BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)
Published: Oct. 4, 2024
Language: Английский
Science Advances, Journal Year: 2025, Volume and Issue: 11(5)
Published: Jan. 31, 2025
The hypervirulent carbapenem-resistant Klebsiella pneumoniae (hv-CRKP) poses a substantial challenge to the global health care. However, mechanism behind its evolution and transmission remain elusive. Here, four virulence plasmid types were identified from 310 hv-CRKP isolates collected nationwide during 2017–2018, based on their aerobactin ( iuc locus) lineage IncFIB replicons. Notably, pIUC1-IncFIB(K) 37 pIUC1-IncFIB(Mar), representing two epidemic plasmids in Asia Europe, respectively, accounted for >90% of episodes. Analysis 494 K. (376 2010–2013; 118 2017–2018) 2578 public genomes indicated notable role IncFIB(Mar) emergence spread. Conjugation assays showed helper could efficiently transfer into strain uniquely retromobilize with back CRKP. Thereafter, either lost rapidly or recombined , generating hybrid pIUC1-IncFIB(Mar) plasmid. Our findings elucidated formation, evolution, dissemination trajectories major strains different regions.
Language: Английский
Citations
2
Genome Medicine, Journal Year: 2023, Volume and Issue: 15(1)
Published: Feb. 13, 2023
Klebsiella pneumoniae, which is frequently associated with hospital- and community-acquired infections, contains multidrug-resistant (MDR), hypervirulent (hv), non-MDR/non-hv as well convergent representatives. It known that mostly international high-risk clonal lineages including sequence types (ST) 11, 147, 258, 307 drive their global spread. ST395, was first reported in the context of a carbapenemase-associated outbreak France 2010, less well-characterized, yet emerging lineage.
Language: Английский
Citations
37
International Journal of Medical Microbiology, Journal Year: 2024, Volume and Issue: 314, P. 151601 - 151601
Published: Feb. 8, 2024
Klebsiella (K.) pneumoniae is a ubiquitous Gram-negative bacterium and common coloniser of animals humans. Today, K. one the most persistent nosocomial pathogens worldwide poses severe threat/burden to public health by causing urinary tract infections, pneumonia bloodstream infections. Affected infections are mainly immunocompromised individuals hospitalised patients. In recent years, new type has emerged associated with community-acquired such as pyogenic liver abscess in otherwise healthy therefore termed hypervirulent (hvKp). The aim this study was characterisation isolates properties hypervirulence from Germany. A set 62 potentially human patients compiled. Inclusion criteria were presence at least determinant that been previously hypervirulence: (I) clinical manifestation, (II) positive string test marker for hypermucoviscosity, (III) virulence genes rmpA and/or rmpA2 magA. Phenotypic included antimicrobial resistance testing broth microdilution. Whole genome sequencing (WGS) performed using Illumina® MiSeq, NextSeq investigate genetic repertoire multi-locus sequence types (ST), capsule (K), further collected isolates. For selected long-read applied plasmid sequences determinants compared. WGS analyses confirmed several signature hvKp. Among them, prevalent siderophore loci iuc ybt regulator rmpA2. dominant ST among hvKp ST395 K2 K5; both have described our data multidrug-resistant (MDR) ST23 K1 second abundant study; commonly hypervirulence. general, beta-lactams caused production extended-spectrum beta-lactamases (ESBL) carbapenemases observed frequently isolates, confirming threatening rise MDR-hvKp strains. Our results show strains carry present many years detection carbapenemase on same highly problematic requires intensified screening molecular surveillance. However, non-uniform definition complicates their detection. Testing hypermucoviscosity alone not specific enough identify Thus, we suggest classification should be only fulfil phenotypical (severe manifestations, hypermucoviscosity) but also two e.g. ybt,
Language: Английский
Citations
10
Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 89 - 89
Published: Jan. 5, 2025
Klebsiella pneumoniae represent a common invasive infection etiological agent, whose potential carbapenem-resistance and hypermucoviscosity complicate the patient's management. Infection development often derives from gastrointestinal colonization; thus, it is fundamental to monitor asymptomatic K. colonization through surveillance protocols, especially for intensive care immunocompromised patients. We described six-month routine screening protocol Policlinico of Catania (Italy), while blood samples were collected same patients only in cases systemic suspicion. All who had dissemination episodes furtherly investigated next-generation sequencing, analyzing both colonizing disseminating strains. This study documents emerging sequence types such as ST101, ST307, ST395, mainly revealing blaNDM or blaKPC genes, along with siderophores hyperproduction capsule markers virulence factors. Most detected factors are presumably related specific plasmid content, which extremely varied rich. In conclusion, active sequencing essential enhance awareness local epidemiology within high-risk multi-drug resistance areas. A random analysis on most warning microorganisms could typing (ST) settings, allowing better prevention control strategies their eventual persistence diffusion.
