Antiproliferative and Anticancer Activity of Chrysin on Human A549 Lung Cancer Cell Lines; Possible Clinical Application DOI Creative Commons

A Asghari,

Akram Firouzi‐Amandi,

Mehdi Dadashpour

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 1(4)

Published: Nov. 23, 2024

: The current study aimed to assess the anticancer properties of Chrysin (Chr) as a potential therapeutic option for cancer. For this purpose, we analyzed both its toxic effects on cells and levels genes related apoptosis following treatment with Chr. effect Chr cell proliferation expression apoptosis-related in A549 lung line was evaluated using MTT assay qPCR, respectively. findings from cytotoxicity test revealed that IC50 cancer 20 μM. This result indicates exhibits significant cytotoxic at concentration, underscoring powerful agent. Furthermore, real-time PCR analysis showed changes gene after Specifically, found decrease human telomerase reverse transcriptase (hTERT) Bcl2, associated growth survival. Conversely, increased Bax, promotes apoptosis. These molecular suggest may induce by altering balance between anti-apoptotic pro-apoptotic factors. In conclusion, indicate effectively inhibits through regulation expression. property positions promising candidate further investigation development strategies, additional studies are necessary understand mechanisms action benefits.

Language: Английский

Advancing therapeutic efficacy: nanovesicular delivery systems for medicinal plant-based therapeutics DOI
Mohamed J. Saadh, Mohammed Ahmed Mustafa, Sanjay Kumar

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: 397(10), P. 7229 - 7254

Published: May 3, 2024

Language: Английский

Citations

4
Methodological Advances in Liposomal Encapsulation Efficiency Determination: Systematic Review and Analysis DOI
Jinping Wang, Zirui Huang, Cheng Zhang

et al.

Journal of drug targeting, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 20

Published: March 24, 2025

Liposomes represent a highly promising drug delivery platform for wide range of pharmaceutical compounds. The encapsulation efficiency (EE) stands as critical quality attribute liposomal formulations. Accurate determination EE requires quantification at least two parameters among the three distinct populations: total content, encapsulated fraction, and free concentration. However, due to complex physicochemical characteristics liposomes, particularly their structural flexibility, surface charge properties, organic phase composition, direct measurement fractions presents significant analytical challenges. ability precisely quantify both concentrations in formulations enables rapid reliable evaluation efficiency, which is essential guiding formulation optimization ensuring consistent product during scale-up manufacturing processes. This review provides comprehensive analysis various techniques determination, including (reverse) dialysis, ultrafiltration centrifugation, differential centrifugation (ultra/low-speed), size exclusion chromatography, with particular emphasis on methodological characteristics, applicable ranges, advantages, limitations. Furthermore, we propose appropriate detection strategies assessment based specific laboratory capabilities properties investigational

Language: Английский

Citations

0
Comprehensive insights into pancreatic cancer treatment approaches and cutting-edge nanocarrier solutions: from pathology to nanomedicine DOI
Rohit Sharma, Sourabh Kumar,

Kumari Komal

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Language: Английский

Citations

0
Efficient PEGylated magnetic nanoniosomes for co-delivery of artemisinin and metformin: a new frontier in chemotherapeutic efficacy and cancer therapy DOI Creative Commons

Rasoul Shahbazi,

Zohreh Mirjafary, Nosratollah Zarghami

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 9, 2024

Two strategies were employed to modify the performance of nano-niosome drug delivery system. Initially, surface nano-niosomes underwent modification through inclusion polyethylene glycol, thereby altering its properties. Additionally, core was equipped with Fe

Language: Английский

Citations

3
The Emerging Role of Cell Membrane-coated Nanomaterials in Cancer Therapy DOI
Sankha Bhattacharya, Paul Beninger

Current Pharmaceutical Design, Journal Year: 2024, Volume and Issue: 30(10), P. 727 - 741

Published: March 1, 2024

Abstract: This review investigates the revolutionary application of cell membrane-coated nanoparticles (CMNPs) as a promising avenue for cancer therapy within embryonic landscape nanotechnology. Nanoparticles, pivotal in treatment, are systematically examined their diverse physicochemical structures, categorized organic (lipid-based, protein-based, and polymer-assisted) inorganic (carbon-based metal) varieties. A significant focus is placed on CMNPs, which serve an innovative drug delivery vehicle, overcoming limitations associated with conventional nanoparticle therapies. manuscript accurately explores advantages challenges various membranes, including those derived from cells, red blood platelets, stem white cells. Importance roles enhancing precision, immune system circumvention, targeted recognition. Detailed insights into crafting CMNPs provided, elucidating membrane extraction fusion techniques, such sonication, extrusion, co-extrusion, microfluidic electroporation. Maintaining integrity during benefits coating techniques augmenting biocompatibility underscored. comprehensive resource consolidates latest advancements delivery, positioning itself at forefront nanotechnology biomedicine research. Encapsulating methodologies like electrospray, chemical conjugation, this showcases expanding toolbox available to researchers dynamic field. Focusing unique characteristics multifaceted applications biomedical research, particularly tumour therapy. It provides indepth analysis stability, evasion capabilities, increased payload capacity, retained biological functionality. The outlines current future prospects chemotherapy, photothermal photodynamic therapy, immunotherapy, gene therapeutic methods. concludes by highlighting transformative potential reshaping treatment.

