Creative Education Studies, Journal Year: 2024, Volume and Issue: 12(12), P. 261 - 267
Published: Jan. 1, 2024
Language: Английский
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 3901 - 3901
Published: March 31, 2024
Cardiovascular diseases (CVDs) are the leading cause of death and include several vascular cardiac disorders, such as atherosclerosis, coronary artery disease, cardiomyopathies, heart failure. Multiple treatment strategies exist for CVDs, but there is a need regenerative damaged heart. Stem cells broad variety with great differentiation potential that have immunomodulatory properties. studies evaluated efficacy stem in mesenchymal induced pluripotent cell-derived cardiomyocytes. These demonstrated can improve left ventricle ejection fraction, reduce fibrosis, decrease infarct size. Other investigated methods to survival, engraftment, functionality CVDs. The aim present review summarize current evidence on role how their efficacy.
Language: Английский
Citations
11
Cureus, Journal Year: 2024, Volume and Issue: unknown
Published: Feb. 13, 2024
Periodontal disease, characterized by inflammation and infection of the supporting structures teeth, presents a significant challenge in dentistry public health. Current treatment modalities, while effective to some extent, have limitations achieving comprehensive periodontal tissue regeneration. This review explores potential stem cell therapy advancing field Stem cells, including mesenchymal cells (MSCs) induced pluripotent (iPSCs), hold promise due their immunomodulatory effects, differentiation into tissues, paracrine actions. Preclinical studies using various animal models revealed encouraging outcomes, though standardization long-term assessment remain challenges. Clinical trials case demonstrate safety efficacy real-world applications, especially personalized regenerative medicine. Patient selection criteria, ethical considerations, standardized protocols are vital for successful clinical implementation. is poised revolutionize regeneration, offering more effective, patient-tailored treatments addressing systemic health implications disease. transformative approach holds significantly impact practice improve overall well-being individuals affected this prevalent oral concern. Responsible regulatory compliance focus on considerations will be essential as evolves
Language: Английский
Citations
10
ACS Omega, Journal Year: 2025, Volume and Issue: 10(6), P. 5668 - 5685
Published: Feb. 7, 2025
A significant obstacle to the healing process of periodontitis is development bacterial biofilms within periodontal pockets. The efficacy biofilm therapy often hindered by inadequate penetration antibacterial agents and nonspecific targeting bacteria. This study proposes a novel strategy involving utilization pH-sensitive microparticles (MPs) doxycycline (DOX) enhance enable targeted delivery DOX infection sites associated with periodontitis. MPs were developed using double-emulsion technique poly(d,l-lactide-co-glycolide) chitosan in 1:1 ratio. morphology DOX-MP exhibits spherical form particle size 3.54 ± 0.32 μm PDI 0.221 0.02. also had great encapsulation efficiency (69.43% 5.32) drug loading (14.81% 1.32) regulated release kinetics accelerated rates under low-pH conditions. antimicrobial activity was evaluated against Escherichia coli Staphylococcus aureus, results indicated absence any viable colonies after 18 h at twice minimum inhibitory concentration value. Hydrogel-based deliver pocket site for ease use. In situ hydrogels used Pluronic F127 F68 as main polymer composition hydroxypropyl methylcellulose adhesion polymer. formulation exhibited liquid state room temperature (25 °C) but went through gelation 36 °C. good mucoadhesive characteristics (42.65 3.53 dyn/cm2) permeation acidic pH Mueller-Hinton Broth media addition E. S. aureus Ex vivo significantly reduced microbial count, quantity, metabolic activity, confirming desired effect. Hence, free drugs DOX-MPs did not exhibit notable dissimilarity, showing that integrating into matrix hindering its efficacy.
Language: Английский
Citations
1
Dentistry Journal, Journal Year: 2025, Volume and Issue: 13(3), P. 106 - 106
Published: Feb. 27, 2025
This review highlights the key molecular and cellular mechanisms contributing to aging, such as DNA damage, mitochondrial dysfunction, telomere shortening, protein defective autophagy. These biological are involved in various oral health conditions prevalent elderly, including periodontal disease, cancer, xerostomia, dental caries, temporomandibular joint disorders. Exosomes generated by mesenchymal stem cells possess substantial therapeutic potential. exosomes nanosized extracellular vesicles derived from essential intercellular communication tissue homeostasis. The exosome-based therapies proved superior traditional cell-based approaches, due lower immunogenicity, ease of storage, avoidance complications associated with cell transplantation. Furthermore, diagnostic potential non-invasive biomarkers for aging processes age-related diseases offers insights into disease diagnosis, staging, monitoring. Among challenges future perspectives translating exosome research preclinical studies clinical applications is need standardized procedures fully harness capabilities exosomes.
