Co-culture engineering: a promising strategy for production of engineered extracellular vesicle for osteoarthritis treatment DOI Creative Commons

Abazar Esmaeili,

Samaneh Hosseini, Mohamadreza Baghaban Eslaminejad

et al.

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: Jan. 10, 2024

Abstract The therapeutic effects of extracellular vesicles (EVs) have been identified as a significant factor in intercellular communication different disease treatments, including osteoarthritis (OA). Compared to the conventional approaches treating OA, EV therapy is non-invasive and cell-free method. However, improving yield EVs their are main challenges for clinical applications. In this regard, researchers using engineering potential overcome these challenges. New findings suggest that co-culture strategy an indirect method efficiently increases production quality. mesenchymal stem cells (MSCs) chondrocytes has improved chondrogenesis, anti-inflammatory effects, regenerative properties which mediated by EVs. Hence, considerable systems could be useful producing engineered Here, we review various approaches, diverse direct indirect, 2D 3D cell cultures, well static dynamic systems. Meanwhile, discuss advantages combined strategies achieve OA treatment.

Language: Английский

Migrasomes, a new mode of intercellular communication DOI Creative Commons

Yuyun Jiang,

Xi Liu,

Jixian Ye

et al.

Cell Communication and Signaling, Journal Year: 2023, Volume and Issue: 21(1)

Published: May 8, 2023

Abstract Migrasomes are newly discovered extracellular vesicles (EVs) that formed in migrating cells and mediate intercellular communication. However, their size, biological generation, cargo packaging, transport, effects on recipient by migrasomes different from those of other EVs. In addition to mediating organ morphogenesis during zebrafish gastrulation, discarding damaged mitochondria, lateral transport mRNA proteins, growing evidence has demonstrated a variety pathological processes. this review, we summarize the discovery, mechanisms formation, isolation, identification, mediation cellular communication migrasomes. We discuss migrasome-mediated disease processes, such as osteoclast differentiation, proliferative vitreoretinopathy, tumor cell metastasis PD-L1 immune chemotaxis site infection chemokines, angiogenesis promotion via angiogenic factors cells, leukemic mesenchymal stromal cells. Moreover, new EVs, propose potential for diagnosis treatment.

Language: Английский

Citations

48
Therapeutic potential of RNA-enriched extracellular vesicles: The next generation in RNA delivery via biogenic nanoparticles DOI

Muskan Muskan,

Pevindu Abeysinghe,

Riccardo Cecchin

et al.

Molecular Therapy, Journal Year: 2024, Volume and Issue: 32(9), P. 2939 - 2949

Published: Feb. 27, 2024

Language: Английский

Citations

38
A critical systematic review of extracellular vesicle clinical trials DOI Creative Commons
Rachel R. Mizenko,

Madison Feaver,

Batuhan Turhan Bozkurt

et al.

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(10)

Published: Sept. 27, 2024

Abstract This systematic review examines the landscape of extracellular vesicle (EV)‐related clinical trials to elucidate field's trends in applications and EV‐related methodologies, with an additional focus on acknowledgement EV subpopulations. By analysing data from public reporting repositories, we catalogued 471 date, indications for over 200 diseases. Diagnostics companion diagnostics represented bulk cancer being most frequent application. therapeutics mainly utilized mesenchymal stromal cell (MSC) EVs were frequently used treatment respiratory illnesses. Ultracentrifugation RNA‐sequencing common isolation characterization techniques; however, methodology each was not reported study records. Most relied isolates, only 11% utilizing subpopulations their experimental design. While this may be connected a lack available techniques suitable implementation, it also highlights opportunity use improve translational efforts. As academic research identifies more chemically distinct technologies enrichment, forecast refined near future. emphasizes need meticulous methodological consideration enhance success EV‐based interventions, pointing towards paradigm shift personalized medicine.

Language: Английский

Citations

20
Emerging Strategies for Revascularization: Use of Cell-Derived Extracellular Vesicles and Artificial Nanovesicles in Critical Limb Ischemia DOI Creative Commons

Vijay Murali Ravi Mythili,

Ramya Lakshmi Rajendran, Arun Renganathan

et al.

