Adaptive laboratory evolution of Thermoanaerobacterium aotearoense for enhanced ethanol production from raw cassava starch DOI

Kaiqun Dai,

Yuanhao Miao,

Chunyun Qu

et al.

Renewable Energy, Journal Year: 2024, Volume and Issue: 233, P. 121179 - 121179

Published: Aug. 14, 2024

Language: Английский

Hi-TARGET: a fast, efficient and versatile CRISPR type I-B genome editing tool for the thermophilic acetogen Thermoanaerobacter kivui DOI Creative Commons

Angeliki Sitara,

Rémi Hocq, Alexander Lu

et al.

Biotechnology for Biofuels and Bioproducts, Journal Year: 2025, Volume and Issue: 18(1)

Published: April 30, 2025

Language: Английский

Citations

1
Cofactor engineering in Thermoanaerobacterium aotearoense SCUT27 for maximizing ethanol yield and revealing an enzyme complex with high ferredoxin-NAD+ reductase activity DOI

Kaiqun Dai,

Chunyun Qu, Xin Li

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 402, P. 130784 - 130784

Published: May 1, 2024

Language: Английский

Citations

4
Hi-TARGET: A fast, efficient and versatile CRISPR type I-B genome editing tool for the thermophilic acetogen Thermoanaerobacter kivui DOI Creative Commons

Angeliki Sitara,

Rémi Hocq, Alexander Lu

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Abstract Background Due to its ability grow fast on CO2, CO and H2 at high temperatures with energy efficiency, the thermophilic acetogen Thermoanaerobacter kivui could become an attractive host for industrial biotechnology. In a circular carbon economy, diversification upgrading of C1 platform feedstocks into value-added products (e. g. ethanol, acetone isopropanol) crucial. To that end, genetic bioprocess engineering tools are required facilitate development bioproduction scenarios. Currently, genome editing available T. present some limitations in speed thus restricting powerful strain chassis applications. Results this study, we developed versatile tool Hi-TARGET, based endogenous CRISPR Type I-B system kivui. Hi-TARGET demonstrated 100% efficiency gene knock-out (from both purified plasmid cloning mixture) knock-in, 49% creating point mutations. Furthermore, optimized transformation plating protocol increased by 245-fold 1.96 x 104 ± 8.7 103 CFU µg− 1. Subsequently, was used demonstrate knock-outs (pyrE, rexA, hrcA), knock-in (ldh::pFAST), single nucleotide mutation corresponding PolCC629Y, knock-down fluorescent protein pFAST. Analysis ∆rexA deletion mutant created revealed transcriptional repressor rexA is likely involved regulation expression lactate dehydrogenase (ldh). Following engineering, curing procedure edited strains devised. total, time from DNA clean, 12 days, rendering fast, robust complete method Conclusions The CRISPR-based can be scarless deletion, insertion, assays, fast-tracking generation industrially-relevant production carbon-negative chemicals fuels as well facilitating studies metabolism physiology.

Language: Английский

Citations

0
High-Temperature Catalytic Platform Powered by Thermophilic Microorganisms and Thermozymes DOI
Jiawei Li, Lichao Sun, Yi‐Xin Huo

et al.

Synthetic biology and engineering, Journal Year: 2025, Volume and Issue: 3(1), P. 10001 - 10001

Published: Jan. 1, 2025

Language: Английский

Citations

0
Designing Thermophilic, Synthetic Microbial Communities for Consolidated Bioprocessing DOI Creative Commons
Hansen Tjo,

Kelly Blundin,

Jonathan M. Conway

et al.

BioDesign Research, Journal Year: 2025, Volume and Issue: unknown, P. 100024 - 100024

Published: April 1, 2025

Language: Английский

Citations

0
Multidimensional engineering and peptidoglycan modulation for surfactin production in Bacillus subtilis DOI
Xia Li,

Zheng-Jie Hou,

Fuzhou Zhu

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 163954 - 163954

Published: May 1, 2025

Language: Английский

Citations

0
Sugar transport in thermophiles: Bridging lignocellulose deconstruction and bioconversion DOI Creative Commons
Hansen Tjo, Jonathan M. Conway

Journal of Industrial Microbiology & Biotechnology, Journal Year: 2024, Volume and Issue: 51

Published: Jan. 1, 2024

Biomass degrading thermophiles play an indispensable role in building lignocellulose-based supply chains. They operate at high temperatures to improve process efficiencies and minimize mesophilic contamination, can overcome lignocellulose recalcitrance through their native carbohydrate-active enzyme (CAZyme) inventory, utilize a wide range of sugar substrates. However, transport is poorly understood investigated, as compared enzymatic deconstruction metabolic conversion sugars value-added chemicals. Here, we review the general modes thermophilic bacteria archaea, covering structural, molecular, biophysical basis high-affinity uptake. We also discuss recent genetic studies on transporter function. With this understanding transport, strategies for how be engineered thermophiles, with potential enhance lignocellulosic biomass into renewable products.

Language: Английский

Citations

3
A Short Review of Second-Generation Isobutanol Production by SHF and SSF DOI Creative Commons
Hironaga Akita, Akinori Matsushika

Applied Biosciences, Journal Year: 2024, Volume and Issue: 3(3), P. 296 - 309

Published: July 8, 2024

As isobutanol exhibits higher energy density and lower hygroscopicity than ethanol, it is considered a better candidate biofuel. The sustainable supply of inedible biomass lack competition with the food have stimulated significant worldwide interest in production from this resource. Both separate hydrolysis fermentation (SHF) simultaneous saccharification (SSF) been applied to effectively utilize as feedstock. However, both processes various challenges, including low yield high costs. This review summarizes potential biofuel, methods for conferring productivity, recent experimental studies, developments SHF SSF isobutanol-producing strains. Challenges increasing suggestions improvements enable commercial are also discussed.

Language: Английский

Citations

0
Adaptive laboratory evolution of Thermoanaerobacterium aotearoense for enhanced ethanol production from raw cassava starch DOI

Kaiqun Dai,

Yuanhao Miao,

Chunyun Qu

et al.

Renewable Energy, Journal Year: 2024, Volume and Issue: 233, P. 121179 - 121179

Published: Aug. 14, 2024

Language: Английский

Citations

0