Multi-Functional Alginate Lyase AlgVR7 from Vibrio rumoiensis: Structural Insights and Catalytic Mechanisms DOI Creative Commons
Zhe Huang, Shuai Liang, Wenhui Jiang

et al.

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(3), P. 124 - 124

Published: March 13, 2025

In this study, we identified AlgVR7, a novel bifunctional alginate lyase from Vibrio rumoiensis and characterized its biochemical properties substrate specificity. Sequence alignment analysis inferred the key residues K267, H162, N86, E189, T244 for AlgVR7 catalysis, it is derived PL7 family; exhibited high activity towards sodium alginate, polyM (PM), polyG (PG); can also degrade polygalacturonic acid (PGA) efficiently, with highest affinity catalytic efficiency MG block of substrate. The optimal temperature pH were determined to be 40 °C 8, respectively. enzyme was maximum at °C, 40% retained after incubation 60 min, still present min incubation. stimulated by 100 Mm NaCl, indicating halophilic nature suitability marine environments. Degradation products analyzed using ESI-MS revealed that primarily produced trisaccharides tetrasaccharides. At 8.0, Km values PM, PG 16.67 μmol, 13.12 22.86 Structural molecular docking studies unveiled involved in recognition interaction. Glu167 as critical residue PL7_5 subfamily, uniquely playing an essential role decomposition. Overall, exhibits great potential powerful efficient preparation oligosaccharides, promising applications biotechnology industrial fields.

Language: Английский

Modified polysaccharides for food packaging applications: A review DOI
Weronika Janik, Łukasz Jakubski, S. Kudła

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 258, P. 128916 - 128916

Published: Dec. 20, 2023

Language: Английский

Citations

19
Recent progress in the efficacy of algal saccharides on skin repair DOI

Yuansen Liu,

Yi Zhu,

Quanxing Zheng

et al.

Algal Research, Journal Year: 2024, Volume and Issue: 78, P. 103403 - 103403

Published: Jan. 26, 2024

Language: Английский

Citations

5
Hybrid polycomplexes of anionic alginate with a synthetic cationic polymer: attractive and poisonous for microorganisms DOI Open Access

Darya G. Sinelnikova,

Оlga А. Novoskoltseva, Н. Г. Лойко

et al.

Mendeleev Communications, Journal Year: 2024, Volume and Issue: 34(1), P. 31 - 33

Published: Jan. 1, 2024

Language: Английский

Citations

5
Efficient preparation of alginate oligosaccharides by using alginate lyases and evaluation of the development promoting effects on Brassica napus L. in saline-alkali environment DOI
Benwei Zhu, Li Li, Xinyu Yuan

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 270, P. 131917 - 131917

Published: April 26, 2024

Language: Английский

Citations

5
Author response: Polysaccharide breakdown products drive degradation-dispersal cycles of foraging bacteria through changes in metabolism and motility DOI Open Access
Astrid KM Stubbusch, Johannes M. Keegstra, Julia Schwartzman

et al.

Published: July 24, 2024

Most of Earth's biomass is composed polysaccharides. During decomposition, polysaccharides are degraded by heterotrophic bacteria as a nutrient and energy source thereby partly remineralized into CO2. As heterogeneously distributed in nature, following the colonization degradation polysaccharide hotspot cells need to reach new hotspots. Even though many studies indicate that these degradation-dispersal cycles contribute carbon flow marine systems, we know little about how alternate between motility, which environmental factors trigger this behavioral switch. Here, studied growth bacterium Vibrio cyclitrophicus ZF270 on abundant alginate, both its soluble polymeric form well breakdown products. We used microfluidics coupled time-lapse microscopy analyze motility individual cells, RNA sequencing study associated changes gene expression. found single grow at reduced rate alginate until they large groups cooperatively break down polymer. Exposing cell digested accelerates expression genes involved catabolism, central metabolism, ribosomal biosynthesis, transport. However, exposure also triggers become motile disperse from groups, proportionally increasing with group size before switch, accompanied high flagellar assembly, chemotaxis, quorum sensing. chemotax toward but not likely enabling them find Overall, our findings reveal cellular mechanisms might underlie bacterial cycles, influence remineralization environments.Polysaccharides, known glycans, most Earth understanding microorganisms essential for global cycle storage release CO2 natural systems. Although formation common strategy degrade ubiquitous substrates where hotspots distributed, prepare dispersal an exhausted re-initiate patches. By quantifying growth, chemotaxis comparing when populations were exposed either or their products polysaccharides, show alter behavior experience shift polysaccharides: After during polymers, triggered motile, sessile groups. The chemotactic attraction allows move towards Our sheds light processes drive remineralization, important process resulting

Language: Английский

Citations

5
Advances of conductive hydrogel designed for flexible electronics: A review DOI
Guanzhou Zhu,

Negar Javanmardia,

Lili Qian

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 136115 - 136115

Published: Sept. 1, 2024

Language: Английский

Citations

5
Discerning Poly- and Monosaccharide Enrichment Mechanisms: Alginate and Glucuronate Adsorption to a Stearic Acid Sea Surface Microlayer DOI

Maria G. Vazquez de Vasquez,

Mickey M. Rogers, Kimberly A. Carter-Fenk

et al.

