MAPKAPK5-AS1 drives the progression of hepatocellular carcinoma via regulating miR-429/ZEB1 axis

BMC Molecular and Cell Biology, Journal Year: 2022, Volume and Issue: 23(1)

Published: April 25, 2022

Hepatocellular carcinoma (HCC) is a common malignancy. Long non-coding RNAs (lncRNAs) partake in the progression of HCC. However, role lncRNA MAPKAPK5-AS1 development HCC has not been fully clarified.RNA sequencing data and quantitative real-time polymerase chain reaction (qRT-PCR) were adopted to analyze MAPKAPK5-AS1, miR-429 ZEB1 mRNA expressions tissues cell lines. Western blot was used detect ZEB1, E-cadherin N-cadherin protein expressions. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Transwell flow cytometry assays effects on proliferation, migration, invasion apoptosis. Besides, luciferase reporter assay targeting relationship between or 3'UTR. The xenograft tumor mouse models explore effect lung metastasis cells.MAPKAPK5-AS1 up-regulated tissues, expression down-regulated tissues. knockdown could significantly impede epithelial-mesenchymal transition (EMT), as well promote overexpression enhance L02 EMT, inhibit MiR-429 validated be target inhibitors partially offset knocking down cells. positively regulate through repressing miR-429. Moreover, fewer metastatic nodules observed nude mice when knocked can adsorb participate

Language: Английский

---

Molecular regulation of hypoxia through the lenses of noncoding RNAs and epitranscriptome

Wiley Interdisciplinary Reviews - RNA, Journal Year: 2022, Volume and Issue: 14(2)

Published: July 3, 2022

Abstract Cells maintain homeostasis in response to environmental stress through specific cell responses. Hypoxic stress, well known be associated with diverse solid tumors, is one of the main reasons for cancer‐related mortality. Although cells can balance themselves during hypoxic underlying molecular mechanisms are not understood. The enhanced appreciation roles played by noncoding transcriptome and epigenome recent years has brought light involvement RNAs epigenetic modifiers regulation. emergence techniques like deep sequencing facilitated identification large numbers long (lncRNAs) that differentially regulated various cancers. Similarly, proteomic studies have identified such as HATs, HDACs, DNMTs, polycomb groups proteins, their possible regulation hypoxia. crosstalk between lncRNAs play a pivotal role hypoxia‐induced cancer initiation progression. Besides lncRNAs, several other circular RNAs, miRNAs, so forth also expressed conditions. Hypoxia profound effect on expression modifiers. Conversely, RNAs/epigenetic modifies regulate hypoxia signaling axis modulating stability hypoxia‐inducible factors (HIFs). focus this review illustrate orchestration biology, especially cancers, which help identifying promising therapeutic targets This article categorized under: RNA Turnover Surveillance > Regulation Stability Disease Development Structure Dynamics Structure, Chemistry

Language: Английский

---

The hsa_circ_0000276-ceRNA regulatory network and immune infiltration in cervical cancer

BMC Cancer, Journal Year: 2023, Volume and Issue: 23(1)

Published: March 9, 2023

Our previous studies have confirmed that miR-154-5p can regulate pRb expression, and thus, play a tumor suppressor role in HPV16 E7-induced cervical cancer. However, its upstream molecules not been elucidated the progression of This study aimed to explore molecule, hsa_circ_0000276 cancer development possible mechanisms action.We detected differences whole transcriptome expression profiles squamous carcinoma tissues adjacent from patients using microarray technology predict circular RNAs (circRNAs) with binding sites miR-154-5p. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used detect (which had strongest capacity miR-154 selected as target molecule) tissues, followed by vitro functional assays. Downstream microRNAs (miRNAs) mRNAs were identified data databases, while protein-protein interaction networks obtained STRING. A competing endogenous RNA (ceRNA) network centered on constructed Cytoscape GO KEGG databases. Abnormal prognosis critical downstream analyzed gene databases molecular experiments. qRT-PCR western blot analysis performed verify candidate genes.We 4,001 differentially expressed circRNAs between HPV16-positive benign 760 targeting miR-154-5p, including hsa_circ_0000276. directly bound, upregulated, precancerous lesions cells. Silencing inhibited G1/S transition cell proliferation promoted apoptosis SiHa CaSki Bioinformatics showed ceRNA included 17 miRNAs seven mRNAs, upregulated tissues. These associated poor affected cancer-associated immune infiltration. Of these, CD47, LDHA, PDIA3, SLC16A1 downregulated sh_hsa_circ_0000276 cells.Our findings show exerts cancer-promoting effects is an underlying biomarker for carcinoma.

Language: Английский

---

HIF-1-Induced hsa-miR-429: Understanding Its Direct Targets as the Key to Developing Cancer Diagnostics and Therapies

Cancers, Journal Year: 2023, Volume and Issue: 15(11), P. 2903 - 2903

Published: May 25, 2023

MicroRNAs (miRNAs) play a critical role in the regulation of mRNA stability and translation. In spite our present knowledge on mechanisms by miRNAs, utilization translation these ncRNAs into clinical applications have been problematic. Using hsa-miR-429 as an example, we discuss limitations encountered development efficient miRNA-related therapies diagnostic approaches. The miR-200 family members, which include hsa-miR-429, shown to be dysregulated different types cancer. Although members function suppressing epithelial-to-mesenchymal transition, tumor metastasis, chemoresistance, experimental results often contradictory. These complications involve not only complex networks involving noncoding RNAs, but also problem identifying false positives. To overcome limitations, more comprehensive research strategy is needed increase understanding underlying their biological regulation. Here, provide literature analysis verified targets various human models. A meta-analysis this work presented better insights cancer diagnosis any potential therapeutic approach.

Language: Английский

---

Lnc-LRRTM4 promotes proliferation, metastasis and EMT of colorectal cancer through activating LRRTM4 transcription

Cancer Cell International, Journal Year: 2023, Volume and Issue: 23(1)

Published: July 19, 2023

Abstract Numerous mechanisms have shown that long noncoding RNAs (lncRNAs) promote the development of colorectal cancer (CRC), but role lnc-LRRTM4 in progression CRC remains unclear. In this article, we found was highly expressed tissues and cell lines could proliferation metastasis cells. These consequences were achieved by directly binding to promoter LRRTM4 induce its transcription. Moreover, enhanced growth cells vivo promoting cycle reducing apoptosis. Taken together, our results revealed promotes cells, suggesting it may be a potential diagnostic therapeutic target for CRC.

Language: Английский

---