Identification of intestinal microbiome associated with lymph-vascular invasion in colorectal cancer patients and predictive label construction DOI Creative Commons
Chuanbin Chen, Kang Chen,

Zigui Huang

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: May 12, 2023

Objective To identify differences between the composition, abundance, and biological function of intestinal microbiome patients with without lymph-vascular invasion (LVI) colorectal cancer (CRC) to construct predictive labels support accurate assessment LVI in CRC. Method 134 CRC were included, which divided into two groups according presence or absence LVI, their microbiomes sequenced by 16SrRNA analyzed for differences. The transcriptome sequencing data 9 transformed immune cells abundance matrix CIBERSORT algorithm, correlation among LVI-associated differential microbiomes, cells, immune-related genes GO items KEGG pathways analyzed. A random forest (RF) eXtreme Gradient Boosting (XGB) model constructed predict based on microbiome. Result There was no significant difference α-diversity β-diversity (P > 0.05). Linear discriminant analysis Effect Size (LEfSe) showed 34 enriched group 5 significantly non-lymph-vascular (NLVI) group. RF XGB prediction models top 15% ranked feature significance had good efficacy. Conclusions are 39 flora different species NLVI groups. g:Alistipes.s:Alistipes_indistinctus is closely associated vascular invasion. may be involved regulating infiltration influencing expression genes. influence process through a number potential functions. microbial markers gut can used CRC-LVI conditions.

Language: Английский

Interactive Relationships between Intestinal Flora and Bile Acids DOI Open Access

Xiaohua Guo,

Edozie Samuel Okpara,

Wanting Hu

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(15), P. 8343 - 8343

Published: July 28, 2022

The digestive tract is replete with complex and diverse microbial communities that are important for the regulation of multiple pathophysiological processes in humans animals, particularly those involved maintenance intestinal homeostasis, immunity, inflammation, tumorigenesis. diversity bile acids a result joint efforts host microflora. There bidirectional relationship between community that, while flora tightly modulates metabolism synthesis acids, acid pool composition affect homeostasis flora. Homeostatic imbalances systems may lead to development variety diseases, such as inflammatory bowel disease (IBD), colorectal cancer (CRC), hepatocellular carcinoma (HCC), type 2 diabetes (T2DM), polycystic ovary syndrome (PCOS). interactions be (in)directly pathogenesis these diseases.

Language: Английский

Citations

95
Lactobacillus rhamnosus GG cell-free supernatant as a novel anti-cancer adjuvant DOI Creative Commons
Rossella Salemi, Silvia Vivarelli,

Daria Ricci

et al.

Journal of Translational Medicine, Journal Year: 2023, Volume and Issue: 21(1)

Published: March 14, 2023

Abstract Background Gut microbiota modulation has been demonstrated to be effective in protecting patients against detrimental effects of anti-cancer therapies, as well improve the efficacy certain treatments. Among most characterized probiotics, Lactobacillus rhamnosus GG (LGG) is currently utilized clinics alleviate diarrhea, mucositis or intestinal damage which might associated with several triggers, including Clostridium difficile infections, inflammatory gut diseases, antibiotic consumption, chemotherapy radiation therapy. Here, we investigate whether LGG cell-free supernatant (LGG-SN) exert anti-proliferative activity toward colon cancer and metastatic melanoma cells. Moreover, assess potential adjuvant effect LGG-SN combination drugs. Methods alone either 5-Fuorouracil Irinotecan was used treat human cell lines. Dimethylimidazol-diphenyl tetrazolium bromide assay employed detect cellular viability. Trypan blue staining, anti-cleaved caspase-3 anti-total versus PARP western blots, annexin V/propidium iodide flow cytometry analyses were death. Flow measurement DNA content (with propidium staining) together qPCR analysis cyclins expression cycle. Results We demonstrate that able selectively reduce viability cells a concentration-dependent way. While does not any on control fibroblasts. In cells, reduction apoptosis induction, but mitotic arrest G2/M phase Additionally, sensitizes both 5-Fluorouracil Irinotecan, thereby showing positive synergistic action. Conclusion Overall, our results suggest may contain one more bioactive molecules sensitize chemotherapeutic Thus, could proposed an ideal candidate for ground-breaking integrated approaches oncology, chemotherapy-related side overcome resistance relapse issues, thus ameliorating therapeutic response patients.

Language: Английский

Citations

31
Gut Microbiota–Gut Metabolites and Clostridioides difficile Infection: Approaching Sustainable Solutions for Therapy DOI Creative Commons

Bijay Gurung,

Maranda Stricklin,

Shaohua Wang

et al.

Metabolites, Journal Year: 2024, Volume and Issue: 14(1), P. 74 - 74

Published: Jan. 22, 2024

(

Citations

14
A Peptide‐Derived Aggregation‐Induced Emission Nanobioprobe: Unlocking Selective Detection of Fusobacterium nucleatum and Noninvasive Screening of Colorectal Cancer DOI Creative Commons
Hongyu Liu,

T.-C. Wu,

Yunjian Yu

et al.

