Exosome-like vesicles encapsulated with specific microRNAs accelerate burn wound healing and ameliorate scarring DOI Creative Commons
Zhiyong Lei, Xiaojuan Chen,

Kezhuo Chen

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 2, 2025

Burn injuries are prevalent, yet effective treatments remain elusive. Exosomes derived from mesenchymal stem cells (MSC-Ex) possess remarkable pro-regenerative properties for wound healing. Despite their potential, the challenge of mass production limits clinical application. To address this, preparing exosome-like vesicles has become an international trend. In this study, 28 key microRNAs (miRNAs) with significant pro-proliferation, anti-inflammation, and anti-fibrosis functions were screened MSC-Ex. These miRNAs encapsulated into liposomes then hybridized extracellular watermelon to create synthetic vesicles. The fabricated exhibited similar particle size zeta potential MSC-Ex, demonstrating high serum stability effectively resisting degradation miRNA by RNase. They efficiently internalized enabled a rate lysosomal escape post cellular uptake, thereby exerting pro-proliferative, anti-inflammatory, anti-fibrotic functions. Further experiments demonstrated that these accelerated burn healing reduced scarring, effects comparable those natural Based on findings, in study present promising alternative MSC-Ex treatment.

Language: Английский

Endothelial progenitor cell derived exosomes mediated miR-182-5p delivery accelerate diabetic wound healing via down-regulating PPARG DOI Creative Commons
Peng Li,

Guanhao Hong,

Weiqiang Zhan

et al.

International Journal of Medical Sciences, Journal Year: 2023, Volume and Issue: 20(4), P. 468 - 481

Published: Jan. 1, 2023

Diabetic wound is one of the most common and serious complications diabetes, which characterized by abnormal number quality repair related cells. Previous studies have shown that human endothelial progenitor cells derived exosomes (EPCs-EXO) can promote diabetic healing through modulating vascular cell function. The purpose this study was to investigate biological effects molecular mechanisms EPCs-EXO on healing. regulation immortalized epidermal line HaCaT in high glucose (HG) environment evaluated. Our data showed promoted proliferation, migration, while inhibited apoptosis HaCaTs challenged HG via elevating miR-182-5p expression level vitro. Skin significantly enhanced mice. Moreover, bioinformatics analyses luciferase reporter assay indicated exosomal bound PPARG 3' UTR sequence PPARG. Collectively, our findings provided a new role clinical treatment skin wounds.

Language: Английский

Citations

20
The developing role of extracellular vesicles in autoimmune diseases: special attention to mesenchymal stem cell-derived extracellular vesicles DOI
Geovanny Genaro Reiván Ortiz,

Neelam Hazoor Zaidi,

Ravinder Saini

et al.

International Immunopharmacology, Journal Year: 2023, Volume and Issue: 122, P. 110531 - 110531

Published: July 10, 2023

Language: Английский

Citations

20
Bacterial cellulose as a potential biopolymer for wound care. A review DOI

Farzaneh Jabbari,

Valiollah Babaeipour

International Journal of Polymeric Materials, Journal Year: 2023, Volume and Issue: 73(6), P. 455 - 477

Published: Jan. 18, 2023

Different types of wound dressings have been introduced in various forms such as membrane, gel, sponge, and hydrocolloid. Bacterial cellulose (BC) is a natural polymer produced by many bacterial cells. It has received much attention wound-healing due to its attractive suitable properties including purity, ultrafine network structure, high water holding capacity, excellent mechanical properties, cytocompatibility, crystallinity. In this review, the healing process first described, then their advantages disadvantages are discussed. Also, BC-based combination with other materials investigated for antimicrobial activity.

Language: Английский

Citations

18
Treatment strategies for intrauterine adhesion: focus on the exosomes and hydrogels DOI Creative Commons

Fengling Wu,

Ningjing Lei, Shenyu Yang

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: Aug. 31, 2023

Intrauterine adhesion (IUA), also referred to as Asherman Syndrome (AS), results from uterine trauma in both pregnant and nonpregnant women. The IUA damages the endometrial bottom layer, causing partial or complete occlusion of cavity. This leads irregular menstruation, infertility, repeated abortions. Transcervical electroreception (TCRA) is frequently used treat IUA, which greatly lowers prevalence adhesions increases pregnancy rates. Although surgery aims disentangle adhesive tissue, it can exacerbate development when degree severer. Therefore, critical develop innovative therapeutic approaches for prevention IUA. Endometrial fibrosis essence studies have found that use different types mesenchymal stem cells (MSCs) reduce risk increase possibility pregnancy. Recent research has suggested exosomes derived MSCs overcome limitations MSCs, such immunogenicity tumorigenicity risks, thereby providing new directions treatment. Moreover, hydrogel drug delivery system significantly ameliorate recurrence rate intrauterine patients, its potential mechanism treatment been studied. It shown combination two more schemes broader application prospects; therefore, this article reviews pathophysiology current strategies, focusing on combined with hydrogels certain challenges treating they still provide promising field.

