Current Opinion in Chemical Biology, Journal Year: 2022, Volume and Issue: 70, P. 102188 - 102188
Published: Aug. 12, 2022
Language: Английский
Chemical Reviews, Journal Year: 2022, Volume and Issue: 123(5), P. 2349 - 2419
Published: Dec. 13, 2022
Recent advances in synthetic biology and materials science have given rise to a new form of materials, namely engineered living (ELMs), which are composed matter or cell communities embedded self-regenerating matrices their own artificial scaffolds. Like natural such as bone, wood, skin, ELMs, possess the functional capabilities organisms, can grow, self-organize, self-repair when needed. They also spontaneously perform programmed biological functions upon sensing external cues. Currently, ELMs show promise for green energy production, bioremediation, disease treatment, fabricating advanced smart materials. This review first introduces dynamic features systems potential developing novel We then summarize recent research progress on emerging design strategies from both perspectives. Finally, we discuss positive impacts promoting sustainability key future directions.
Language: Английский
Citations
127
Biotechnology Advances, Journal Year: 2024, Volume and Issue: 74, P. 108401 - 108401
Published: June 27, 2024
Metabolic burden is defined by the influence of genetic manipulation and environmental perturbations on distribution cellular resources. The rewiring microbial metabolism for bio-based chemical production often leads to a metabolic burden, followed adverse physiological effects, such as impaired cell growth low product yields. Alleviating imposed undesirable changes has become an increasingly attractive approach constructing robust factories. In this review, we provide brief overview engineering, focusing specifically recent developments strategies diminishing while improving robustness yield. A variety examples are presented showcase promise engineering in facilitating design construction Finally, challenges limitations encountered discussed.
Language: Английский
Citations
29
Journal of Personalized Medicine, Journal Year: 2024, Volume and Issue: 14(2), P. 217 - 217
Published: Feb. 18, 2024
Infectious diseases have long posed a significant threat to global health and require constant innovation in treatment approaches. However, recent groundbreaking research has shed light on previously overlooked player the pathogenesis of disease-the human microbiome. This review article addresses intricate relationship between microbiome infectious unravels its role as crucial mediator host-pathogen interactions. We explore remarkable potential harnessing this dynamic ecosystem develop innovative strategies that could revolutionize management diseases. By exploring latest advances emerging trends, aims provide new perspective combating by targeting
Language: Английский
Citations
27
Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)
Published: July 5, 2022
Synthetic microbial consortia represent a new frontier for synthetic biology given that they can solve more complex problems than monocultures. However, most attempts to co-cultivate these artificial communities fail because of the winner-takes-all in nutrients competition. In soil, multiple species coexist with spatial organization. Inspired by nature, here we show an engineered segregation method assemble stable both flexibility and precision. We create swarmbot (MSBC) encapsulating subpopulations polymeric microcapsules. The crosslinked structure microcapsules fences microbes, but allows transport small molecules proteins. MSBC enables assembly various precise control over subpopulations. These capabilities readily modulate division labor communication. Our work integrates material science offer insights into serve as foundation diverse applications from biomanufacturing photosynthesis.
Language: Английский
Citations
55
Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11
Published: Feb. 10, 2023
Plant Growth Promoting Rhizobacteria (PGPR) has gained immense importance in the last decade due to its in-depth study and role of rhizosphere as an ecological unit biosphere. A putative PGPR is considered only when it may have a positive impact on plant after inoculation. From various pieces literature, been found that these bacteria improve growth plants their products through growth-promoting activities. microbial consortium effect (PGP) activities evident by literature. In natural ecosystem, rhizobacteria interact synergistically antagonistically with each other form consortium, but there are oscillating environmental conditions affect potential mechanism consortium. For sustainable development our environment, utmost necessity maintain stability rhizobacterial fluctuating conditions. decade, studies conducted design synthetic helps integrate cross-feeding over strains reveal social interactions. this review, authors emphasized covering all designing consortiums, strategies, mechanism, application field ecology biotechnology.
