Intracellular and intercellular transport of RNA organelles in CXG repeat disorders: The strength of weak ties DOI Creative Commons
Deepti Kailash Nabariya, Annika Heinz,

Sabrina Derksen

et al.

Frontiers in Molecular Biosciences, Journal Year: 2022, Volume and Issue: 9

Published: Dec. 16, 2022

RNA is a vital biomolecule, the function of which tightly spatiotemporally regulated. organelles are biological structures that either membrane-less or surrounded by membrane. They produced all cells and indulge in cellular mechanisms. include intracellular granules extracellular exosomes. play an essential role regulation localization, stability translation. Aberrant connected to disease development. For example, microsatellite diseases such as CXG repeat expansion disorders, mutant RNA’s localization affected. not only transported intracellularly but can also be between via The loading exosomes regulated RNA-protein complexes, recent studies show cytosolic share common content. Intracellular exosome may therefore related. Exosomes transfer pathogenic molecules from cell cell, thereby driving progression. Both vesicles serve source for diagnostic treatment strategies. In therapeutic approaches, pharmaceutical agents loaded into then transport them desired cells/tissues. This promising target specific strategy with few side effects. With respect diagnostics, disease-specific content exosomes, e.g., RNA-signatures, attractive biomarker central nervous system detecting early physiological disturbances, even before symptoms neurodegeneration appear irreparable damage occurs. this review, we summarize known cytoplasmic vesicles, well their dysfunction disorders. We provide summary established protocols isolation characterization both organelles.

Language: Английский

Extracellular Vesicles as Therapeutic Resources in the Clinical Environment DOI Open Access
Jorge Sanz‐Ros, Cristina Mas‐Bargues, Nekane Romero-García

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 2344 - 2344

Published: Jan. 25, 2023

The native role of extracellular vesicles (EVs) in mediating the transfer biomolecules between cells has raised possibility to use them as therapeutic vehicles. development therapies based on EVs is now expanding rapidly; here we will describe current knowledge different key points regarding a clinical setting. These are related cell sources EVs, isolation, storage, and delivery methods, well modifications releasing for improved production EVs. Finally, depict application trials, considering impact COVID-19 pandemic these therapies, pointing out that although it promising therapy human diseases, still initial phase its patients.

Language: Английский

Citations

53
Milk exosomes: an oral drug delivery system with great application potential DOI

Meng‐Yuan Tian,

Dong-Xia Hao,

Yang Liu

et al.

Food & Function, Journal Year: 2023, Volume and Issue: 14(3), P. 1320 - 1337

Published: Jan. 1, 2023

Exosomes are extracellular vesicles with the smallest diameter, usually divided into cellular sources and body fluid sources.

Language: Английский

Citations

33
Targeting delivery of miR-146a via IMTP modified milk exosomes exerted cardioprotective effects by inhibiting NF-κB signaling pathway after myocardial ischemia-reperfusion injury DOI Creative Commons

Wan-ting Meng,

Jing Zhu,

Ya-chao Wang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: July 1, 2024

Abstract Reperfusion therapy is critical for saving heart muscle after myocardial infarction, but the process of restoring blood flow can itself exacerbate injury to myocardium. This phenomenon known as ischemia-reperfusion (MIRI), which includes oxidative stress, inflammation, and further cell death. microRNA-146a (miR-146a) play a significant role in regulating immune response has been studied its potential impact on improvement function injury. However, delivery miR-146a specific efficient manner remains challenge extracellular RNAs are unstable rapidly degraded. Milk exosomes (MEs) have proposed ideal platform miRNA-based they protect miRNAs from RNase degradation. In this study, effects containing MEs (MEs-miR-146a) cardiac were examined rat model MIRI. To enhance targeting MEs-miR-146a site injury, ischemic myocardium-targeted peptide IMTP was modified onto surfaces, whether could exert better therapeutic by echocardiography, indicators levels inflammatory factors. Furthermore, expressions mediated NF-κB signaling pathway-related proteins detected western blotting qRT-PCR elucidate mechanisms. MiR-146 mimics successfully loaded into electroporation at square wave 1000 V voltage 0.1 ms pulse duration. be up-taken cardiomyocytes protected cells oxygen glucose deprivation/reperfusion induced damage vitro. Oral administration decreased tissue apoptosis expression factors improved The level myocardium tissues significantly increased MEs-miR-146a, higher than that group. addition, intravenous injection enhanced heart, function, reduced suppressed inflammation MIRI, more effective treatment. Moreover, protein IRAK1, TRAF6 p-p65. Therefore, exerted their anti-inflammatory effect inhibiting IRAK1/TRAF6/NF-κB pathway. Taken together, our findings suggested may promising strategy treatment MIRI with outcome modification peptide, expected applied clinical practice future.

Language: Английский

Citations

14
A Comprehensive Review of the Development and Therapeutic Use of Antivirals in Flavivirus Infection DOI Creative Commons
Aarti Tripathi,

Shailendra Chauhan,

Renu Khasa

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(1), P. 74 - 74

Published: Jan. 8, 2025

Flaviviruses are a diverse group of viruses primarily transmitted through hematophagous insects like mosquitoes and ticks. Significant expansion in the geographic range, prevalence, vectors flavivirus over last 50 years has led to dramatic increase infections that can manifest as hemorrhagic fever or encephalitis, leading prolonged morbidity mortality. Millions every year pose serious threat worldwide public health, encouraging scientists develop better understanding pathophysiology immune evasion mechanisms these for vaccine development antiviral therapy. Extensive research been conducted developing effective antivirals flavivirus. Various approaches have extensively utilized clinical trials development, targeting virus entry, replication, polyprotein synthesis processing, egress pathways exploiting well host proteins. However, date, no licensed drug exists treat diseases caused by viruses. Understanding host-pathogen interaction, immunity, viral evasion, disease pathogenesis is highly warranted foster antivirals. This review provides an detailed summary most recent advances drugs combat diseases.

