Oxidoreductases and Reactive Oxygen Species in Conversion of Lignocellulosic Biomass

Microbiology and Molecular Biology Reviews, Journal Year: 2018, Volume and Issue: 82(4)

Published: Sept. 25, 2018

Biomass constitutes an appealing alternative to fossil resources for the production of materials and energy. The abundance attractiveness vegetal biomass come along with challenges pertaining intricacy its structure, evolved during billions years face resist abiotic biotic attacks. To achieve daunting goal plant cell wall decomposition, microorganisms have developed many (enzymatic) strategies, from which we seek inspiration develop biotechnological processes. A major breakthrough in field has been discovery enzymes today known as lytic polysaccharide monooxygenases (LPMOs), which, by catalyzing oxidative cleavage recalcitrant polysaccharides, allow canonical hydrolytic depolymerize more efficiently. Very recently, it shown that LPMOs are not classical they can also use hydrogen peroxide (H2O2) oxidant. This calls a revision our understanding how lignocellulolytic connected since H2O2 is produced used several them. first part this review dedicated LPMO paradigm, describing knowns, unknowns, uncertainties. We then present different redox systems, enzymatic or not, depend on fluxes reactive oxygen species (ROS). Based assessment these putatively interconnected suggest fine-tuning levels proximity between sites consumption important fungal conversion. In last review, discuss evolving processes involved depolymerization may translate into industrial applications.

Language: Английский

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The influence of feedstock characteristics on enzyme production in Trichoderma reesei: a review on productivity, gene regulation and secretion profiles

Biotechnology for Biofuels, Journal Year: 2019, Volume and Issue: 12(1)

Published: Oct. 8, 2019

Abstract Biorefineries, designed for the production of lignocellulose-based chemicals and fuels, are receiving increasing attention from public, governments, industries. A major obstacle biorefineries to advance commercial scale is high cost enzymes required derive fermentable sugars feedstock used. As summarized in this review, techno-economic studies suggest co-localization integration enzyme manufacturing with cellulosic biorefinery as most promising alternative alleviate problem. Thus, cultivation Trichoderma reesei , principal producer lignocellulolytic enzymes, on lignocellulosic biomass processed on-site can reduce manufacturing. Further, due a complex gene regulation machinery, fungus adjust expression towards characteristics feedstock, hydrolytic efficiency produced cocktail. Despite extensive research over decades, underlying regulatory mechanisms not fully elucidated. One aspect that has received relatively little literature influence substrate, i.e., its chemical physical composition, mixture. Considering dependent efficient enzymatic degradation lignocellulose continuous supply carbon energy, relationship between secretome composition be expected. The aim review was systematically collect, appraise, aggregate data integrate results analyzing by T. insoluble model substrates biomass. show there direct effect substrate’s complexity (rated structure, lignin–carbohydrate complex, recalcitrance saccharification) titers specific activities secretome. It further shows process-related factors, such substrate loading set-up, targets yields. transcriptome supports proposed substrate-related factors enzymes. This provides insights into interrelation which may help integrated substrate-specific cocktails at scale.

Language: Английский

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Fungal strain improvement for efficient cellulase production and lignocellulosic biorefinery: Current status and future prospects

Bioresource Technology, Journal Year: 2023, Volume and Issue: 385, P. 129449 - 129449

Published: July 4, 2023

Language: Английский

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Transcriptome and secretome analysis of Aspergillus fumigatus in the presence of sugarcane bagasse

BMC Genomics, Journal Year: 2018, Volume and Issue: 19(1)

Published: April 3, 2018

Sugarcane bagasse has been proposed as a lignocellulosic residue for second-generation ethanol (2G) produced by breaking down biomass into fermentable sugars. The enzymatic cocktails degradation are mostly fungi, but low cost and high efficiency can consolidate 2G technologies. A. fumigatus plays an important role in plant capabilities recycling. To gain more insight the divergence gene expression during steam-exploded (SEB) breakdown, this study profiled transcriptome of RNA sequencing to compare transcriptional profiles grown on media containing SEB or fructose sole carbon source. Secretome analysis was also performed using SDS-PAGE LC-MS/MS. maximum activities cellulases (0.032 U mL-1), endo-1,4-β--xylanase (10.82 mL-1) endo-1,3-β glucanases (0.77 showed that functional CAZymes (carbohydrate-active enzymes) were secreted culture conditions. Correlations between secretome data identified several fumigatus. Particular attention given related lignocellulose sugar transporters. Genes encoding glycoside hydrolase classes commonly expressed breakdown cellulose, such GH-5, 6, 7, 43, 45, hemicellulose, GH-2, 10, 11, 30, found be highly Lytic polysaccharide monooxygenases (LPMO) classified auxiliary activity families AA9 (GH61), CE (1, 4, 8, 15, 16), PL 3, 20) GT 2, 20, 35, 48) differentially condition. Similarly, most enzymes degradation, including endoxylanases, xyloglucanases, β-xylosidases, LPMOs, α-arabinofuranosidases, cellobiohydrolases, endoglucanases β-glucosidases, secretome. This is first report experiment Aspergillus pretreated sugarcane bagasse. results suggest strain employs strategies complex process. It possible identify set genes proteins might applied biotechnology fields. knowledge exploited improvement production rational design cocktails.

