Unveiling the molecular profile of a prostate carcinoma: implications for personalized medicine DOI Creative Commons
Massimiliano Agostini, Erica Giacobbi, Francesca Servadei

et al.

Biology Direct, Journal Year: 2024, Volume and Issue: 19(1)

Published: Dec. 31, 2024

Prostate cancer is the most common diagnosed tumor and fifth related death among men in Europe. Although several genetic alterations such as ERG-TMPRSS2 fusion, MYC amplification, PTEN deletion mutations p53 BRCA2 genes play a key role pathogenesis of prostate cancer, specific gene alteration signature that could distinguish indolent from aggressive or may aid patient stratification for prognosis and/or clinical management patients with still missing. Therefore, here, by multi-omics approach we describe carrying fusion TMPRSS2 ERG 16q chromosome arm. We have observed KDM6A gene, which represent an additional genomic to be considered stratification. The hallmarks signatures highlight intriguing molecular aspects characterize biology this both high hypoxia immune infiltration scores. Moreover, our analysis showed slight increase Tumoral Mutational Burden, well over-expression checkpoints. omics profiling integrating hypoxia, ROS anti-cancer response, optimizes therapeutic strategies advances personalized care patients. here data reported can lay foundation predicting poor studied possibility targeted therapies based on modulation ROS, response.

Language: Английский

Inflammation in the Peripheral Nervous System after Injury DOI Creative Commons

Dandan Gu,

Yiming Xia,

Zihan Ding

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(6), P. 1256 - 1256

Published: June 5, 2024

Nerve injury is a common condition that occurs as result of trauma, iatrogenic injury, or long-lasting stimulation. Unlike the central nervous system (CNS), peripheral (PNS) has strong capacity for self-repair and regeneration. Peripheral nerve results in degeneration distal axons myelin sheaths. Macrophages Schwann cells (SCs) can phagocytose damaged cells. Wallerian (WD) makes whole axon structure degenerate, creating favorable regenerative environment new axons. After macrophages, neutrophils other are mobilized recruited to site necrotic debris. Pro-inflammatory anti-inflammatory factors involved inflammatory response provide microenvironment regeneration regulate effects inflammation on body through relevant signaling pathways. Previously, was thought be detrimental body, but further research shown appropriate promotes regeneration, formation. On contrary, excessive cause tissue damage pathological changes, even lead neurological diseases. Therefore, after various interact with cytokines chemokines promote repair by inhibiting negative harnessing positive specific ways at times. Understanding interaction between neuroinflammation provides several therapeutic ideas improve

Language: Английский

Citations

11
Stem Cell and Regenerative Therapies for the Treatment of Osteoporotic Vertebral Compression Fractures DOI Open Access
Songzi Zhang, Yunhwan Lee, Yanting Liu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4979 - 4979

Published: May 2, 2024

Osteoporotic vertebral compression fractures (OVCFs) significantly increase morbidity and mortality, presenting a formidable challenge in healthcare. Traditional interventions such as vertebroplasty kyphoplasty, despite their widespread use, are limited addressing the secondary effects of adjacent areas do not facilitate bone regeneration. This review paper explores emerging domain regenerative therapies, spotlighting stem cell therapy’s transformative potential OVCF treatment. It thoroughly describes therapeutic possibilities mechanisms action mesenchymal cells against OVCFs, relying on recent clinical trials preclinical studies for efficacy assessment. Our findings reveal that therapy, particularly combination with scaffolding materials, holds substantial promise regeneration, spinal stability improvement, pain mitigation. integration cell-based methods conventional treatments may herald new era management, potentially improving patient outcomes. advocates accelerated research collaborative efforts to translate laboratory breakthroughs into practice, emphasizing revolutionary impact therapies management. In summary, this positions therapy at forefront innovation treatment, stressing importance ongoing cross-disciplinary collaboration unlock its full potential.

