Functional Extracellular Vesicles for Regenerative Medicine DOI
Han Young Kim, Seunglee Kwon, Wooram Um

et al.

Small, Journal Year: 2022, Volume and Issue: 18(36)

Published: March 23, 2022

The unique biological characteristics and promising clinical potential of extracellular vesicles (EVs) have galvanized EV applications for regenerative medicine. Recognized as important mediators intercellular communication, naturally secreted EVs the potential, innate biotherapeutics, to promote tissue regeneration. Although emerged novel therapeutic agents, challenges related transition led further functionalization. In recent years, various engineering approaches such preconditioning, drug loading, surface modification been developed potentiate outcomes EVs. Also, limitations natural addressed by development artificial that offer advantages in terms production yield isolation methodologies. this review, an updated overview current techniques is provided functionalization advances EVs, particularly scope

Language: Английский

The power of imaging to understand extracellular vesicle biology in vivo DOI
Frederik J. Verweij, Leonora Balaj, Chantal M. Boulanger

et al.

Nature Methods, Journal Year: 2021, Volume and Issue: 18(9), P. 1013 - 1026

Published: Aug. 26, 2021

Language: Английский

Citations

266
The application of mesenchymal stromal cells (MSCs) and their derivative exosome in skin wound healing: a comprehensive review DOI Creative Commons

Donghui Bian,

Yan Wu, Guodong Song

et al.

Stem Cell Research & Therapy, Journal Year: 2022, Volume and Issue: 13(1)

Published: Jan. 24, 2022

Recently, mesenchymal stromal cells (MSCs) and also their exosome has become a game-changing tool in the context of tissue engineering regenerative medicine. MSCs due to competencies establish skin cells, such as fibroblast keratinocyte, unique attribute suppress inflammation wound site attracted increasing attention among scholars. In addition, MSC's other capabilities induce angiogenesis result secretion pro-angiogenic factors accompanied with marked anti-fibrotic activities, which mainly mediated by releases matrix metalloproteinase (MMPs), make them rational effective strategy accelerate healing small scar. Since chief properties depend on paracrine effects, it appears that MSCs-derived exosomes can be an alternative option support regeneration innovative cell-free approach. Such convey functional cargos (e.g., growth factor, cytokine, miRNA, etc.) from target thereby affecting recipient cells' biological events, migration, proliferation, ECM components collagen). The main superiorities therapy over parental are diminished risk tumor formation lower immunogenicity. Herein, we deliver overview recent vivo reports rendering therapeutic benefits MSCs-based therapies ease healing, so improving quality life patients suffering conditions.

Language: Английский

Citations

216
Exosomal miR-125b-5p deriving from mesenchymal stem cells promotes tubular repair by suppression of p53 in ischemic acute kidney injury DOI Creative Commons
Jing-Yuan Cao, Bin Wang, Tao‐Tao Tang

et al.

Theranostics, Journal Year: 2021, Volume and Issue: 11(11), P. 5248 - 5266

Published: Jan. 1, 2021

Mesenchymal stem cells-derived exosomes (MSC-exos) have attracted great interest as a cell-free therapy for acute kidney injury (AKI). However, the

Language: Английский

Citations

209
A paradigm shift in cell-free approach: the emerging role of MSCs-derived exosomes in regenerative medicine DOI Creative Commons

Soudeh Moghadasi,

Marischa Elveny, Heshu Sulaiman Rahman

et al.

Journal of Translational Medicine, Journal Year: 2021, Volume and Issue: 19(1)

Published: July 12, 2021

Abstract Recently, mesenchymal stem/stromal cells (MSCs) due to their pro-angiogenic, anti-apoptotic, and immunoregulatory competencies along with fewer ethical issues are presented as a rational strategy for regenerative medicine. Current reports have signified that the pleiotropic effects of MSCs not related differentiation potentials, but rather exerted through release soluble paracrine molecules. Being nano-sized, non-toxic, biocompatible, barely immunogenic, owning targeting capability organotropism, exosomes considered nanocarriers possible use in diagnosis therapy. Exosomes convey functional molecules such long non-coding RNAs (lncRNAs) micro-RNAs (miRNAs), proteins (e.g., chemokine cytokine), lipids from target cells. They participate intercellular interaction procedures enable repair damaged or diseased tissues organs. Findings evidenced alone liable beneficial influences myriad experimental models, suggesting MSC- can be utilized establish novel cell-free therapeutic treatment varied human disorders, encompassing myocardial infarction (MI), CNS-related musculoskeletal disorders (e.g. arthritis), kidney diseases, liver lung well cutaneous wounds. Importantly, compared MSCs, serve more steady entities reduced safety risks concerning injection live cells, microvasculature occlusion risk. In current review, we will discuss potential an innovative approach context medicine highlight recent knowledge on translational medicine, focusing vivo researches.

Language: Английский

Citations

178
Mesenchymal stem cells protect against ferroptosis via exosome-mediated stabilization of SLC7A11 in acute liver injury DOI Creative Commons
Feiyan Lin,

Wenyi Chen,

Jiahang Zhou

et al.