ACS Earth and Space Chemistry, Journal Year: 2022, Volume and Issue: 6(6), P. 1581 - 1595

Published: May 20, 2022

Poly- and monosaccharides are ubiquitous in the marine environment enriched sea spray aerosol but mechanisms for their enrichment not fully understood. We expand upon previously defined co-adsorption by investigating co-absorption of alginate its representative monomeric form, glucuronate, to a stearic acid monolayer as function saccharide concentration on an ocean proxy solution. Using Langmuir isotherms, surface-sensitive infrared reflection–absorption spectroscopy, Brewster angle microscopy, we demonstrate that mechanism significantly differs between glucuronate. find film thickness increases from ∼3.5 4.8 nm ∼3.7 glucuronate stearic-d35 monolayers, respectively, indicating forms multilayers underneath monolayer. Glucuronate shows different where it likely intercalates induces significant reorganization within quantify adsorption coefficients half-saturation concentrations both acid. produces constants Kalginate = 0.089 ± 0.015 C1/2,alginate 11 1.9 mg/L Kglucuronate 0.081 C1/2,glucuronate 12 2.3 Thus, use alone is insufficient, provide important parameters better representation saccharides investigations.

Language: Английский

Citations

19
Gut bacterial alginate degrading enzymes DOI
Mette E. Rønne, Mikkel Madsen, Tobias Tandrup

et al.

Essays in Biochemistry, Journal Year: 2023, Volume and Issue: 67(3), P. 387 - 398

Published: April 1, 2023

Alginates are abundant marine anionic polysaccharides consumed by humans. Thus, over the years some understanding has emerged about alginate utilization human gut microbiota (HGM). However, insights have been obtained only recently at molecular level with regard to structure and function of degrading metabolizing enzymes from HGM. Still, numerous studies report on effects alginates bacterial communities digestive tracts various, predominantly organisms feeding involved lyases characterized. Other describe beneficial impact elicited in animal models, for example, high-fat-diet-fed mice addressing obesity or as feed supplements livestock. depolymerized a β-elimination reaction catalyzed polysaccharide (PLs) referred (ALs). The ALs found 15 42 PL families categorized CAZy database. While genome mining led prediction encoded bacteria HGM; currently, four this niche characterized biochemically two crystal structures reported. composed mannuronate (M) guluronate (G) residues organized M-, G-, MG-blocks, which calls complementary specificity effectively depolymerize oligosaccharides (AOSs) monosaccharides. Typically, different genes arranged clusters denoted loci. Currently, biochemical structural analyses contribute depicting mode action predicted

Language: Английский

Citations

12
The Enzymatic Process of Macroalgae for Conversion into High-tech Bioproducts DOI Open Access

JooHee Han,

YeWon Jo,

Habin Sun

et al.

Biotechnology and Bioprocess Engineering, Journal Year: 2023, Volume and Issue: 28(3), P. 356 - 370

Published: June 1, 2023

Language: Английский

Citations

12
Efficient Degradation of Alginate and Preparation of Alginate Oligosaccharides by a Novel Biofunctional Alginate Lyase with High Activity and Excellent Thermophilic Features DOI Creative Commons
Li Li,

Shengsheng Cao,

Benwei Zhu

et al.

Marine Drugs, Journal Year: 2023, Volume and Issue: 21(3), P. 180 - 180

Published: March 14, 2023

The enzymatic degradation of seaweed polysaccharides is gaining interest for its potential in the production functional oligosaccharides and fermentable sugars. Herein, a novel alginate lyase, AlyRm3, was cloned from marine strain, Rhodothermus marinus DSM 4252. AlyRm3 showed optimal activity (37,315.08 U/mg) at 70 °C pH 8.0, with sodium used as substrate. Noticeably, stable 65 also exhibited 30% maximal 90 °C. These results indicated that thermophilic lyase efficiently degrades high industrial temperatures (>60 °C). FPLC ESI-MS analyses suggested primarily released disaccharides trisaccharides alginate, polyM, polyG an endolytic manner. In saccharification process (0.5%, w/v), yielded numerous reducing sugars (1.73 g/L) after 2 h reaction. has capacity saccharifying could be to saccharify biomass before main fermentation biofuels. properties make valuable candidate both fundamental research applications.

Language: Английский

Citations

11