Language: Английский

Citations

7
Efficiently directing differentiation and homing of mesenchymal stem cells to boost cartilage repair in osteoarthritis via a nanoparticle and peptide dual-engineering strategy DOI
Cuixi Wu,

Zhenwen Huang,

Jianmao Chen

et al.

Biomaterials, Journal Year: 2024, Volume and Issue: 312, P. 122720 - 122720

Published: July 27, 2024

Language: Английский

Citations

7
YAP1 mediates the dimensional and chemical coordination of immunoregulation and therapy in extensively passaged mesenchymal stem cells DOI Creative Commons

Fangying Du,

Feng Zhou, Na Zhao

et al.

Theranostics, Journal Year: 2025, Volume and Issue: 15(5), P. 1930 - 1948

Published: Jan. 6, 2025

Rationale: Mesenchymal stem cells (MSCs) possess potent immunomodulatory capability, but occasionally, clinical application of MSCs is hindered by compromised cell functionality and insufficient therapeutic efficacy. Methods: Here, well-established mouse models dextran sulfate sodium (DSS)-induced colitis streptozotocin (STZ)-induced type 1 diabetes (T1D) were used to evaluate effects human umbilical cord-derived MSCs. examined at the fifth (P5) fifteenth (P15) passages, three-dimensional (3D) culture was conducted Matrigel incorporation. A series biochemical, histopathological cellular assays performed investigate MSC function performance, immunoregulation evaluated in vitro co-culture with T vivo analyses T-cell infiltration into target tissues. RNA sequencing (RNA-seq) analysis followed immunofluorescence staining, gene expression chemical regulation molecular targets. Results: lose after extensive expansion P15 when senescence occurs. Intriguingly, 3D preconditioning promotes diminished immunoregulatory capability despite which benefits P15-MSCs modulate subsets co-culture, suppress pro-inflammatory colon pancreas tissues infusion, ameliorate systemic inflammation, alleviate T1D mice. Mechanistically, provokes transcriptomic reprogramming toward a Yes-associated protein (YAP1)-marked, Hippo signaling pathway-upregulated state promoted release anti-inflammatory cytokine, transforming growth factor-beta1 (TGF-β1). Moreover, YAP1 clinically relevant drugs, verteporfin (VP) prostaglandin E2 (PGE2), affects TGF-β1 during dimensional culture. Conclusions: Taken together, these findings unravel YAP1-based coordination expanded immunoregulation, will shed light on precisely controlled translational application.

Language: Английский

Citations

0
Stem cell approaches for tendon repair: delusion or insight DOI
Saswati Kar, Enhao Zhang,

Angel Moonilall

et al.

The Journal of Physiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

Abstract Tendon injuries are a common and significant health burden around the world. Current treatment strategies unable to fully restore tendon structure function. To address these critical clinical needs, stem cell therapy has recently emerged as promising approach for better repair. In this review, we discuss efficacy of in repair by summarizing its advantages disadvantages both preclinical settings. We highlight recent advances improving cell‐based treatment, with focus on research efforts identification appropriate sources, modification effective transplantation, development carriers, standardization protocols. Finally, provide insights into future directions therapy, emphasis necessity employing patient screening criteria before establishing comprehensive outcome measurements ensure rigor trials. image

Language: Английский

Citations

0
Emerging Landscape of Mesenchymal Stem Cell Senescence Mechanisms and Implications on Therapeutic Strategies DOI
Jing Wang, Muqing Zhang, Hu Wang

et al.

ACS Pharmacology & Translational Science, Journal Year: 2024, Volume and Issue: 7(8), P. 2306 - 2325

Published: July 17, 2024

Mesenchymal stem cells (MSCs) hold significant promise for regenerative medicine and tissue engineering due to their unique multipotent differentiation ability immunomodulatory properties. MSC therapy is widely discussed utilized in clinical treatment. However, during both vitro expansion vivo transplantation, MSCs are prone senescence, an irreversible growth arrest characterized by morphological, gene expression, functional changes genomic regulation. The microenvironment surrounding plays a crucial role modulating senescence phenotype, influenced factors such as hypoxia, inflammation, aging status. Numerous strategies targeting have been developed, including senolytics senomorphic agents, antioxidant exosome therapies, mitochondrial transfer, niche modulation. Novel approaches addressing replicative also emerged. This paper comprehensively reviews the current molecular manifestations of addresses environmental impact on highlights potential therapeutic mitigate MSC-based therapies. These insights aim enhance efficacy understanding

