeptides and neurotransmitters to regulate several behaviours physiological processes. It has become increasingly clear closely interact with electrical together of most organisms. However, there are few which examined role here, we review our understanding gap junction proteins various model systems. We describe general mechanisms neural networks, their interactions neuromodulators contributions regulation rhythms. also discuss methods available characterize functional these potential directions remain explored understand roles this relatively understudied mechanism modulating behaviour.

Language: Английский

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Connectomic Analysis of the Drosophila Lateral Neuron Clock Cells Reveals the Synaptic Basis of Functional Pacemaker Classes

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: March 4, 2022

Abstract The circadian clock orchestrates daily changes in physiology and behavior to ensure internal temporal order optimal timing across the day. In animals, a central brain coordinates rhythms throughout body is characterized by remarkable robustness that depends on synaptic connections between constituent neurons. neuron network of Drosophila , which shares motifs with networks mammalian yet built many fewer neurons, offers powerful model for understanding properties timekeeping. Here we report an assessment connectivity within network, focusing critical lateral (LN) classes. Our results reveal previously identified anatomical functional subclasses LNs represent distinct connectomic types. Moreover, identify small number cell subtypes representing highly synaptically coupled nodes network. This suggests neurons lacking molecular timekeeping likely play integral roles To our knowledge, this represents first comprehensive analysis neuronal

Language: Английский

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