Language: Английский
Citations
1
Photodiagnosis and Photodynamic Therapy, Journal Year: 2025, Volume and Issue: unknown, P. 104566 - 104566
Published: March 1, 2025
This study assessed the effects of 808 nm diode laser on gene expression receptor activator nuclear factor kappa beta ligand (RANKL) and osteoprotegerin (OPG), key regulators bone remodeling, in exosomes derived from osteoblast-like cells. The cultured MG63 cells were subjected to irradiation at energy densities 3 J/cm², 6 9 along with a control group no intervention. sessions conducted twice, 24-hour interval between them. Next target isolated, mRNA levels RANKL OPG genes using qPCR. level extracted exposed J/cm² was found be significantly elevated compared both J/cm². Conversely, reduced comparison Additionally, also lower than that observed Using an density resulted upregulation exosomal for downregulation RANKL. Photobiomodulation may enhance regeneration via signaling. Considering promising clinical application regeneration, our results highlight potential photobiomodulation manipulate content therapeutic purposes.
Language: Английский
Citations
1
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3225 - 3225
Published: March 30, 2025
Although periodontal disease (PD) is reported to be associated with changes in various genes and proteins both invading bacteria the host, its molecular mechanism of pathogenesis remains unclear. Changes immune inflammatory play a significant role PD pathogenesis. Some reports relate alterations cellular epigenetic patterns characteristics, while several high-throughput analyses indicate thousands differentially methylated patients controls. Furthermore, DNA methylation inflammation-related have been linked efficacy therapy, as demonstrated by findings related cytochrome C oxidase II gene. Distinct mesenchymal stem cells from controls persisted despite reversal phenotypic PD. Methyl groups for are supplied S-adenosylmethionine, which synthesized involvement folate, an essential nutrient known maintaining mitochondrial homeostasis, compromised Folate may benefit through antioxidant action against reactive oxygen nitrogen species that overproduced dysfunctional mitochondria. As such, methylation, dietary quality control interact In this narrative/hypothesis review, we demonstrate how may, turn, improved potentially altering nucleus Therefore, folate-based intervention recommended prevention adjunct therapy. At same time, further research needed on mechanisms beneficial effects folate studies.
Language: Английский
Citations
0
Clinical Oral Investigations, Journal Year: 2025, Volume and Issue: 29(5)
Published: April 23, 2025
Abstract Objective This study investigates the regenerative and protective effects of postbiotics (cell-free supernatant) derived from Lactiplantibacillus plantarum EIR/IF-1 strain on human periodontal ligament mesenchymal stromal cells (hPDL-MSCs). Materials methods hPDL-MSCs were isolated tissues (PDL) wisdom teeth using enzymatic digestion subsequently characterized through immunophenotyping. The effect viability was assessed MTT assay flow cytometry, while their impact cell migration evaluated via scratch assay. Anti-inflammatory investigated lipopolysaccharide (LPS, Porphyromonas gingivalis )-stimulated Enzyme-Linked Immunosorbent Assay (ELISA). Additionally, antioxidant analyzed in hydrogen peroxide (H₂O₂)-induced by measuring reactive oxygen species (ROS) levels cytometry. expression collagen type I ( COL1A1 ) gene further quantitative reverse transcription PCR immunofluorescence staining. Results Treatment with (250 µg/mL) significantly increased capability hPDL-MSCs, enhancing production for PDL repair. 24 h resulted a 54.53 ± 2.01% reduction intracellular ROS compared to untreated H 2 O -induced hPDL-MSCs. Furthermore, decreased pro-inflammatory cytokines (IL-8, IL-6, IL-1β), anti-inflammatory cytokine IL-10 (2.67-fold) LPS-stimulated Conclusion Our findings indicate that exhibit biological activity throughout all stages healing process, beginning modulation inflammatory response LPS stimulation, followed promotion migration, proliferation, synthesis. Given unmet need safe adjuvant therapeutic approaches promote comprehensive regeneration diseases, this presents as promising candidate. Clinical relevance Postbiotics could be integrated into therapies novel bioactive material improve outcomes defects both controlling inflammation stimulating tissue repair processes.
Language: Английский
Citations
0
Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 11
Published: Jan. 12, 2024
Periodontal regeneration involves the composite action of cell, scaffolds and signaling molecules. There are numerous autologous sources regenerative cells which present close to vicinity periodontally debilitated site, primary one being periodontal ligament stem is believed have a key role in regeneration. Various methods can be harnessed optimize enhance potential PDLSCs such as application LASERs. In last few years there been various studies evaluated effect different types LASERs on review summarizes photo-biomodulative activity general its beneficial stimulation PDLSC specifically.
Language: Английский
Citations
3
Dentistry Journal, Journal Year: 2024, Volume and Issue: 12(5), P. 127 - 127
Published: May 7, 2024
Pregnancy induces significant changes in oral health because of hormonal fluctuations, making it a crucial period for preventive measures. Dental stem cells (DSCs), particularly those derived from the dental pulp and periodontal ligaments, offer promising avenues regenerative therapies and, possibly, interventions. While use DSCs already includes various applications dentistry general population, their during pregnancy requires careful consideration. This review explores recent advancements, challenges, prospects using to address issues, possibly pregnancy. Critical aspects responsible pregnant women are discussed, including safety, ethical regulatory frameworks, need interdisciplinary collaborations. We aimed provide comprehensive understanding leveraging improve maternal health.
Language: Английский
Citations
2
Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)
Published: Sept. 27, 2024
Language: Английский
Citations
2