Pyroptosis in Spinal Cord Injury: Implications for Pathogenesis and Therapeutic Approaches DOI Creative Commons
Guangjin Gu,

Huaqi Yu,

Huishuang Zou

et al.

Frontiers in Bioscience-Landmark, Journal Year: 2024, Volume and Issue: 29(6), P. 210 - 210

Published: June 11, 2024

Traumatic spinal cord injury (SCI) is a serious disease of the central nervous system. Aside from limited intrinsic regenerative capacity neurons, complex microenvironmental disturbances can also lead to further cellular damage and growth inhibition. Programmed cell death regulated by pyroptosis has an important role in pathogenesis SCI. While there been wealth new knowledge regarding pyroptosis, detailed understanding its SCI possible therapeutic strategies still lacking. This review summarizes current advances regulatory pyroptosis-regulated inflammasome components inhibitory microenvironment following SCI, as well recent advances.

Language: Английский

Exosome-mediated repair of spinal cord injury: a promising therapeutic strategy DOI Creative Commons
Tong Yu, Lili Yang, Ying Zhou

et al.

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 2, 2024

Abstract Spinal cord injury (SCI) is a catastrophic to the central nervous system (CNS) that can lead sensory and motor dysfunction, which seriously affects patients' quality of life imposes major economic burden on society. The pathological process SCI divided into primary secondary injury, cascade amplified responses triggered by injury. Due complexity mechanisms SCI, there no clear effective treatment strategy in clinical practice. Exosomes, are extracellular vesicles endoplasmic origin with diameter 30–150 nm, play critical role intercellular communication have become an ideal vehicle for drug delivery. A growing body evidence suggests exosomes great potential repairing SCI. In this review, we introduce exosome preparation, functions, administration routes. addition, summarize effect mechanism various repair review efficacy combination other strategies Finally, challenges prospects use described.

Language: Английский

Citations

16
miRNAs in neurodegenerative diseases: from target screening to precision therapy DOI
Dongyi Liao, Yujie Zhang, Shuangyang Li

et al.

Neurological Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Language: Английский

Citations

3
M2 Microglia-derived Exosomes Promote Spinal Cord Injury Recovery in Mice by Alleviating A1 Astrocyte Activation DOI
Jing Zhang, Die Hu, Liping Li

et al.

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: 61(9), P. 7009 - 7025

Published: Feb. 17, 2024

Language: Английский

Citations

15
BMSCs-derived exosomes inhibit macrophage/microglia pyroptosis by increasing autophagy through the miR-21a-5p/PELI1 axis in spinal cord injury DOI Creative Commons

Jun Gu,

Jingyi Wu, Chunming Wang

et al.

Aging, Journal Year: 2024, Volume and Issue: 16(6), P. 5184 - 5206

Published: March 11, 2024

Spinal cord injury (SCI) results in a diverse range of disabilities and lacks effective treatment options. In recent years, exosomes derived from bone mesenchymal stem cells (BMSCs) have emerged as promising cell-free therapeutic approach for treating ischemic brain other inflammatory conditions. Macrophage/microglial pyroptosis has been identified contributing factor to neuroinflammation following SCI. The potential BMSC-derived macrophage/microglia pyroptosis-induced neuroinflammation, however, be determined. Our findings demonstrate that BMSCs can enhance motor function recovery mitigate subsequent SCI by upregulating the expression autophagy-related proteins inhibiting activation NLRP3 inflammasomes macrophage/microglia. Moreover, miR-21a-5p is markedly increased BMSCs-derived exosomes, knocking down eliminates beneficial effects administration; upregulation enhances administration. Mechanistically, positively regulates autophagy reducing PELI1 expression, which turn inhibits their pyroptosis. This research provides novel evidence effectively suppress through miR-21a-5p/PELI1 axis-mediated pathway, ultimately facilitating functional restoration particular, our constructed overexpression greatly improved efficacy spinal injury. These establish foundation prospective utilization biological intervention

Language: Английский

Citations

15
T cell interactions with microglia in immune-inflammatory processes of ischemic stroke DOI Creative Commons
Yuxiao Zheng, Zilin Ren, Ying Liu

et al.

Neural Regeneration Research, Journal Year: 2024, Volume and Issue: 20(5), P. 1277 - 1292

Published: April 16, 2024

The primary mechanism of secondary injury after cerebral ischemia may be the brain inflammation that emerges an ischemic stroke, which promotes neuronal death and inhibits nerve tissue regeneration. As first immune cells to activated microglia play important immunomodulatory role in progression condition. After peripheral blood (mainly T cells) are recruited central nervous system by chemokines secreted brain, where they interact with microglia) trigger a neuroimmune response. This review summarizes interactions between immune-inflammatory processes stroke. We found that, during demonstrate more pronounced synergistic effect. Th1, Th17, M1 can co-secrete pro-inflammatory factors, such as interferon-γ, tumor necrosis factor-α, interleukin-1β, promote neuroinflammation exacerbate injury. Th2, Treg, M2 jointly secrete anti-inflammatory interleukin-4, interleukin-10, transforming growth factor-β, inhibit neuroinflammation, well factors brain-derived neurotrophic factor regeneration repair Immune influence direction subsequent turn determines prognosis stroke patients. Clinical trials have been conducted on ways modulate toward communication using immunosuppressant fingolimod or overdosing Treg neural reduce damage caused However, studies relatively infrequent, clinical experience is still insufficient. In summary, cell subsets act synergistically regulate inflammatory progression, mainly secreting factors. future, key research for treatment could rooted enhancement secretion promoting generation Th2 cells, along activation M2-type microglia. These approaches alleviate facilitate tissues.

