Strategies of functionalized GelMA-based bioinks for bone regeneration: Recent advances and future perspectives

Bioactive Materials, Journal Year: 2024, Volume and Issue: 38, P. 346 - 373

Published: May 9, 2024

Gelatin methacryloyl (GelMA) hydrogels is a widely used bioink because of its good biological properties and tunable physicochemical properties, which has been in variety tissue engineering regeneration. However, pure GelMA limited by the weak mechanical strength lack continuous osteogenic induction environment, difficult to meet needs bone repair. Moreover, are unable respond complex stimuli therefore adapt physiological pathological microenvironments. This review focused on functionalization strategies hydrogel based bioinks for The synthesis process was described details, various functional methods requirements regeneration, including strength, porosity, vascularization, differentiation, immunoregulation patient specific repair, etc. In addition, response smart GelMA-based external physical stimulation internal microenvironment stimulation, as well achieve both disease treatment regeneration presence common diseases (such inflammation, infection, tumor) also briefly reviewed. Finally, we emphasized current challenges possible exploration directions

Language: Английский

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Three‐dimensional bioprinting biphasic multicellular living scaffold facilitates osteochondral defect regeneration

Interdisciplinary materials, Journal Year: 2024, Volume and Issue: 3(5), P. 738 - 756

Published: June 2, 2024

Abstract Due to tissue lineage variances and the anisotropic physiological characteristics, regenerating complex osteochondral tissues (cartilage subchondral bone) remains a great challenge, which is primarily due distinct requirements for cartilage bone regeneration. For regeneration, significant amount of newly generated chondrocytes required while maintaining their phenotype. Conversely, regeneration necessitates inducing stem cells differentiate into osteoblasts. Additionally, construction interface crucial. In this study, we fabricated biphasic multicellular bioprinted scaffold mimicking natural employing three‐dimensional (3D) bioprinting technology. Briefly, gelatin‐methacryloyl (GelMA) loaded with articular marrow mesenchymal (ACs/BMSCs), serving as layer, preserved phenotype ACs promoted differentiation BMSCs through interaction between BMSCs, thereby facilitating GelMA/strontium‐substituted xonotlite (Sr‐CSH) regulated osteoblasts enhanced secretion matrix by in layer slow release bioactive ions from Sr‐CSH. GelMA, material, contributed reconstruction interface. Ultimately, demonstrated satisfactory simultaneous defects. promising strategy application 3D technology was proposed.

Language: Английский

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Bioengineering strategies targeting angiogenesis: Innovative solutions for osteonecrosis of the femoral head

Journal of Tissue Engineering, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 1, 2025

Osteonecrosis of the femoral head (ONFH) is a prevalent orthopedic disorder characterized primarily by compromised blood supply. This vascular deficit results in cell apoptosis, trabecular bone loss, and structural collapse at late stage, significantly impairing joint function. While MRI highly effective tool for diagnosing ONFH its early stages, challenges remain due to limited availability high cost MRI, as well absence routine screening asymptomatic patients. . In addition, current therapeutic strategies predominantly only relieve symptoms while disease-modifying drugs are still under investigation/development. Considering that supply plays key role pathology ONFH, angiogenic therapies have been put forward promising treatment options. Emerging bioengineering interventions targeting angiogenesis hold potential treatment. this review, we introduce advances research into summarize novel angiogenesis. review sheds light upon new directions future ONFH.

Language: Английский

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Immunomodulation in Bone Tissue Engineering: Recent Advancements in Scaffold Design and Biological Modifications for Enhanced Regeneration

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Bone defects, whether caused by trauma, cancer, infectious diseases, or surgery, can significantly impair people's quality of life. Although autografts are the gold standard for treating bone they often fall short in adequately forming tissue. The field tissue engineering has made strides using scaffolds with various biomaterials, stem cells, and growth factors to enhance healing. However, some biological structures do not yield satisfactory therapeutic outcomes new formation. Recent studies have shed light on crucial role immunomodulation, specifically interaction between implanted scaffold host immune systems, regeneration. Immune particularly macrophages, pivotal inflammatory response, angiogenesis, osteogenesis. This review delves into system's mechanism toward foreign bodies recent advancements scaffolds' physical properties that foster regeneration modulating macrophage polarization an anti-inflammatory phenotype enhancing osteoimmune microenvironment.