Bioengineering, Journal Year: 2025, Volume and Issue: 12(1), P. 92 - 92

Published: Jan. 20, 2025

Critical limb ischemia (CLI) poses a substantial and intricate challenge in vascular medicine, necessitating the development of innovative therapeutic strategies to address its multifaceted pathophysiology. Conventional revascularization approaches often fail adequately complexity CLI, identification alternative methodologies. This review explores uncharted territory beyond traditional therapies, focusing on potential two distinct yet interrelated entities: cell-derived extracellular vesicles (EVs) artificial nanovesicles. Cell-derived EVs are small membranous structures naturally released by cells, nanovesicles artificially engineered nanosized vesicles. Both these represent promising avenues for intervention. They act as carriers bioactive cargo, including proteins, nucleic acids, lipids, that can modulate cellular responses associated with ischemic tissue repair angiogenesis. also assesses evolving landscape CLI through unique perspective The spans spectrum from early preclinical investigations latest translational advancements, providing comprehensive overview current state research this emerging field. These groundbreaking vesicle therapies hold immense revolutionizing treatment paradigms.

Language: Английский

Citations

2
Stem cell therapy for cardiac regeneration: past, present, and future DOI Creative Commons

Jaideep Kaur Gill,

Sargun Kaur Rehsia,

Elika Verma

et al.

Canadian Journal of Physiology and Pharmacology, Journal Year: 2024, Volume and Issue: 102(3), P. 161 - 179

Published: Jan. 16, 2024

Cardiac disorders remain the leading cause of mortality worldwide. Current clinical strategies, including drug therapy, surgical interventions, and organ transplantation offer limited benefits to patients without regenerating damaged myocardium. Over past decade, stem cell therapy has generated a keen interest owing its unique self-renewal immune privileged characteristics. Furthermore, ability cells differentiate into specialized types, made them popular therapeutic tool against various diseases. This comprehensive review provides an overview potential different types in reference cardiovascular it sheds light on advantages limitations associated with each type. An in-depth analysis challenges research hurdles for translation their possible solutions have also been elaborated upon. It examines controversies surrounding embryonic emergence alternative approaches, such as use induced pluripotent cardiac applications. Overall, this serves valuable resource researchers, clinicians, policymakers involved field regenerative medicine, guiding development safe effective cell-based therapies revolutionize patient care.

Language: Английский

Citations

10
Optimizing mesenchymal stem cell extracellular vesicles for chronic wound healing: Bioengineering, standardization, and safety DOI Creative Commons
Yusuke Shimizu, Edward Hosea Ntege, Yoshikazu Inoue

et al.

Regenerative Therapy, Journal Year: 2024, Volume and Issue: 26, P. 260 - 274

Published: June 1, 2024

Chronic wounds represent a significant global burden, afflicting millions with debilitating complications. Despite standard care, impaired healing persists due to factors like persistent inflammation and tissue regeneration. Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) offer an innovative regenerative medicine approach, delivering cell-derived therapeutic cargo in engineered nanoscale delivery systems. This review examines pioneering bioengineering strategies engineer MSC-EVs into precision nanotherapeutics for chronic wounds. Emerging technologies CRISPR gene editing, microfluidic manufacturing, biomimetic systems are highlighted their potential enhance MSC-EV targeting, optimize enrichment, ensure consistent clinical-grade production. However, key hurdles remain, including batch variability, rigorous safety assessment tumorigenicity, immunogenicity, biodistribution profiling. Crucially, collaborative frameworks harmonizing regulatory science patient advocacy hold the expediting clinical translation. By overcoming these challenges, could catalyze new era of off-the-shelf therapies, restoring hope afflicted by non-healing

Language: Английский

Citations

9
Stabilizing milk-derived extracellular vesicles (mEVs) through lyophilization: a novel trehalose and tryptophan formulation for maintaining structure and Bioactivity during long-term storage DOI Creative Commons
Alan B. Dogan, Spencer R. Marsh,

Rachel J. Tschetter

et al.

Journal of Biological Engineering, Journal Year: 2025, Volume and Issue: 19(1)

Published: Jan. 13, 2025

Abstract Extracellular vesicles (EVs) are widely investigated for their implications in cell-cell signaling, immune modulation, disease pathogenesis, cancer, regenerative medicine, and as a potential drug delivery vector. However, maintaining integrity bioactivity of EVs between Good Manufacturing Practice separation/filtration end-user application remains consistent bottleneck towards commercialization. Milk-derived extracellular (mEVs), separated from bovine milk, could provide relatively low-cost, scalable platform large-scale mEV production; however, the reliance on cold supply chain storage logistical financial burden biologics that unstable at room temperature. Herein, we aim to characterize engineer freeze-dried, formulation can be stored temperature without sacrificing structure/bioactivity reconstituted before delivery. In addition undertaking established assays structure function our preparations, introduce novel, efficient, high throughput assay based Electric Cell Substrate Impedance Sensing (ECIS) Human dermal fibroblast monolayers. By adding appropriate excipients, such trehalose tryptophan, describe protective preserves during long-term, storage. Our identification efficacy tryptophan novel additive lyophilization solutions represent significant advancement stabilizing small outside conditions.

