Anti-Cancer Potential of Cannabinoids, Terpenes, and Flavonoids Present in Cannabis

Cancers, Journal Year: 2020, Volume and Issue: 12(7), P. 1985 - 1985

Published: July 21, 2020

In recent years, and even more since its legalization in several jurisdictions, cannabis the endocannabinoid system have received an increasing amount of interest related to their potential exploitation clinical settings. Cannabinoids been suggested shown be effective treatment various conditions. cancer, is altered numerous types tumours can relate cancer prognosis disease outcome. Additionally, cannabinoids display anticancer effects models by suppressing proliferation, migration and/or invasion cells, as well tumour angiogenesis. However, therapeutic use currently limited symptoms pain associated with chemotherapy, while cytotoxic drugs chemotherapy still requires validation patients. Along cannabinoids, contains other compounds that also exert anti-tumorigenic actions. The anti-cancer terpenes flavonoids, present cannabis, are explored this literature review.

Language: Английский

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Low-Temperature Plasma for Biology, Hygiene, and Medicine: Perspective and Roadmap

IEEE Transactions on Radiation and Plasma Medical Sciences, Journal Year: 2021, Volume and Issue: 6(2), P. 127 - 157

Published: Dec. 14, 2021

Plasma, the fourth and most pervasive state of matter in visible universe, is a fascinating medium that connected to beginning our universe itself. Man-made plasmas are at core many technological advances include fabrication semiconductor devices, which enabled modern computer communication revolutions. The introduction low temperature, atmospheric pressure biomedical field has ushered new revolution healthcare arena promises introduce plasma-based therapies combat some thorny long-standing medical challenges. This article presents an overview where research today discusses innovative concepts approaches overcome present challenges take next level. It written by team experts who took in-depth look various applications plasma hygiene, decontamination, medicine, made critical analysis, proposed ideas should help community focus their efforts on clear practical steps necessary keep advancing for decades come.

Language: Английский

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Critical review of therapeutic potential of silymarin in cancer: A bioactive polyphenolic flavonoid

Journal of Functional Foods, Journal Year: 2023, Volume and Issue: 104, P. 105502 - 105502

Published: March 21, 2023

Cancer is identified as second leading causes of death and imposes a significant psychological economic burden on societies. Due to its high prevalence, main cause worldwide, there continuing need discover new potential anticancer compounds. Plant-derived compounds have recently attracted more attention among researchers for their potent anti-cancer properties. A numerous research, including in-vitro, in-vivo, preclinical, clinical, epidemiological studies, has indicated that the flavonoid silymarin comprehensive preventive therapeutic effects array inflammation-driven diseases, cancer. Silymarin clear activities through distinctive mechanisms modulation apoptosis in-vitro survival in-vivo. The chemoprotection constituent suggest they could be promising agents in various cancer types. This review focus vivo vitro variety cancers, explores signaling pathways modulated by this compound.

Language: Английский

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Piezoeletric cold atmospheric plasma induces apoptosis and autophagy in human hepatocellular carcinoma cells through blocking glycolysis and AKT/mTOR/HIF-1α pathway

Free Radical Biology and Medicine, Journal Year: 2023, Volume and Issue: 208, P. 134 - 152

Published: Aug. 4, 2023

Hepatocellular carcinoma (HCC) is the sixth most prevalent cancer and fourth leading cause of cancer-related death worldwide. Advanced or metastatic HCC currently managed using systemic drug therapy with unsatisfactory patient survival. Cold atmospheric plasma has emerged as a promising, physicochemical, broad-spectrum oncotherapy. In this preclinical study, we investigated anti-neoplastic functions mechanism piezoelectric direct discharge technology-based CAP, Piezo-CAP, on in vitro vivo. Various cells lines, such SMMC7721, HepG2 LM3, were used model for phenotypic mechanistic studies. Specifically, cell counting Kit-8 colony formation assay, flow cytometry, Transwell Western blot, reactive oxygen species (ROS) glutathione to oxidized ratio (GSH/GSSG) assay demonstrate plasma-induced changes proliferation, cycle progression, migration invasion, epithelial-to-mesenchymal transition, intracellular ROS, antioxidant capacity, respectively. addition, Acridine orange ethidium bromide (AO/EB) staining transmission electron microscopy performed cellular subcellular assessment apoptosis. The Ad-mCherry-RFP-LC3B fluorescent double-labeled lentiviral system was detect autophagic flux. On other hand, RNA-sequencing, quantitative real-time PCR, blot metabolic molecular disruption tumor glycolysis oncogenic vivo experiments human cell-line-derived xenograft immunohistochemistry (IHC) utilized investigate mechanism. Piezo-CAP exerted through inhibiting promote apoptosis autophagy. Treatment could suppress proliferation induce autophagy simultaneously disrupts survival pathways redox deregulation, glycolytic pathway, PI3K/AKT/mTOR/HIF1α pathway signaling. Moreover, upon translation these results into tissue level, significantly suppressed situ growth. These findings collectively suggest that Piezo-CAP-induced though multitargeted blockade major deregulated balance, glycolysis, PI3K/AKT/mTOR/HIF-1α

Language: Английский

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The Promising Potential of Cold Atmospheric Plasma Therapies

