Multi-Omic Approaches in Cancer-Related Micropeptide Identification DOI Creative Commons
Katarina Vrbnjak, Raj Nayan Sewduth

Proteomes, Journal Year: 2024, Volume and Issue: 12(3), P. 26 - 26

Published: Sept. 13, 2024

Despite the advances in modern cancer therapy, malignant diseases are still a leading cause of morbidity and mortality worldwide. Conventional treatment methods frequently lead to side effects drug resistance patients, highlighting need for novel therapeutic approaches. Recent findings have identified existence non-canonical micropeptides, an additional layer proteome complexity, also called microproteome. These small peptides promising class agents with potential address limitations current treatments. The microproteome is encoded by regions genome historically annotated as non-coding, its has been revealed thanks recent proteomic bioinformatic technology, which dramatically improved understanding complexity. Micropeptides shown be biologically active several types, indicating their role. Furthermore, they characterized low toxicity high target specificity, demonstrating development better tolerated drugs. In this review, we survey landscape known micropeptides role progression or treatment, discuss anticancer agents, describe methodological challenges facing field research.

Language: Английский

Non-coding RNAs: emerging biomarkers and therapeutic targets in cancer and inflammatory diseases DOI Creative Commons
Basma Hossam Abdelmonem,

Lereen T. Kamal,

Lilian Waheed Wardy

et al.

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: March 10, 2025

Non-coding RNAs (ncRNAs) have a significant role in gene regulation, especially cancer and inflammatory diseases. ncRNAs, such as microRNA, long non-coding RNAs, circular alter the transcriptional, post-transcriptional, epigenetic expression levels. These molecules act biomarkers possible therapeutic targets because aberrant ncRNA has been directly connected to tumor progression, metastasis, response therapy research. ncRNAs’ interactions with multiple cellular pathways, including MAPK, Wnt, PI3K/AKT/mTOR, impact processes like proliferation, apoptosis, immune responses. The potential of RNA-based therapeutics, anti-microRNA microRNA mimics, restore normal is being actively studied. Additionally, tissue-specific patterns ncRNAs offer unique opportunities for targeted therapy. Specificity, stability, responses are obstacles use ncRNAs; however, novel strategies, modified oligonucleotides delivery systems, developed. profiling may result more individualized successful treatments precision medicine advances, improving patient outcomes creating early diagnosis monitoring opportunities. current review aims investigate roles diseases, focusing on their mechanisms regulation implications non-invasive diagnostics therapies. A comprehensive literature was conducted using PubMed Google Scholar, research published between 2014 2025. Studies were selected based rigorous inclusion criteria, peer-reviewed status relevance Non-English, non-peer-reviewed, inconclusive studies excluded. This approach ensures that findings presented high-quality relevant sources.

Language: Английский

Citations

1
Emerging Role of Endogenous Peptides Encoded by Non-coding RNAs in Cancer Biology DOI Creative Commons
Anna Lucia Tornesello, Andrea Cerasuolo, Noemy Starita

et al.

Non-coding RNA Research, Journal Year: 2024, Volume and Issue: 10, P. 231 - 241

Published: Oct. 30, 2024

Language: Английский

Citations

4
Peptide-driven strategies against lung cancer DOI

Vijayshree S Karankar,

Saurabh Awasthi, Nidhi Srivastava

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 123453 - 123453

Published: Feb. 1, 2025

Language: Английский

Citations

0
Noncoding RNA-encoded peptides in cancer: biological functions, posttranslational modifications and therapeutic potential DOI Creative Commons

Shiming Tan,

Wenjuan Yang, Zongyao Ren

et al.

Journal of Hematology & Oncology, Journal Year: 2025, Volume and Issue: 18(1)

Published: Feb. 19, 2025

In the present era, noncoding RNAs (ncRNAs) have become a subject of considerable scientific interest, with peptides encoded by ncRNAs representing particularly promising avenue investigation. The identification ncRNA-encoded in human cancers is increasing. These regulate cancer progression through multiple molecular mechanisms. Here, we delineate patterns diverse and provide synopsis methodologies employed for that possess capacity to encode these peptides. Furthermore, discuss impacts on biological behavior cells underlying conclusion, describe prospects challenges need be overcome.

Language: Английский

Citations

0
Endogenous micropeptides as potential diagnostic biomarkers and therapeutic drugs DOI Creative Commons
Shiqi Zhong, Shuai Li, Jingxuan Zhang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 8, 2025

Micropeptides, these small proteins derived from non-coding RNA, typically consist of no more than 100 amino acids in length. Despite the challenges analysis and identification, their various critical functions within organisms cannot be overlooked. They play a significant role maintaining energy metabolism balance, regulating immune system, influencing development tumors, which also gives them decisive impact on occurrence diseases. This review aims to outline potential value micropeptides, introducing tissue classification distribution, biological functions, mechanisms, with focus as diagnostic markers therapeutic drugs.