Language: Английский
Citations
41
Nature Chemical Biology, Journal Year: 2023, Volume and Issue: 19(8), P. 951 - 961
Published: May 29, 2023
Abstract Nutritional codependence (syntrophy) has underexplored potential to improve biotechnological processes by using cooperating cell types. So far, design of yeast syntrophic communities required extensive genetic manipulation, as the co-inoculation most eukaryotic microbial auxotrophs does not result in cooperative growth. Here we employ high-throughput phenotypic screening systematically test pairwise combinations auxotrophic Saccharomyces cerevisiae deletion mutants. Although coculture pairs do enter growth, identify 49 that spontaneously form syntrophic, synergistic communities. We characterized stability and growth dynamics nine cocultures demonstrated a pair tryptophan grow exchanging pathway intermediate rather than end products. then introduced malonic semialdehyde biosynthesis split between different auxotrophs, which resulted increased production. Our results report spontaneous formation stable syntrophy S. illustrate dividing labor intraspecies community.
Language: Английский
Citations
33
Current Opinion in Biotechnology, Journal Year: 2023, Volume and Issue: 79, P. 102880 - 102880
Published: Jan. 6, 2023
Language: Английский
Citations
32
Trends in biotechnology, Journal Year: 2023, Volume and Issue: 41(11), P. 1430 - 1441
Published: June 16, 2023
Language: Английский
Citations
27
The Microbe, Journal Year: 2024, Volume and Issue: 4, P. 100117 - 100117
Published: July 4, 2024
Bacterial cells produce a variety of exopolysaccharide (EPS) throughout their life cycle. EPS plays crucial role in the formation biofilms and control environmental processes microbial communities. The bacterial community benefits from several ways, including nutrient compartmentation storage, stress resistance synergism. generation by consortium-associated bacteria, which involves microorganisms, is versatile efficient for use industrial processes, such as biotechnology pollution degradation. qualitative quantitative chemical characteristics consortium's are mostly determined type culture utilized, type, temperature, pH. main goal study was to find out how biosynthesis works consortium culture. To achieve maximum regeneration, pH, carbon, nitrogen sources were optimized. A three strains i.e. Klebsiella pneumonia strain, Pseudomonas aeruginosa strain Burkholderia cepacian that distinguished capacity developed part study. total production detected after 8 days incubation at pH 7.5 (15.5 g/L) temperature 35 °C (12.60 g/L). optimum identified 17.84 g/L 21.07 when ammonium sulfate glucose employed carbon supplements, respectively. protein concentration 3067 µg/mL, more than carbohydrate (263.10 µg/mL) DNA (7.60 content. protein/carbohydrate ratio present 11.65, much higher prior investigations. FTIR analysis confirmed functional groups carbohydrates, proteins, lipids DNA, viz., CO, COOH, NH, OH, CH OCH3. study's findings revealed enhancing molecules lowering biofilm-related infections improving efficacy antimicrobial treatments wastewater.
Language: Английский
Citations
16
Journal of Sustainable Agriculture and Environment, Journal Year: 2024, Volume and Issue: 3(3)
Published: Sept. 1, 2024
Abstract Sustainable increase in agriculture productivity is confronted by over‐reliance and over‐use of synthetic chemical fertilizers. With a market projection $5.02 billion 2030, biofertilizers are gaining momentum as supplement and, some cases, an alternative to Biofertilizers can improve the nutritional supply plant simultaneously soil health, reduce greenhouse emissions, hence directly contribute towards environmental sustainability. Plant growth‐promoting microbes (PGPMs) particularly receiving significant attention biofertilizers. They widely known for their ability growth via increasing nutrient availability use efficiency. However, except few successful commercialization PGPM‐based inoculants still limited, mainly due lack field efficacy consistency. Lack effective formulation technologies that keep microbial viable during storage, transport application considered one key factors drive inconsistent In this review, we identify current challenges associated with inoculants. We propose future paths, including advancement potentially efficient, eco‐friendly cost‐effective. argue enhance global adoption biofertilizers, new innovations based on transdisciplinary approaches indispensable. The emerging framework should encompass robust quality control system at all stages. Additionally, active partnership between academic industry stakeholders will pave way enhanced
Language: Английский
Citations
14