Controlling stem cell fate using cold atmospheric plasma DOI Creative Commons
Fei Tan, Yin Fang, Liwei Zhu

et al.

Stem Cell Research & Therapy, Journal Year: 2020, Volume and Issue: 11(1)

Published: Aug. 26, 2020

Abstract The stem cell is the foundation of regenerative medicine and tissue engineering. Regulating specific fate, such as attachment, proliferation, differentiation, even death, undergoes continuous development. Cold atmospheric plasma (CAP), core technology medicine, attracting tremendous attention due to its ability versatility manipulate various types cells, including cells. Specifically, direct indirect applications CAP in controlling fate are best exemplified by upfront irradiation cells modification niche, respectively. This review will describe recent advances strategies, both indirect, their influence on healthy cancer Particular emphasis be placed mechanism connecting physical chemical cues carried biological changes presented especially at transcriptomic level. ultimate goal exploit CAP’s potential medicine.

Language: Английский

Plasma-Conditioned Liquids as Anticancer Therapies In Vivo: Current State and Future Directions DOI Open Access
Xavi Solé‐Martí, Albert Espona‐Noguera, Maria‐Pau Ginebra

et al.

Cancers, Journal Year: 2021, Volume and Issue: 13(3), P. 452 - 452

Published: Jan. 25, 2021

Plasma-conditioned liquids (PCL) are gaining increasing attention in the medical field, especially oncology, and translation to clinics is advancing on a good path. This emerging technology involving cold plasmas has great potential as therapeutic approach cancer diseases, PCL have been shown selectively kill cells by triggering apoptotic mechanisms without damaging healthy cells. In this context, can be injected near tumor or intratumorally, thereby allowing treatment of malignant tumors located internal organs that not accessible for direct atmospheric plasma (CAP) treatment. Therefore, constitutes very interesting minimally invasive alternative CAP therapy, avoiding surgeries multiple local administrations. As field advances, it progressively moving evaluation effects vivo scenarios. Exciting developments pushing forward clinical novel therapy. However, there still room research, quantification identification reactive oxygen nitrogen species (RONS) conditions yet clarified, dosage regimens highly variable among studies, other more relevant models could used. work aims present critical review state anticancer agents applied studies.

Language: Английский

Citations

42
Plasma bioscience for medicine, agriculture and hygiene applications DOI Open Access
Eun Ha Choi, Nagendra Kumar Kaushik, Young Joon Hong

et al.

Journal of the Korean Physical Society, Journal Year: 2022, Volume and Issue: 80(8), P. 817 - 851

Published: March 4, 2022

Language: Английский

Citations

36
Tea saponins as natural emulsifiers and cryoprotectants to prepare silymarin nanoemulsion DOI Creative Commons
Mao Deng, Huijuan Chen, Long Xie

et al.

LWT, Journal Year: 2022, Volume and Issue: 156, P. 113042 - 113042

Published: Jan. 2, 2022

Tea saponins (TS), a natural surfactant, are extracted from Camellia oleifera seed meal of plants. Silymarin (SM) is flavonoid compound with hepatoprotective effect, and its clinical application limited by insoluble properties low oral bioavailability. The objective this study was to investigate the potential TS as emulsifiers prepare silymarin nanoemulsion (SM-NE) cryoprotectants prevent irreversible aggregation SM-NE droplets during freeze-drying. particle size polydispersity index (PDI) decreased concentration emulsifier-TS increased (particle size: 192–360 nm; PDI: 0.034–0.100). freeze-dried powder (SM-NE-FDP) using 5% (w/v) had good redispersibility. effectively prevented P-XRD patterns indicated that SM successfully encapsulated in TS-stabilized SM-NE. TEM SEM images showed were spherical uniform size. FT-IR analysis confirmed chemical structure not significantly changed. In vitro cumulative release sustained affected pH. studies proved ability SM-NE, reduce damage

Language: Английский

Citations

35
Plasma Dermatology: Skin Therapy Using Cold Atmospheric Plasma DOI Creative Commons
Fei Tan, Yang Wang, Shiqun Zhang

et al.

Frontiers in Oncology, Journal Year: 2022, Volume and Issue: 12

Published: July 12, 2022

Cold atmospheric plasma-based plasma medicine has been expanding the diversity of its specialties. As an emerging branch, dermatology takes advantage beneficial complexity constituents (e.g., reactive oxygen and nitrogen species, UV photons, electromagnetic emission), technical versatility direct irradiation indirect aqueous treatment), practical feasibility hand-held compact device clinician-friendly operation). The objective this comprehensive review is to summarize recent advances in CAP-dominated skin therapy by broadly covering three aspects. We start with optimisation intact skin, detailing effect CAP on lipids, cells, histology, blood circulation. then conduct a clinically oriented thorough dissection treatment various diseases, focusing wound healing, inflammatory disorders, infectious conditions, parasitic infestations, cutaneous malignancies, alopecia. Finally, we conclude brief analysis safety aspect proposal how mitigate potential risks. This endeavors serve as mini textbook for clinical dermatologists manual biotechnologists. Our collective goal consolidate dermatology’s lead modern personalized medicine.

Language: Английский

Citations

32
Nanodelivery systems for cutaneous melanoma treatment DOI Creative Commons
Irina Pereira,

Carina Monteiro,

Miguel Pereira‐Silva

et al.

