Mycoparasitic transcription factor 1 (BcMTF1) participates in the Botrytis cinerea response against Trichoderma atroviride

iScience, Journal Year: 2025, Volume and Issue: 28(2), P. 111783 - 111783

Published: Jan. 10, 2025

Botrytis cinerea is a phytopathogenic fungus. Traditional control using fungicides has faced challenges, prompting the exploration of sustainable alternatives such as biocontrol. Trichoderma atroviride, promising biocontroller, well-known for its mycoparasitism. However, molecular processes involved in this fungal-fungal interaction, particularly regarding defense mechanisms pathogen, have yet to be deeply studied. Here, we investigated transcriptional responses B. T. atroviride. We focused on four transcription factors (TFs) differentially expressed during interaction with biocontroller. Mutants lacking these TFs exhibit increased sensitivity Bcin07g06800 (BcMTF1) loss-of-function mutant being most susceptible. Genes predicted regulated by mycoparasitic factor 1 were interaction. BcMTF1 influences cinerea's resistance mycoparasitism regulating expression genes potentially fungal against Trichoderma. The findings provide insights into underlying interactions.

Language: Английский

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Genome and transcriptome sequencing of Trichoderma harzianum T4, an important biocontrol fungus of Rhizoctonia solani, reveals genes related to mycoparasitism

Canadian Journal of Microbiology, Journal Year: 2024, Volume and Issue: 70(3), P. 86 - 101

Published: Feb. 5, 2024

is a well-known biological control strain and mycoparasite of

Language: Английский

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Unravelling the transcriptomic dynamics of Hyphopichia pseudoburtonii in co-culture with Botrytis cinerea

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0316713 - e0316713

Published: Jan. 14, 2025

Hyphopichia pseudoburtonii , is emerging as a potential biocontrol agent against various phytopathogens. These traits have been attributed to the production of antifungal compounds in presence target pathogens. However, broad molecular mechanisms involved activity are not yet understood. This study employed RNA sequencing assess temporal changes H . Y963 gene expression patterns when co-cultivated with Botrytis cinerea Genes differentially expressed co-culture B compared monoculture were evaluated after 24, 48, and 120 h growth. Up-regulation genes encoding major core histones (H2A, H3, H4) ribosomes first 24 suggested an abundance cells S phase cell cycle. At 48 h, up-regulated highlight mitotic cycle induction filamentous growth, while later stages, up-regulation high affinity transporters sugars, copper iron, well those retention utilization siderophore-iron was evident. Altogether, data allude competition for space nutrients key activated research offers new insights into transcriptomic response illuminates adaptive strategies behind its activity.

Language: Английский

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From Field to Fork: the benefits of Trichoderma spp. in food quality and safety

Current Opinion in Food Science, Journal Year: 2025, Volume and Issue: unknown, P. 101286 - 101286

Published: Feb. 1, 2025

Language: Английский

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Relevance of Mushrooms for Biological Control of Diverse Biotic Agent Mitigating Against Agricultural Crops

Published: March 20, 2025

Language: Английский

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Potencial de Trichoderma spp como agente antagonista de Phytophthora palmivora en plántulas de cacao variedad CCN-51

Horizon Nexus Journal., Journal Year: 2025, Volume and Issue: 3(2), P. 18 - 35

Published: April 30, 2025

Phytophthora palmivora es un patógeno devastador en cultivos de cacao, que causa enfermedades como la podredumbre vaina y pudrición raíz, afectando significativamente productividad calidad los cultivos. Dado el control químico presenta desafíos debido a resistencia del sus efectos ambientales, biológico mediante hongos Trichoderma harzianum se una alternativa prometedora. Este estudio evaluó potencial tres cepas (TCH-01, TCH-22, TCH-09) agentes biocontroladores P. plántulas cacao variedad CCN-51. Se realizaron evaluaciones crecimiento radial las cepas, inhibición esporulación in vitro, así incidencia severidad enfermedad tratadas. Los resultados mostraron TCH-01 fue cepa más eficaz, logrando 80% 96%, lo superior otras cepas. En también presentó menor (10%) (20%) enfermedad, comparación con controles. Estos demuestran tiene alto para ser utilizado biocontrolador palmivora, contribuyendo estrategias manejo sostenibles reduciendo dependencia tratamientos químicos cultivo cacao.

