Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
Language: Английский
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(12), P. 6591 - 6591
Published: June 15, 2024
Senescence is a physiological and pathological cellular program triggered by various types of stress. Senescent cells exhibit multiple characteristic changes. Among them, the flattened enlarged morphology exhibited in senescent observed regardless stimuli causing senescence. Several studies have provided important insights into pro-adhesive properties senescence, suggesting that cell adhesion to extracellular matrix (ECM), which involved morphological changes, may play pivotal roles Matricellular proteins, group structurally unrelated ECM molecules are secreted environment, unique ability control binding receptors, including integrins. Recent reports certified matricellular proteins closely Through this biological function, thought pathogenesis age-related diseases, fibrosis, osteoarthritis, intervertebral disc degeneration, atherosclerosis, cancer. This review outlines recent on role inducing We highlight integrin-mediated signaling senescence provide new therapeutic options for diseases targeting
Language: Английский
Citations
3
International Immunopharmacology, Journal Year: 2024, Volume and Issue: 139, P. 112666 - 112666
Published: July 12, 2024
Immunotherapy has limited response rates in colorectal cancer (CRC) due to an immunosuppressive tumor microenvironment (TME). Combining transcriptome sequencing, clinical specimens, and functional experiments, we identified a unique group of CAF subpopulations (COX4I2 + ) with inhibited mitochondrial respiration enhanced glycolysis. Through bioinformatics predictions luciferase reporter assays, determined that EBF1 can upstreamly regulate COX4I2 transcription. CAFs functionally phenotypically resemble myofibroblasts, are important for the formation fibrotic TME, capable activating M2 phenotype macrophages. In vitro experiments demonstrated promote TME by blocking CD8 T cell infiltration inducing dysfunction. Using multiple independent cohorts, also found strong correlation between immunotherapy rate CRC patients expression their tumors. Our results identify subpopulation characterized activation EBF1-COX4I2 axis, this be targeted improve outcomes.
Language: Английский
Citations
2
Trends in biotechnology, Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 1, 2024
Language: Английский
Citations
2
Results and problems in cell differentiation, Journal Year: 2024, Volume and Issue: unknown, P. 55 - 87
Published: Jan. 1, 2024
Language: Английский
Citations
1
International Immunopharmacology, Journal Year: 2024, Volume and Issue: 144, P. 113628 - 113628
Published: Nov. 19, 2024
Language: Английский
Citations
1
IEEE Reviews in Biomedical Engineering, Journal Year: 2024, Volume and Issue: 18, P. 231 - 249
Published: Nov. 22, 2024
Immunomechanobiology, the study of how physical forces influence behavior and function immune cells, is a rapidly growing area research. It becoming increasingly recognized that mechanical stimuli, such as fluid shear forces, are critical determinant cell regulation. In this review, we discuss principles significance various present within human body, with focus on flow its impact activation function. Moreover, engineering approaches used to mechanobiology, their implications in health diseases cancer, autoimmune disorders, infectious disease.
Language: Английский
Citations
1
Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)
Published: Sept. 5, 2024
Language: Английский
Citations
0
Hepatology Communications, Journal Year: 2024, Volume and Issue: 8(11)
Published: Oct. 29, 2024
HCC, the most common type of primary liver cancer, is a leading cause cancer-related mortality worldwide. Although advancement immunotherapies by immune checkpoint inhibitors (ICIs) that target programmed cell death 1 or 1-ligand has revolutionized treatment for majority still not beneficial. Accumulating evidence pointed out potent immunosuppressive tumor microenvironment in HCC poses great challenge to ICI therapeutic efficacy. As key component microenvironment, tumor-associated macrophages (TAMs) play vital roles development, progression, and low responsiveness. Mechanistically, TAM can promote cancer invasion metastasis, angiogenesis, epithelial-mesenchymal transition, maintenance stemness, importantly, immunosuppression. Targeting TAMs, therefore, represents an opportunity enhance efficacy patients with HCC. While previous research primarily focused on biochemical cues influencing macrophages, emerging highlights critical role biophysical signals, such as substrate stiffness, topography, external forces. In this review, we summarize influence characteristics within regulate phenotype function TAMs pathogenesis progression. We also explore possible mechanisms discuss potential manipulating regulating therapy. By gaining deeper understanding how sense respond mechanical forces, may potentially usher path toward curative approach combinatory immunotherapies.
Language: Английский
Citations
0
Published: Dec. 19, 2024
Progress in our knowledge of tumor regulatory mechanisms and complexity led to the understanding physical parameters cancer cells their microenvironment, including mechanical, thermal, electrical properties, solid stress, liquid pressure, as critical regulators progression potential prognostic markers associated with clinical outcomes. The biological hallmarks abnormalities tumors are mutually reinforced, promoting a vicious cycle progression. A comprehensive analysis is for developing more robust diagnostic improving treatment efficiency. Like traits, features characterized by inter- intratumoral heterogeneity. dynamic changes properties during result highlight necessity spatial temporal settings. This review focuses on basis tumor-specific state-of-the-art methods analyses, perspective translation. importance disease therapy resistance, well current strategies monitor target traits clinics, highlighted.
Language: Английский
Citations
0
PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0314227 - e0314227
Published: Dec. 19, 2024
Cancer-associated fibroblasts (CAFs) play pivotal roles in solid tumor initiation, growth, and immune evasion. However, the optimal biomimetic modeling conditions remain elusive. In this study, we investigated effects of 2D 3D culturing on human primary CAFs integrated into a modular microenvironment (TME). Using single-nucleus RNA sequencing (snRNAseq) Proteomics’ Proximity Extension Assays, characterized CAF transcriptomic profiles cytokine levels. Remarkably, when cultured 2D, exhibited myofibroblast (myCAF) subtype, whereas spheroid cultures, displayed more inflammatory (iCAF) pathological state. By integrating single-cell gene expression data with functional interrogations critical TME-related processes [natural killer (NK)-mediated killing, monocyte migration, macrophage differentiation], were able to reconcile form function. TME models, enhance cancer cell growth immunologically shield cells from NK cell-mediated cytotoxicity, striking contrast their counterparts. Notably, CAF-secreted proteins manifest immunosuppressive profile by enhancing transendothelial migration differentiation M2-like tumor-associated macrophages (TAMs). Our findings reveal clinically relevant desmoplastic model that can be employed industrial drug discovery campaigns expand cellular target range chemotherapeutics.
Language: Английский
Citations
0