European Journal of Pharmaceutics and Biopharmaceutics, Journal Year: 2023, Volume and Issue: 184, P. 214 - 247

Published: Feb. 10, 2023

Cutaneous melanoma (CM) is a multifactorial disease whose treatment still presents challenges: the rapid progression to advanced CM, which leads frequent recurrences even after surgical excision and, notably, low response rates and resistance available therapies, particularly in case of unresectable metastatic CM. Thereby, alternative innovative therapeutic approaches for CM continue be searched. In this review we discuss relevant preclinical research studies, provide broad-brush analysis patents clinical trials involve application nanotechnology-based delivery systems therapy. Nanodelivery have been developed anticancer biomolecules can administered by different routes. Overall, nanosystems could promote technological advances several modalities used combinatorial therapies. Nevertheless, results these studies not translated applications. Thus, concerted collaborative involving basic, applied, translational, scientists need performed allow development effective safe nanomedicines treat

Language: Английский

Citations

18
Cold Atmospheric Plasma and Silymarin Nanoemulsion Activate Autophagy in Human Melanoma Cells DOI Open Access
Manish Adhikari, Bhawana Adhikari, Bhagirath Ghimire

et al.

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(6), P. 1939 - 1939

Published: March 12, 2020

Autophagy is reported as a survival or death-promoting pathway that highly debatable in different kinds of cancer. Here, we examined the co-effect cold atmospheric plasma (CAP) and silymarin nanoemulsion (SN) treatment on G-361 human melanoma cells via autophagy induction.The temperature pH media, along with cell number, were evaluated. The intracellular glucose level PI3K/mTOR EGFR downstream pathways assessed. Autophagy-related genes, related transcriptional factors, induction estimated using confocal microscopy, flow cytometry, ELISA.CAP increased while its combination SN resulted decrease ATP downregulation PI3K/AKT/mTOR RAS/MEK pathways. Co-treatment blocked paths reduced PI3K (2 times), mTOR (10 (5 HRAS MEK times). CAP co-treated modulates factor expressions (ZKSCAN3, TFEB, FOXO1, CRTC2, CREBBP) specific genes (BECN-1, AMBRA-1, MAP1LC3A, SQSTM) to induction.CAP together activate by activating pathways, expressing autophagy-related transcription factors genes.

Language: Английский

Citations

50
Dosing: The key to precision plasma oncology DOI
Xiaofeng Dai,

Zhifa Zhang,

Jianying Zhang

et al.

Plasma Processes and Polymers, Journal Year: 2020, Volume and Issue: 17(10)

Published: April 16, 2020

Abstract Cold atmospheric plasma (CAP) is an emerging oncotherapeutic approach with selectivity for cancer cells. It can induce different cell survival and death programs depending on the CAP dose, it function as a neo or adjuvant therapy against cancer. Establishing evaluation system precise dosing key to make act alone in combination other therapeutic modalities achieving desirable treatment responses. By classifying CAP‐induced effects associating them of plasma‐reactive agents, we identify opportunities contribute precision oncotherapy discuss challenges en route clinical applications. We emphasize importance medicine encourage cross‐disciplinary collaborations develop suitable metrics.

Language: Английский

Citations

45
An overview of advanced formulation and nanotechnology-based approaches for solubility and bioavailability enhancement of silymarin DOI

Siddharth S. Kesharwani,

Vikas Jain, Surajit Dey

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2020, Volume and Issue: 60, P. 102021 - 102021

Published: Aug. 20, 2020

Language: Английский

Citations

40
Plasma bioscience and its application to medicine DOI Creative Commons
Eun Ha Choi, Han S. Uhm, Nagendra Kumar Kaushik

et al.

AAPPS bulletin, Journal Year: 2021, Volume and Issue: 31(1)

Published: April 6, 2021

Abstract Nonthermal atmospheric pressure biocompatible plasma (NBP), alternatively called bio-cold plasma, is a partially ionized gas that consists of charged particles, neutral atoms and molecules, photons, an electric field, heat. Recently, nonthermal plasma-based technology has been applied to bioscience, medicine, agriculture, food processing, safety. Various device configurations electrode layouts fast-tracked applications in the treatment biological material surfaces. The NBP action mechanism may be related synergy constituents, such as ultraviolet radiation or reactive species. used inactivation viruses resistant microbes, fungal cells, bacteria, spores, biofilms made by microbes. It also heal wounds, coagulate blood, degrade pollutants, functionalize surfaces, kill cancers, for dental applications. This review provides outline devices their bioscience medicine. We discuss role plasma-activated liquids applications, cancer treatments agriculture. individual adaptation meet specific medical requirements necessitates real-time monitoring both performance target treated will provide new paradigm therapeutic clinical systems.

Language: Английский

Citations

37
Intracellular Responses Triggered by Cold Atmospheric Plasma and Plasma-Activated Media in Cancer Cells DOI Creative Commons
Helena Motaln, Nina Recek, Boris Rogelj

et al.

Molecules, Journal Year: 2021, Volume and Issue: 26(5), P. 1336 - 1336

Published: March 2, 2021

Cold atmospheric plasma (CAP), an ionized gas operating at room temperature, has been increasingly studied with respect to its potential use in medicine, where beneficial effects on tumor reduction oncology have demonstrated. This review discusses the cellular changes appearing cell membranes, cytoplasm, various organelles, and DNA content upon cells’ direct or indirect exposure CAP CAP-activated media/solutions (PAM), respectively. In addition, CAP/PAM impact main processes of proliferation, migration, protein degradation forms death is addressed, especially light field medicine.

Language: Английский

Citations

35