Language: Английский

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Static Topographical Cue Combined with Dynamic Fluid Stimulation Enhances the Macrophage Extracellular Vesicle Yield and Therapeutic Potential for Bone Defects

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Extracellular vesicles (EVs) hold promise for tissue regeneration, but their low yield and limited therapeutic efficacy hinder clinical translation. Bioreactors provide a larger culture surface area stable environment large-scale EV production, yet ability to enhance is limited. Physical stimulation, by inducing cell differentiation modulating cargo composition, offers more efficient, cost-effective, reproducible approach compared the loading of EVs biochemical priming parental cells. Herein, effects 3D-printed perfusion bioreactor with topographical cue on macrophage bioactivity were assessed. The results indicate that increased 12.5-fold enhanced in promoting osteogenic angiogenesis via upregulated miR-210-3p. Mechanistically, fluid shear stress activates Piezo1, triggering Ca2+ influx Yes-associated protein (YAP) nuclear translocation, secretion enhancing M2 polarization conjunction morphological changes guided aligned topography. Moreover, porous electrospun membrane-hydrogel composite scaffold loaded bioreactor-derived exhibited outstanding rat cranial defect model. This study presents scalable, platform production EVs, potentially overcoming key challenges translating EV-based therapies clinic.

Language: Английский

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Silk fibroin thermosensitive polymers: Osteogenic, anti-inflammatory, and angiogenic effects for osteomyelitis treatment

Journal of Biomaterials Science Polymer Edition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 17

Published: Feb. 16, 2025

Infectious bone defects pose a significant challenge in orthopedics by hindering healing and vascularization. This study explored the impact of fibroin thermosensitive hydrogel on osteogenesis, inflammatory response, angiogenesis as potential biomaterial for regeneration osteomyelitis treatment. The biocompatibility live/dead staining revealed high number viable osteoblast cells after 14 days. ALP activity was significantly increased all formulations, with F3 showing highest levels total protein content calcium deposition, indicating more effective osteogenesis. Gene expression analysis osteogenesis-related genes demonstrated that RUNX2 upregulated day 7, followed expressions OCN COL-1 at later stages. response to assessed measuring nitric oxide (NO) production pro-inflammatory gene LPS-stimulated RAW 264.7 macrophages. formulation reduced NO iNOS expression, suggesting selective inhibition pathway. VEGF-loaded exhibited substantial angiogenic potential, enhancing HUVEC cell proliferation 140% over 48 h. osteogenic, anti-inflammatory, effects shown were well-suited applications

Language: Английский

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A 3D-printed scaffold composed of Alg/HA/SIS for the treatment of diabetic bone defects

Journal of Orthopaedic Translation, Journal Year: 2024, Volume and Issue: 48, P. 25 - 38

Published: July 26, 2024

Language: Английский

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Applications and Progress of 3D Printed Bioceramic Scaffolds in Bone Tissue Repair and Immune Regulation

Ceramics International, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

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Angiogenesis unveiled: Insights into its role and mechanisms in cartilage injury

Experimental Gerontology, Journal Year: 2024, Volume and Issue: 195, P. 112537 - 112537

Published: Aug. 6, 2024

Osteoarthritis (OA) commonly results in compromised mobility and disability, thereby imposing a significant burden on healthcare systems. Cartilage injury is prevalent pathological manifestation OA constitutes central focus for the development of treatment strategies. Despite considerable number studies aimed at delaying this degenerative process, their outcomes remain unvalidated preclinical settings. Recently, therapeutic strategies focused angiogenesis have attracted growing interest from researchers. Thus, we conducted comprehensive literature review to elucidate current progress research pinpoint gaps domain. Additionally, it provides theoretical guidance future endeavors

Language: Английский

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Functionalized 3D-printed GelMA/Laponite hydrogel scaffold promotes BMSCs recruitment through osteoimmunomodulatory enhance osteogenic via AMPK/mTOR signaling pathway

Materials Today Bio, Journal Year: 2024, Volume and Issue: 29, P. 101261 - 101261

Published: Sept. 23, 2024

Language: Английский

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