Plasma, Journal Year: 2024, Volume and Issue: 7(2), P. 465 - 497

Published: June 12, 2024

The outstanding properties and chemistry of cold atmospheric plasma (CAP) are not sufficiently understood due to their relatively complex systems transient properties. In this paper, we tried present a detailed review the applications CAP in modern medicine, highlighting biochemistry phenomenon. Due its unique characteristics, has emerged as promising tool various medical applications. CAP, partially—or fully ionized—gas-retaining state quasi-neutrality, contains many particles, such electrons, charged atoms, molecules displaying collective behaviour caused by Coulomb interactions. can be generated at pressure, making it suitable for settings. Cold plasma’s anti-microbial create an alternative method antibiotics when treating infections. It also enhances cell proliferation, migration, differentiation, leading accelerated tissue regeneration. powerful anti-tumour therapies, stem dental applications, disease treatment, e.g., neurology. is our belief that article contributes deeper understanding therapy potential medicine. objective study demonstrate novel approach treatment modality. By covering range biomedical fields, hope provide comprehensive overview multiple conditions. order gain further insight into subject, attempted gather crucial research evidence from studies, hopefully creating compelling argument favour therapy. Our aim highlight innovative aspects where traditional methods may have limitations. Through article, intend convenient reference source readers engaged examination CAP’s

Language: Английский

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Cold atmospheric plasma and silymarin nanoemulsion synergistically inhibits human melanoma tumorigenesis via targeting HGF/c-MET downstream pathway

Cell Communication and Signaling, Journal Year: 2019, Volume and Issue: 17(1)

Published: May 24, 2019

Recent studies claimed the important role of cold atmospheric plasma (CAP) with nanotechnology in cancer treatments. In this study, silymarin nanoemulsion (SN) was used along air CAP as therapeutic agent to counter human melanoma. we examined combined treatment and SN on G-361 melanoma cells by evaluating cellular toxicity levels, reactive oxygen nitrogen species (RONS) DNA damage, melanoma-specific markers, apoptosis, caspases poly ADP-ribose polymerase-1 (PARP-1) levels using flow cytometer. Dual-treatment effects epithelial–mesenchymal transition (EMT), Hepatocyte growth factor (HGF/c-MET) pathway, sphere formation reversal EMT were also assessed western blotting microscopy respectively. plasma-activated medium (PAM) applied tumor body weight markers mesenchymal xenograft nude mice model checked. Co-treatment increased a time-dependent manner shows maximum at 200 nM 24 h. Intracellular RONS showed significant generation ROS (< 3 times) RNS 2.5 dual-treated samples compared control. damage estimating level γ-H2AX (1.8 times), PD-1 (> 2 DNMT cells. Increase Caspase 8,9,3/7 1.5 PARP (2.5 apoptotic genes observed dual treated group hence blocking HGF/c-MET pathway. Decrease (E-cadherin, YKL-40, N-cadherin, SNAI1) seen simultaneously decline (BRAF, NAMPT) stem (CD133, ABCB5) markers. vivo results reduction PAM size. The use μ-DBD can minimize malignancy describing molecular mechanism acting xenografts, possibly leading suitable targets for innovative anti-melanoma approaches future.

Language: Английский

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Cold Physical Plasma in Cancer Therapy: Mechanisms, Signaling, and Immunity

Oxidative Medicine and Cellular Longevity, Journal Year: 2021, Volume and Issue: 2021(1)

Published: Jan. 1, 2021

Despite recent advances in therapy, cancer still is a devastating and life‐threatening disease, motivating novel research lines oncology. Cold physical plasma, partially ionized gas, new modality research. Physical plasma produces various physicochemical factors, primarily reactive oxygen nitrogen species (ROS/RNS), causing cell death when supplied at supraphysiological concentrations. This review outlines the biomedical consequences of treatment experimental including modalities. It also summarizes current knowledge on intracellular signaling pathways triggered by to induce death. Besides inactivation tumor cells, an equally important aspect inflammatory context which occurs suppress or promote responses immune cells. mainly governed release damage‐associated molecular patterns (DAMPs) provoke immunogenic (ICD) that, turn, activates cells innate system adaptive antitumor immunity. The pivotal role treatment, general, highlighted many clinical trials success stories using checkpoint immunotherapy. Hence, potential ICD immunity targeting lesions systemically discussed.

Language: Английский

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Cold atmospheric plasma cancer treatment, direct versus indirect approaches

Materials Advances, Journal Year: 2020, Volume and Issue: 1(6), P. 1494 - 1505

Published: Jan. 1, 2020

This review summarized the unique cellular responses to direct CAP treatment on cancer cells.

Language: Английский

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Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells

Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)

Published: July 20, 2021

The aim of the present study was to investigate combined effects cold atmospheric plasma (CAP) and chemotherapeutic drug doxorubicin (DOX) on murine human melanoma cells, normal cells. In addition free drug, combination CAP with a liposomal (DOX-LIP) also studied for first time. Thiazolyl blue tetrazolium bromide (MTT) Trypan Blue exclusion assays were used evaluate cell viability; mechanism death evaluated by flow cytometry. Combined treatment clonogenic capability tested soft agar colony formation assay. Furthermore effect cellular uptake DOX or DOX-LIP examined. Results showed strong synergistic selectively decreasing viability accelerated apoptotic (or DOX-LIP) dramatically reduced aggressiveness as significantly decreased their anchorage independent growth. Moreover, did not result in increased under experimental conditions. conclusion, facilitates cytotoxic affects metastatic potential reducing clonogenicity, shown

Language: Английский

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An overview of advanced formulation and nanotechnology-based approaches for solubility and bioavailability enhancement of silymarin

Journal of Drug Delivery Science and Technology, Journal Year: 2020, Volume and Issue: 60, P. 102021 - 102021

Published: Aug. 20, 2020

Language: Английский

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