Language: Английский

Citations

2
Niosomes loaded with gold nanoparticles for enhanced radiation therapy in lung cancer DOI
Ramezan Ali Taheri, Hamidreza Fathi, Ali Sharafi

et al.

Nanomedicine, Journal Year: 2024, Volume and Issue: 19(27), P. 2257 - 2270

Published: Sept. 26, 2024

The present investigation aimed to develop niosomes containing gold nanoparticles (Nio-AuNPs) and evaluate the combinational effect of Nio-AuNPs x-ray radiation therapy (XRT) on growth inhibition potential induction apoptosis in A549 cell line.

Language: Английский

Citations

2
The simultaneous use of nanovesicles and magnetic nanoparticles for cancer targeting and imaging DOI
Sara Salatin, Maryam Azarfarin, Afsaneh Farjami

et al.

Therapeutic Delivery, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 15

Published: Nov. 20, 2024

Cancer is increasingly being recognized as a global health issue with considerable unmet medical need. Despite the rapid progression of anticancer pharmaceuticals, there are still significant challenges for effective management cancer. In many circumstances, cancer cells difficult to detect and treat. Combination nanovesicles (NVs) magnetic nanoparticles (MNPs), referred (MNVs), now well potential theranostic option improving treatment outcomes reducing adverse effects. MNVs can be used monitoring long-term fate functional benefits therapy. Moreover, MNV-mediated hyperthermia mechanism has been explored technique triggering cell death, and/or controlled release laden cargo. this review, we focus on unique characteristics promising avenue targeted drug delivery, diagnosis, treatments or tumor. discuss critical considerations related issues raised in area, which will guide future research toward better anti-cancer therapeutics clinical applications.

Language: Английский

Citations

1
Synthesis and Evaluation of the Anticancer Effects of Iron Oxide-Chitosan Magnetic Nanoparticles Loaded Atorvastatin on the Invasion and Tumorigenicity of C6 Cell Line in Glioblastoma Animal Model DOI
Omid Alavi, Hamid Babavalian, Fatemeh Shakeri

et al.

Journal of Polymers and the Environment, Journal Year: 2024, Volume and Issue: 32(12), P. 6742 - 6759

Published: Aug. 28, 2024

Language: Английский

Citations

0
Artemisinin-loaded mesoporous silica nanoparticles/electrospun poly(lactic-co-glycolic acid) composite nanofibers for enhanced anticancer efficiency in breast cancer cells DOI Creative Commons

Mohammad Eslami Vaghar,

Mehdi Dadashpour,

Elahe Derakhshan

et al.

Cancer Nanotechnology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 20, 2024

Antihyperglycemic drug artemisinin (Art) has recently gained attention as a potential anticancer treatment. In this study, the poly(lactic-co-glycolic acid) (PLGA) polymer was used to create Art-containing nanofibers (NFs) using electrospinning technique. The morphological characteristics, rate of degradation, and release profile NFs were described. addition, we examined both cytotoxic effects internalization reactive oxygen species (ROS), well expression levels apoptotic genes following treatment SK-BR-3 breast cancer cells with Art Art-loaded PLGA nanofibers. bead-free, smooth surface, randomly aligned electrospun released medication quickly at first then steadily for more than 2 weeks. They also showed rather steady deterioration over course 24 days. After 48h, exposed ART-loaded shown substantial cytotoxicity compared free Art. Additionally, effectively increased intracellular ROS levels, inducing death in cells. Gene studies further demonstrated ability produced significantly modify Bax Bcl-2 activate caspases-3 P53 Overall, results effectiveness, indicating that it may be employed an implantable delivery system decrease recurrence surgical resection.

Language: Английский

Citations

0
Antiproliferative and Anticancer Activity of Chrysin on Human A549 Lung Cancer Cell Lines; Possible Clinical Application DOI Creative Commons

A Asghari,

Akram Firouzi‐Amandi,

Mehdi Dadashpour

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 1(4)

Published: Nov. 23, 2024

: The current study aimed to assess the anticancer properties of Chrysin (Chr) as a potential therapeutic option for cancer. For this purpose, we analyzed both its toxic effects on cells and levels genes related apoptosis following treatment with Chr. effect Chr cell proliferation expression apoptosis-related in A549 lung line was evaluated using MTT assay qPCR, respectively. findings from cytotoxicity test revealed that IC50 cancer 20 μM. This result indicates exhibits significant cytotoxic at concentration, underscoring powerful agent. Furthermore, real-time PCR analysis showed changes gene after Specifically, found decrease human telomerase reverse transcriptase (hTERT) Bcl2, associated growth survival. Conversely, increased Bax, promotes apoptosis. These molecular suggest may induce by altering balance between anti-apoptotic pro-apoptotic factors. In conclusion, indicate effectively inhibits through regulation expression. property positions promising candidate further investigation development strategies, additional studies are necessary understand mechanisms action benefits.

Language: Английский

Citations

0