Cell Death and Disease, Journal Year: 2022, Volume and Issue: 13(3)

Published: March 26, 2022

Abstract Mesenchymal stem cells (MSCs) have attracted interest for their potential to alleviate liver injury. Here, the protective effect of MSCs on carbon tetrachloride (CCl 4 )-induced acute injury (ALI) was investigated. In this study, we illustrated a novel mechanism that ferroptosis, newly recognized form regulated cell death, contributed CCl -induced ALI. Subsequently, based in vitro and vivo evidence MSC-derived exosomes (MSC-Exo) treatment achieved pathological remission inhibited production lipid peroxidation, proposed an MSC-based therapy More intriguingly, with MSC-Exo downregulated mRNA level prostaglandin-endoperoxide synthase 2 ( Ptgs2 ) lipoxygenases LOXs while it restored protein SLC7A11 primary hepatocytes mouse liver, indicating inhibition ferroptosis partly accounted We further revealed MSC-Exo-induced expression accompanied by increasing CD44 OTUB1. The aberrant ubiquitinated triggered could be rescued OTUB1-mediated deubiquitination, thus strengthening stability thereby leading activation system X C − prevent hepatocyte ferroptosis. conclusion, showed had role against maintaining function, proposing therapeutic strategy ferroptosis-induced

Language: Английский

Citations

144
Three-Dimensional-Cultured MSC-Derived Exosome-Hydrogel Hybrid Microneedle Array Patch for Spinal Cord Repair DOI
Min Ho Han,

Hongru Yang,

Xiangdong Lu

et al.

Nano Letters, Journal Year: 2022, Volume and Issue: 22(15), P. 6391 - 6401

Published: July 25, 2022

Exosomes derived from mesenchymal stem cells (MSCs) have been proven to exhibit great potentials in spinal cord injury (SCI) therapy. However, conventional two-dimensional (2D) culture will inevitably lead the loss of stemness MSCs, which substantially limits therapeutic potency MSCs exosomes (2D-Exo). three-dimensional (3D-Exo) possess higher efficiency wide applications Typically, exosome therapy that relies on local repeated injection results secondary and low efficiency. It is urgent develop a more reliable, convenient, effective delivery method achieve constant situ release. Herein, we proposed controlled 3D-exohydrogel hybrid microneedle array patch SCI repair situ. Our studies suggested with 3D-culturing could maintain their stemness, consequently, 3D-Exo effectively reduced SCI-induced inflammation glial scarring. Thus, it promising strategy for treatment SCI.

Language: Английский

Citations

144
Mesenchymal stem cells-derived exosomes for drug delivery DOI Creative Commons
Yao Sun, Guoliang Liu, Kai Zhang

et al.

Stem Cell Research & Therapy, Journal Year: 2021, Volume and Issue: 12(1)

Published: Oct. 30, 2021

Abstract Exosomes are extracellular vesicles secreted by various cells, mainly composed of lipid bilayers without organelles. In recent years, an increasing number researchers have focused on the use exosomes for drug delivery. Targeted delivery in body is a promising method treating many refractory diseases such as tumors and Alzheimer's disease (AD). Finding suitable carrier has become popular research today. studies, mesenchymal stem cells (MSC-EXOs) been broadly researched due to their immune properties, tumor-homing elastic properties. While MSC-EXOs apparent advantages, some unresolved problems also exist. This article reviews studies delivery, summarizes characteristics MSC-EXOs, introduces primary production purification methods loading provide solutions existing suggestions future studies.

Language: Английский

Citations

128
Exosomes derived from 3D-cultured MSCs improve therapeutic effects in periodontitis and experimental colitis and restore the Th17 cell/Treg balance in inflamed periodontium DOI Creative Commons
Yong Zhang, Jiayao Chen, Haijun Fu

et al.

International Journal of Oral Science, Journal Year: 2021, Volume and Issue: 13(1)

Published: Dec. 1, 2021

Although mesenchymal stem cell-derived exosomes (MSC-exos) have been shown to therapeutic effects in experimental periodontitis, their drawbacks, such as low yield and limited efficacy, hampered clinical application. These drawbacks can be largely reduced by replacing the traditional 2D culture system with a 3D system. However, potential function of MSC-exos produced (3D-exos) periodontitis remains elusive. This study showed that compared generated via (2D-exos), 3D-exos enhanced anti-inflammatory ligature-induced model restoring reactive T helper 17 (Th17) cell/Treg balance inflamed periodontal tissues. Mechanistically, exhibited greater enrichment miR-1246, which suppress expression Nfat5, key factor mediates Th17 cell polarization sequence-dependent manner. Furthermore, we found recovery periodontium local injection attenuated colitis. Our not only through miR-1246/Nfat5 axis, improved treatment but also it provided basis for treating inflammatory bowel disease (IBD) immune responses periodontium.

Language: Английский

Citations

114
Technological advances towards extracellular vesicles mass production DOI Creative Commons

Alice Grangier,

Julien Branchu,

Jeanne Volatron

et al.

Advanced Drug Delivery Reviews, Journal Year: 2021, Volume and Issue: 176, P. 113843 - 113843

Published: June 17, 2021

Language: Английский

Citations

111
Manufacturing Therapeutic Exosomes: from Bench to Industry DOI Open Access
So-Hee Ahn, Seung-Wook Ryu, Hojun Choi

et al.

Molecules and Cells, Journal Year: 2022, Volume and Issue: 45(5), P. 284 - 290

Published: May 1, 2022

formulation stages, production under Good Manufacturing Practice (GMP) conditions for clinical use, and close consultation with regulatory authorities.

Language: Английский

Citations

91