Language: Английский

Citations

1
Immunotherapy in mastitis: state of knowledge, research gaps and way forward DOI Creative Commons
Afnan Saleem, Sahar Saleem,

Faith Omonijo

et al.

Veterinary Quarterly, Journal Year: 2024, Volume and Issue: 44(1), P. 1 - 23

Published: July 7, 2024

Mastitis is an inflammatory condition that affects dairy cow's mammary glands. Traditional treatment approaches with antibiotics are increasingly leading to challenging scenarios such as antimicrobial resistance. In order mitigate the unwanted side effects of antibiotics, alternative strategies those harness host immune system response, also known immunotherapy, have been implemented. Immunotherapy treat bovine mastitis aims enhance response against pathogens by promoting pathogen clearance, and facilitating tissue repair. Various studies demonstrated potential immunotherapy for reducing incidence, duration severity mastitis. Nevertheless, majority reported therapies lacking in specificity hampering their broad application Meanwhile, advancements hold great promise industry, provide effective sustainable alternatives traditional antibiotic-based approaches. This review synthesizes strategies, current understanding future perspectives. The perspectives should focus on development precision immunotherapies tailored address individual pathogens/group pathogens, combination resistance, integration nano- omics technologies. By addressing research gaps, field can make significant strides control, prevention mastitis, ultimately benefiting both animal human health/welfare, environment health.

Language: Английский

Citations

6
Nanomaterial Assisted Exosome Analysis Using Mass Spectrometry DOI
Yi Zhang,

Bokai Zhou,

Qiu-Ning Li

et al.

Chemical Research in Chinese Universities, Journal Year: 2024, Volume and Issue: 40(2), P. 237 - 254

Published: March 20, 2024

Language: Английский

Citations

5
Milk-Derived Extracellular Vesicles: Biomedical Applications, Current Challenges, and Future Perspectives DOI
Mahsa Salehi, Babak Negahdari, Fatemeh Mehryab

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(15), P. 8304 - 8331

Published: April 8, 2024

Extracellular vesicles (EVs) are nano to-micrometer-sized sacs that released by almost all animal and plant cells act as intercellular communicators transferring their cargos between the source target cells. As a safe scalable alternative to conditioned medium-derived EVs, milk-derived EVs (miEVs) have recently gained great deal of popularity. Numerous studies shown miEVs intrinsic therapeutic actions can treat diseases enhance human health. Additionally, they be used natural drug carriers novel classes biomarkers. However, due complexity milk, successful translation from benchtop bedside still faces several unfilled gaps, especially lack standardized protocols for isolation high-purity miEVs. In this work, comprehensively reviewing bovine studies, we provide an overview current knowledge research on while highlighting challenges enormous promise class theranostics. It is hoped study will pave way clinical applications addressing opportunities.

Language: Английский

Citations

5
Utilization of Local Remedies among Dengue Patients Admitted to the Emergency Department of a Tertiary Care Center: An Observational Study DOI Open Access
Avichal Rajpal, Ashok Kumar Pannu, Ashish Behera

et al.

Indian Journal of Critical Care Medicine, Journal Year: 2025, Volume and Issue: 29(2), P. 148 - 150

Published: Jan. 30, 2025

Dengue viral infection (DVI) affects ~ 400 million people annually, with 100 cases causing clinical illness. Limited therapeutic options often lead patients to adopt alternative remedies. This study evaluates the prevalence and impact of such remedies on outcomes in admitted a tertiary care emergency department. A single-center, observational was conducted from July 2022 September 2023, including 170 aged >12 years severe DVI or warning signs, as per World Health Organization (WHO) criteria. The use local goat's milk, papaya leaves/extract, neem leaves, giloy juice, medicine documented. Outcomes, mortality hospital stay duration, were compared between remedy users non-users. mean age participants 36 years, 60% males 50% rural areas. 35.29% used remedies, milk (23.52%) leaves/extract (15.29%) being most common. median duration 2 days. Mortality 6.67% 8.4% non-users (p = 0.28). 4 days for both groups, no significant outcome differences. One-third prevalent. However, observed. Rajpal A, Pannu AK, Behera Sharma N, Hanumanthappa MK. Utilization Local Remedies among Patients Admitted Emergency Department Tertiary Care Center: An Observational Study. Indian J Crit Med 2025;29(2):148-150.

Language: Английский

Citations

0
A One-Pot Rpa-Crispr/Cas12a Rapid Diagnostic Platform for Dengue Virus DOI
Yuhong Guan, Xinyu Zhang, Min Yu

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Persistence of Tick-Borne Encephalitis Virus in Goat and Cow Milks Under Different Storage Conditions and Following Thermal Inactivation DOI Creative Commons

Laure Mathews-Martin,

Camille Victoire Migné,

Teheipuaura Mariteragi-Helle

et al.

Food and Environmental Virology, Journal Year: 2025, Volume and Issue: 17(2)

Published: April 18, 2025

Language: Английский

Citations

0