Language: Английский

Citations

9
Exosomal lncRNA RMRP-shuttled by Olfactory Mucosa-Mesenchymal Stem Cells Suppresses Microglial Pyroptosis to Improve Spinal Cord Injury via EIF4A3/SIRT1 DOI
Chuang Wang, Jiangshan Zhang, Wei-Ming Chen

et al.

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

Language: Английский

Citations

1
The landscape of extracellular vesicles combined with intranasal delivery towards brain diseases DOI
Jiale Li, Hanwen Zhang, Yingyu Jiang

et al.

Nano Today, Journal Year: 2024, Volume and Issue: 55, P. 102169 - 102169

Published: Jan. 26, 2024

Language: Английский

Citations

7
IPSC-NSCs-derived exosomal let-7b-5p improves motor function after spinal cord Injury by modulating microglial/macrophage pyroptosis DOI Creative Commons
Jie Liu,

Guang Kong,

Chenlin Lu

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: July 9, 2024

Abstract Background Following spinal cord injury (SCI), the inflammatory storm initiated by microglia/macrophages poses a significant impediment to recovery process. Exosomes play crucial role in transport of miRNAs, facilitating essential cellular communication through transfer genetic material. However, miRNAs from iPSC-NSCs-Exos and their potential mechanisms leading repair after SCI remain unclear. This study aims explore microglia/macrophage pyroptosis reveal mechanisms. Methods were characterized identified using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), Western blot. A mouse model series vivo vitro experiments conducted investigate therapeutic effects iPSC-NSCs-Exos. Subsequently, miRNA microarray rescue performed confirm SCI. Mechanistic studies carried out blot, luciferase activity assays, RNA-ChIP. Results Our findings revealed that iPSC-NSCs-derived exosomes inhibited at 7 days post-SCI, maintaining myelin integrity promoting axonal growth, ultimately improving mice motor function. The showed let-7b-5p be highly enriched iPSC-NSCs-Exos, LRIG3 was as target gene let-7b-5p. Through experiments, we uncovered connection between iPSC-NSCs microglia/macrophages, revealing novel for treating Conclusion In conclusion, discovered can package deliver let-7b-5p, regulating expression ameliorate enhance function highlights combined therapy with functional limiting inflammation following

Language: Английский

Citations

7
Mesenchymal stem cell-derived exosomes as a new drug carrier for the treatment of spinal cord injury: A review DOI Creative Commons

Lin-Fei Cheng,

Chaoqun You, Peng Cheng

et al.

Chinese Journal of Traumatology, Journal Year: 2024, Volume and Issue: 27(3), P. 134 - 146

Published: March 26, 2024

Spinal cord injury (SCI) is a devastating traumatic disease seriously impairing the quality of life in patients. Expectations to allow hopeless central nervous system repair itself after are unfeasible. Developing new approaches regenerate still priority. Exosomes derived from mesenchymal stem cells (MSC-Exo) have been proven robustly quench inflammatory response or oxidative stress and curb neuronal apoptosis autophagy following SCI, which key processes rescue damaged spinal neurons restore their functions. Nonetheless, MSC-Exo SCI received scant attention. In this review, we reviewed our previous work other studies summarize roles its underlying mechanisms. Furthermore, also focus on application exosomes as drug carrier SCI. particular, it combs advantages for imaging advantages, types, loading methods, etc., provides latest progress treatment especially carrier.

Language: Английский

Citations

6
Regulatory T Cells and Their Derived Cell Pharmaceuticals as Emerging Therapeutics Against Autoimmune Diseases DOI
Yu Liu,

Yiqiu Fu,

Rourou Miao

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: July 6, 2024

Abstract Caused by the loss in tolerance against self‐antigens, autoimmune diseases are chronic disorders that impact millions of individuals annually with significant economic burden. They triggered a deficiency quantity or function regulatory T (Treg) cells, which essential for maintaining self‐tolerance and preventing excessive immune responses. Several clinical trials over past decade have demonstrated safety feasibility certain Treg cell‐based therapies diseases, inspiring optimism among patients. Studies indicated targeted cell pharmaceuticals significantly promising, offering superior targeting, improved biocompatibility, prolonged blood circulation. Thus, delivery systems also extensively studied. This review describes role cells system both homeostasis development autoimmunity, purification expansion methods, derived pharmaceutical therapies, therapeutic potential beneficial to accelerating industrialization translation formulations based on cells.

Language: Английский

Citations

5