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Potential of Trichoderma virens HZA14 in Controlling Verticillium Wilt Disease of Eggplant and Analysis of Its Genes Responsible for Microsclerotial Degradation

Plants, Journal Year: 2023, Volume and Issue: 12(21), P. 3761 - 3761

Published: Nov. 3, 2023

Verticillium dahliae is a soilborne fungal pathogen that causes vascular wilt diseases in wide range of economically important crops, including eggplant. Trichoderma spp. are effective biological control agents suppress plant pathogens through variety mechanisms, mycoparasitism. However, the molecular mechanisms mycoparasitism degradation microsclerotia V. not yet fully understood. In this study, ability 15 isolates to degrade was evaluated using dual culture method. After days, isolate HZA14 showed greatest potential for microsclerotial degradation. The filtrate also significantly inhibited mycelial growth and conidia germination at different dilutions. Moreover, study T. virens produced siderophores indole-3-acetic acid (IAA). disease tests, reduced severity eggplant seedlings by up 2.77%, resulting efficacy 96.59% 30 days after inoculation. Additionally, inoculation with an increased stem root length fresh dry weight, demonstrating promotion efficacy. To further investigate mechanism HZA14, transcriptomics sequencing real-time fluorescence quantitative PCR (RT-qPCR) were used identify differentially expressed genes (DEGs) 3, 6, 9, 12, interaction dahliae. contrast group, mycoparasitic process exhibited differential gene expression, 1197, 1758, 1936, 1914 being up-regulated 1191, 1963, 2050, 2114 down-regulated, respectively. Among these genes, enzymes associated microsclerotia, such as endochitinase A1, endo-1,3-beta-glucanase, alpha-N-acetylglucosaminidase, laccase-1, peroxidase predicted based on bioinformatics analysis. RT-qPCR results confirmed RNA-sequencing data, showing expression trend consistent. These provide information understanding integrated management other crops.

Language: Английский

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Molecular techniques for the improvement of microbial biocontrol agents against plant pathogens

Egyptian Journal of Biological Pest Control, Journal Year: 2023, Volume and Issue: 33(1)

Published: Oct. 12, 2023

Abstract Biological control is a sustainable and ecologically effective method for bringing down pest population to an acceptable level. Implementing microbial biocontrol agents (MBCAs) manage plant diseases necessitates the use of environmentally friendly practices that can increase global food production guarantee safety supply. Recent advancements in biotechnology have made it easier find characterize new beneficial microbes as well identify their genetic byproducts. These findings possible clone these plants effort strengthen resistance biotic abiotic stresses. The technological improvements strengthened symbiotic interaction between while also enabling modification processes through which MBCAs exert effects, ultimately enhancing potential managing diseases. genome sequencing has yielded useful information about genomes, helped them efficiently. This article offers thorough summary already existing recent molecular advances used efficiency understand mechanisms various omics technologies. approaches are important assuring security increasing agricultural outputs by minimizing yield loss due

Language: Английский

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The histone deacetylase Hda1 affects oxidative and osmotic stress response as well as mycoparasitic activity and secondary metabolite biosynthesis in Trichoderma atroviride

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(3)

Published: Feb. 9, 2024

The mycoparasitic fungus

Language: Английский

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Synthesis of Extracellular L-lysine-α-oxidase along with Degrading Enzymes by Trichoderma cf. aureoviride Rifai VKM F-4268D: Role in Biocontrol and Systemic Plant Resistance

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(5), P. 323 - 323

Published: April 28, 2024

When cultivating on wheat bran or deactivated fungal mycelium as a model of “natural growth”, the ability Trichoderma to synthesize extracellular L-lysine-α-oxidase (LysO) simultaneously with cell-wall-degrading enzymes (proteases, xylanase, glucanases, chitinases, etc.), responsible for mycoparasitism, was shown. LysO, in turn, causes formation H2O2 and pipecolic acid. These compounds are known be signaling molecules play an important role induction development systemic acquired resistance plants. Antagonistic effects LysO have been demonstrated against phytopathogenic fungi Gram-positive Gram-negative bacteria dose-dependent cell death. The antimicrobial effect decreased presence catalase. generating intracellular ROS also shown both fungi, which led decrease viable cells. results suggest that activity is due two factors: exogenous hydrogen peroxide product enzymatic oxidative deamination L-lysine direct interaction wall micro-organisms. Thus, its own enhances potential producer environment; namely, enzyme complements strategy fungus biocontrol indirectly participates inducing SAR regulating relationship between pathogens

Language: Английский

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