Language: Английский

Citations

3
Research progress of autoimmune diseases based on induced pluripotent stem cells DOI Creative Commons

Rutong Ren,

Jinhe Jiang,

Xinxin Li

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: April 24, 2024

Autoimmune diseases can damage specific or multiple organs and tissues, influence the quality of life, even cause disability death. A 'disease in a dish' be developed based on patients-derived induced pluripotent stem cells (iPSCs) iPSCs-derived disease-relevant cell types to provide platform for pathogenesis research, phenotypical assays, therapy, drug discovery. With rapid progress molecular biology research methods including genome-sequencing technology, epigenetic analysis, '-omics' analysis organoid large amount data represents an opportunity help gaining in-depth understanding pathological mechanisms developing novel therapeutic strategies these diseases. This paper aimed review iPSCs-based phenotype confirmation, mechanism exploration, discovery, therapy autoimmune diseases, especially sclerosis, inflammatory bowel disease, type 1 diabetes using iPSCs cells.

Language: Английский

Citations

1
Interplay Between Skeletal and Hematopoietic Cells in the Bone Marrow Microenvironment in Homeostasis and Aging DOI
Emily R. Quarato, Noah Salama, Laura M. Calvi

et al.

Current Osteoporosis Reports, Journal Year: 2024, Volume and Issue: 22(4), P. 416 - 432

Published: May 23, 2024

Language: Английский

Citations

0
Lysine-specific methyltransferase Set7/9 in stemness, differentiation, and development DOI Creative Commons
Alexandra Daks,

Sergey Parfenyev,

Oleg Shuvalov

et al.

Biology Direct, Journal Year: 2024, Volume and Issue: 19(1)

Published: May 29, 2024

Abstract The enzymes performing protein post-translational modifications (PTMs) form a critical regulatory circuitry that orchestrates literally all cellular processes in the organism. In particular, balance between stemness and differentiation is crucial for development of multicellular organisms. Importantly, fine-tuning this on genetic level largely mediated by specific PTMs histones including lysine methylation. Lysine methylation carried out special (lysine methyltransferases) transfer methyl group from S-adenosyl-L-methionine to residues substrates. Set7/9 one exemplary methyltransferases however, has not been fully studied yet. It was originally discovered as histone H3 4-specific methyltransferase, which later shown methylate number non-histone proteins are regulators differentiation, p53, pRb, YAP, DNMT1, SOX2, FOXO3, others. review we summarize information available date role tissue during embryogenesis adult Finally, highlight discuss pathological associated with aberrant self-renewal, formation cancer stem cells.

Language: Английский

Citations

0
3D Culture of MSCs for Clinical Application DOI Creative Commons
Qi Gao, Mehmet Sertac Cekuc, Yasemin Sude Ergul

et al.

Bioengineering, Journal Year: 2024, Volume and Issue: 11(12), P. 1199 - 1199

Published: Nov. 27, 2024

Mesenchymal stem cells (MSCs) play an important role in regenerative medicine and drug discovery due to their multipotential differentiation capabilities immunomodulatory effects. Compared with traditional 2D cultures of MSCs, 3D MSCs have emerged as effective approach enhance cell viability, proliferation, functionality, provide a more relevant physiological environment. Here, we review the therapeutic potential 3D-cultured highlighting roles tissue regeneration repair screening. We further summarize successful cases that apply modeling disease states, enabling identification novel strategies. Despite these promising applications, discuss challenges remain clinical translation MSC technologies, including stability, heterogeneity, regulatory issues. conclude by addressing obstacles emphasizing need for research fully exploit practice.

Language: Английский

Citations

0