Language: Английский

Citations

1
Extracellular Vesicles as Mediators of Endothelial Dysfunction in Cardiovascular Diseases DOI Open Access
Francisco Rafael Jimenez‐Trinidad,

Sergi Calvo-Gomez,

Manel Sabaté

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1008 - 1008

Published: Jan. 24, 2025

This comprehensive review aims to provide a thorough overview of the vital role that extracellular vesicles (EVs) play in endothelial dysfunction, particularly emphasizing how physiological factors—such as sex and aging—along with significant cardiovascular risk factors, influence this process. The covers studies ranging from first description EVs 1945 contemporary insights into their biological roles intercellular signaling dysfunction. A analysis peer-reviewed articles reviews indexed PubMed database was conducted compile information. Initially, Medical Subject Headings (MeSH) terms included keywords aimed at providing general knowledge about regulation signaling, such “extracellular vesicles”, “endothelium”, “intercellular signaling”. Subsequently, related pathophysiological implications EV interactions dysfunction disease were added, including “cardiovascular disease”, “sex”, “aging”, “atherosclerosis”, “obesity”, “diabetes”. Additionally, potential applications explored using MeSH “biomarker”, “therapeutic strategy”. results bibliographical reveal have capacity induce various cellular responses within system complex landscape disease. composition cargo is subject modification by conditions sex, aging, which result regulatory on function neighboring cells when released dysfunctional endothelium. Moreover, data suggest field still requires further exploration, biology continuously evolving, presenting dynamic engaging area for research. deeper understanding molecular involved EV–endothelium could yield valuable biomarkers monitoring progression facilitate development innovative bioengineered therapeutic strategies enhance patient outcomes.

Language: Английский

Citations

1
The Potential Use of Mitochondrial Extracellular Vesicles as Biomarkers or Therapeutical Tools DOI Open Access
Jorge Sanz‐Ros, Cristina Mas‐Bargues, Nekane Romero-García

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(8), P. 7005 - 7005

Published: April 10, 2023

The mitochondria play a crucial role in cellular metabolism, reactive oxygen species (ROS) production, and apoptosis. Aberrant can cause severe damage to the cells, which have established tight quality control for mitochondria. This process avoids accumulation of damaged lead release mitochondrial constituents extracellular milieu through vesicles (MitoEVs). These MitoEVs carry mtDNA, rRNA, tRNA, protein complexes respiratory chain, largest even transport whole Macrophages ultimately engulf these undergo outsourced mitophagy. Recently, it has been reported that also contain healthy mitochondria, whose function seems be rescue stressed cells by restoring loss function. transfer opened field their use as potential disease biomarkers therapeutic tools. review describes this new EVs-mediated current application clinical environment.

Language: Английский

Citations

18
Unraveling the surface marker signature of cell-derived vesicles via proteome analysis and nanoparticle flow cytometry DOI Creative Commons

Hui-Chong Lau,

Ilaria Passalacqua,

Jik‐Han Jung

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 2, 2024

Abstract The cell-derived vesicles (CDVs) obtained using a proprietary extrusion process are the foundation of BioDrone platform technology. With superior productivity and versatility, this technology has garnered increasing attention in broad applications, particularly as drug delivery vehicle. Previously, we showed that CDVs exhibited varying levels expression for tetraspanin organelle membrane markers while revealing no discernible differences physical characteristics compared to naturally produced extracellular (EVs). To further understand utilize therapeutic potentials CDVs, more comprehensive study protein profiles is necessary. In addition, it crucial validate from indeed intact lipid rather than other impurities. Here, multiple batches EVs HEK293 cells. were subjected same purification processes subsequent proteome particle analyses. analyses revealed unique signatures between EVs, parental Extensive identified nine most prominent abundant cells EVs. Subsequent western blotting nanoparticle flow cytometry confirmed CD63, lysosome-associated glycoprotein 1 (LAMP1), nicastrin (NCSTN) highly enriched whereas CD81, CD9, prostaglandin F2 receptor negative regulator (PTGFRN) This highlights composition marker signature distinct Lastly, demonstrated 90% genuine by combining two different classes vesicle labeling dyes detergents disrupt membranes. indicates our compatible with well-characterized EV production methods. robust CDV will also facilitate engineering achieve enhanced effects or tissue-selective cargo delivery.

Language: Английский

Citations

7