Synthesis and Antioxidant Effects of Edaravone-Loaded MPEG-2000-DSPE Micelles in Rotenone-Induced PC12 Cell Model of Parkinson’s Disease DOI Creative Commons
Xin Luo,

Linshan Luo,

Rong Lai

et al.

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(23), P. 1962 - 1962

Published: Dec. 6, 2024

Parkinson's disease (PD) is the second most common neurodegenerative disorder globally that lacks any disease-modifying drug for prevention or treatment. Oxidative stress has been identified as one of key pathogenic drivers (PD). Edaravone, an approved free-radical scavenger, proven to have potential against PD by targeting multiple pathologies, including oxidative stress, focal mitochondria, and neuroinflammation. However, its bioavailability potentially restricted due poor solubility short half-life. This study aims develop a simple effective delivery system edaravone enhance solubility, stability, improve neuroprotective efficacy. An MPEG-2000-DSPE-edaravone (MDE) micelle was prepared via solvent evaporation using MPEG-2000-DSPE carrier encapsulate edaravone. The morphology, particle size, zeta potential, chemical structure, loading MDE were evaluated. We then investigated whether such targeted could provide enhanced neuroprotection. A cell model established in PC12 cells through exposure rotenone. effects on treated with without rotenone evaluated counting kit-8, calcein acetoxymethyl ester (AM)-propidine iodide (PI) staining, flow cytometry. Cell migration wound healing assay. Additionally, intracellular antioxidant performed ROS-level-detecting DCFH-DA probe, mitochondrial membrane potentials JC-1 drug-loading content 17.6% encapsulation efficiency 92.8% successfully prepared. resultant had mean size 112.97 ± 5.54 nm -42 mV. Cytotoxicity assays confirmed (≤200 ug/mL) exhibited promising cytocompatibility no significant effect viability, cycle regulation, apoptosis levels. Likewise, compared free edaravone, noted MDE. might be able target into cells, increasing providing local effect. results demonstrated material enhancing edaravone's aqueous effects. formulation treating other diseases which plays role pathogenesis.

Language: Английский

From micro to macro, nanotechnology demystifies acute pancreatitis: a new generation of treatment options emerges DOI Creative Commons
Wei Du, Xinyue Wang,

Yuyan Zhou

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 29, 2025

Acute pancreatitis (AP) is a disease characterized by an acute inflammatory response in the pancreas. This caused abnormal activation of pancreatic enzymes variety etiologic factors, which results localized response. The symptoms this include abdominal pain, nausea and vomiting fever. These are induced hyperinflammatory oxidative stress. In recent years, research has focused on developing anti-inflammatory antioxidative therapies for treatment (AP). However, there still limitations to approach, including poor drug stability, low bioavailability short half-life. advent nanotechnology opened up novel avenue management Nanomaterials can serve as efficacious vehicle conventional pharmaceuticals, enhancing their targeting ability, improving prolonging Moreover, they also exert direct therapeutic effect. review begins introducing general situation It then discusses pathogenesis current status treatment. Finally, it considers literature related nanomaterials. objective study provide comprehensive existing use nanomaterials particular, changes markers outcomes following administration examined. done with intention offering insights that inform subsequent facilitate clinical application

Language: Английский

Citations

0
Botanical Flavonoids: Efficacy, Absorption, Metabolism and Advanced Pharmaceutical Technology for Improving Bioavailability DOI Creative Commons
Lei Hu, Yongming Luo, Jiaxin Yang

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(5), P. 1184 - 1184

Published: March 6, 2025

Flavonoids represent a class of natural plant secondary metabolites with multiple activities including antioxidant, antitumor, anti-inflammatory, and antimicrobial properties. However, due to their structural characteristics, they often exhibit low bioavailability in vivo. In this review, we focus on the vivo study flavonoids, particularly effects gut microbiome common modifications such as methylation, acetylation, dehydroxylation, etc. These aim change characteristics original substances enhance absorption bioavailability. order improve discuss two feasible methods, namely dosage form modification chemical modification, hope that these approaches will offer new insights into application flavonoids for human health. article, also introduce types, sources, efficacy flavonoids. conclusion, is comprehensive review how

Language: Английский

Citations

0
Microenvironment responsive nanomedicine for acute pancreatitis treatment DOI
Yixuan Zhu, Qian Wang,

Yao-Yao Zheng

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: unknown, P. 114633 - 114633

Published: March 1, 2025

Language: Английский

Citations

0
Inflammation-driven biomimetic nano-polyphenol drug delivery system alleviates severe acute pancreatitis by inhibiting macrophage PANoptosis and pancreatic enzymes oversecretion DOI Creative Commons
Junyong Wu, Hai Huang, W. Xu