Aggregate, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

ABSTRACT Colorectal cancer (CRC) screening and early diagnosis is an effective strategy for reducing CRC mortality. However, the current detection methods involve exorbitant costs complex procedures, which are inconvenient large‐scale screening. Given its high prevalence in malignant tissues feces of patients, Fusobacterium nucleatum ( F. ) has emerged as a crucial biomarker CRC. Herein, we propose ‐specific recognition diagnosis. A novel nanobioprobe (AIE‐Pep) with aggregation‐induced emission (AIE) characteristics was synthesized by conjugating red/near‐infrared (NIR) emissive AIE luminogen (AIEgen) FadA‐targeting peptide (ASANWTIQYND). The robust binding affinity between FadA on allows AIE‐Pep NPs to adhere selectively , emits strong red/NIR fluorescence. In model orthotopic CRC, can precisely localize around Moreover, demonstrated limit (LOD) 82.97 CFU/mL could significantly differentiate mice from those normal mice. Overall, this study presents pivotal approach specifically identifying holds immense potential

Language: Английский

Citations

1
Mechanisms of microbiota modulation: Implications for health, disease, and therapeutic interventions DOI Creative Commons
Okechukwu Paul-Chima Ugwu, Esther Ugo Alum, Michael Ben Okon

et al.

Medicine, Journal Year: 2024, Volume and Issue: 103(19), P. e38088 - e38088

Published: May 10, 2024

Microbiota modulation, the intentional change in structure and function of microbial community, is an emerging trajectory that holds promise to mitigate infinite number health issues. The present review illustrates underlying principles microbiota modulation various applications this fundamental process human health, healthcare management, pharmacologic interventions. Different strategies, directing on dietary interventions, fecal transplantation, treatment with antibiotics, bacteriophages, microbiome engineering, immune system, are described detail. This therapeutic implication reflected clinical gastrointestinal disorders immune-mediated diseases for microbiota-modulating agents. In addition this, outlines challenges translating researched outcomes into practice consider safety provides insights future research directions rapidly developing area.

Language: Английский

Citations

8
The footprint of gut microbiota in gallbladder cancer: a mechanistic review DOI Creative Commons
Shujie Liu, Weijian Li, Jun Chen

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: May 7, 2024

Gallbladder cancer (GBC) is the most common malignant tumor of biliary system with worst prognosis. Even after radical surgery, majority patients GBC have difficulty achieving a clinical cure. The risk recurrence remains more than 65%, and overall 5-year survival rate less 5%. gut microbiota refers to variety microorganisms living in human intestine, including bacteria, viruses fungi, which profoundly affect host state general health, disease even cancer. Over past few decades, substantial evidence has supported that plays critical role promoting progression GBC. In this review, we summarize functions, molecular mechanisms recent advances intestinal We focus on driving bacteria pivotal pathways, such as virulence factors, metabolites derived from chronic inflammatory responses ecological niche remodeling. Additionally, emphasize high level correlation between especially EBV Candida spp., general, review not only provides solid theoretical basis for close relationship but also highlights potential research directions further future.

Language: Английский

Citations

7
Unraveling the complexities of colorectal cancer and its promising therapies – An updated review DOI

Sayan Saha,

Shreya Ghosh, Suman Ghosh

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 143, P. 113325 - 113325

Published: Oct. 14, 2024

Language: Английский

Citations

7
Effects of prebiotics, probiotics, and synbiotics on the prevention and treatment of cervical cancer: Mexican consensus and recommendations DOI Creative Commons

Gabriela Gutiérrez Salmeán,

Merari Delgadillo González,

Ariadna Alejandra Rueda Escalona

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: March 28, 2024

Gut microbiota plays a crucial role in modulating immune responses, including effector response to infection and surveillance of tumors. This article summarizes the current scientific evidence on effects supplementation with prebiotics, probiotics, synbiotics high-risk human papillomavirus (HPV) infections, precancerous lesions, various stages cervical cancer development treatment while also examining underlying molecular pathways involved. Our findings indicate that higher dietary fiber intake is associated reduced risk HPV infection, certain probiotics have shown promising results clearing HPV-related lesions. Additionally, strains prebiotics such as inulin fructo-oligosaccharides, decrease frequency gastrointestinal adverse patients. These agents attain their by metabolic pathways, reduction inflammation oxidative stress, promoting apoptosis, inhibiting cell proliferation, suppressing activity oncogenes, thus attenuating tumorigenesis. We conclude although further studies are necessary, robust preclinical models demonstrates play an essential cancer, from carcinogenesis its medical treatment. Consequently, we strongly recommend conducting high-quality clinical trials using these adjuvants since they proven safe.

Language: Английский

Citations

6
Epigenetics and environmental health DOI
Min Zhang, Ting Hu,

Tianyu Ma

et al.

Frontiers of Medicine, Journal Year: 2024, Volume and Issue: 18(4), P. 571 - 596

Published: May 28, 2024

Language: Английский

Citations

5
New Insights into the Relationship between Gut Microbiota and Radiotherapy for Cancer DOI Open Access
Zhipeng Li,

Xiyang Ke,

Zuo Dan

et al.

Nutrients, Journal Year: 2022, Volume and Issue: 15(1), P. 48 - 48

Published: Dec. 22, 2022

Cancer is the second most common cause of death among humans in world, and threat that it presents to human health becoming more serious. The mechanisms cancer development have not yet been fully elucidated, new therapies are changing with each passing day. Evidence from literature has validated finding composition modification gut microbiota play an important role many different types cancer. results also demonstrate there a bidirectional interaction between radiotherapy treatments for In nutshell, modifications caused by effect on tumor radiosensitivity and, as result, affect efficacy show certain radiation toxicity, which leads numerous side effects. What research significance “gut-organ axis” formed may be one interesting potential mechanisms, although relevant still very limited. this review, we combine insights into relationship microbiota, cancer, radiotherapy. Based our current comprehensive understanding relationship, give overview based microbiota.

Language: Английский

Citations

22