Language: Английский

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Identification of Mycoparasitism-Related Genes against the Phytopathogen Botrytis cinerea via Transcriptome Analysis of Trichoderma harzianum T4

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(3), P. 324 - 324

Published: March 6, 2023

Trichoderma harzianum is a well-known biological control agent (BCA) that effective against variety of plant pathogens. In previous studies, we found T. T4 could effectively the gray mold in tomatoes caused by Botrytis cinerea. However, research on its biocontrol mechanism not comprehensive, particularly regarding mycoparasitism. this study, order to further investigate mycoparasitism T4, transcriptomic sequencing and real-time fluorescence quantitative PCR (RT-qPCR) were used identify differentially expressed genes (DEGs) at 12, 24, 48 72 h growth cell wall B. cinerea (BCCW) or sucrose medium. A total 2871 DEGs 2148 novel detected using transcriptome sequencing. Through GO KEGG enrichment analysis, identified associated with specific time periods, such as encoding kinases, signal transduction proteins, carbohydrate active enzymes, hydrolytic transporters, antioxidant secondary metabolite synthesis, resistance detoxification extended hyphal longevity. To validate data, RT-qCPR was performed samples. The RT-qPCR results show expression trend consistent RNA-Seq data. screened mycoparasitism, dual-culture antagonism test showed 15 24 upregulated during after contact between (the same BCCW), which confirmed these involved T4. conclusion, data provided study will only improve annotation information gene models genome, but also provide important process can help us understand thus providing potential molecular target for agent.

Language: Английский

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Exploring Trichoderma and Aspergillus secretomes: Proteomics approaches for the identification of enzymes of biotechnological interest

Enzyme and Microbial Technology, Journal Year: 2017, Volume and Issue: 109, P. 1 - 10

Published: Aug. 25, 2017

Language: Английский

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Sulfadiazine biodegradation by Phanerochaete chrysosporium: Mechanism and degradation product identification

Chemosphere, Journal Year: 2019, Volume and Issue: 237, P. 124418 - 124418

Published: July 25, 2019

Language: Английский

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New Genomic Approaches to Enhance Biomass Degradation by the Industrial FungusTrichoderma reesei

International Journal of Genomics, Journal Year: 2018, Volume and Issue: 2018, P. 1 - 17

Published: Sept. 24, 2018

The filamentous fungi Trichoderma reesei is one of the most well-studied cellulolytic microorganisms. It important fungus for industrial production enzymes to biomass deconstruction being widely used in biotechnology industry, mainly biofuels. Here, we performed an analytic review holocellulolytic system presented by T. as well transcriptional and signaling mechanisms involved with holocellulase expression this fungus. We also discuss new perspectives about control secretion cellulase based on RNA-seq functional characterization data growth different carbon sources, which comprise glucose, cellulose, sophorose, sugarcane bagasse.

Language: Английский

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The enzyme interactome concept in filamentous fungi linked to biomass valorization

Bioresource Technology, Journal Year: 2021, Volume and Issue: 344, P. 126200 - 126200

Published: Oct. 26, 2021

Language: Английский

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Bacterial volatile organic compounds induce adverse ultrastructural changes and DNA damage to the sugarcane pathogenic fungus Thielaviopsis ethacetica

Environmental Microbiology, Journal Year: 2022, Volume and Issue: 24(3), P. 1430 - 1453

Published: Jan. 7, 2022

Summary Due to an increasing demand for sustainable agricultural practices, the adoption of microbial volatile organic compounds (VOCs) as antagonists against phytopathogens has emerged eco‐friendly alternative use agrochemicals. Here, we identified three Pseudomonas strains that were able inhibit, in vitro, up 80% mycelial growth phytopathogenic fungus Thielaviopsis ethacetica , causal agent pineapple sett rot disease sugarcane. Using GC/MS, found these bacteria produced 62 different VOCs, and further functional validation revealed with high antagonistic activity T . Transcriptomic analysis fungal response VOCs indicated metabolites downregulated genes related central metabolism, such those involved carbohydrate metabolism. Interestingly, DNA damage upregulated, micro‐FTIR corroborated our hypothesis triggered damage. Electron microscopy showed critical morphological changes mycelia treated VOCs. Altogether, results hampered could lead cell death. This study represents first demonstration molecular mechanisms antagonism sugarcane by reinforces can be a phytopathogen biocontrol.

Language: Английский

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