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Severe acute pancreatitis (SAP) is a critical inflammatory disease with high morbidity and mortality. Current treatments focused on symptomatic relief but failed to prevent inflammation progression in cellular level. In order develop an SAP-targeting drug delivery system alleviate SAP level illustrate its mechanism, we explored the use of proanthocyanidin (PYD) pentoxifylline (PTX) loaded into macrophage membrane-coated self-assembly nanoparticles (FePTX@CM NPs) for targeted treatment. The combination application these two drugs was innovative aid. We developed NPs by strategy cell membrane coating. Its particle size zeta potential were measured dynamic light scatter (DLS). morphology observed transmission electron microscopy (TEM). And encapsulation efficiency evaluated nano-flow cytometry. total protein profile determined via Coomassie brilliant blue. explore mechanism our against animal levels. Bioinformatics approaches, TEM, immunofluorescent assay co-immunoprecipitation performed comprehensively explain specific anti-SAP FePTX@CM NPs. After inflammation-driven targeting, PYD inhibited pancreatic amylase lipase release suppressing mitochondrial reactive oxygen species (mtROS)/Golgi stress, while PTX prevented SAP-associated PANoptosis inhibiting Zbp1 signal pathway. protection effect biomimetic worked from different aspects symptoms relative cells. demonstrated effective pancreas reduced systemic especially pro-inflammatory recruitment activation, minimized tissue damage mouse models, offering promising therapeutic clinical management.

Language: Английский

Citations

0
Kaempferol Alleviates Hepatic Injury in Nonalcoholic Steatohepatitis (NASH) by Suppressing Neutrophil-Mediated NLRP3-ASC/TMS1-Caspase 3 Signaling DOI Creative Commons
Yang He, Dandan Li, Guolan Gao

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(11), P. 2630 - 2630

Published: June 3, 2024

Non-alcoholic fatty liver disease (NAFLD) is a significant hepatic condition that has gained worldwide attention. Kaempferol (Kae), renowned for its diverse biological activities, including anti-inflammatory, antioxidant, anti-aging, and cardio-protective properties, emerged as potential therapeutic candidate non-alcoholic steatohepatitis (NASH). Despite promising potential, the precise underlying mechanism of Kae's beneficial effects in NASH remains unclear. Therefore, this study aims to clarify by conducting comprehensive vivo vitro experiments.

Language: Английский

Citations

2
Therapeutic potential of plant polyphenols in acute pancreatitis DOI
Chengu Niu, Jing Zhang, Patrick Okolo

et al.

Inflammopharmacology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 4, 2024

Language: Английский

Citations

1
Mitochondrial dysfunction in pancreatic acinar cells: mechanisms and therapeutic strategies in acute pancreatitis DOI Creative Commons
Fan Chen, Kedong Xu,

Yimin Han

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 24, 2024

Acute pancreatitis (AP) is an inflammatory disease of the pancreas and a complex process involving multiple factors, with mitochondrial damage playing crucial role. Mitochondrial dysfunction now considered key driver in development AP. This often presents as increased oxidative stress, altered membrane potential permeability, DNA mutations. Under stress conditions, dynamics ROS production increase, leading to decreased potential, imbalanced calcium homeostasis, activation permeability transition pore. The release (mtDNA), recognized damage-associated molecular patterns, can activate cGAS-STING1 NF-κB pathway induce pro-inflammatory factor expression. Additionally, mtDNA inflammasomes, interleukin subsequent tissue inflammation. review summarizes relationship between mitochondria AP explores protective strategies diagnosis treatment this disease. Future research on acute benefit from exploring promising avenues such antioxidants, inhibitors, new therapies that target dysfunction.

Language: Английский

Citations

1
Synthesis and Antioxidant Effects of Edaravone-Loaded MPEG-2000-DSPE Micelles in Rotenone-Induced PC12 Cell Model of Parkinson’s Disease DOI Creative Commons
Xin Luo,

Linshan Luo,

Rong Lai

et al.

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(23), P. 1962 - 1962

Published: Dec. 6, 2024

Parkinson's disease (PD) is the second most common neurodegenerative disorder globally that lacks any disease-modifying drug for prevention or treatment. Oxidative stress has been identified as one of key pathogenic drivers (PD). Edaravone, an approved free-radical scavenger, proven to have potential against PD by targeting multiple pathologies, including oxidative stress, focal mitochondria, and neuroinflammation. However, its bioavailability potentially restricted due poor solubility short half-life. This study aims develop a simple effective delivery system edaravone enhance solubility, stability, improve neuroprotective efficacy. An MPEG-2000-DSPE-edaravone (MDE) micelle was prepared via solvent evaporation using MPEG-2000-DSPE carrier encapsulate edaravone. The morphology, particle size, zeta potential, chemical structure, loading MDE were evaluated. We then investigated whether such targeted could provide enhanced neuroprotection. A cell model established in PC12 cells through exposure rotenone. effects on treated with without rotenone evaluated counting kit-8, calcein acetoxymethyl ester (AM)-propidine iodide (PI) staining, flow cytometry. Cell migration wound healing assay. Additionally, intracellular antioxidant performed ROS-level-detecting DCFH-DA probe, mitochondrial membrane potentials JC-1 drug-loading content 17.6% encapsulation efficiency 92.8% successfully prepared. resultant had mean size 112.97 ± 5.54 nm -42 mV. Cytotoxicity assays confirmed (≤200 ug/mL) exhibited promising cytocompatibility no significant effect viability, cycle regulation, apoptosis levels. Likewise, compared free edaravone, noted MDE. might be able target into cells, increasing providing local effect. results demonstrated material enhancing edaravone's aqueous effects. formulation treating other diseases which plays role pathogenesis.

Language: Английский

Citations

0