Language: Английский

Citations

18
Recombinant human collagen I/carboxymethyl chitosan hydrogel loaded with long-term released hUCMSCs derived exosomes promotes skin wound repair DOI
Qiong Wu,

Yayuan Guo,

Hongwei Li

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 265, P. 130843 - 130843

Published: March 12, 2024

Language: Английский

Citations

8
Insights into optimizing exosome therapies for acute skin wound healing and other tissue repair DOI

Tianjing Sun,

Mo Li,

Qi Liu

et al.

Frontiers of Medicine, Journal Year: 2024, Volume and Issue: 18(2), P. 258 - 284

Published: Jan. 13, 2024

Language: Английский

Citations

6
Characterization of cellular senescence in radiation ulcers and therapeutic effects of mesenchymal stem cell-derived conditioned medium DOI Creative Commons
Wanchao Chen, Yang Wang, Jiancheng Zheng

et al.

Burns & Trauma, Journal Year: 2023, Volume and Issue: 11

Published: Jan. 1, 2023

Abstract Background Radiation ulcers are a common and severe injury after uncontrolled exposure to ionizing radiation. The most important feature of radiation is progressive ulceration, which results in the expansion nonirradiated area refractory wounds. Current theories cannot explain progression ulcers. Cellular senescence refers as irreversible growth arrest that occurs stress, contributes tissue dysfunction by inducing paracrine senescence, stem cell chronic inflammation. However, it not yet clear how cellular facilitates continuous Here, we aim investigate role promoting indicate potential therapeutic strategy for Methods ulcer animal models were established local 40 Gy X-ray continuously evaluated >260 days. roles assessed using pathological analysis, molecular detection RNA sequencing. Then, effects conditioned medium from human umbilical cord mesenchymal cells (uMSC-CM) investigated models. Results with features clinical patients primary mechanisms responsible We have characterized being closely associated found exogenous transplantation senescent significantly aggravated them. Mechanistic studies sequencing suggested radiation-induced secretions facilitating Finally, uMSC-CM was effective mitigating inhibiting senescence. Conclusions Our findings only characterize but also their treatment.

Language: Английский

Citations

15
Biomimetic scaffolds loaded with mesenchymal stem cells (MSCs) or MSC-derived exosomes for enhanced wound healing DOI Creative Commons
Alireza Ghasempour, Hamideh Dehghan, Mahmoud Mahmoudi

et al.

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 9, 2024

Since wound healing is one of the most important medical challenges and common dressings have not been able to manage this challenge well today, efforts increased achieve an advanced dressing. Mesenchymal stem cells exosomes derived from them shown high potential in regenerating wounds due their immunomodulatory, anti-inflammatory, immunosuppressive, regenerative capacities. However, such as short life these cells, low durability area, stability resulted limitations use for healing. Nowadays, different scaffolds are considered suitable biomaterials These made natural or synthetic polymers promising ideal dressing that does disadvantages dressings. One strategies has attracted much attention today using seeding delivering MSCs exosomes. This combined strategy a enhancing shelf increasing In review, combination with comprehensively discussed.

Language: Английский

Citations

5
Knockdown of deleterious miRNA in progenitor cell–derived small extracellular vesicles enhances tissue repair in myocardial infarction DOI Creative Commons
Hyun‐Ji Park, Jessica R. Hoffman, Milton E. Brown

et al.

Science Advances, Journal Year: 2023, Volume and Issue: 9(9)

Published: March 3, 2023

Small extracellular vesicles (sEVs) play a critical role in cardiac cell therapy by delivering molecular cargo and mediating cellular signaling. Among sEV molecule types, microRNA (miRNA) is particularly potent highly heterogeneous. However, not all miRNAs are beneficial. Two previous studies using computational modeling identified miR-192-5p miR-432-5p as potentially deleterious function repair. Here, we show that knocking down c-kit + (CPC)–derived sEVs enhances the therapeutic capabilities of vitro rat vivo model ischemia reperfusion. miR-192-5p– miR-432-5p–depleted CPC-sEVs enhance reducing fibrosis necrotic inflammatory responses. miR-192-5p–depleted also mesenchymal stromal cell–like mobilization. Knocking from could be promising strategy for treatment chronic myocardial infarction.

Language: Английский

Citations

13
Exosomes in the Treatment of Pancreatic Cancer: A Moonshot to PDAC Treatment? DOI Open Access
Stavros P. Papadakos, Nikolaos Dedes, Alexandros Pergaris

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(7), P. 3620 - 3620

Published: March 25, 2022

Pancreatic Ductal Adenocarcinoma (PDAC) constitutes a leading cause of cancer death globally. Its mortality remains unaltered despite the considerable scientific progress made in fields diagnostics and treatment. Exosomes comprise small extracellular vesicles secreted by nearly all cells; their cargo contains vast array biomolecules, such as proteins microRNAs. It is currently established that role messengers central to plethora both physiologic pathologic processes. Accumulating data have shed light on contributions carcinogenesis, metastasis, immunological response. Meanwhile, advancement personalized targeted therapies into everyday clinical practice necessitates development cost-efficient treatment approaches. The exosomes being extensively investigated towards this direction. This review aims summarize current pre-clinical evidence regarding effects exosomal applications timely diagnosis, prognosis, therapeutic management pancreatic cancer.

Language: Английский

Citations

18