Language: Английский

Citations

0
Emerging roles for microproteins as critical regulators of endoplasmic reticulum function and cellular homeostasis DOI Creative Commons
Taylor Coughlin, Catherine A. Makarewich

Seminars in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 170, P. 103608 - 103608

Published: April 17, 2025

Language: Английский

Citations

0
Multidimensional insights into squalene epoxidase drug development: in vitro mechanisms, in silico modeling, and in vivo implications DOI
Ahmed A. Allam, Hassan A. Rudayni, Noha Ahmed

et al.

Expert Opinion on Therapeutic Targets, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Squalene epoxidase (SQLE) is a pivotal enzyme in sterol biosynthesis, catalyzing the conversion of squalene to 2,3-oxidosqualene. Beyond its core role cholesterol homeostasis, SQLE implicated cancer, hypercholesterolemia, and fungal infections, positioning it as valuable therapeutic target. We conducted comprehensive literature search across primary databases gather vitro, silico, vivo evidence on SQLE. This review explores enzyme's structural functional features, including substrate specificity catalytic mechanisms, examines inhibitor interactions. Computational methods predict - dynamics, guiding drug design, while investigations clarify SQLE's metabolic disorders tumorigenesis. Challenges include resistance study discrepancies, but emerging technologies, such cryo-electron microscopy CRISPR editing, offer new avenues for deeper exploration. an underexplored yet promising target, with particular relevance oxidative stress, ferroptosis, gut microbiota research. Overcoming current barriers through advanced technologies multidisciplinary strategies could propel SQLE-targeted treatments into clinical practice, supporting precision medicine broader translational applications.

Language: Английский

Citations

0
From non‐coding RNAs to cancer regulators: The fascinating world of micropeptides DOI
Can Li, Dan Zhang, Jinxi Huang

et al.

International Journal of Cancer, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Abstract Micropeptides are commonly identified as peptides encoded by non‐coding RNAs (ncRNAs). In the short open reading frame (sORF) of ncRNAs, there is a base sequence encoding functional micropeptides, which great significance in biological field. Recently, micropeptides regulate diverse processes, including mitochondrial metabolism, calcium transport, mRNA splicing, signal transduction, myocyte fusion, and cellular senescence, regulating homeostasis internal environment cancer's incidence progression. Especially, study cancer about potential regulatory mechanism will be conducive to further understanding process initiation development. More more research shows have been confirmed play an essential role emergence multiple kinds cancers, Breast cancer, Colon Colorectal Glioma, Glioblastoma, Liver cancer. This review presents comprehensive synthesis latest advancements our roles cells, with particular focus on networks involving oncogenesis. The new mode action provides innovative ideas for diagnosis treatment. Moreover, we explored significant capacity diagnostic biomarkers targets anti‐cancer therapies clinical settings, highlighting their development micropeptide‐based tools therapeutics.

Language: Английский

Citations

0
Exploring the Dark Matter of Human Proteome: Emerging Role of Non-canonical Open Reading Frame (ncORF) in Cancer Diagnosis, Biology, and Therapy DOI Open Access
Anni Ge,

Curtis Chan,

Xiaolong Yang

et al.

Published: June 28, 2024

Cancer develops from abnormal cell growth in the body, causing significant mortalities every year. To date, potent therapeutic approaches have been developed to eradicate tumor cells, but intolerable toxicity and drug resistance can occur treated patients, limiting efficiency of existing treatment strategies. Therefore, searching for novel genes critical cancer progression response is urgently needed successful therapy. Recent advances bioinformatic proteomic techniques allowed identification a category peptides encoded by non-canonical open reading frames (ncORFs) historically non-coding genomic regions. Surprisingly, many ncORFs express functional microproteins that play vital role human cancers. In this review, we provide comprehensive description different ncORF types with coding capacity technological methods discovering among genomes. We also summarize diagnostic prognostic value these as well their carcinogenic underestimated potential targets.

Language: Английский

Citations

1
Exploring the Dark Matter of Human Proteome: The Emerging Role of Non-Canonical Open Reading Frame (ncORF) in Cancer Diagnosis, Biology, and Therapy DOI Open Access
Anni Ge,

Curtis Chan,

Xiaolong Yang

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(15), P. 2660 - 2660

Published: July 26, 2024

Cancer develops from abnormal cell growth in the body, causing significant mortalities every year. To date, potent therapeutic approaches have been developed to eradicate tumor cells, but intolerable toxicity and drug resistance can occur treated patients, limiting efficiency of existing treatment strategies. Therefore, searching for novel genes critical cancer progression response is urgently needed successful therapy. Recent advances bioinformatics proteomic techniques allowed identification a category peptides encoded by non-canonical open reading frames (ncORFs) historically non-coding genomic regions. Surprisingly, many ncORFs express functional microproteins that play vital role human cancers. In this review, we provide comprehensive description different ncORF types with coding capacity technological methods discovering among genomes. We also summarize carcinogenic such as pTINCR HOXB-AS3 regulating hallmarks cancer, well roles CIP2A-BP diagnosis prognosis. discuss how AKT-174aa DDUP are involved anti-cancer underestimated potential targets.

Language: Английский

Citations

1