Language: Английский
Citations
1
Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)
Published: June 26, 2023
Infections caused by multi-drug resistant Enterobacterales (MDR-E) are difficult to treat and cause significant mortality, especially in developing countries. This study characterized the phenotypic genotypic profiles of 49 randomly selected beta-lactam MDR-E previously isolated from patients being managed hospitals Nigeria using whole genome sequencing. The isolates exhibited 85.5% resistance 3rd generation cephalosporins 65.3% carbapenems. blaTEM-1B (29, 59.2%), blaCTX-M-15 (38, 77.6%), blaNDM-1 (17, 51.5%) were most common penicillinase, ESBL, carbapenem genes across isolates, respectively. Seventeen (45%) was carried on insertion sequence ISEc9 while (11, 64.7%) associated with ISEc33. None 21 plasmids detected β-lactamase genes. Higher rates found E. coli ST-88 (n = 2) high-risk ST-692 2). For Klebsiella species, clones ST-476 8) ST-147 3) predominated had higher number AMR mechanisms pattern antibiotic differ patterns described harbouring a wide range AMRGs. detection several chromosomally mediated carbapenemases our also represents finding that warrants further investigation better understand its' implications for clinical practice public health. MDR-Es be pan-susceptible tigecycline very low fosfomycin, suggesting potential these as empiric treatments. A surveillance approach incorporating both conventional laboratory techniques modern molecular is essential comprehensive characterization emergence dissemination antimicrobial infections within Nigeria.
Language: Английский
Citations
15
Emerging infectious diseases, Journal Year: 2023, Volume and Issue: 29(8)
Published: July 10, 2023
Blood and surveillance cultures from an injured service member Ukraine grew Acinetobacter baumannii, Klebsiella pneumoniae, Enterococcus faecium, 3 distinct Pseudomonas aeruginosa strains. Isolates were nonsusceptible to most antibiotics carried array of antibiotic resistant genes, including carbapenemases (blaIMP-1, blaNDM-1, blaOXA-23, blaOXA-48, blaOXA-72) 16S methyltransferases (armA rmtB4).
Language: Английский
Citations
14
Drug Resistance Updates, Journal Year: 2024, Volume and Issue: 77, P. 101124 - 101124
Published: Aug. 2, 2024
Language: Английский
Citations
5
Microbiological Research, Journal Year: 2023, Volume and Issue: 275, P. 127446 - 127446
Published: July 6, 2023
Language: Английский
Citations
12
Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15
Published: Feb. 23, 2024
Objectives Carbapenem-resistance is a challenging healthcare concern and require specific stewardship programs. Monitoring workflows include the identification from surveillance samples, such as rectal swabs. Although culture assays represent gold standard, data report significant effectiveness in detecting carbapenemases genes directly The aim of this study was to evaluate REALQUALITY Carba-Screen kit (AB ANALITICA, Padova, Italy) swabs, also comparing its results. A next-generation sequencing (NGS) performed investigate positive samples about resistance markers sequence type (ST). Methods number 136 swabs were collected University Hospital Policlinico Catania critical wards. simultaneously underwent molecular (REALQUALITY kit). method included two-steps. first step (1 h 6 min) rapidly excluded negative while second one only for confirmation. All NGS analysis. Results Statistical evaluations demonstrated high sensitivity (100%) detection rates (92.6%) kit, which mostly correlated standard workflow. results matched results, mainly confirmed by resistome identified ST appeared be diversified different clinically significative strains same setting, furnishing interesting epidemiological evidence. Conclusion allowed coordinate approach high-prevalence multi-drug-resistance area. rapid with multi-step procedure accelerated infection control procedures, preliminary reduced overtreatment episodes. efficacy through NGS. In conclusion, screening could initially lead more conservative approach, may reevaluated after result microorganisms’ susceptibility profile.
Language: Английский
Citations
4
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: Sept. 27, 2024
Language: Английский
Citations
4