Marine Drugs,
Journal Year:
2025,
Volume and Issue:
23(3), P. 124 - 124
Published: March 13, 2025
In
this
study,
we
identified
AlgVR7,
a
novel
bifunctional
alginate
lyase
from
Vibrio
rumoiensis
and
characterized
its
biochemical
properties
substrate
specificity.
Sequence
alignment
analysis
inferred
the
key
residues
K267,
H162,
N86,
E189,
T244
for
AlgVR7
catalysis,
it
is
derived
PL7
family;
exhibited
high
activity
towards
sodium
alginate,
polyM
(PM),
polyG
(PG);
can
also
degrade
polygalacturonic
acid
(PGA)
efficiently,
with
highest
affinity
catalytic
efficiency
MG
block
of
substrate.
The
optimal
temperature
pH
were
determined
to
be
40
°C
8,
respectively.
enzyme
was
maximum
at
°C,
40%
retained
after
incubation
60
min,
still
present
min
incubation.
stimulated
by
100
Mm
NaCl,
indicating
halophilic
nature
suitability
marine
environments.
Degradation
products
analyzed
using
ESI-MS
revealed
that
primarily
produced
trisaccharides
tetrasaccharides.
At
8.0,
Km
values
PM,
PG
16.67
μmol,
13.12
22.86
Structural
molecular
docking
studies
unveiled
involved
in
recognition
interaction.
Glu167
as
critical
residue
PL7_5
subfamily,
uniquely
playing
an
essential
role
decomposition.
Overall,
exhibits
great
potential
powerful
efficient
preparation
oligosaccharides,
promising
applications
biotechnology
industrial
fields.
Metabolic Engineering,
Journal Year:
2022,
Volume and Issue:
71, P. 42 - 61
Published: Jan. 22, 2022
Marine
macroalgae
have
huge
potential
as
feedstocks
for
production
of
a
wide
spectrum
chemicals
used
in
biofuels,
biomaterials,
and
bioactive
compounds.
Harnessing
these
ways
could
promote
wellbeing
people
while
mitigating
climate
change
environmental
destruction
linked
to
use
fossil
fuels.
Microorganisms
play
pivotal
roles
converting
into
valuable
products,
metabolic
engineering
technologies
been
developed
extend
their
native
capabilities.
This
review
showcases
current
achievements
the
metabolisms
various
microbial
chassis
convert
red,
green,
brown
bioproducts.
Unique
features
macroalgae,
such
seasonal
variation
carbohydrate
content
salinity,
provide
next
challenges
advancing
macroalgae-based
biorefineries.
Three
emerging
strategies
are
discussed
here:
(1)
designing
dynamic
control
pathways,
(2)
strains
halophilic
(salt-tolerant)
microbes,
(3)
developing
consortia
conversion.
illuminates
opportunities
future
research
communities
by
elucidating
approaches
microbes
so
they
can
become
cell
factories
utilization
feedstocks.
Molecules,
Journal Year:
2022,
Volume and Issue:
27(11), P. 3375 - 3375
Published: May 24, 2022
The
cell
wall
of
brown
algae
contains
alginate
as
a
major
constituent.
This
anionic
polymer
is
composite
β-d-mannuronate
(M)
and
α-l-guluronate
(G).
Alginate
can
be
degraded
into
oligosaccharides;
both
the
its
products
exhibit
antioxidative,
antimicrobial,
immunomodulatory
activities
and,
hence,
find
many
commercial
applications.
attacked
by
various
enzymes,
collectively
termed
lyases,
that
degrade
glycosidic
bonds
through
β-elimination.
Considering
abundance
in
marine
ecosystems,
an
important
source
nutrients
for
organisms,
therefore,
lyases
play
significant
role
carbon
recycling.
Various
microorganisms,
particularly
those
thrive
association
with
algae,
have
been
reported
producers
lyases.
Conceivably,
marine-derived
demonstrate
salt
tolerance,
are
activated
presence
salts
applications
food
industry.
Therefore,
this
review
summarizes
structural
biochemical
features
bacterial
along
their
comprehensive
information
aid
expansion
future
prospects
Fermentation,
Journal Year:
2023,
Volume and Issue:
9(5), P. 463 - 463
Published: May 12, 2023
The
cosmetic
segment
is
a
rapidly
growing
industry
that
has
been
challenged
in
recent
years
due
to
the
origin
and
impact
of
its
ingredients
manufacturing
techniques.
With
focus
on
reducing
carbon
dioxide
emissions
improving
degradability
products,
many
conventional
are
being
dismissed
meet
more
exigent
regulations
consumer
ethical
demands.
Biotechnology,
fermentation
as
core
technology,
solution
support
drive
sustainable
growth
for
industry.
This
review
presents
latest
research
development
applied
cosmetics
showcases
multiple
examples
throughout
all
classes
ingredients:
from
functional
compounds,
such
oil
surfactants,
multi-faceted
molecules
with
wide
spectrum
formulations
skin
benefits
derived
their
emulsifying,
antimicrobial
or
antioxidant
properties.
bottlenecks
associated
commercialization
ingredients,
together
successful
examples,
also
discussed.
shift
towards
bio-based
beauty
requires
combination
technical,
regulatory
marketing
efforts.
Fermentation
strategies
better
utilize
low-cost
substrates
optimize
microorganisms
processes
will
reduce
overall
costs,
price
gap
traditional
methods
production.
testing,
standardization
regulation
these
new
need
catch
up
fast
happening
field.
Finally,
communication
key
achieve
introduction
biotech
market.
Applied and Environmental Microbiology,
Journal Year:
2024,
Volume and Issue:
90(2)
Published: Jan. 23, 2024
Marine
bacteria
play
important
roles
in
the
degradation
and
cycling
of
algal
polysaccharides.
However,
dynamics
epiphytic
bacterial
communities
their
polysaccharide
during
kelp
decay
are
still
unclear.
Here,
we
performed
metagenomic
analyses
to
investigate
identities
predicted
metabolic
abilities
early
late
stages
PLoS Biology,
Journal Year:
2025,
Volume and Issue:
23(4), P. e3003038 - e3003038
Published: April 1, 2025
A
major
goal
of
evolutionary
biology
is
to
identify
the
genetic
basis
for
emergence
complex
adaptive
traits.
Diatoms
are
ancestrally
photosynthetic
microalgae.
However,
in
genus
Nitzschia
,
loss
photosynthesis
led
a
group
free-living
secondary
heterotrophs
whose
manner
acquiring
chemical
energy
unclear.
Here,
we
sequence
genome
non-photosynthetic
diatom
sing1
and
its
catabolism
brown
algal
cell
wall
polysaccharide
alginate.
N
.
obtained
an
endolytic
alginate
lyase
enzyme
by
horizontal
gene
transfer
(HGT)
from
marine
bacterium.
Subsequent
duplication
through
unequal
crossing
over
transposition
91
genes
three
distinct
families.
One
family
retains
ancestral
function.
By
contrast,
two
others
underwent
domain
duplication,
gain,
loss,
rearrangement,
mutation
encode
novel
functions
that
can
account
oligosaccharide
import
endomembrane
system
exolytic
production
monosaccharides.
Together,
our
results
show
how
single
HGT
event
followed
substantial
neofunctionalization
access
new
ecological
niche.
